Ded

icat

ed A

naly

tica

l Sol

utio

ns

RECE

NT

ADVA

NCE

S IN

FTI

R DH

I Sem

inar

TLR

I, Ta

iwan

15

Oct

ober

201

5 St

een

Kold

-Chr

isten

sen,

Inte

rnat

iona

l Mar

ket M

anag

er, F

OSS

, Den

mar

k

Ded

icat

ed A

naly

tica

l Sol

utio

ns

2 2

AGEN

DA

Infra

red

anal

ysis

of m

ilk

Mid

infra

red

spec

tros

copy

M

easu

rem

ent o

f min

or c

ompo

nent

s

Targ

eted

and

non

-targ

eted

ana

lyse

s

A v

ision

of f

utur

e ap

plic

atio

ns

Ded

icat

ed A

naly

tica

l Sol

utio

ns

3

MID

IR S

PECT

RA O

F LI

QU

ID M

ILK

0.0

0.1

0.2

0.3

0.4 1

00

01

10

01

20

01

30

01

40

01

50

01

60

0

wav

enu

mb

er

absorbance

Ded

icat

ed A

naly

tica

l Sol

utio

ns

4

MIL

K AD

ULT

ERAT

ION

0.0

0.1

0.2

0.3

0.4 1

000

1100

1200

1300

1400

1500

1600

wave

num

ber

absorbance

am

moniu

m s

ulp

hate

ure

am

ela

min

e

Ded

icat

ed A

naly

tica

l Sol

utio

ns

5 5

WH

AT C

AN F

T-IR

OFF

ER U

S TO

DAY?

MIR

spec

tros

copy

has

sinc

e 19

70’s

offe

red

rapi

d an

alys

is of

mai

n co

nstit

uent

s fa

t, pr

otei

n, la

ctos

e, so

lids &

solid

s-no

n-fa

t

FT-IR

spec

tros

copy

is th

e st

ate-

of-t

he-a

rt m

etho

d fo

r ac

quiri

ng M

IR sp

ectr

a an

alyz

ing:

Co

nven

tiona

l par

amet

ers

fat,

prot

ein

(tru

e &

cru

de),

case

in, l

acto

se, s

olid

s, u

rea,

citr

ic

acid

, fre

e fa

tty

acid

s, P

H, F

PD

New

par

amet

ers

Fatt

y Ac

ids P

rofil

ing

Keto

sis sc

reen

ing

(BHB

and

Ace

tone

) Ad

ulte

rant

s Sc

reen

ing

Ded

icat

ed A

naly

tica

l Sol

utio

ns

6

THE

USE

OF

TARG

ETED

& U

NTA

RGET

ED

CALI

BRAT

ION

MO

DEL

S

Mel

amin

e cr

isis (

2008

) with

cont

amin

ated

milk

pow

der

Liqu

id m

ilk d

elib

erat

ely

adul

tera

ted

for e

cono

mic

gai

n Co

llabo

rativ

e pr

ojec

t bet

wee

n Fo

nter

ra, A

rla a

nd F

OSS

Re

sulte

d in

dev

elop

men

t of t

arge

ted

and

unta

rget

ed m

odel

s for

det

ectin

g m

ilk

adul

tera

tion

at e

cono

mic

leve

ls

In 2

014

we

have

rele

ased

FTI

R m

odel

s usin

g ab

norm

al sp

ectr

um m

odul

e (A

SM) a

s wel

l as

targ

eted

mod

els f

or sp

ecifi

c ad

ulte

rant

s

Ded

icat

ed A

naly

tica

l Sol

utio

ns

7

FOSS

CO

MM

ITM

ENT

IN T

ESTI

NG

AG

AIN

ST M

ILK

ADU

LTER

ATIO

N

FOSS

star

ted

in th

e ni

netie

s with

Infra

red

anal

ysis

agai

nst s

impl

e an

d k

now

n ad

ulte

rant

s. S

uch

as te

sts

for s

cree

ning

for a

dded

wat

er.

As th

e ad

ulte

ratio

n sc

hem

es b

ecam

e m

ore

com

plex

FO

SS d

evel

oped

new

tool

s for

mak

ing

scre

enin

g m

odel

s aga

inst

unk

now

n ad

ulte

rant

s.

Toda

y w

e of

fer ”

out o

f the

box

” sc

reen

ing

mod

el

agai

nst a

bnor

mal

ities

in th

e m

ilk a

s wel

l as o

fferin

g m

any

targ

eted

cal

ibra

tions

aga

inst

spec

ific

adul

tera

nts.

With

in 3

0 se

c th

e da

iry p

lant

can

scre

en th

e m

ilk fo

r an

y un

know

n ad

ulte

rant

s or a

naly

ze fo

r wel

l-kno

wn

adul

tera

nts s

uch

as M

elam

ine

or C

IP a

gent

by

mea

ns

of in

frare

d an

alyz

ers

Ded

icat

ed A

naly

tica

l Sol

utio

ns

8

Milk

qua

lity

is te

sted

bef

ore

unlo

adin

g.

But m

ilk a

dulte

ratio

n is

rare

ly te

sted

at

the

plat

form

.

Milk

Adu

ltera

tion

is a

grow

ing

prob

lem

.

Adut

erat

ion

can

be d

elib

erat

e or

cau

sed

by a

ccid

ent.

MIL

K AD

ULT

ERAT

ION

Ded

icat

ed A

naly

tica

l Sol

utio

ns

9

SCRE

ENIN

G F

OR

MIL

K AD

ULT

ERAT

ION

Uni

nten

tiona

l dev

iatio

ns

Caus

ed b

y ac

cide

nts o

r fai

lure

s.

Agen

t in

the

cow

s fee

d th

at is

tran

spor

ted

on to

the

milk

Cl

eani

ng a

gent

W

ater

In

tent

iona

l dev

iatio

ns

Driv

en b

y ec

onom

ic g

ains

:

amou

nt (w

eigh

t and

/or v

olum

e)

milk

fat c

onte

nt

milk

pro

tein

con

tent

dr

y m

atte

r con

tent

th

e to

tal b

acte

rial c

ount

s and

som

atic

cel

l cou

nts

Ded

icat

ed A

naly

tica

l Sol

utio

ns

10

NO

RMAL

VS

ABN

ORM

AL S

PECT

RUM

FTIR

spec

tra

from

nat

ural

raw

milk

sam

ples

is a

uni

que

finge

r prin

t of n

orm

al m

ilk

Raw

milk

sam

ple

Ded

icat

ed A

naly

tica

l Sol

utio

ns

11

11

UN

TARG

ETED

SCR

EEN

ING

MO

DEL

You

can

scre

en a

ll m

ilk sa

mpl

es a

gain

st a

bnor

mal

ities

. It

will

not

tell

you

wha

t is i

n th

e m

ilk o

nly

if it

is no

rmal

milk

or n

ot.

Adva

ntag

es u

ntar

gete

d m

odel

allo

ws y

ou sc

reen

ing

for a

n un

limite

d nu

mbe

r of u

nkno

wn

pote

ntia

l ad

ulte

rant

s with

in 1

0 - 3

0 se

c.

Ded

icat

ed A

naly

tica

l Sol

utio

ns

12

READ

Y TO

USE

MO

DEL

FO

R TH

E M

ILKO

SCAN

FT1

Read

y to

use

mod

el to

scre

en

agai

nst a

bnor

mal

milk

in ra

w c

ow

milk

.

Befo

re st

artin

g th

e sc

reen

ing

of

your

milk

you

nee

d to

con

sider

; N

umbe

r of f

alse

pos

itive

and

ne

gativ

e re

sults

How

to m

ake

conf

irmat

ory

test

s

Wha

t to

do w

ith th

e m

ilk in

the

truc

k

Ded

icat

ed A

naly

tica

l Sol

utio

ns

13

The

outp

ut fr

om th

e m

odel

is a

n AS

M S

core

.

If AS

M sc

ore>

thre

shol

d, th

e ne

w

sam

ple

is cl

assif

ied

as a

bnor

mal

NU

MBE

R O

F FA

LSE

POSI

TIVE

AN

D

NEG

ATIV

E RE

SULT

S

2 4 6 8

True

Pos

itive

(adu

ltera

ted)

False

Pos

itive

(not

adu

ltera

ted)

True

Neg

ativ

e (n

ot a

dulte

rate

d)

False

Neg

ativ

e (a

dulte

rate

d)

Thre

shol

d =

3

ASM score D

edic

ated

Ana

lyti

cal S

olut

ions

14

The

grap

h sh

ows t

he e

ffect

of

chan

ging

the

thre

shol

d in

the

mod

el.

A th

resh

old

on 3

cor

resp

onds

to

a fa

lse p

ositi

ve ra

te o

f 1.5

%

whe

reas

a th

resh

old

on 5

co

rres

pond

s to

a fa

lse p

ositi

ve

rate

of 0

.4%

.

NU

MBE

R O

F FA

LSE

POSI

TIVE

AN

D N

EGAT

IVE

RESU

LTS

24

68

1001234

Thre

shol

d

Percentage of false positives

Ded

icat

ed A

naly

tica

l Sol

utio

ns

15

WH

AT C

AN B

E SC

REEN

ED A

GAI

NST

W

ITH

TH

E U

NTA

RGET

ED M

OD

EL ?

The

tabl

e sh

ows t

he L

imit

of D

ecte

ctio

n fo

r the

adu

ltera

nts w

hich

cou

ld b

e de

tect

ed a

s abn

orm

al b

y th

e m

odel

.

The

LoD´

s cor

resp

ond

to a

thre

shol

d of

3.

Adul

tera

nt

LoD

L

oD A

dulte

rant

Lo

D L

oD A

dulte

rant

Lo

D

Alla

ntoi

n

300

ppm

C

yanu

ric a

cid

17

0 pp

m

Sod

ium

bic

arbo

nate

40

0 pp

m

Am

idou

rea

5

00 p

pm

Cyr

omaz

ine

30

0 pp

m

Sod

ium

hyd

roxi

de

0.06

%

Am

inot

riazin

e 8

00 p

pm

Dic

yand

iam

ide

30

0 pp

m

Sod

ium

nitr

ite

200

ppm

Am

mon

ium

nitr

ate

20

0 pp

m

For

mal

dehy

de

400

ppm

T

hiou

rea

50

0 pp

m

Am

mon

ium

sulp

hate

30

0 pp

m

Hyd

roxy

prol

ine

90

0 pp

m

Triu

ret

800

ppm

Biu

ret

600

ppm

M

alto

dext

rine

11

00 p

pm

3-a

min

otria

zole

11

00 p

pm

CIP

age

nt

1%

Mel

amin

e 3

00 p

pm

4-

amin

otria

zole

14

00 p

pm

Ded

icat

ed A

naly

tica

l Sol

utio

ns

16

Scre

en fo

r al

l unk

now

n ad

ulte

rant

s

Scre

en fo

r sp

ecifi

c ad

ulte

rant

s

SECU

RITY

SCR

EEN

ING

IN 3

0 SE

CON

DS

Rapi

d in

expe

nsiv

e sc

reen

ing

of y

our m

ilk

Conf

irmat

ory

test

Actio

n pl

an

Secu

re d

airy

pr

oduc

ts

Unt

arge

ted

mod

el

Targ

eted

m

odel

s

Ded

icat

ed A

naly

tica

l Sol

utio

ns

17

FOSS

GO

OD

PRO

DU

CT

- A S

TRO

NG

VIS

ION

Tool

s to

secu

re a

n ef

fect

ive

and

safe

supp

ly c

hain

Scre

en th

e fe

ed fo

r abn

orm

aliti

es

Scre

en th

e m

ilk &

dai

ry p

rodu

ct

for a

bnor

mal

ities