1.Medical Purposes2.Reviving endangered species and extinct species3.Reproducing a deceased pet4.Cloning humans?????

http://www.cartoonstock.com/newscartoons/cartoonists/mba/lowres/mban1450l.jpg

Mysteries that can be solved by DNA•Crimes (murder, rape, robbery, etc.)•Human Identification•Prove Kinship (paternity, maternity, etc..•Prove innocence•Genetic disorders•Historical mysteries•Evolution trends

By comparing DNA fingerprints of the unknown to the known

Samples are created by gel electrophoresis

also called DNA testing, DNA typing, or DNA profiling

is a technique used to distinguish between individuals of the same species using only

samples of their DNA

Using a lysis solution and a warm water bath

Using an ultracentrifuge(separated based on density)

Using forms of alcohol to clumpDNA

Process of removing DNA from cells received from samples of blood, skin, semen, etc.

piece of equipment, generally driven by a motor, that puts

an object in rotation around a fixed axis

The centrifuge works using the sedimentation principle

where the centripetal acceleration is used to separate substances of

greater and lesser density

Process in which DNA is “cut” by restriction enzymes into fragments

Restriction Enzymes are enzymes that cut DNA at SPECIFIC sequences of nucleotides.

is a technique used for the separation of DNA, RNA, or protein molecules using an

electric current applied to a gel matrix, based

on their size and electriccharge

Gel electrophoresis

gel animation

• To determine differences in the genomes of different plant and animal species

• The number and positions of the bands formed on each lane of gel is the actual DNAfingerprint and is unique to each person

DNA EXTRACTION

DNA FRAGMENTATION

GEL ELECTROPHORESIS

Using your notes and your knowledge of DNA fingerprinting, make a list of materials

and tools needed for us to perform a paternity test?

http://www.youtube.com/watch?v=-NMHGkOBXic

Lysis solution

DNA samples

ultracentrifuge

Restriction enzymes

Gel chamber

Power supplypaternity test

Alcohol(s)

Warm water bath