\'li'rWfTrq:; qf;m ;:p:f /3 \'lfcn 2 RW<n: 2005 ';[ . /32-/37 w c@ c@ q;mTq)Fm lR q:z;i) {I Cf)T n;Jf Cl)!1R \WcfT* lJ;CT J::!; '" 0111WT, fcr:r 3qB'Cff.l rfR:c <lTCR1 80 1lim '1Pf, - 226 001 : Wi i'f c@ \3GFlT i'f qrm \ifRlT I 3WfTCIT fcrfl:Fr ;J'R'r m, mom C!itrT, tc 3rrR i'f ' 4'r qT{ / 0 r:r4'r ( fl <fiT cmor / 3 ITucnl1Rf crrB ;JWT <J / \lCflR (fl q<: Mir (1cI) ;JWT \9Rf cf; m ctcl 0ffi:.:>:r Wr i r;r c@ gqc;r B' !WRT / i'f qR q<: c@ gr$'rco 01Br c@ 3m 31Br i'f crrir J1R (fM'r<:r iRt / i'f W c@ 31Br c@ (. q<: tilEwr c@ fm'1'Ff BRClf0iT cf; g'11fCT q<: STill rrm / c@ fJ:F.1-fl:r;:;, BRClf tffi;i'f <fiT cnt TR; WiT c@ 0 1Br cf; i'f '1RCfi1 \'ffi1 fiRe: Fc8:rr rrm, fit( 3m cf; 3fn: 3A CfiT Fc8:rr rrm / 3 WfTC1T, mqp::r WiT c@ 3 1Br (. m cf; \f.lC& q<: CfiT g'11fCT '1ft 00 rrm / qRIJI14Ij{1l ( i'f c@ BRClf cf; i'f c@ m <fiT Cli1ill % I tfr i'f qT{ CfiT m:r c@ q;fiZ cmor / c@ Ca 2 + -Mg2+ - ATPase i'f '1ft BRClf \31TmRo m:r qrm rrm / \3Uf BRClf mr 41INi)R;J[{jg c@ f.i1fc'Rr 'm qT{ / c@ 31Br cf; (. CFimr1 q<: frr?jWIlfOT qfturrl:JT";t crffi cf; CfiT m:r fcl:; BRClf-R'm m / \lCflR % qrm rrm fcl:; c@ \3Uf BRClf 0 1Br c@ c8 ClWfT 3 1Br cf; <fiT 'qffi % / Influence of Fluoride on Rat Intestinal Bacteria and Epithelial Cells Raj K Upreti & A Kannan Biomembrane Division, Industrial Tox ic ology Research Centre, P. O. Box 80 M G Marg, Lucknow - 226001 Abstract Flu orides occ ur naturally in th e earths' cru st where they are found in rock s, coal, clay and soil. They are released into th e air and water al so from indu stri es like coal-fired power pl ant s, aluminium sme lt ers, fertili ze r plant s, glass, brick and tile wo rk s and plasti c factories. Chronic fluoride ingestion can lead to ske let al fluorosis, teeth molting, gastro intestinal pain, nausea and vo mit ing. Following oral adm in istration, the prime si te of int e ra cti on is th e epithelium of intestine and th e gut mi cro fl ora. In th e present stud y, th e ill situ effect of sodium fluoride was carried ou t on rat intestinal epithelial ce ll linin g, and th e ill vitro effec t on a facult ati ve gut bacteria Ecoli. In testinal loo ps were fill ed with sodium tluoride solutions (0,24,48 and 96 mM ) and in cubated in situ for 30 min . Results revealed a concentra ti on-depe nd en t in crease of alkaline ph os ph atase and cholesterol in th e luminal tluid indicating th e release of enzyme and sterol from the epithe li al ce il membran e. Concomitant dec lin e th ese in the brush border prepara ti ons we re al so ev id ent. Ca 2 +- Mg2+_ ATPase ac tiviti es of bru sh border al so found to be declin ed . III vitro ef fect of fl uoride on E coli also revea l ed a concentration-dependent decline in grow th parameters. These res ult s indi cate substantial damage to th e epithelial ce il membrane and gut Ecoli with hi gher tluoride concen tr ati ons. t! Author for correspondence
Influence of Fluoride on Rat Intestinal Bacteria and Epithelial Cells
Raj K Upreti & A Kannan Biomembrane Di vis ion, Industri al Tox icology Research Centre, P. O. Box 80
M G Marg, Lucknow - 226001
Abstract
Fluorides occur naturally in the earths' crust where they are found in rocks, coal, clay and soil. They are released into the air and water also from industri es like coal-fired power pl ants, aluminium smelters, fertili zer plants, glass, brick and tile works and plastic factor ies. Chronic fluoride ingestion can lead to skeletal fluorosis, teeth molting, gastro intestinal pain, nausea and vomit ing. Following oral adm in istration, the prime si te of interaction is the epithelium of intestine and the gut micro fl ora. In the present study, the ill situ effect of sodium fluoride was carried out on rat intestinal epithelial ce ll lining, and the ill vitro effec t on a facultati ve gut bacteria Ecoli. In testinal loops were fill ed with sodium tluoride solutions (0,24,48 and 96 mM ) and incubated in situ for 30 min . Results revealed a concentration-dependent increase of alkaline phosphatase and cholesterol in the luminal tluid indicating the release of enzyme and sterol from the epitheli al ceil membrane. Concomitant decline these in the brush border preparati ons were also ev ident. Ca2+- Mg2+_ ATPase ac tiviti es of brush border also found to be declined . III vitro effect of fl uoride on E coli also revealed a concentration-dependent decline in growth parameters. These results indicate substantial damage to the epithelial ceil membrane and gut Ecoli with higher tluoride concen trations.
*~ t! ~I Author for correspondence
5 o .O\~k ,Pta;:e "
4 c EJC a.!I.1g-ATP:!se Q)
,"'-' 0
3 I..-0. D)
E 2 --\/)
.-::! C ::>
1
0 I) 24 48
NaF(rrM)
fi:T:11- ¥c& jmcF;~~T<nP~lR~~ COT 'r'lT'<f I c:~Tfu: rm: ;jTt<l% qiT:f ~ lR m cF; ;jffiRl" ± 11TTcfi
~ (m ~ ± ~ ~~H) lR ;jTT~.nfuT %' I
!>ittilcHI
~ if ~~~1{1~5 fcR:IT;r fcR:IT ffl if~, ~ eFt ~ jm fqW if qmr \JfRIT % 1 ~ W2:f zq ~, \JWr,
~-~\3rr ~. ~-qTm if ~ ~~ ~-m ~ % 1
~ IR ~ CDr QOcruq'I\j:H i:r:r-\JWr, <:9m-~, qfkBI~~B, c:cm:t (fcr~rsrcr: G'd Rlf<h,BI ~m) 0nf?; B' iTcrr % 1 cnt qTtT ~ 'qfI:f, thl'Ht~5 ctr \3mrcn ~ m ~ 1 \31\1: \3~ 'qfI:f cfi 00- B' ~'11\1~5 eFt ~liR if ~ ~ B'11cf % I Cf)I1
w;T cFr 0:rR if q1<T \Jl'R cr8 m ~Cf~ RI:I I t. ~ cnT 'r21CO m ~~ fcnm 11m I t. ~ ct ~ Bcrq;, (~) c8 ~ Lk'11{I~s cFr ~ ~01T ct m:a ~-\J1"C'l' lTIv:r1 (~) if R1Chmd fctm 11m I
