2011 | 2012 Annual Report

Creating KnowledgeDeveloping Cures

McLaughlin Research Institute

CONTENTS

Message from the Director . . . . . . . . . . . . . . . . . . . . . . . . . 2

MRI’s Progress In Sync with National Plan to Address Alzheimer’s Disease . . . . . . . . . . . 4

Learning to Cope with Parkinson’s Disease . . . . . . . . . . . . . 6

MRI’s Model Science Education Program . . . . . . . . . . . . . . 8

Former MRI Graduate Student Advances Alzheimer’s Research . . . . . . . . . . . . . . . . . . . . . 11

Scientists Learn, Share and Contribute . . . . . . . . . . . . . . 12

Scientific Publications . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Seminars . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

Staff . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

Partners in Research . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

Help Support Research . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

Donors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

2011 - 2012 Financial Data . . . . . . . . . . . . . . . . . . . . . . . . 24

PUBLICATION CREDITS

Editor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Lisa Flowers, PhD

Writer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Janet Henderson

Designer . . . . . . . . . . . . . . . . . . Pam Lemelin, Renaissance Creative

Principal Photography . . . . . . . . . . . . . . . . . . . . . . . Darrin Schreder

Additional Photography . . . . . . . . . . . . Larry Beckner, Rion Sanders

Printing . . . . . . . . . . . . . . . . . . . . . . . . . . . . Advanced Litho Printing

Square Butte photo on back cover courtesy McMillan Studio

One of the many ways you can support our mission is by spreading

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The report is also available on our website:

mclaughlinresearch.org

Our MissionTo improve human health through innovative genetic research and education

Since 1954, scientists at McLaughlinResearchInstitute (MRI)have been working to defeat human diseases. Their researchhas made important contributions to the fights againstAlzheimer’s, Parkinson’s, diabetes, multiple sclerosis, hearingloss, and mad cow disease. Early research at the Institute was influential in the development of transplantation medicine. Today, the researchers are focused on brain diseases.

For more than 50 years, the Institute has nurtured young scientists-in-the-making through our internship program for high school and college students. Our education program also includes working with public schools to enhance science education.

“Our research at MRIis going to help patients.”

–Dr. George Carlson

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MRI in Pictures Stained sections of mouse brain tissue on microscope slides; McLaughlin’s mice are used throughout the worldto model disease for biomedical research; Dr. George Carlson, MRI Director; Jill O’Moore prepares mouse brain sections for microscopic detection of pathological changes similar to those in Alzheimer’s disease patients; MRI facility, Great Falls, MT.

In an exciting step into a new future of translational research, McLaughlin Research Institute is building a partnership with BenefisHealth System in Great Falls, Montana, thatpromises to carry the Institute’s research results into the realm of patient care. This development offers new hope for Montana’s agingpopulation, which faces a grim forecast in the nation’s Alzheimer’s epidemic.

To facilitate its entry into clinical medicine, MRI issearching for a Physician-Scientist who will see patients with degenerative brain diseases at Benefisand conduct research in a lab at the Institute, tying the two practices together to enhance both the research and the medicine. Physicians with the rarecombination of clinical expertise and a productive laboratory research program are not plentiful, and recruiting one will take some time, but MRI is eager to take this logical next step in the battle againstAlzheimer’s, Parkinson’s, and related diseases.

Translating basic research discoveries to clinical practice is one of the pillars of the National Plan to Address Alzheimer’s Disease and related disorders.The plan prioritizes the identification of biomarkers forthese diseases, which is a key component of ongoingresearch at MRI; please take a few minutes to readmore about this on page 4. The National Plan’s priorities dovetail seamlessly with McLaughlin’s

research, and the alliance with Benefis positions theInstitute to maximize its contribution to the fightagainst degenerative brain disease.

Eminent Stanford University researcher Dr. IrvingWeissman is the longtime chair of MRI’s Scientific Advisory Committee. His vision has been instrumentalin moving the Institute towards clinical medicine. “We have a rich history,” he said. “We have achance to make a bold leap so that we can notonly treat people but we can also be at the leadingedge of understanding their [families’] diseasesand part of the new way of translating medicine.”

Parkinson’s patients will ultimately benefit from translational research in ways that are similar to thosewith other degenerative brain diseases. This report includes the story of Dr. Paul Melvin of Helena andhis experience of living with this life-changing disease.

I hope you will enjoy reading about MRI’s science education program, which is one of the best in thecountry. The summer program for students begannearly 60 years ago and has evolved to include a highquality research experience for science teachers, followed by a second summer spent developing newcurriculum inspired by the research. Each year hundredsof students benefit from the enthusiasm and enrichedlearning opportunities their teachers bring back toclass from the Institute.

Message from the Director

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Among the many ways the Institute has nurtured thenext generation of scientists is a new one for MRI: Miranda Yeska-Orr was the first graduate student toconduct her doctoral research at McLaughlin, and lastyear she received her PhD and published her intriguingresearch findings in a peer-reviewed journal. Her storyin the following pages makes plain the vital roleMcLaughlin Research Institute continues to play in the lives of young Montanans.

Unfortunately, at the same time that degenerativebrain disorders threaten to touch every Americanfamily, at a perilous human and financial cost toboth individuals and the government, federalfunding for medical research is increasingly difficultto come by. This funding shortage puts independentresearch facilities like MRI in a precarious position.

In addition to those federal funds from the National Institutes of Health and various well aligned biomedicalcollaborations and contracts, the Institute has reliedheavily on the generosity of private donations throughoutits history. We are grateful for the gifts from our supportersthat help make it possible to carry on this importantwork. Please read in the following pages about recentgifts to MRI from individuals who used planned givingto help sustain McLaughlin’s mission. We hope youwill consider how you can help support research andeducation at the Institute.

Leading the Institute’s development efforts is our newDevelopment Officer, Lisa Flowers, PhD. MRI’s staffand board members are united in our enthusiasmabout Dr. Flowers, who came on board in mid June ofJune 2012. Lisa is a wonderful addition to the MRI team.

MRI was honored this year to develop a friendship withJoe and Patty Mazurek of Helena and was saddenedby Joe’s death from early-onset Alzheimer’s. The Mazurekfamily generously teamed up with the Institute to help advance a cure for this devastating disease by telling Joe’stragic story – of a former Montana attorney general whosecareer and life were cruelly cut short.

To help us communicate more effectively with you, oursupporters, and the larger public, MRI launched itsnew website and Facebook page in May. Visit us onthe web at mclaughlinresearch.org or on Facebookat McLaughlin Research Institute and keep in touch withour progress during this pivotal time. Our ambitious nextchapter – bridging basic research with clinical trials to benefit patients – will be possible with your transformational support. The MRI Board and staff sincerely thank you for choosing to be part of theevolving community-based story of McLaughlin Research Institute’s work to improve human health as we move into a bold and exciting future.

George Carlson, PhDDirector & Professor

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Partnerships A new connection with Benefis Health System will further translational research. Benefis CEO John Goodnow (left) is pictured with longtime collaborators Lee Hood, George Carlson and Irving Weissman.

Anew study has revealed that indicators of diseases like Alzheimer’scan be present in a person’s bodymore than 20 years before they showsigns of the disease. “It’s terrifying,” MRI director George Carlson said. “Tothink that a degenerative brain diseasemight be lurking in our cells at a relativelyearly age can make many people worry

that normal lapses in memory, which we all have, are early signs of a devastating dementing illness.”

But Dr. Carlson, one of the country’s leading Alzheimer’sresearchers, also is encouraged by a new coordinatednational effort to attack these diseases before theyravage the brain. The urgent problem of an impendingepidemic of Alzheimer’s disease has prompted the U.S. government to enact a plan to prevent and effectively treat it by 2025.

Finding genetic indicators or markers for Alzheimer’s isone of the plan’s priorities, so that the disease can bedetected and either slowed or stopped in patients beforeit harms them. Researchers have identified severalmarkers for Alzheimer’s, but getting access to them inpatients involves invasive or very expensive procedures.Given these difficulties, people are tested for thesemarkers only after they have developed symptoms.

A simple blood test as a method for detecting diseasemarkers could make very early diagnosis possible, before full-blown symptoms appear. The team of scientists at MRI and their collaborators have beenlooking for markers for various brain diseases forsome time and have helped advance the movementtoward a blood test as a method for early diagnosis.(Early diagnosis is a key objective of the national planto combat the disease.) In an exciting developmentthis past year, the Carlson lab at MRI, David Westway’sgroup at the University of Alberta and their collaboratorswere successful in identifying a marker specific to priondisease, a related class of degenerative brain disease.

Now the MRI group and Dr. Lee Hood’s team at theInstitute for Systems Biology are applying a similarapproach to the search for a genetic marker specific to Alzheimer’s. Since mice are useful only toa certain degree in this particular search, human cellsneed to be a key component. This study could begreatly enhanced by a developing partnership betweenMRI and Benefis Health System, in which cells frompatients could be used to help identify the pre-clinicalmarkers for the disease.

The possibilities for advancing Alzheimer’s researchwith the use of cells from human patients include making new nervous system stem cells from a patient’sown cells and reintroducing them into the patient to replace diseased cells with healthy ones or to deliverbeneficial molecules. Such a dramatic therapy wouldtake years to develop. In the meantime, transplanting

MRI’s Progress In Sync with “National Plan to Address Alzheimer’s Disease”

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Teamwork McLaughlin scientists use varied approaches to unlock the mysteries of Alzheimer’s. Pictured from top: Drs.Teresa Gunn, Brenda Canine (with student Kelsey Smith), and Dan Zou; research assistant Derek Silvius.

stem cells from patients with Alzheimer’s or other braindiseases into mice could help develop a new way to studythe disease in human cells integrating into a living brain.

This enhanced connection between basic Alzheimer’sresearch and patient care, known as translational research, isanotheroneoftheNationalPlan’spriorities.Speeding up the process of applying research resultsto medicine will make a difference for the millions ofAmericans facing the impact of Alzheimer’s diseaseon themselves and their families.

The statistics are growing to encompass most Americansas eventually either patients or caregivers of a spouseor parent with dementia. According to the NationalPlan to Address Alzheimer’s Disease, “Given the greatdemographic shifts that will occur over the next 30 years,including the doubling of the population of older adults,the success of this effort is of great importance to peoplewith AD and their family members, public policy makers,and health and social service providers.”

MRI is poised to take this important next step in thefight against Alzheimer’s and other degenerative braindiseases as part of an urgent push on the part of theUnited States to address a costly health crisis.

The staggering yearly cost of caring forthe entire nation’s Alzheimer’s patients isprojected to be $1.1 trillion by 2050.

Funding Challenges forAlzheimer’s Research

To launch its NationalPlantoAddressAlzheimer’sDisease, the Obama administration has provided some interimfunding for Alzheimer’s research. Full funding for theplan, and for biomedical research in general, will dependupon the outcome of legislative budget debates.

Congress determines the budget for the National Institutes of Health (NIH), which provides the bulk offederal funding for medical research. When adjusted for inflation, purchasing power of this research fundinghas declined over the past decade, making it impossiblefor scientists to carry out the same scope of work.

With this as a starting point, further cuts could be devastating for Alzheimer’s research.

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Supporters Dr. Paul Melvin and his wife Terry at home in Helena, Montana.

Learning to Cope with Parkinson’s Disease

When Dr. Paul Melvin retired from a successfulcareer as a surgeon with the Great Falls OrthopedicAssociates and later moved to Helena, he lookedforward to indulging his love of hiking. He and hiswife, the artist Terry Melvin, built their dream house on thelower slope of Mount Helena so they could spend theirdays hiking from their back door. But after several years,Dr. Melvin wasn’t keeping up during their hikes and beganhaving trouble getting out of his car. He had extreme fatigue and had lost his sense of smell.

Eventually a neurologist diagnosed Parkinson’s disease,and the Melvins’ life became something different fromwhat they’d hoped for. Paul had to give up his work atthe VA orthopedic clinic. A skiing accident last yearmarked the end of his skiing days, and biking nolonger works, either. He has difficulty dressing himself–buttons are a particular challenge, and his voice hasweakened considerably.

Parkinson’s is a degenerative brain disease thataffects more than a million Americans. The braincells that produce dopamine die, making it difficult fornerves to send messages. Muscle movement no longerfunctions properly, resulting in stiffness or rigidity,slowness of movement, tremors and impaired balanceand coordination. Other symptoms can include problems with memory and other cognitive trouble, altered speech, insomnia and depression.

“I wasn’t sure I could handle this,” Paul said of hisearly reaction to the diagnosis. Terry, too, has had atough time adjusting to the illness. “It has been very difficult to learn to cope with what’s happened and to

realize this is how life is going to be,” she said. She went through a dry spell with her art, where she felt“locked up,” and they both had to guard against atendency to withdraw socially as they have adjustedto their new circumstances. According to Paul, “People with these diseases need to realize it has a profound effect on your loved ones.”

Dr. Melvin manages his symptoms fairly well so farwith medication and exercise. “But I recognize it’s aprogressive disease,” he said. Treatments alleviatesymptoms but do not slow the disease’s progression.After Paul began taking medication, he had awful anxiety attacks. “I’d always thought of anxiety assomething you could control, but I had no control overmy racing mind and I couldn’t sleep.” An anti-anxietymedication has made a big difference in his ability to copewith the disease rather than succumb to depression.Having a good doctor and support from Terry havealso helped. “She has been absolutely marvelous,” he said. The Melvins celebrated their 50th weddinganniversary in August.

As a physician, and as a member of a family with ahistory of neurological diseases – his mother hadAlzheimer’s and his father had ALS (also known asLou Gehrig’s disease) – Dr. Melvin knows the vital roleresearch plays in treating neurological diseases.“Basic research is the only way you have of findingcures and the pathogenesis of the disease. Youcan do all these clinical studies, but they don’tusually find the cause of problems.”

Scientists at MRI are working to better understand different aspects of Parkinson’s disease.

Assistant Professor Deborah Cabin’s mouse models for Parkinson’s allow her tosee what happens in the early stages of the disease, in the brain stem, before itmoves to the midbrain and affects movement and balance. “Our hope is to learn

what could stop the progression of Parkinson’s in its early stages,” she said.

Now that scientists know that Parkinson’s, Alzheimer’s, prion and other neurodegenerative diseases share a basic mechanism of misshapen proteins

spreading through the brain cell by cell in a cascade of dying neurons, MRI’s workto learn more about the specific mechanism for each of these disorders is

strengthened by the Institute’s team approach to this class of diseases. According to Dr. Cabin, “It’s an exciting time to be studying Parkinson’s.”

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MRI’s Model Science Educat ion Program Has Far-Reaching Impact

In the lab Teacher cohort members Tom Cubbage, Dan Rediske and Rob Truax discusscurriculum improvements for the upcoming school year.

Tom Cubbage is thrilled that a number of his formerscience students at C.M. Russell High School inGreat Falls are now working as researchers in labsacross the country. Three are currently working as research assistants or postdoctoral fellows atMcLaughlin Research Institute, where Mr. Cubbagehas spent nine summers working in the educationprogram. He attributes the pursuit of science careersby his students to the MRI program, in which teachersand students work side by side with researchers in thelab. The students get a chance to find out whetherscience is their calling, and the teachers learn “realscience” techniques they can use back in class.

“Because MRI is in Great Falls,” Mr. Cubbage says,“rural Montana students believe research as a career is a possibility. [The program has expandedto include teachers from rural communities.] Ifyour science teacher can do it, you realize it’s anoption.” He knows the enthusiasm he brings back tothe classroom after spending a summer immersed inthe intellectually stimulating atmosphere of science is contagious. “I get such rejuvenation for teaching; it gets me excited about the science every year. I love sharing that with the kids and talking aboutthe high-level new things going on in science,which really wows them,” he said.

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Collaboration and learning In one of the mouse housing areas, teacher Kiran Satyavarapu makes notations while working alongsidestudents Jourdon Gudatis and Kaitlyn Carlson. Teachers in the 2012 program not pictured: Allyson Hoof and Rachael Newmiller.

“The impact of this kind of program is so far-reachingthat we have no idea of the extent of this. Each one ofus teachers is impacting 90-120 students every yearfor the rest of our careers. Every one of our studentsgets exposed to this excitement about science.”

The involvement of teachers at MRI increased dramaticallyfive years ago when the Howard Hughes Medical Institute(HHMI) began funding a new program that has allowedteachers to spend two summers at McLaughlin. Theyspend one summer in the lab and the second summerworking with a small team of teachers to integrate their research experience into the curriculum.

In addition to the various opportunities MRI has offeredteachers for nearly 20 years, the Institute has been trainingstudent interns for more than 50 years, since renownedStanford University stem cell expert Irving Weissman’s initiative as a high school student prompted Dr. ErnstEichwald to let him work in the lab. Over the years, theprogram has grown into one of the best in the countryand has become quite competitive.

Tom Cubbage studied other programs and was notsurprised to learn that “MRI has most places beat,” interms of providing opportunities for serious immersionin actual science. He said it’s rare that students get to doa project from start to finish, and even more rare to haveteams including a teacher, a high school student and a college student in one lab. “No other program does that,”according to Mr. Cubbage.

Great Falls Public Schools appreciates the program theirteachers and students participate in. According to TomMoore, Assistant Superintendent for Secondary Schools,“This is a model program, providing meaningful, relevant preparation for careers. Teachers and studentsget to work to help solve a big, important problem ingenetic research and realize they could make a difference some day. We’re so fortunate in this community to have this opportunity with MRI for students and teachers to work side by side with scientists.”

Teacher Immersion The M.J. Murdock Charitable Trust’s Partners in Science program also has provided funding for individualteachers in the past, including Tom Cubbage (facing page far left).

This year Nathan Gregier (top), his colleague at C.M. Russell HighSchool, received the grant, while Mr. Cubbage assumed the role ofcircuit rider, visiting all Partners in Science teachers in Montana.

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Students Get Serious About ScienceEach summer, the labs at MRI are filled with high school and college students, learning the ropes of real science. As Great Falls Public Schools’ Tom Moore said after watching the 2012 students present their research results, “Students get to work to help solve a big, important problem in genetic research and realize they could make a difference some day.”

Summer students Left to right, from top: Garret Morrill, Jourdon Gudatis, Kaitlyn Carlson, Lauren Smith, Alekses Clifton, Emilie Jacobsen and Raphael Broh. Not pictured: Kelsey Jo Smith and Jojo Coburn.

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Novel approach Dr. Orr has published work on stem cell models for dementing disease and now worksin San Antonio, Texas, where she is respected for her knowledge of mouse genetics.

As a graduate student who spent several yearsconducting doctoral research at McLaughlin Research Institute, Miranda Yeska-Orr developeda novel stem cell model for frontotemporal dementia, which is closely related to Alzheimer’sdisease. Her work was published in the June 2012issue of the peer-reviewed journal PLOS ONE.

“We were able to see pre-pathological phenomenathat could be further studied to determine whetherthey will serve as useful markers for the disease,” she explained. Finding markers for degenerative brain disease is one of the goals for both MRI and the nation (see article on page 4), since they could be useful in very early diagnosis.

Dr. Yeska-Orr is continuing her research in her newpostdoctoral position at the Barshop Institute forLongevity and Aging Studies in San Antonio, Texas.She is working to improve current mouse models forAlzheimer’s and is using a line of genetically engineeredmice unique to McLaughlin, which has supplied herwith mice to start her own colony. “I’m amazed,when I look back, at how much I learned at MRI,”she said. “The people in the lab here now rely onme for my knowledge of mouse genetics.”

Miranda grew up in Saco, Montana, where her workon the family ranch influenced her interest in science.Her grandmother’s death from Alzheimer’s inspired herto study dementing diseases, which she began studyingin George Carlson’s lab at MRI in 2007. She receivedher PhD from Montana State University in 2011.

“I’m convinced that Miranda will have a brilliantcareer in research and make significant contributionstowards treating degenerative brain diseases,”Dr. Carlson said.

According to Dr. Yeska-Orr, “Being a native Montanan,I always heard that there are bigger, better things outthere, and that we’re disadvantaged somehow inMontana. I’m sure that’s not true. The science goingon at MRI is top notch, and I was lucky to do my research there.”

“Dr. Carlson really fosters independent thought,and I think that’s the most important thing gradstudents learn: how to think critically and how todesign experiments. You can’t go to a textbook oronline and learn those things.” Miranda went on tosay that without those skills, it also would be difficultto write grants, and that she learned at MRI how towrite a successful grant and how vital grants are tothe work of a scientist. “I was able to get my currentjob because I had recently received my own fundingby applying for a grant. I can’t say how much myexperience at McLaughlin has meant to me.”

FormerMRIGradStudentAdvances Alzheimer’s Research

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George Carlson, PhD

July 2011 Presentation, Early Detection & Prevention of Alzheimer's Disease & Other Dementing Disorders,at the Champions of Quality Conference, Montana Hospital Association, Helena, MT.

August 2011 Presentation, Transgenic Models for Frontotemporal Dementia: Role of Endogenous Mouse Tau,at McLaughlin Research Institute’s Annual Biomedical Sciences Workshop, Great Falls, MT.

October 2011 Board of Director’s Meeting, N. Bud Grossman Center for Memory Research and Care, at the University of Minnesota, Minneapolis, MN.

Presentation, Research at McLaughlin Research Institute, at the Cascade County Bar Association Meeting, Great Falls, MT.

November 2011 Department of Biological Sciences Advisory Board Meeting, at Montana Tech, Butte, MT.

December 2011 Consulted for StemCells Inc. on their Disease Team grant application entitled Restoration of memory in Alzheimer’s disease: a new paradigm using neural stem cell therapy, which was submitted to the California Institute of Regenerative Medicine, the meeting was held at University of California, Irvine, CA.

March 2012 Testified on the importance of NIH funding, before the Labor, Health and Human Services, Education, and Related Agencies Subcommittee of the House of Representatives, Washington, DC.

April 2012 Attended the Opportunities for business and education cooperation with the Montana LearningCenter, Reception hosted by Governor Schweitzer and the First Lady, Helena, MT.

May 2012 Presentation, Expression of wild type tau may delay or decrease pathology in the rTg (tauP301L ) model for frontotemporal dementia, at the Prion Review Meeting,San Francisco, CA.

May 2012 Presentation, Expression of wild type tau may delay or decrease pathology in the rTg (tauP301L ) model for frontotemporal dementia, at the Montana Neuroscience Retreat, Bozeman, MT.

John Bermingham Jr, PhD

March 2011 Poster Presentation, Translational control of expression of the epilepsy-related protein Lgi1.at the American Society for Neurochemistry conference, St. Louis, MO.

October 2011 Presentation, The secreted protein Lgi4 is required for peripheral nerve developmentat Biogen, Cambridge, MA.

Deborah Cabin, PhD

August 2011 Presentation, Genetic approaches to alpha-synuclein function and toxicity, at the McLaughlin Research Institute Biomedical Sciences Workshop, Great Falls, MT.

September 2011 Presentation, Research overview of the McLaughlin Research Institute, at the Optimists Club meeting, Great Falls, MT.

October 2011 Presentation, Can mouse alpha-synuclein help us understand human alpha-synuclein toxicity?at the Weissman Lab Retreat, Rocky Mountain Laboratory, Hamilton, MT.

January 2012 Presentation, Research overview of the McLaughlin Research Institute and Neurodegenerative Diseases, at the Leadership Great Falls meeting, Great Falls, MT.

Scientists Learn, Share and Contribute

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Teresa Gunn, PhD

October 2011 Presentation, Mitochondria, ubiquitination and neurodegeneration, at theWeissman Lab Retreat, Rocky Mountain Laboratory, Hamilton, MT.

2012 Reviewed a grant application for the National Institute of Health Molecular, Cellular and Developmental Neuroscience study section.

Reviewed a grant application for the Alzheimer’s Association, grant review committee.

John Mercer, PhD

July 2010 –August 2011 Visiting scholar in the Department of Biochemistry, Stanford University, School of Medicine,

Palo Alto, CA.

August 2011 Presentation, A high-throughput approach to inherited cardiomyopathies, at the McLaughlin Research Institute Annual Workshop, Great Falls, MT.

November 2011 Presentation, Genetics of cardiomyopathies, at the Indo-Spanish workshop on health andmedical research, New Delhi, India.

December 2011 Poster presentation, Building an international network for student training and research collaboration in the biological sciences, at the International Institute for Collaborative Cell Biology and Biochemistry.

Poster presentation, A subset of axonally transported RNAs associates with Myosin-Va in ribonucleoprotein complexes and Schwann cells as local supply of axonal RNA in regeneratingmammalian nerves, at the American Society for Cell Biology annual meeting, Denver, CO.

February 2012 Presentation, A high-throughput approach to inherited cardiomyopathies, at theSecond Indian Ocean Rim Muscle Colloquium, Bangalore, India.

April 2012 Attended the Scientific innovations in pediatric and congenital heart disease meeting at Stanford University, Palo Alto, CA.

Brenda Canine, PhD

July 2011 Attended the Systems Biology Course, at the Institute for Systems Biology, Seattle, WA.

October 2011 Presentation, Utilization of systems biology approaches to identify candidate genes affectingscrapie prion incubation time, at the Weissman Retreat, Hamilton, MT.

Miranda Orr, PhD

December 2011 Dissertation seminar and defense, Mouse and Stem Cell Models of Frontotemporal Dementia.at the Department of Cell Biology and Neuroscience, Montana State University, Bozeman, MT.

Will Walker, PhD

October 2011 Presentation, Tsg101 depletion causes neurodegeneration and spongiform encephalopathy,at the Weissman Retreat, Hamilton, MT.

Dan Zou, PhD

August 2011 Presentation, How does mouse alpha-synuclein protect against human A53T mutant humanalpha-synuclein toxicity? at the McLaughlin Research Institute Biomedical Sciences Workshop,Great Falls, MT.

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The list above includes articles published July 1, 2011 - June 30, 2012.

Scientific PublicationsThe scientists at McLaughlin collaborate with colleagues worldwide to advance knowledge by publishing articlesin scholarly peer-reviewed journals.

Fox LM, William CM, Adamowicz DH, Pitstick R,Carlson GA, Spires-Jones TL, Hyman BT. Soluble tau species, not neurofibrillary aggregates, disrupt neural system integration in a tau transgenic model.Journal of Neuropathology & Experimental Neurology.2011 Jul; 70(7):588-95.

Kopeikina KJ, Carlson GA, Pitstick R, Ludvigson AE,Peters A, Luebke JI, Koffie RM, Frosch MP, Hyman BT,Spires-Jones TL. Tau accumulation causes mitochondrialdistribution deficits in neurons in a mouse model oftauopathy and in human Alzheimer’s disease brain.American Journal of Pathology. 2011 Oct; 179(4):2071-82.

Westaway D, Genovesi S, Daude N, Brown R, Lau A,Lee I, Mays CE, Coomaraswamy J, Canine B, Pitstick R, Herbst A, Yang J, Ko KW, Schmitt-Ulms G,Dearmond SJ, McKenzie D, Hood L, Carlson GA.Down-regulation of Shadoo in prion infections traces apre-clinical event inversely related to PrP(Sc) accumulation.PLoS Pathogens. 2011 Nov; 7(11):e1002391.

de Calignon A, Polydoro M, Suárez-Calvet M, William C,Adamowicz DH, Kopeikina KJ, Pitstick R, Sahara N,Ashe KH, Carlson GA, Spires-Jones TL, Hyman BT.Propagation of tau pathology in a model of earlyAlzheimer’s disease. Neuron. 2012 Feb 23; 73(4):685-97.

Park H, Staehling K, Tsang M, Appleby MW, Brunkow ME,Margineantu D, Hockenbery DM, Habib T, Liggitt HD,Carlson GA, Iritani BM. Disruption of Fnip1 reveals ametabolic checkpoint controlling B lymphocyte development. Immunity. 2012 May 25; 36(5):769-81.

Bajsarowicz K, Ahn M, Ackerman L, Dearmond BN,Carlson GA, DeArmond SJ. A brain aggregate modelgives new insights into the pathobiology and treatmentof prion diseases. Journal of Neuropathology & Experimental Neurology. 2012 May; 71(5):449-66.

Daude N, Wohlgemuth S, Brown R, Pitstick R,Gapeshina H, Yang J, Carlson GA, Westaway D.Knockout of the prion protein (PrP)-like Sprn gene doesnot produce embryonic lethality in combination withPrP(C)-deficiency. Proceedings of the National Academyof Sciences USA. 2012 Jun 5; 109(23):9035-40.

Lan X, Kiyota T, Hanamsagar R, Huang Y, Andrews S,Peng H, Zheng JC, Swindells S, Carlson GA, Ikezu T.The effect of HIV protease inhibitors on amyloid-peptidedegradation and synthesis in human cells and Alzheimer’sdisease animal model. Journal of NeuroImmune Pharmacology. 2012 Jun; 7(2):412-23.

Fanara P, Wong P-Y, Husted KH, Liu S, Liu VM,Kohlstaedt LA, Riiff T, Protasio JC, Boban D, Killion S,Kiooan M, Epling L, Sinclair E, Peterson J, Price RW,Cabin DE, Nussbaum RL, Bruhmann J, Brandt R,Christine CW, Aminoff MJ, Nellerstein MK.Cerebrospinal fluid-based kinetic biomarkers of axonaltransport in monitoring neurodegeneration, Journal ofClinical Investigation. 2012, 122(9): 3159-3169.

Spires-Jones TL, Fox LM, Rozkalne A, Pitstick R,Carlson GA, Kazantsev AG. Inhibition of sirtuin 2 with sulfobenzoic acid derivative AK1 is non-toxic and potentially neuroprotective in a mouse model of frontotemporal dementia. Frontiers in Pharmacology.2012; 3:42.

Orr ME, Pitstick R, Canine B, Ashe KH, Carlson GA.Genotype-specific differences between mouse CNSstem cell lines expressing frontotemporal dementia mutant or wild type human tau. PLoS One.2012;7(6):e39328.

Jesty SA, Jung SW, Cordeiro JM, Gunn TM, Di Diego JM,Hemsley S, Kornreich BG, Antzelevitch C, Moïse NS.Cardiomyocyte calcium cycling in a naturally occurringGerman shepherd dog model of inherited ventricular arrhythmia and sudden cardiac death. Journal of Veterinary Cardiology. In press.

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Seminars by guest scientists enrich the academic environment at McLaughlin Research Institute and are opento Montana’s medical and university communities.

Seminars

The list above includes talks given July 1, 2011 - June 30, 2012.

August 17, 2011Genetics of the normal immune system revealed by the collaborative crossJeff Frelinger, PhDUniversity of ArizonaTucson, AZ

Genetics of color patterns: model systems and model organisms in a post-genome worldGreg Barsh, MD, PhDStanford University,Palo Alto, CA

Harnessing transposons for cancer gene discoveryNancy Jenkins, PhDInstitute of Molecular & Cellular Biology,Singapore

Transposon-based insertional mutagenesis identifiesgenes that transform neural stem cells into glioblastoma tumor-initiating cellsNeal Copeland, PhDInstitute of Molecular & Cellular BiologySingapore

Stem cells gone wildIrv Weissman, MDStanford University,Palo Alto, CA

Traumatic brain injury: environmental initiation of prion-like, tauopathic, neurodegenerationDan Perl, MDUniformed Services University of the Health SciencesBethesda, MD

Systems medicine and P4 medicine—a proactive medicine of the futureLee Hood, MD, PhD Institute for Systems BiologySeattle, WA

August 26, 2011Brain aggregates: a novel and accurate in vivo model of prion diseaseStephen J. DeArmond, MD, PhDInstitute for Neurodegenerative DiseasesSan Francisco, CA

November 16, 2011 HHMI Lectures Endless flies most beautiful: gene co-option and the evolution of animal form

Remarkable creatures: epic adventures in the search for the origin of speciesSean Carroll, PhDHoward Hughes Medical InstituteChevy Chase, MD University of WisconsinMadison, WI

March 21, 2012Utilization of mouse fetal brain derived neurosphere culture as a novel in vitro model of Alzheimer’ s DiseaseRanjit K. Giri, PhDNational Brain Research CentreManesar, Haryana, India

May 9, 2012A global consortium to study inherited cardiomyopathiesJohn Mercer, PhDInstitute for Stem Cell Biology and Regenerative Medicine (inStem)National Centre for Biological SciencesBangalore, India

16

Scientific Advisory Committeemembers convene in Great Falls

Irv Weissman, Neal Copeland, David Cameron,Nancy Jenkins, Jeffrey Frelinger, and Leroy Hood.

Not pictured: David Baltimore.

StaffDirector & ProfessorGeorge A Carlson, PhD

Professor John A Mercer, PhD

Associate ProfessorJohn R Bermingham Jr, PhD

Associate Professor &Transgenic Facility AdvisorTeresa M Gunn, PhD

Assistant ProfessorDeborah E Cabin, PhD

Postdoctoral FellowsBrenda Canine, PhDAndrea Grindeland, DVMMiranda Orr, PhDWill Walker, PhDDan Zou, PhD

Senior Research AssistantsRebecca BrownJill O’MooreRose Pitstick

Research AssistantsSarah AndersonMegAnne CaseyDelisha MeisherySydni RackiDerek SilviusKaty K Walker

New Lab Complex Scientific Coordinator & Mouse Management LiaisonInstitute for Stem Cell Biology and Regenerative Medicine,National Centre for Biological SciencesBangalore, India

Colleen Silan

Transgenic AssistantSherry Turner

Research TechnicianJanet Peters, LATG

Animal Resource SupervisorJulie Amato, LAT

Animal Care TechniciansMallory BrumleyKelli JohnsonJennifer LawrenceRachel MardenAnita Pecukonis, RLAT

Animal CaretakerJonathan Woods

Cage Wash TechniciansJoseph GilmoreBrandon Moe

Glassware TechnicianMelany Gilmore

Chief Advancement OfficerBarbara Ball-McClure

Development OfficerLisa B Flowers, PhD

Administrative AssistantsRandi Graves, MASylvia Love, MA

Assistant to the DirectorJill O’Moore

Financial OfficerColeen Balzarini, MAS, CPFO

Purchasing AgentWanda Forster, CPP

MaintenanceJoe Amato, LAT, SupervisorMelany Gilmore

List includes staff with us betweenJuly 1, 2011 and June 30, 2012.

17

Scientific Advisory Committee

Chair, Irving L Weissman, MDDirector, Institute for Stem Cell Biologyand Regenerative MedicineStanford University School of MedicinePalo Alto, CA

David Baltimore, PhDCalifornia Institute of TechnologyPasadena, CA

David Cameron, PhDProfessor Emeritus Montana State UniversityBozeman, MT

Neal G Copeland, PhDThe Methodist Hospital Research CenterHouston, TX

Jeffrey A Frelinger, PhD University of ArizonaCollege of MedicineTucson, AZ

Leroy E Hood, MD, PhD Institute for Systems BiologySeattle, WA

Nancy A Jenkins, PhD The Methodist Hospital Research CenterHouston, TX

Board of Directors

Chair, Kathy Rice

Vice-Chair, Bethann McGregor

Secretary, Brad Talcott

Treasurer, John Lawton

Brice Addison, MD

Shannon Anderson

Gary Bjelland

David Cameron, PhD

George A Carlson, PhD

Nancy Davidson

Randy Gray

Susan Humble

Phillip A Krezowski, MD

John Lane, Cascade, MT

Jann Leppien, PhD

Leslie Oakland

Sandy Peters

Evelyn Rider, MD

Erik Sletten

Irving L Weissman, MDPalo Alto, CA

Honorary TrusteeArlyne Reichert

Board members reside in Great Falls, Montana unless noted otherwise.

National Development Council

Chair, Nancy Davidson, Great Falls, MT

Ardi Aiken, Bigfork, MT

Gary Buchanan, Billings, MT

David Cameron, PhD, Great Falls, MT

Nick Cladis, Billings, MT

Gerald Davidson, Red Lodge, MT

Brett Doney, Great Falls, MT

Paul Eichwald, Missoula, MT

Becky Fonda, Livingston, MT

Jon M Huntsman, Salt Lake City, UT

Robert F Jorgensen, Great Falls, MT

Richard E Lauritzen, MD, Great Falls, MT

Bill Macfadden, Great Falls, MT

Matt Mallow, New York, NY

Sally McGregor, Great Falls, MT

Leslie Oakland, Great Falls, MT

Nancy O’Brien, Great Falls, MT

Sandy Peters, Great Falls, MT

Cindy Poett, Ovando, MT

Arlyne Reichert, Great Falls, MT

Gina Reilly, Helena, MT

Montana First Lady Nancy Schweitzer Helena, MT

Robert Sletten, Great Falls, MT

Nina Smith, Helena, MT

Nancy Souder, Bigfork, MT

Jim Strauss, Great Falls, MT

Gorham E Swanberg, Great Falls, MT

Eugene B Thayer, Great Falls, MT

Mary Edith Thogersen, Tucson, AZ

Irving L Weissman, MD, Palo Alto, CA

NDC Members At Large

US Senator Max Baucus, Washington, DC

Montana Rep. Mike Milburn, Cascade, MT

US Rep. Dennis Rehberg, Washington, DC

US Senator Jon Tester, Washington, DC

Montana Senator Mitch Tropila, Great Falls, MT

Partners in Research

18

You can help by supporting our research now … so we can help patients in the future.

Generous gifts made to McLaughlin Research Institute help fuel innovative, collaborative, and community-based research and education. Your meaningfulcontributions go directly to the research youwant to support. Together with our collaborators (LeeHood, Brad Hyman, and Irv Weissman among them),we are building an unparalleled understanding of theprocesses causing degenerative brain diseases.

This year the Institute has been blessed with theMazurek family’s willingness to help play a role inthe search for a cure to Alzheimer’s disease. Thegifts in honor and memorial of Joe are true testimonialsby the many individuals who were touched by hisfriendship, leadership and commitment to his family.The Institute is honored to work with Randy Gray, current board member, and the “Friends of Joe” togrow financial support for Alzheimer’s research now, and to help patients in the future.

There are many meaningfulways to give to McLaughlin Research Institute. We hope youwill choose one that is best suitedto your needs and interests.

It would be a pleasure to help guide your philanthropy.Please contact our development officer, Dr. Lisa Flowers,at 406.454.6009.

The McLaughlin Research Institute has been awarded the

highest “four stars out of a possible four” rating for sound

fiscal management and commitment to accountability and

transparency by Charity Navigator, the country’s largest and

most-utilized independent evaluator of charities. Only17percent

of charities rated have received at least two consecutive

four-star evaluations, indicating that MRI

in Great Falls, Montana outperforms

most other charities in America.

Troy Florence Wainscoat, a gracious patron of McLaughlin Research Institute,recently died of natural causes in SouthLake Tahoe, California. Troy lived in SantaCruz for many years prior to living in Great Fallsfor the past three years. She was born in SanFrancisco and lived in many areas of Californiaover her 93 years.

Troy was married to Conrad Wainscoat and had 16children, 47 grandchildren and many great-grandchildren.

“Troy loved biomedical research and was very interested in it,” said longtime friend Betty VanLieshout. She supported other healthcare organizationsincluding the AIDs Foundation and St. Jude Children’sResearch Hospital.

For a while, she lived down the street from the Institutein the Renaissance Senior Care facility. Troy and Conraddonated to MRI over time. “It always made her feel good,

when she looked across the street, to know that she had contributed in a small way to Alzheimer’s research,”Betty recalled.

Troy’s family remembers her as afriend to all and an example of true

unconditional motherly love. She was a musician andavid reader, loved to learn about the world and traveled to Europe with Conrad.

At age 85, she traveled to South Africa with her trustedfriend, Betty. She had many great adventures and herfamily loved to hear her stories. She loved Montanaand before moving here she visited every year so shecould enjoy the “Big Sky,” mountains and lakes.

Troy was thankful for all the care and friendship theGreat Falls community gave to her, and her gift of a$25,000 annuity to McLaughlin Research Institute wasa generous way for her to give back to Great Falls.

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COMPANY EMPLOYEE MATCHING GIFTSClorox Company FoundationDavidson CompaniesPrudential Foundation Matching Gifts

IN KINDFran CarlsonNeal Copeland, PhDJanet HendersonNancy Jenkins, PhDPam Lemelin, Renaissance Creative

Our Donors GiftsreceivedJuly1,2011throughJune30,2012. Please check these lists for accuracy. You deserve proper recognition for your gifts and, while wemake every effort to get the names correct, occasional errors occur. If you find we omitted or misspelled your name or placed you in the wrong givingcategory, please call the Development Office at 406.454.6009 to allow us to apologize and correct the records. We want to correct even “minor” errors. Thank you again for your gift and, if necessary, your forbearance.

Gifts like these help make it possible for the Institute to carry out its important mission ofimproving human health through research and education.

LEADERIan & Nancy Davidson

COLLABORATORLeslie Oakland

FOUNDERTheCharlesEngelhardFoundationRobert & Shirley JorgensenSally T. McGregorGene & Jane Thayer

BENEFACTORAnonymousD. A. Davidson & Co.Davidson Family FoundationFraternal Order of Eagles

(Grand Aerie)R. & F. Jorgensen FoundationDr. & Mrs. Philip KrezowskiRobert & Pat Sletten

PATRONAnonymous Private FoundationDrs. Andreas Luder &

Deborah ErdmanNancy & Buck O’BrienEstate of Evelyn E. PonceletLola PygottUS Bank

Chris Reiquam & Kevin Clark

ASSOCIATEBob & Jane BeckerFern BlewettDr. & Mrs. J. W. BloemendaalPeggy Lee BlythJack & Mary DykstraRandy & Nora Flaherty GrayDr. Charles & Gerry JenningsRoland & Helen Lapee

Family FoundationDr. & Mrs. R. E. LauritzenMary K. McGregor &

Eric SchultzStuart & Jane NicholsonCindy & Harry PoettErik SlettenLeroy StrandBradley Talcott &

Linda CaricaburuDuane Talcott

FRIENDAnonymousRoy & Donna AafedtHussain Abdul-HussainCheryl & Doug AkelstadThe Albrecht Family TrustDr. F. John & Robin AllaireCurt & Betty AmmondsonWarren N. AndersonJames & Carol AuthDrs. John & Susan AveryAudy & Helen BaackLucille BalfourColeen & Tom BalzariniMr. & Mrs. Aldo BarbagliaBarrick Electric

Leo & Marilyn BarrickRichard & Rayleen BeatonIsabel BeermanMarge & Douglas BensleyKatherine McGee BenzelBermingham Fund

John R. BerminghamHoward BethelNiki & Donald BickelBig Sky Oncology

Dr. & Mrs. Grant HarrerJudith BirchGary & Kay BjellandNorris & Doris BjorkFrancis & Sandra BlakeJohn R. BlanchetJanice M. BlomJohn BohlingerJohn & Betty BorchersLillian BorgeW. L. & Jackie BourretRobert BowmanMarie BoydDoris & Joseph BoyleCarrie BoysunJeanette BridgefordH. P. BrownPeggy Browning

Bret & Susan BrunnerOliver & Betty BucklinBrian BurdSean & Patricia BurkeDr. Peter & Irene BurleighLorraine BurrisClyde & Carol ByerlyDeborah CabinJohn H. & Elizabeth CaldwellClifford & Walline CampbellAlbert & Betty Carleton Dan & Madeline CarsonCascade Electric Co.

Scott & Sandi WilsonMary Casey & Mike DavisDr. Robert & Thelma CaseyNick & Patricia CettoMr. & Mrs. Jim ChristensenChurch Harris Johnson

& WilliamsHelen ClaryJean ClaryRichard A. ClaryRobert F. ClaryClorox Co. FoundationJohn & Patty CohlheppJay & Sheila ConlonBruce ConnollyNeal Copeland, PhDHelen CoryGlenn & Leanna CoulterRalph Cox &

Alta Mae Buford-CoxPeggy Louise CrawfordWilliam & Carol CrewsKevin CroffDr. Valerie DanielsonAndrew & Wendy DavidsonKaren DavidsonGary & Kathleen DavisJack R. DavisMargaret DavisAnn & Stephen DayMike & Diana Delesha

Lifelong Great Falls resident Evelyn Poncelet was impressed bywhat she learned about MRI’s workto help find cures for deadly diseases.She included a bequest for the Institute in her will, and after her death in December 2011 at age92, MRI received a $40,000 gift from her estate.

Evelyn and her late husband, Nicholas, ownedand operated Poncelet Landscaping until herretirement in 1972. The couple had three sonsand numerous grandchildren, great-grandchildrenand great-great-grandchildren. Evelyn was an

antique car buff and a member of the Great FallsFlower Growers.

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FRIEND

Rich Deming & Julie BennettRichard & Donna DengelMarilyn DeZortRobert & Marlene DillardThe DILsTamara DimkeAgnes DorsWarren & Nancy DrazichKenneth DuncanAnna DwyerAnnie DyerSheila EastmanHelen Easton Tom & Marcia EckersonShirley EdwinJane EhlangSissy EichwaldDr. James & Gayle EidsonMark & Sue ElingsJohn D. & Mary EnglandCarl & Anna May EricksonRoger & Jerry EvansKim & Linda FagenstromDenise FellerAnna Mae FlemingDonna FornfeistDonald & Michele FoxJohn & Aggie FrankinoSteve & Linda FranklinSheryl FridenFriends of Alice Tatchell

LSU HSC Dept. of Biochemistry

Angus FultonRoberta GallagherVincent & Gladys GalliRobbie GammackDr. Gerald Geiszler &

Kathleen BelterDr. George & Diane GelernterHenry GoldhahnNoella GoodellSidney GoodrichAnn Clary GordonKarla Gray & Myron Currie, Jr.Robert & Donna GreytakRichard GriebThomas & Barbara GrimesNorma HabelMarianne HaighOpal HallaCarl & Pearl HansenMargaret HardenNicholas & Evette HauerRobert & LeeAnn HawkinsShane HedgesRose HedrickJames & Deborah Arndt HeimMarty & Gretchen HellerJanet HendersonDr. James Hinde &

Lynn O’Malley, PhDHarold HinmanJames & Cheryl HiroseR. & Karen HodgeKen HolmDonald & Evelyn HoltRobert & Lois HolterMark HomBetty Hotaling

Marian HotvedtBarbara HovenSean & Andrea HovenBernie & Kathy HubleyShirley HudsonDouglas & Barbara HugginsGordon & Judy HuntJohn & Rosemary HusbyPhilip & Sherry IsherwoodBen & Pat IvesAmy R. JacobBetsy JacobShirley J. JacobsenJames & Sharon JacobsonLinda JacquezMark JagelsTed JamesRonald & Jean JarrellNancy Jenkins, PhDMr. & Mrs. Gary JensenMark & Carrie JensenCatherine M. JohnsonCharles Johnson & Pat HuntMike JohnsonPatricia Bushly JohnsonVelma JohnsonJanella JonesJeff & Leeann JovickMarie KallDavid & Diane KathmanIke & Mary Ann KaufmanJoseph D. Keenan IIIRobert & Susan KellerTed & Norma KellyKeith Kemble & Dan FlanaganBarbara KennyTillie KilbyJudy KinonenMr. & Mrs. Richard KnappGene & Bonnie KnightSusie KnightCharlene KommersDarrell & Sharon KorinMaurine KornfeldConnie LaabsJohn & Kendra LaneFloy LarsenJames & Mary LarsonCarole LaValleyDr. Morgan Leach &

Dr. Mindy SternerRichard H. LeeBeverly A. Le MieuxJann Leppien, PhD &

Ed LeppienNancy & Carl LesmeisterRosemary ListonDonald LetzJack & Donna LewisDr. Henry LorentzRay & Lynn LouthenCarol Sue LukesJustin, Nickie, Jackson &

Grace MailletLarry & Delta MailletJamie ManwaringRichard & Carol MarbergMr. & Mrs. Bill MartinMike & Fran MastersRobert & Deloris Mau

Marian MayJohn & Marcia MaynardLorna Mayo Family FoundationJoe & Patricia MazurekW. J. & M. J. McCallMerdyce McClaranJerome & Cecilia McConnellR. W. & Kathryn McDonaldEva McDunnMike & Joy McGrathThomas E. & Barbara McGrathDr. & Mrs. W. R. McGregorFather Francis L. McInnisDorothy Jean McKayRichard & Karen McLaughlinCharles & Carol McWilliamsRobert & Sherry MeadorsBruce & Debbie MedvecPerry & Diane MerkelRoy & Marian MeyerMGM Property Management

Specialists, LLCLouie & Christine Maillet

Brent & Kelly MichelDr. William & Joyce MillerDeLores MillsMitchell Development &

Investments, LLCMr. & Mrs. Ted Mitchell

Harry & Kay MitchellMary & Duane MoeHartwig Moeller &

Janice Moeller-BriggsDennis & Mary MollanderLee & Terry MongeonJayne & Maxwell MooreJeanne MooreDaniel T. MorganJeanine Morreim &

John E. StevensBonnie MosbruckerKenneth & Faye MossRichard & Molly MunroEvelyn MunsonNazdarRobert & Marsha NebelDiane NelsonLois & Roger NelsonRobert & Betty NelsonH. Dean & Nancy Jean NewhouseColleen NicholsAlan & Nancy NicholsonNorth Country Media Group, Inc.

Sandy PetersAlda NurmiBarb O’ConnellClaudia & Patrick O’ConnellDuElla OlsonRichard & Debbie OlsonJim & Anita OppedahlHarlan & Patricia OrhamCatherine M. OrtweinCynthia OverturfGeorge PamenterBonnie PattersonPayne Financial Group, Inc.

Cara PicconoLoretta & Robert PearceBill H. PearsonSpencer Pearson

Randy & Connie PeckJohn & Loretta PejkoDr.John & Betty PfaffRell & Diana PorterFred & Marge PrebleSheryl PresslerPrudential Foundation

Matching GiftsRobert & Charlotte RahnFrank & Erica RamosWilliam & Nancy RamseyMr. & Mrs. Jim RaphaelArlyne ReichertDrs. Roger & Valerie ReichertElizabeth ReidChris & Cindy ReiquamPeter & Sylvia ReissRenaissance Creative

Pam LemelinLoren & Elaine RicePatrick RiceFrank & Grace RichterDr. Evelyn & Gregory RiderKathryn J. Rinehart

Testamentary TrustJeffrey RivardDave & Joyce RobertsIvy RobertsonRockwell CollinsRon Hall Sprinklers

Ron HallEsther RoofJoel RothDr. Stephen RothRouns Land & Livestock, Inc.

Joel & Audrey RounsEmile & Letitia RuffierBetsy Rushworth, PhDJeanne & Chuck SchabelCharles & Diana SchedelSteve SchollElaine Schoyen &

Michael CroskreyAlan & Joyce SchubertDick & Mary SchulerSchwab Charitable Fund

Cindy & Harry PoettLeah SchwandtBrian & Nancy SchweitzerDavid & Shirley ShaneAlfred & Sally ShawScott & Lisa ShullLaurie SizemoreMichael L. SmithSandra SmithRich SohaBetty SolteszRoy & Nancy SouderHelen SpencerEdward & Susan StandleyDr. Catherine SteeleSteinmuller Family

Charitable FundDr. David & Patti Steinmuller

Stella P. Holt FoundationMarlyn McGrath Lewis

Stockmens BankMarilyn Moore

Richard & Carolyn StremchaRichard & Pam Stuckey

21

FRIEND

Honor GiftsAn honor gift supports McLaughlin’s research in the name of a living person.

Super Enterprises, Inc.Mr. & Mrs. James Super

Jerry & Martha SwansonDiana TalcottGarth & Shirley TamsDennis & Joan TaylorJuanita TaylorWyman & Dee TaylorTest Support Group (Medical)

Neutral Buoyancy Lab, NASA

Scott ThackerayDebra Thatcher &

Dr. Nicholas BonfilioRod & Marilyn ThorneBert & Myrna Thurber

Titcomb FoundationJohn & Marcela TomlinHarry TorgersonDr. Gerald & Sharon TuckBarbara TuckerEdwin & Faye TuckerPhyllis A. TurnerNancy & Bob UnferthDavid & Margie UptonBarbara UrquhartJohn & Sandi VanniPennie & Ron VihinenBob WagnerVernon & JoAnne WaldenbergLeland & Margaret WalkerPatrick Ward & Ellen Fineman

Ron & Mignon Waterman Will & Nancy WeaverHarvey & Joan WeikumJerrold & Nadyne WeissmanSue WendeDean & Donna WesterMichael WhalenStephen WhalenRaymond & Kathleen WherleyRichard & Carol WhitmoreBob WightJohn & Beverly WilliamsLarry & Rebecca WilliamsRuth WilliamsDorothy WillitsKevin & Lynda Willms

Dr. & Mrs. Owen WitteKeith Wolverton &

Carole LovingerJo Ann WoodsPatrick & Linda YatesJay & Carolyn YeskaJoyce Machaffie YoungMary Tonya YoungMervine YurekDan Zou

Kristel & Tim BorsosDr. Gerald & Sharon Tuck

Amy BurdBrian Burd

Cancer Patients EverywhereBig Sky Oncology

Dr. Grant & Deanna Harrer

Nancy & Ian DavidsonDean & Donna Wester

Bob DemingRoy & Donna AafedtRich Deming & Julie BennettHelen CoryJack & Mary DykstraJames & Cheryl Hirose

Bob & Lorraine DemingRobert & Lois HolterDorothy Jean McKayChris & Cindy Reiquam

FrankBob Wagner

Richard & Elizabeth GoffJustin, Nickie, Jackson & GraceMailletLarry & Delta MailletMGM Property Management

Specialists, LLCLouie & Christine Maillet

Helen IvesBen & Pat Ives

Dale LetzDonald Letz

Joe MazurekAnonymousRichard & Rayleen BeatonJohn BohlingerRobert BowmanJeanette BridgefordBret & Susan BrunnerSean & Patricia BurkeRobert & Marlene DillardTom & Marcia EckersonDenise FellerJohn & Aggie FrankinoAngus FultonSidney GoodrichRandy & Nora Flaherty GrayShane HedgesMarty & Gretchen HellerR. & Karen HodgeBernie & Kathy HubleyLinda JacquezCharles Johnson & Pat HuntMarie KallRobert & Susan KellerBarbara KennyJohn & Marcia MaynardJoe & Patricia MazurekMike & Joy McGrathDuane & Mary Moe

Colleen NicholsAlan & Nancy NicholsonJim & Anita OppedahlCatherine OrtweinFrank & Erica RamosDave & Joyce RobertsEmile & Letitia RuffierSandra SmithJerry & Martha SwansonJuanita TaylorRon & Mignon WatermanJoyce Machaffie Young

Joe & Patty MazurekDennis & Joan Taylor

Patty MazurekBarb O’ConnellSheryl Pressler

Nan McMillenJames & Deborah Arndt Heim

Nancy O’BrienDonna & Jack Lewis

Kathy RicePatrick Rice

Molly & Bruce UptonDavid & Margie Upton

Irv Weissman, MDDr. & Mrs. Owen Witte

Joan Woodcock (Birthday)Barbara Urquhart

22

Cliff AmdahlRalph & Alta Mae CoxSheila Eastman

Lt. Ken Anderson, CascadeCounty Sheriff’s DepartmentKeith Wolverton &

Carole Lovinger

Mary Ann ArnotEvelyn Munson

Robert C. ArnotRaymond & Kathleen Wherley

Robert C. & Mary Ann ArnotDebra Thatcher &

Dr. Nicholas Bonfilio

Shirlie BainMarge & Doug Bensley

Richard A. BergSheila Eastman

Alexander Blewett, IIFern Blewett

Marvine BowersDan & Madeline CarsonMargaret HardenDouglas & Barbara HugginsJames & Sharon JacobsonNancy & Carl LesmeisterNazdarBill PearsonSpencer PearsonJohn & Beverly Williams

Marvine & Thomas BowersDouglas & Barbara Huggins

Wanda L. BreedingMark & Sue ElingsMary BuntoDr. F. John & Robin Allaire

Nancy BurkeJohn & Betty Borchers

Luanna Hopson CatrettTest Support Group (Medical)Neutral Buoyancy Lab, NASA

Roy ChristiansenHarold HinmanRouns Land & Livestock, Inc.

Joel & Audrey Rouns

Helen ConnollyBruce Connolly

Ronald CookJanice Blom

John M. CrawfordPeggy Louise Crawford

Hoddy CunninghamRoy & Marian MeyerRobert & Betty Nelson

Robin Elaine DanielsSheila Eastman

Robert DaveyRichard & Carolyn Stremcha

Marge DavidsonLarry & Rebecca Williams

Jere B. DavisMargaret Davis

Katie DavisVernon & JoAnne Waldenberg

C. J. DennisRichard & Carol Marberg

Richard P. DewittNoella Goodell

Rosamund DiggsMark & Sue Elings

Tom DimkeCurt & Betty AmmondsonAnonymousLucille BalfourBarrick Electric

Leo & Marilyn BarrickBob & Jane BeckerW. L. & Jackie BourretOliver & Betty BucklinClifford & Walline CampbellTamara DimkeShirley EdwinDonald & Michele FoxBetty Hotaling & FamilyBarbara HovenSean & Andrea HovenJudy KinonenConnie LaabsRosemary ListonRichard & Carol MarbergJerome & Cecilia McConnellRichard & Karen McLaughlinRobert & Sherry MeadorsMitchell Development

& Investments, LLCTed Mitchell Family

Jeanne MooreDaniel MorganRell & Diana PorterWilliam & Nancy RamseyRockwell CollinsRon Hall Sprinklers

Ron HallJeanne & Chuck SchabelAlfred & Sally ShawRobert & Pat SlettenGene & Jane ThayerBob Wight

Alex DrapesTillie Kilby

Elliott DybdalLee & Terry Mongeon

Lyle & Gary DyerAnnie Dyer

Sue DyerHarvey & Joan Weikum

Alice DykstraNick & Patricia CettoIke & Mary Ann Kaufman

George “Sam” EhlangJane EhlangDavid & Diane KathmanKevin & Lynda Willms

Ernst Eichwald, MDSissy EichwaldDr. David & Patti Steinmuller

Marge ElerdingLarry & Rebecca Williams

Florence EllweinJames & Mary Larson

Irving & Sylvia FinemanEllen Fineman & Patrick Ward

Jim FlahertyMike & Fran MastersHarry & Kay Mitchell

Helen FowlerJohn & Betty Borchers

Lynn FreemanRoy & Marian Meyer

George H. GallagherRoberta GallagherBonnie Patterson

Dorothy GallickMarge & Doug Bensley

Fernande GarberDr. Peter & Irene Burleigh

Dr. H. C. GoodellShirley Jacobsen

Joanne GrahamJames & Mary Larson

Marsha Cotton HallRoberta Gallagher

Robert HallaOpal Halla

David HamiltonRoberta Gallagher

Harper & Leppien FamiliesJann & Ed Leppien

Betty HarperRobbie Gammack

Clarence HatchCarl & Anna May Erickson

Evelyn HauerNicholas & Evette Hauer

Joanna Baxter HendersonDennis & Mary Mollander

Jerry Lee HiattRuth Williams

Leigh HillDr. John & Betty Pfaff

John HindeJames Hinde, MD &

Lynn O’Malley, PhD

Gary E. HotalingBetty Hotaling

Dorothy, Robert, Judi &Michael HugginsDouglas & Barbara Huggins

Sharon HughesGlen & Leanna Coulter

Tom HurtleSandra Smith

E. W. “Gene” IvesBen & Pat Ives

Elda JacobsonSuper Enterprises, Inc.

Mr. & Mrs. James SuperMarian May

Nancy Lee JohnsonPeggy Louise Crawford

Stanley P. JohnsonVelma Johnson

Bessie KaltenbachHarlan & Patricia OrhamBarbara Urquhart

Anne KeyDr. F. John & Robin Allaire

Elizabeth Koski (mother)The Albrecht Family Trust

Darriel LachemannSheila Eastman

Arleen LargentDouglas & Barbara Huggins

Isabel (Pat) LesofskiRoger & Lois Nelson

Judy LiebetrauSheila Eastman

Darrell LindkeLorraine Burris

Sally LinnellJerrold & Nadyne Weissman

Andre LippensHarvey & Joan Weikum

Paul & Gladys Loendorf(Mom & Dad)

Edward & Susan Standley

Mary E. LoneyMike & Diana Delesha

Memorials

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Dorothy MarbergRichard & Carol Marberg

Pat MattixJames & Mary Larson

Lorna MayoLorna Mayo Family Foundation

Helen Louise McBridePeter & Sylvia Reiss

Richard J. McGeeKatherine McGee Benzel

Dr. John F. McGregorMary K. McGregor & Eric Schultz

Harriet McMasterMarian Hotvedt

William “Bill” MedvecJohn & Rosemary HusbyDarrell & Sharon KorinBruce & Debbie MedvecMervine Yurek

Linda MeeksBetty Hotaling

Ella MehmkeDr. Stephen Roth

E. R. “Chub” Munger John & Patty Cohlhepp

Edward A. NelsonRoger & Lois Nelson

Charles Bruce NewmanMichael Croskrey &

Elaine Schoyen

Tyson NimmickRobert & LeeAnn Hawkins

Elsie Schutz Bickel PartlowDonald & Niki Bickel

Suzy PetersHelen ClaryJean ClaryRichard A. ClaryRobert F. ClaryAnn Clary Gordon

Rosie PetersonMary Casey & Mike DavisMr. & Mrs. Jim ChristensenWarren & Nancy DrazichDarrell & Sharon KorinDeLores MillsJohn & Loretta PejkoMr. & Mrs. Jim RaphaelRich SohaPennie Vihinen

June PollingtonBonnie Mosbrucker

Gerald PottratzCarole LaValley

Bobby PrestonRichard & Carol Whitmore

Scott S. PreuningerRichard Knapp

Lorraine PrevostRobert & Charlotte Rahn

Donald L. PriceCarl & Nancy LesmeisterFred & Marge PrebleBarbara Tucker

E. Bruce PrivettAnonymousStephen & Ann DayDonald & Evelyn HoltRay & Lynn LouthenJayne & Maxwell MooreH.Dean&NancyJeanNewhouse

Robert RahnRobert & Charlotte Rahn

Daniel Carl RediskeDuElla Olson

Betty L. ReedNoella GoodellShirley JacobsenDuane Talcott

R. L. “Bob” ReiquamChris & Cindy Reiquam

Norris ReissenerAlan & Joyce Schubert

Joyce RobsonJo Ann Woods

Reece RowlingDebra Thatcher & Dr. Nicholas Bonfilio

Eloise RustvoldWilliam & Carol CrewsDiane NelsonAlda Nurmi

Evelyn SandersCheryl & Doug Aklestad

Roberta SchlageckMr. & Mrs. Aldo BarbagliaThe DILSBetsy JacobThomas & Barbara McGrathLaurie Sizemore

Allen SchmidtzMarie Boyd

Leroy SchottLoretta & Robert Pearce

Dick SchulerJay & Sheila Conlon

Margaret ShaferRichard & Molly Munro

Dr. William G. ShullScott & Lisa Shull

Vivian SiewingCarolyn & Jay Yeska

Carol SimonichJames & Mary Larson

Christine SkovronNorma Habel

Billie Lou SmithDorothy Willits

Daryl A. SolteszBetty Soltesz

Dr. Jack StimpflingArlyne ReichertDiana Talcott

Duane TalcottNoella GoodellSally McGregorRandy & Connie Peck

Alice TatchellSteve & Linda FranklinFriends of Alice Tatchell

LSU HSC Dept. of Biochemistry

Karla Gray & Myron Currie, JrShirley HudsonPhilip & Sherry IsherwoodR. W. & Kathryn McDonaldCynthia OverturfIvy Robertson

Dorothy TorgersonHarry Torgerson

C. Jack TurnerPhyllis A. Turner

Barbara UrquhartSheila EastmanCharles & Diana SchedelDr. Catherine Steele

Giulio & Emilia VanniJohn & Sandi Vanni

Anna M. WalbyBonnie Mosbrucker

Robert & Nora WardPatrick Ward & Ellen Fineman

Perle WeissmanMichael L. Smith

Judith WilliamsNoella Goodell

Phyllis WilliamsonRobert & Deloris Mau

Della WittRoy & Marian Meyer

For more information, call the Development Office at 406.454.6009.McLaughlin Research Institute’s Tax ID number is 81-045925.

Please use the enclosed envelope to make a donation by mail,or visit our website at mclaughlinresearch.org and go to Support MRI.

24

Statement of Financial Position June 30, 2012

Reproduced from the June 30, 2012 Financial Statements, auditedby Douglas Wilson & Company, PC. The complete audit report andfinancial statements are available on MRI’s website.

mclaughlinresearch.org

ASSETS

Cash and cash equivalents $ 13,779Grants & accounts receivable 308,670Prepaid expenses and supply inventories 64,224Investments 3,540,267Contributions receivable 192,384Other assets 5,187Property and equipment, net 10,133,021

TOTAL ASSETS $ 14,257,532

LIABILITIES & NET ASSETS

LIABILITIESAccounts payable $ 49,386Accrued liabilities 61,197Line of credit payable 200,000Grants received in advance 887,102Notes payable 251,671Gift annuity obligations 179,849

Total Liabilities 1,629,205

NET ASSETSUnrestricted / undesignated 9,145,526Unrestricted / board designated 1,307,653Temporarily restricted 57,304Permanently restricted 2,117,844

Total Net Assets 12,628,327

TOTAL LIABILITIES & NET ASSETS $ 14,257,532

Senior Research Assistant Rose Pitstick is co-author with Dr. Carlson on many of the scientific publicationsthat advance knowledge on Alzheimer’s and prion diseases.

Non-Profit Organization

U.S. POSTAGE

PAID

Great Falls, Montana

Permit No. 207McLaughlin Research Institute

for Biomedical Sciences

1520 23rd Street SouthGreat Falls, MT 59405

Phone 406.452.6208 / Fax 406.454.6019

mclaughlinresearch.org

“Our goal is to improve patient care … the reason we do research is to improve

the health of people across the world and in this community.”

–Dr. George Carlson