A

B

C

Control DFPM DFPM-2 DFPM-3 DFPM-5 DFPM-7DFPM-1Cl

Cl

O

S

N

Cl

Cl

O

S

N

O

Cl

O

S

N

Cl

Cl

O

S

N

O

N

O

S

N

Cl

O

S

N

Cl

Control DFPM-2 DFPM-3 DFPM-5DFPM DFPM-7DFPM-1

**

*

35S::VICTRCol-0 vector controlD

Supplemental Figure 1. Functional characterization of modified DFPM structures and 35S::VICTRCol ectopic expression caused stunted growth (A) Chemical structures of DFPM derivatives. (B) DFPM-induced root growth arrest phenotype was compared to roots treated with DFPM derivatives. Col-0 seedlings in each panel were treated with 10 µM of the chemical presented above. (C) Quantitative analyses of root growth arrest induced by DFPM derivatives as shown in B.Error bars mean ± s.d.. * denotes P<0.02 (D) 35S::VICTRCol ectopic expression in Col-0 caused

Supplemental Data. Kim et al. Plant Cell. (2012). 10.1105/tpc.112.107235

1

0.5 h 1 h 2 h 4 h 8 h0 h 24 h

PIN

2:G

FPPI

N1:

GFP

10 µM DFPMControl 8 h 18 h 24 h

A B

DR5 SCR CyclinB1

C

Supplemental Figure 2. Gene expression and morphological changes of DFPM-treated seed-lings. (A) DR5, SCR and CyclinB1 gene expression patterns were not affected 1 day after DFPM treatment. (B) Effect of DFPM in PIN1:GFP and PIN2:GFP fusion protein expression in the primary root. Fluorescence intensities of PIN1:GFP and PIN2:GFP become weaker after 18 hours of DFPM treatment and negligible at 24 hours of DFPM treatment. Images are representatives from at least 10 plants per condition per strain with similar results. Red fluorescence is propidium iodide staining. (C) The VICTR phenotype is irreversible after 8 hours of DFPM treatment. After treating 10 day-old Col-0 seedlings with 10 µM DFPM for the indicated times, seedlings were transferred to normal medium to analyze recovery of primary root growth.

Supplemental Data. Kim et al. Plant Cell. (2012). 10.1105/tpc.112.107235

2

victr-2victr-1Col-0

0

5

10

15

20

25

30

victr-2

Mer

iste

mat

ic c

orte

x ce

lls

victr-1Col-0

0 µM DFPM10 µM DFPM

*

B

A

Supplemental Figure 3. DFPM reduces the number of meristem cells in Col-0, but not in victrmutant alleles. (A) Confocal images of 6-day-old roots stained with propidium iodide. Seedlings were grown for 5 days on 0.5 X MS media and transferred to 10 µM DFPM for 24 hours before primary roots were imaged. Arrows represent the boundary between the elongation zone and the proximal meristem of the primary root. (B) Quantification of cortex meristem cell numbers in the division zone. Asterisk indi-cates statistically significant differences (P<0.05; ANOVA, Tukey test; n=10).

Supplemental Data. Kim et al. Plant Cell. (2012). 10.1105/tpc.112.107235

3

A B

VICTR-YFP YFP-VICTRYFP

CLer Colvictl1-1

D

Ler eds5-1 eds1-230

10

20

30

400 µM DFPM

10 µM DFPM

Prim

ary

root

gro

wth

[mm

]Ler eds5-1 eds1-23

E

Supplemental Figure 4. Characterization of eds1-23 T-DNA insertion line and victl1-1. Subcellu-lar localization of VICTR, and VICTR deletion in other accessions. (A) eds1-23 T-DNA insertion line is DFPM resistant Error bars mean ± s.d.. (n=3 with 10 plants each) (B) Phylogenetic tree of VICTR subfamily. Numbers in red present branchsupport values. (C) Full sensitivity of victl1-1(Salk_097845) to DFPM treatment confirms that VICTL1, the most similar gene to VICTR is not required for the DFPM-induced root response. (D) Subcellular localization of VICTR-YFP and YFP-VICTR in onion epidermal cells. YFP alone was used as a control. (E) PCR analysis suggests that VICTR is deleted in the WS accession like in Ler. Internal primers matching to sequences of the VICTRgene are 1.5 kb apart and external primers each annealing to upstream and downstream sequences of the VICTR gene, respectively are 24 kb apart in Col.

0.2

AT5G46260.1

AT5G46450.1

AT5G46270.1

AT1G31540.2

AT5G22690.1

AT5G46510.1

AT5G46490.2

AT5G46520.1

AT5G46470.1

848

946

1000

980

1000

1000

919

1000

VICTR

VICTR1

AT4G08450.1

Supplemental Data. Kim et al. Plant Cell. (2012). 10.1105/tpc.112.107235

4

Kin-0 1 M A S S S S S R NWV Y D V F L S F S G K D V R V T F R S H F L K E L D R K L I S A F R D N E I E RBay-0 1 M A S S S S S R NWV Y D V F L S F S G K D V R V T F R S H F L K E L D R K L I S A F R D N E I E RCol-0 1 M A S S S S S R NWV Y D V F L S F S G K D V R V T F R S H F L K E L D R K L I S A F R D N E I E R

Kin-0 51 S H S L WP D L E Q A I K D S R I A V V V F S K N Y A S S SWC L N E L L E I V N C N D K I I I P VBay-0 51 S H S L WP D L E Q A I K D S R I A V V V F S K N Y A S S SWC L N E L L E I V N C N D K I I I P VCol-0 51 S H S L WP D L E Q A I K D S R I A V V V F S K N Y A S S SWC L N E L L E I V N C N D K I I I P V

Kin-0 101 F Y G V D P S Q V R Y Q I G E F G S I F E K T C K R Q T E E V K N QWK K A L T D V A NM L G F D SBay-0 101 F Y G V D P S Q V R Y Q I G E F G S I F E K T C K R Q T E E V K N QWK K A L T D V A NM L G F D SCol-0 101 F Y G V D P S Q V R Y Q I G E F G S I F E K T C K R Q T E E V K N QWK K A L T D V A NM L G F D S

Kin-0 151 A KWD D E A K M I E E I A N D V L G K L L L T S S T D S A E N S I G I E D H I A NM S V L L Q L EBay-0 151 A KWD D E A K M I E E I A N D V L G K L L L T S S T D S A E N S I G I E D H I A NM S V L L Q L ECol-0 151 A KWD D E A K M I E E I A N D V L A K L L L T S S T D S A E N S I G I E D H I A NM S V L L K L E

* *Kin-0 201 A E E V RM V G I WG S S G I G K T T I A R A L F N Q L S R H F P V S K F I D R A F V S N C R E T YBay-0 201 A E E V RM V G I WG S S G I G K T T I A R A L F N Q L S R H F P V S K F I D R A F V S N C R E T YCol-0 201 A E E V RM V G I WG S S G I G K T T I A R A L F N Q L S R H F P V S K F I D R A F V Y K S R E T Y

* * *Kin-0 251 K G A N P D D P NM K L H L Q G C F L S E I L G K K D I K I G H L G A L R E R L K H H K T L I I I DBay-0 251 K G A N P D D P NM K L H L Q G C F L S E I L G K K D I K I G H L G A L R E R L K H H K T L I I I DCol-0 251 K G A N P D D P NM K L H L Q G C F L S E I L G K K D I K I D H L G A L G E R L K H Q K T L I I I D

* * *Kin-0 301 D L D D L V V L D T L V G K T NW F G C G S R I I V I T N N K Q F L R A H G I D H I Y E V S L P S KBay-0 301 D L D D L V V L D T L V G K T NW F G C G S R I I V I T N N K Q F L R A H G I D H I Y E V S L P S KCol-0 301 D L D D L V V L D S L V G K T NW F G C G S R I I V I T N N K Q F L R A H G I D H I Y E V S L P S K

*Kin-0 351 E R A Q E M F C Q S A F G E N S P P E G F E E L V V E I AW L A G S L P L G L T V F G S A L R G R KBay-0 351 E R A Q E M F C Q S A F G E N S P P E G F E E L V V E I AW L A G S L P L G L T V F G S A L R G R KCol-0 351 E R A Q E M F C Q S A F G E N S P P E G F E E L V V E I AW L A G S L P L G L T V F G S A L R G R K

Kin-0 401 K E YWV K M L P R L Q N D L D G N I E T T L K V S Y D A I G N V K D Q A L F R H I A C L F N H V KBay-0 401 K E YWV K M L P R L Q N D L D G N I E T T L K V S Y D A I G N V K D Q A L F R H I A C L F N H V KCol-0 401 K E YWV K M L P R L Q N D L D G N I E E T L K V S Y D A I G N V K D Q A L F R L I A C L F N H V K

* *Kin-0 451 V R D I E L L L A D S G L D V N I A R E N L V D K S L I H V R N E H V E M H R L L Q E T G R N I V RBay-0 451 V R D I E L L L A D S G L D V N I A R E N L V D K S L I H V R N E H V E M H R L L Q E T G R N I V RCol-0 451 V R D I E L L L A D S G L D V N I A L E N L V D K S L I H V R N D H V E M H R L L Q E T G R N I V R

* *Kin-0 501 L Q S I D N P G E R E F L V D - Y D S R T V L S E G I G T R K V L G I S L D T S K V S E F C V H E NBay-0 501 L Q S I D N P G E R E F L V D - Y D S R T V L S E G I G T R K V L G I S L D T S K V S E F C V H E NCol-0 501 S Q S T D N P G E R E F L V D S N D S R T V L S E G I G T R K V L G I S L D T S K V S E F C V H E N

* * * *Kin-0 550 A F K GM C N L L F L D I F S S T S Y T L E E D E E E K V R V Q L P E K L N Y Y S V Q L K L L I WEBay-0 550 A F K GM C N L L F L D I F S S T S Y T L E E D E E E K V R V Q L P E K L N Y Y S V Q L K L L I WECol-0 551 A F K GM G N L L F L D I S S - - - - - - K T F I E E E V K V H L P E K I N Y Y S V Q P K Q L I WD

* * * * * * * * * * * * * * * * * * *Kin-0 600 R F P L K CM P S T Y L R N L V K L K M H E S K L E K L WE G A M S F T C L K E L DMWA S K Y L KBay-0 600 R F P L K CM P S T Y L R N L V K L K M H E S K L E K L WE G A M S F T C L K E L DMWA S K Y L KCol-0 595 R F P L K CM P Y T F L R N L V K L E M H D S K L E K L WE G A M S F T C L K E L DMWA S K Y L K

* * * *Kin-0 650 E I P D L S K A T N I E K L D F G H CWS L V E L P S S I R N L N K L L E L NM E Y C G E L E T L PBay-0 650 E I P D L S K A T N I E K L D F G H CWS L V E L P S S I R N L N K L L E L NM E Y C G E L E T L PCol-0 645 E I P D L S K A T N I E K L D F G H CWS L V E L P S S I R N L N K L L E L NM E Y C G E L E T L P

Kin-0 700 T G F N L K S L D Y L N F N E CWK L R T F P E F A T N I S N L I L A E T S I E E Y P S N L Y F K NBay-0 700 T G F N L K S L D Y L N F N E CWK L R T F P E F A T N I S N L I L A E T S I E E Y P S N L Y F K NCol-0 695 T G F N L K S L D Y L N F N E CWK L R T F P E F A T N I S N L I L A E T S I E E Y P S N L Y F K N

Kin-0 750 V R E L S MGK A DS D E N K C QGV K P F M P M L S P T L T L L E L WN I P N L V E L S S S F QNBay-0 750 V R E L S MGK A DS D E N K C QGV K P F M P M L S P T L T L L E L WN I P N L V E L S S S F QNCol-0 745 V R E L S MGK A DS D E N K C QGV K P F M P M L S P T L T L L E L WN I P N L V E L S S S F QN

Kin-0 800 L N N L E R L D I C Y C R N L E S L P T G I N L E S L V S L N L F G C S R L K R F P D I S T N I K YBay-0 800 L N N L E R L D I C Y C R N L E S L P T G I N L E S L V S L N L F G C S R L K R F P D I S T N I K YCol-0 795 L N N L E R L D I C Y C R N L E S L P T G I N L E S L V S L N L F G C S R L K R F P D I S T N I K Y

Kin-0 850 L D L DQ T G I E E V PWQ I E N F F N L T K L T MK GC R E L K C V S L N I F K L K H L G E V S FBay-0 850 L D L DQ T G I E E V PWQ I E N F F N L T K L T MK GC R E L K C V S L N I F K L K H L G E V S FCol-0 845 L D L DQ T G I E E V PWQ I E N F F N L T K L T MK GC R E L K C V S L N I F K L K H L G E V S F

Kin-0 900 S N C GA L T R V D L S C Y P S GV EMMK A DN A D I V S E E T T S S L P DS C V L N V N F MDCBay-0 900 S N C GA L T R V D L S C Y P S GV EMMK A DN A D I V S E E T T S S L P DS C V L N V N F MDCCol-0 895 S N C GA L T R V D L S C Y P S GV EMMK A DN A D I V S E E T T S S L P DS C V L N V N F MDC

Kin-0 950 V N L DR E P V L H QQS I I F N S M I L P G E E V P S Y F T Y R T S DS Q P F G T S S S L P I P LBay-0 950 V N L DR E P V L H QQS I I F N S M I L P G E E V P S Y F T Y R T S DS Q P F G T S S S L P I P LCol-0 945 V N L DR E P V L H QQS I I F N S M I L P G E E V P S Y F T Y R T S DS Q P F G T S S S L P I P L

Kin-0 1000 L P T Q L S Q P F F R F R V C A V V S A S N GV Y I GV Y S R F K GR I GN K F DS F G E L H K F MBay-0 1000 L P T Q L S Q P F F R F R V C A V V S A S N GV Y I GV Y S R F K GR I GN K F DS F G E L H K F MCol-0 995 L P T Q L S Q P F F R F R V C A V V S A S N GV Y I GV Y S R F K GR I GN K F DS F G E V H N F M

* *Kin-0 1050 E I E K G I H L C I F D C R I R L Y K D N V P L S Q L N Y D H V D I N I H I T S G DWR S T V V L KBay-0 1050 E I E K G I H L C I F D C R I R L Y K D N V P L S Q L N Y D H V D I N I H I T S G DWR S T V V L KCol-0 1045 E I E K G I H L C I F D C R I R L Y K D N V P L S Q L N Y D H V D I N I H I T S G DWR S T V V L K

Kin-0 1100 EWG I R L L E D C S S A E N R L G N P N S T L P H V S Q A E E G NM G Y Y T H V Q G L V N E I E NBay-0 1100 EWG I R L L E D C S S A E N R L G N P N S T L P H V S Q A E E G NM G Y Y T H V Q G L V N E I E NCol-0 1095 EWG I R L L E T G S S A E N R L G N P N S T L P H V S Q A E E G NM G Y Y T H V Q G L V N E I E N

* *Kin-0 1150 S E D S G D N N V E T E R S K K RM R L H H F IBay-0 1150 S E D S G D N N V E T E R S K K RM R L H H F ICol-0 1145 S E D S G D N N V E T E R S K K RM R L H H F I

Bay-promoter -2495 GAGAACCAGTACACAATGGCAGGTAAGTATTAATCAAGAACTGAACAGAATAACAGATATCTCGCCGTCAGGATCTTTAAKin-promoter -2495 GAGAACCAGTACACAATGGCAGGTAAGTATTAATCAAGAACTGAACAGAATAACAGATATCTCGCCGTCAGGATCTTTAACol-promoter -2496 GAGAACCAGTACACAATGGCAGGTAAGTATTAATCAAGAACTGAACAGAATAACAGATATCTCGCCGTCAGGATCTTTAA

Bay-promoter -2415 TATTATAAATCACTGAGTTGTTTTCTTTTGCTTGCAGATTATGTGAAAAAGAAATGAATCTTTGAGATACTTTTGTGGTGKin-promoter -2415 TATTATAAATCACTGAGTTGTTTTCTTTTGCTTGCAGATTATGTGAAAAAGAAATGAATCTTTGAGATACTTTTGTGGTGCol-promoter -2416 TATTATAAATCACTGAGTTGTTTTCTTTTGCTTGCAGATTATGTGAAAAAGAAATGAATCTTTGAGATACTTTTGTGGTG

Bay-promoter -2355 ATGGAATGAGTAGTTTTGCTGCCGGTTCTATGGAACAACAAGAAGAGCCCAAGATTCCTCATTGTCTCCCTAGCTAGACAKin-promoter -2355 ATGGAATGAGTAGTTTTGCTGCCGGTTCTATGGAACAACAAGAAGAGCCCAAGATTCCTCATTGTCTCCCTAGCTAGACACol-promoter -2356 ATGGAATGAGTAGTTTTGCTGCCGGTTCTATGGAACAACAAGAAGAGCCCAAGATTCCTCATTGTCTCCCTAGCTAGACA

Bay-promoter -2275 GACATTTTCAGGTGACCCAAAAAAACTCATAAACCATTTCATTTTAGTTCCACATCAATTGTAAATACAGAGCATATATAKin-promoter -2275 GACATTTTCAGGTGACCCAAAAAAACTCATAAACCATTTCATTTTAGTTCCACATCAATTGTAAATACAGAGCATATATACol-promoter -2276 GACATTTTCAGGTGACCCAAAAAAACTCATAAACCATTTCATTTTAGTTCCACATCAATTGTAAATACAGAGCATATATA

Bay-promoter -2195 TAGTAGCATGCTTTTGAATTTCTGATTGTCTGGTTATAGACTTTTTATTATATAGATATTTGGCTGTATTCAATTGATAAKin-promoter -2195 TAGTAGCATGCTTTTGAATTTCTGATTGTCTGGTTATAGACTTTTTATTATATAGATATTTGGCTGTATTCAATTGATAACol-promoter -2196 TAGTAGCATGCTTTTGAATTTCTGATTGTCTGGTTATAGACTTTTTATTATATAGATATTTGGCTGTATTCAATTGATAA

Bay-promoter -2115 ATTTCACAGCCATCAATGTTGTTCACTGCTTGGTTAAAGACTTTGATATTATCTATTATGGGATAATTAGATACATGTGTKin-promoter -2115 ATTTCACAGCCATCAATGTTGTTCACTGCTTGGTTAAAGACTTTGATATTATCTATTATGGGATAATTAGATACATGTGTCol-promoter -2116 ATTTCACAGCCATCAATGTTGTTCACTGCTTGGTTAAAGACTTTGATATTATCTATTATGGGATAATTAGATACATGTGT

Bay-promoter -2035 GCTAGTATTATGAGGCATATATTTCAAAAAAAAGTCGTGATGTAGTTACAAAAATTGATGGAATTCAAGCCACTGGTTTTKin-promoter -2035 GCTAGTATTATGAGGCATATATTTCAAAAAAAAGTCGTGATGTAGTTACAAAAATTGATGGAATTCAAGCCACTGGTTTTCol-promoter -2036 GCTAGTATTATGAGGCATATATTTCAAAAAAAAGTCGTGATGTAGTTACAAAAATTGATGGAATTCAAGCCACTGGTTTT

Bay-promoter -1955 TAATTAGATTATAAAGCTGAAGGAATGGAATTGTCTCAAATGGCAGATGAAGATGGTACTGTTCATGTCAATGTTTTATAKin-promoter -1955 TAATTAGATTATAAAGCTGAAGGAATGGAATTGTCTCAAATGGCAGATGAAGATGGTACTGTTCATGTCAATGTTTTATACol-promoter -1956 TAATTAGATTATAAAGCTGAAGGAATGGAATTGTCTCAAATGGCAGATGAAGATGGTACTGTTCATGTCAATGTTTTATA

Bay-promoter -1875 ATTTTTTGTTTTTTTTCTTAGGTTGCACGTTTGTGATAAGCTTATTTCATTACTATAACGATTTAATTTCTGAAGAACTAKin-promoter -1875 ATTTTTTGTTTTTTTTCTTAGGTTGCACGTTTGTGATAAGCTTATTTCATTACTATAACGATTTAATTTCTGAAGAACTACol-promoter -1876 ATTTTTTGTTTTTTTTCTTAGGTTGCACGTTTGTGATAAGCTTATTTCATTACTATAACGATTTAATTTCTGAAGAACTA

Bay-promoter -1795 TTTGTGTTATTTGTGTTCACAAGAATTTGCTGTCTGGAACAAAGTTTTACAGTGTACCTGAATTGAGTTTTCTTTTATTCKin-promoter -1795 TTTGTGTTATTTGTGTTCACAAGAATTTGCTGTCTGGAACAAAGTTTTACAGTGTACCTGAATTGAGTTTTCTTTTATTCCol-promoter -1796 TTTGTGTTATTTGTGTTCACAAGAATTTGCTGTCTGGAACAAAGTTTTACAGTGTACCTGAATTGAGTTTTCTTTTATTC

Bay-promoter -1715 GTTTTCGGATTTCACTCTCCACTAGTTGAAAAAGGTCTGAGGCTTTCTAGATTGTTTCAAACTTCGTTAGGAACATGAAAKin-promoter -1715 GTTTTCGGATTTCACTCTCCACTAGTTGAAAAAGGTCTGAGGCTTTCTAGATTGTTTCAAACTTCGTTAGGAACATGAAACol-promoter -1716 GTTTTCGGATTTCACTCTCCACTAGTTGAAAAAGGTCTGAGGCTTTCTAGATTGTTTCAAACTTCGTTAGGAACATGAAA

Bay-promoter -1635 ATTTGCATCTAGACAATGCCCTGTGGTATCTGGTAATCAAAATTCTGTTTCTGGTTACTTCGTTTTTACATGGGCAGAAAKin-promoter -1635 ATTTGCATCTAGACAATGCCCTGTGGTATCTGGTAATCAAAATTCTGTTTCTGGTTACTTCGTTTTTACATGGGCAGAAACol-promoter -1636 ATTTGCATCTAGACAATGCCCTGTGGTATCTGGTAATCAAAATTCTGTTTCTGGTTACTTCGTTTTTACATGGGCAGAAA

Bay-promoter -1555 GTGAATCTCCTACAGGTCTTGCGACACAGTTAGCTTTTGATTTGATAAGCTTACTGTTACTTTAACCCTGTTGACATTTCKin-promoter -1555 GTGAATCTCCTACAGGTCTTGCGACACAGTTAGCTTTTGATTTGATAAGCTTACTGTTACTTTAACCCTGTTGACATTTCCol-promoter -1556 GTGAATCTCCTACAGGTCTTGCGACACAGTTAGCTTTTGATTTGATAAGCTTACTGTTACTTTAACCCTGTTGACATTTC

Bay-promoter -1475 TCACCTATGTCATTCGTTTGAAAGCTTGTTTTGATTCTCTAATGATATGCTTAACTTTCATTCTCTGATGATTCTTTTGTKin-promoter -1475 TCACCTATGTCATTCGTTTGAAAGCTTGTTTTGATTCTCTAATGATATGCTTAACTTTCATTCTCTGATGATTCTTTTGTCol-promoter -1476 TCACCTATGTCATTCGTTTGAAAGCTTGTTTTGATTCTCTAATGATATGCTTAACTTTCATTCTCTGATGATTCTTTTGT

Bay-promoter -1395 CTTGCAATTGTTATTGTTCAGGAAAGCTGGAGGAAGTCACTTTGATGACCAATGTTGGTTACGGATCTGACAAGATATTAKin-promoter -1395 CTTGCAATTGTTATTGTTCAGGAAAGCTGGAGGAAGTCACTTTGATGACCAATGTTGGTTACGGATCTGACAAGATATTACol-promoter -1396 CTTGCAATTGTTATTGTTCAGGAAAGCTGGAGGAAGTCACTTTGATGACCAATGTTGGTTACGGATCTGACAAGATATTA

Bay-promoter -1315 ATGTAAGAATAAGAGGCTATGGACATGGTTTTTACTTGAGTGGTGGCGATTAAGTCCAAATCTAAAAGAGATGATTAAGTKin-promoter -1315 ATGTAAGAATAAGAGGCTATGGACATGGTTTTTACTTGAGTGGTGGCGATTAAGTCCAAATCTAAAAGAGATGATTAAGTCol-promoter -1316 ATGTAAGAATAAGAGGCTATGGACATGGTTTTTACTTGAGTGGTGGCGATTAAGTCCAAATCTAAAAGAGATGATTAAGT

Bay-promoter -1235 CAAGATCCAGCAGAAAAGAGGAAACTTTCGTTAAGGTGTATGACAACAGTGGTGCAATTGCTAATGTTTGTGAATTGGTCKin-promoter -1235 CAAGATCCAGCAGAAAAGAGGAAACTTTCGTTAAGGTGTATGACAACAGTGGTGCAATTGCTAATGTTTGTGAATTGGTCCol-promoter -1236 CAAGATCCAGCAGAAAAGAGGAAACTTTCGTTAAGGTGTATGACAACAGTGGTGCAATTGCTAATGTTTGTGAATTGGTC

Bay-promoter -1155 TTTTTTCTTATTAGGGATATATAATGAAGCAAGCATGATTGAAGAAATCGCCAAGTCTTAATCGGCTCACGTTAGGTGGTKin-promoter -1155 TTTTTTCTTATTAGGGATATATAATGAAGCAAGCATGATTGAAGAAATCGCCAAGTCTTAATCGGCTCACGTTAGGTGGTCol-promoter -1156 TTTTTTCTTATTAGGGATATATAATGAAGCAAGCATGATTGAAGAAATCGCCAAGTCTTAATCGGCTCACGTTAGGTGGT

Bay-promoter -1075 GGAAAGACGACGCATTGCAAAAGTTTTGCAGGCTGACGAAAAAAGAGGAGAATGGAGATCAATTGGAAGAGGTTCTTCTTKin-promoter -1075 GGAAAGACGACGCATTGCAAAAGTTTTGCAGGCTGACGAAAAAAGAGGAGAATGGAGATCAATTGGAAGAGGTTCTTCTTCol-promoter -1076 GGAAAGACGACGCATTGCAAAAGTTTTGCAGGCTGACGAAAAAAGAGGAGAATGGAGATCAATTGGAAGAGGTTCTTCTT

Bay-promoter -995 ATTTGATTGATTAATTTTTCTTTTCTTTCTATATTTAGATATACATGGTGACACCCATTATTGTCCAATTTGTCCCTTTTKin-promoter -995 ATTTGATTGATTAATTTTTCTTTTCTTTCTATATTTAGATATACATGGTGACACCCATTATTGTCCAATTTGTCCCTTTTCol-promoter -996 ATTTGATTGGTTAATTTTTCTTTTCTTTCTATATTTAGATATACATGGTGACACCCATTATTGTCCAATTTGTCCCTTTT

*Bay-promoter -915 TTCTGTTTCTTTTGAGACCATGTCTATATATGAGAATTGAGAATCTTACAATTAAATATTTTGAACTAATAAGTCTTTTAKin-promoter -915 TTCTGTTTCTTTTGAGACCATGTCTATATATGAGAATTGAGAATCTTACAATTAAATATTTTGAACTAATAAGTCTTTTACol-promoter -916 TTCTGTTTCTTTTGAGACCATGTCTATATATGAGAATTGAGAATCTTACAATTAAATATTTTGAACTAATAAGTCTTTTA

Bay-promoter -835 TTTTGTTTCCAACTCATGTGTTTAACTAAGTCCTAAGCTAACACTAGATTTTTTTTAAAAGAAATTTGGTTTTCCTCCACKin-promoter -835 TTTTGTTTCCAACTCATGTGTTTAACTAAGTCCTAAGCTAACACTAGATTTTTTTTAAAAGAAATTTGGTTTTCCTCCACCol-promoter -836 TTTTGTTTCCAACTCATGTGTTTAACTAAGTCCTAAGCTAACACTAGATTTTTTTTAAAAGAAATTTGGTTTTCCTCCAC

Bay-promoter -755 AGTATTTTTATTGTTTTTTGTGGACTTGACCACAAAACTTCAAAAGACTCGGAAGAAAATTAAACAAATATTATGCACAAKin-promoter -755 AGTATTTTTATTGTTTTTTGTGGACTTGACCACAAAACTTCAAAAGACTCGGAAGAAAATTAAACAAATATTATGCACAACol-promoter -756 AGTATTTTTATTGTTTTTTGTGGACTTGACCACAAAACTTCAAAAGACTCGGAAGAAAATTAAACAAATATTATGCACAA

Bay-promoter -675 TTTCGCACCACTCAATGCCTTGCCTCCCCTACCTAAAATGTTGACAATGTCGTCTTATTCTTTATGTCAAGCACGATGATKin-promoter -675 TTTCGCACCACTCAATGCCTTGCCTCCCCTACCTAAAATGTTGACAATGTCGTCTTATTCTTTATGTCAAGCACGATGATCol-promoter -676 TTTCGCACCACTCAATGCCTTGCCTCCCCTACCTAAAATGTTGACAATGTCGTCTTATTCTTTATGTCAAGCACGATGAT

Bay-promoter -595 GTGTCTGACTTTAAGTAATTTACATTTTTTTAAAATTCGTGATAGATATTCTCCCAAATTCTTTCAAATATCATAGGCTTKin-promoter -595 GTGTCTGACTTTAAGTAATTTACATTTTTTTAAAATTCGTGATAGATATTCTCCCAAATTCTTTCAAATATCATAGGCTTCol-promoter -596 GTGTCTGACTTTAAGTAATTTACATTTTTTAAAAATTCGTGATAGATATTCTCCCAAATTCTTTCAAATATCATAGGCTT

*Bay-promoter -515 TAAGATGTTACCAACTACCAAGTCTCCTAAAAAGACCAAAAAGTCTCCTTAGATATTTATCAAACTCTTTGATGTTTTGTKin-promoter -515 TAAGATGTTACCAACTACCAAGTCTCCTAAAAAGACCAAAAAGTCTCCTTAGATATTTATCAAACTCTTTGATGTTTTGTCol-promoter -516 TAAGATGTTACCAACTACCAAGTCTCCTAAAAAGACCAAAAAGTCTCCTTAGATATTTATCAAACTCTTTGATGTTTTGT

Bay-promoter -435 TGCTCGTTCCTTCGTTCACAGTTTCCTGATTTAACAGTG - CCAGTAGGATAATGATTACGTTGCTTCACTTTTCACTGTTKin-promoter -435 TGCTCGTTCCTTCGTTCACAGTTTCCTGATTTAACAGTG - CCAGTAGGATAATGATTACGTTGCTTCACTTTTCACTGTTCol-promoter -436 TGCTCGTTCCTTCGTTCACAGTTTCCTGATTTAACAGTGGCCAGTAGGATAATGATTACGTTGCTTCACTTTTCACTGTT

*Bay-promoter -356 ACTGTGGAATTGCTTGGTAATTTCGAAAACTTGTATCCTTATTAAGAATTTGTAAAAAGTTTTGCAACTTCGTATTGTACKin-promoter -356 ACTGTGGAATTGCTTGGTAATTTCGAAAACTTGTATCCTTATTAAGAATTTGTAAAAAGTTTTGCAACTTCGTATTGTACCol-promoter -356 ACTGTGGAATTGCTTGGTAATTTCGAAAACTTGTATCCTTATTAAGAATTTGTAAAAAGTTTTGCAACTTCGTATTGTAC

Bay-promoter -276 ATCTTTATTAAGAATTTGTAAATGAATGATATACAGTAGTAAAGACAAGAGGCTATGAAATATGGTTATGACGATTGAGTKin-promoter -276 ATCTTTATTAAGAATTTGTAAATGAATGATATACAGTAGTAAAGACAAGAGGCTATGAAATATGGTTATGACGATTGAGTCol-promoter -276 ATCTTTATTAAGAATTTGTAAATGAATGATATACAGTAGTAAAGACAAGAGGCTATGAAATATGGTTATGACGATTGAGT

Bay-promoter -196 CAACAAAGAGGAAACTTCCGCCAAGTAAAAGTCTAAGAAATAATTGTGAAAACTTGTATGGCCAAGTTACCTCTCAGCTGKin-promoter -196 CAACAAAGAGGAAACTTCCGCCAAGTAAAAGTCTAAGAAATAATTGTGAAAACTTGTATGGCCAAGTTACCTCTCAGCTGCol-promoter -196 CAACAAAGAGGAAACTTCCGCCAAGTAAAAGTCTAAGAAATAATTGTGAAAACTTGTATGGCCAAGTTACCTCTCAGCTG

Bay-promoter -116 TTATGTTAAATGTTTTATATCCACCAAGACAGACTCACTACTTGTCCTCTATTTCTCTTCTTAAAATCTCAATCTCCAAAKin-promoter -116 TTATGTTAAATGTTTTATATCCACCAAGACAGACTCACTACTTGTCCTCTATTTCTCTTCTTAAAATCTCAATCTCCAAACol-promoter -116 TTATGTTAAATGTTTTATATCCACCAAGACAGACTCACTACTTGTCCTCTATTTCTCTTCTTAAAATCTCAATCTCCAAA

Bay-promoter -36 GTCTTCGTTTCTAGTTTCCTCCTTCTAACTCTCTCTATGKin-promoter -36 GTCTTCGTTTCTAGTTTCCTCCTTCTAACTCTCTCTATGCol-promoter -36 GTCTTCGTTTCTAGTTTCCTCCTTCTAACTCTCTCTATG

BA

Supplemental Figure 5. VICTR gene sequence comparison of Col-0 with Bay-0 and Kin-0. (A) Amino acid sequences were deduced by sequencing of full-length VICTR cDNAs from Col-0, Bay-0, and Kin-0. Amino acid polymorphisms specifically found in VICTRCol are marked by asterisks. (B)Sequence comparison of VICTRCol promoter with VICTRBay and VICTRKin promoters. Asterisks denote variations among all three sequences.

Supplemental Data. Kim et al. Plant Cell. (2012). 10.1105/tpc.112.107235

5

nVen

us-G

US

+

cCFP

-VIC

TR

nVen

us-V

ICTR

+

cCFP

-GU

S

nVen

us-E

DS

1 +

cCFP

-PA

D4

nV

enus

-PA

D4

+ cC

FP-E

DS

1

* *

* * *

*

170

130

100

70

cCFP-VICTR + nVenus-VICTR +

A B YFP-filter RFP-filter Overlay

nVen

us-E

DS1

nVen

us-P

AD4

nVen

us-G

US

cCFP-E

DS1

cCFP-P

AD4

cCFP-G

US

IB: αGFP

Supplemental Figure 6. VICTR does not associate with GUS. (A) No association of VICTR protein was found in BiFC analyses using GUS as a control. Experiments were performed as decribed in Figure 6. (B) Expression of each protein was confirmed by immunodetection in Western Blots.

Supplemental Data. Kim et al. Plant Cell. (2012). 10.1105/tpc.112.107235

6

DFPM

ControlLer

A

B

Col-0 LerSA 30 µM

Col-0 Ler Col-0SA 300 µM

SA

Supplemental Figure 7. Root growth inhibition by salicylic acid (SA) differs from the DFPM-induced primary root growth arrest response. (A) At 300 μM, SA inhibits general growth regard-less of Arabidopsis strains. (B) The primary root specific growth arrest by DFPM treatment observed in Col-0 is distinct from SA treated roots.

Supplemental Data. Kim et al. Plant Cell. (2012). 10.1105/tpc.112.107235

7

Strain ABRC stock # Strain ABRC stock #An-1 CS22626 LL-0 CS22650Bay-0 CS22633 Lm-2 CS1344Be-0 CS6613 Mr-0 CS22640Bil-5 CS22578 Mt-0 CS22642Bor-1 CS22590 Mz-0 CS22636Br-0 CS22628 Nd-0 CS6803Bur-0 CS22656 No-0 CS6805C24 CS22620 Nok-3 CS22643CSHL-1 CS22419 Oy-0 CS22658Cvi-0 CS22614 Pro-0 CS22649Di-0 CS6680 Ra-0 CS22632Eden-1 CS22572 Ren-1 CS22610Edi-0 CS22657 RLD1 CS913Ei-2 CS22616 Rmx-A180 CS22569Est-1 CS22629 Sav-0 CS1514Fab-2 CS22576 Shakadara CS6180Fei-0 CS22645 Sq-1 CS22600Fr-2 CS6708 Ts-1 CS22647Got-7 CS22608 Tsu-1 CS22641Gu-0 CS22617 Uk-1 CS1574Kin-0 CS1272 Van-0 CS22627Knox-0 CS22566 Wa-1 CS22644Knox-18 CS22567 Wei-0 CS22622Kz-1 CS22606 Ws-0 CS22623Ler-1 CS22618 Zdr-1 CS22588

Supplemental Table 1. List of Arabidopsis accessions that did not produce the developmental arrest in response to DFPM treatment.

Supplemental Data. Kim et al. Plant Cell. (2012). 10.1105/tpc.112.107235

8

RIL Ler Col RIL Ler Col RIL Ler Col933 1932 196620 1933 1967

1900 1934 19681901 1935 19694656 1936 19701903 1937 19711904 1938 19721905 1939 19731906 1940 19741907 1941 19751908 1942 19761909 1943 19771910 1944 19781911 1945 19791912 1946 19801913 1947 19811914 1948 19821915 1949 19831916 1950 19841917 1951 19851918 1952 19861919 1953 19871920 1954 19881921 1955 19891922 1956 19901923 1957 19911924 1958 19921925 1959 19931926 1960 19941927 1961 19951928 1962 19961929 1963 19971930 1964 19981931 1965 1999

Supplemental Table 2. DFPM-induced root growth arrest VICTR phenotype in 100 Col X Ler Recombinant Inbred Lines. Check-marks depict if Col (DFPM sensitive) or Ler (DFPM resitant) root phenotype was documented in a specific RIL.

Supplemental Data. Kim et al. Plant Cell. (2012). 10.1105/tpc.112.107235

9

Supplemental Methods

Phylogenetic Analysis. The protein sequences encoded by representative gene

models for construction of the alignment and phylogenetic tree were obtained

from the TAIR website (TAIR 10). Prior to the analysis the sequence names were

removed, as they are incompatible with the character length limitations of the

PHYLIP format. The alignment was generated using MAFFT (v6.717b

(2009/12/03) (Katoh et al., 2002) and the L-INS-i alignment method. Further the

alignment was curated using rascal (version 1.34) (Thompson et al., 2003). The

phylogenetic analysis was performed using the parallel command line version of

PhyML (version 20120412) (Guindon and Gascuel, 2003) and the following

settings: data type: amino acid, model: WAG, bootstrap: 1000, amino acid

frequencies are estimated by the model (option -f m), topology search: SPR, five

random initial tree were used (option: --rand_start, --n_rand_starts 5). The

generated tree was visualized using FigTree (version 1.3.1;

http://tree.bio.ed.ac.uk/software/figtree/. The scale bar indicates 0.2 substitutions

per site.

Guindon, S., and Gascuel, O. (2003). A Simple, Fast, and Accurate Algorithm

to Estimate Large Phylogenies by Maximum Likelihood. Systematic

Biology 52, 696-704.

Katoh, K., Misawa, K., Kuma, K.i., and Miyata, T. (2002). MAFFT: a novel

method for rapid multiple sequence alignment based on fast Fourier

transform. Nucleic acids research 30, 3059-3066.

Thompson, J.D., Thierry, J.C., and Poch, O. (2003). RASCAL: rapid scanning

and correction of multiple sequence alignments. Bioinformatics 19, 1155-

1161.

Supplemental Data. Kim et al. Plant Cell. (2012). 10.1105/tpc.112.107235

10