Supplementary Figure S1. Visualization of the stratified-seed germination after three diurnal cycles of dehydration (Dh 1, Dh 2, Dh 3) compared with seeds germinated following stratification (St) for four Arabidopsis ecotypes
Supplementary Figure S2. Fatty acid profile, as percentage of total FAs of lipids extracted from seeds of four Arabidopsis ecotypes through three continuous hydration-dehydration cycles, Error bars represent mean ± SE.
16:0
18:0
18:1n-9
18:2n-6
18:3n-3
20:1n-9
Col-0 Cvi C24 LerP
erce
ntag
e of
tot
al F
As
(%)
Supplementary Figure S3. Malondialdehyde (MDA) equivalents (µmol/g) recovered from Arabidopsis seeds of four ecotypes following repetitive dehydrations. Error bars represent mean ± SE of 3 independent measurements.
Col-0 Cvi C24 Ler
MD
A c
onte
nt (
μm
ol/g
)
Supplementary Figure S4. Partial Least Squares - Discriminant Analysis (PLS-DA) of ecotypic differences across Hy-Dh cycles. 3D score plot between the selected PCs and corresponding explained variances are shown.
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Cvi
C24LerCol-0
Cvi
C24LerCol-0
Cvi
C24LerCol-0
Cvi
C24LerCol-0
Cvi
C24LerCol-0
Cvi
C24LerCol-0
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C24LerCol-0
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C24LerCol-0
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C24Ler
Dry St Hy 1 Hy 2 Hy 3 Hy 4Dh 3Dh 2Dh 1
0 0.4 0.8
Supplementary Figure S5. False scale color visualization of significant (p<0.05, ANOVA) metabolic difference among ecotypes at each experimental time point tested. False scale color represents the relative intensity normalized by the median of the selected metabolites across all the time points followed by log transformation (base 10), and expressed as the mean of three independent replicas. Abbreviation : Glc-1,6-AH, glucose-1,6-anhydro,beta; Myo-inositol-1-P, myo-inositol-1-phosphate; PME, phosphorate-monomethyl ester; Glycerol-3-P, glycerol-3-phosphate; GPG, glycerophosphoglycerol; Gluconate-1,5-L, gluconate-1,5-lactone.
Col-0 Cvi C24 Ler
Col-0
Cvi
C24
Ler
Supplementary Figure S6. Relative yield of the reduced Triphenyl tetrazolium chloride (TTC) by TCA/acetone extraction. Absorbance was plate assayed at 485 nm. The extraction absorbance was measured (left). TTC reduction is reflected by the change in color of the extract (right). Values are presented by the mean of five independent preparations ± SE.
Supplementary Figure S7. Protein content of seeds after three hydration-dehydration cycles compared with that after stratification. Values are means of four independent preparations. Error bars represent mean ± SE
Col-0 Cvi C24 Ler
