2014 North American Chemical Residue Workshop - NACRWTradeWinds Island Grand, St. Pete Beach, FL | July 20-23, 2014 | Booth# 15 & 16Vendor Workshop | Tuesday, July, 22nd | Tarpon Room | 12:10 pm - 1:10 pmMy QuEChERS Extraction is going on a Diet!Presenter: Joan Stevens, Ph. D., Agilent TechnologiesAbstract: The benefits of QuEChERS are well documented making this technique very popular and easily executed in any laboratory using common equipment such as pipettes, balance, vortexer and centrifuge. Because of the benefits QuEChERS provides as a sample preparation technique, it is rapidly expanding to other matrices and compound classes. It is true that QuEChERS is a simple and easy technique. But, the QuEChERS procedure does not entirely remove the matrix, which can negatively affect your analysis and instrumentation. Lipids, for instance, are a very problematic component remaining in the QuEChERS extraction especially with high lipid content samples like avocadoes and oils. Dispersive-SPE and cartridge SPE containing C18, hydrophobic sorbent, are used to remove some of the lipids from the extract, but many times a large percentage of lipids remains. In this session, we will present alternative approaches for increasing lipid removal from QuEChERS extract.

Tools for Identifying Pesticide Metabolites in Fruits and Vegetables Using Accurate Mass LC/Q-TOF MSPresenter: Jerry Zweigenbaum, Ph. D., Agilent TechnologiesAbstract: This presentation will focus on the tools available in MassHunter Software that can be used to identify metabolites of pesticides in plant material. In the allotted time, and a workflow will be described using different acquisition modes. Data processing will include the use of metabolite ID (briefly), using a diagnostic ion with all ions and AutoMSMS, using AutoMSMS and neutral loss, a molecular formula generator filter, MassProfiler, and MassProfiler Professional with Profinder. The same tools can be used for the identification of metabolites of other classes of compounds and in other types of samples (e.g. urine or blood).

Maximum registration: 100 | You can register for the Agilent lunch-n-learn workshop when you check-in at NACRW

Agilent User Meeting | Thursday, July, 24th | Citrus Room | 10:30 am - 12:30 pmYou can register for the Agilent User Meeting at: www.agilent.com/chem/NACRWLC/QQQ Method Development from A to Z | Jerry Zweigenbaum, Ph. D., Agilent TechnologiesAbstract: This will be a workshop type examination of a thorough process for developing an LC/QQQ method with the Agilent 6400 Series Triple Quadrupole LC/MS. A workflow of using Agilent provided databases and adding to or building custom ones in the Agilent MRM optimizer will be described. Once constructed the information is transferred from the database to a custom acquisition method. Chromatographic method development follows and will be briefly described including the use of a 6 column selector valve to optimize stationary phase selectivity. Once robust chromatography is achieved, the method is completed by running the Agilent source optimizer. This presentation is for anyone using or interested in using the Agilent 6400 Series Triple Quadrupole LC/MS for ultra-trace analysis.

Automated Screening for Hundreds of Pesticide Residues using a GC/Q-TOF with a new Exact Mass Pesticide DatabasePhilip L. Wylie, Ph. D., Agilent TechnologiesAbstract: This discussion introduces new MassHunter data analysis software and an exact mass pesticide database/library containing more than 700 compounds. The Agilent 7200 high resolution accurate mass GC/Q-TOF is run in the TOF mode using a retention time locked method that matches the database retention times. For each compound, “all ions” software automates screening by choosing characteristic exact mass ions and extracting them from the chromatogram.

Validation of GC-MS/MS confirmatory method for the European official control of levels of dioxins, furans, and dioxin-like PCBs in foodstuffs | Benjamin L’Homme, University of Liège, BelgiumAbstract: This work aims to prove that this technology meets requirements and complies with standard criteria derived from those established for GC-HRMS and adapted to the specificity of tandem MS. The MS/MS method has been validated for feed material of plant origin (vegetable oil) at the regulation level of 0,75ng WHO-PCDD/F-TEQ/kg and 1,5ng WHO-PCDD/F-PCB-TEQ/kg. Results are also compared with the current routine confirmatory GC-HRMS method. Validation will further be extended to other matrices.

Sample Preparation: Method Development made Easy for Complex Matrices | Joan Stevens, Ph. D., Agilent TechnologiesAbstract: Are you faced with developing or improving your sample preparation methods for unusual matrices? If so, we will provide a simple, straight forward approach to modifications that will increase compound recovery from unique matrices focusing on QuEChERS and Solid Phase Extraction methodology.The benefits of QuEChERS are well documented, making this technique very popular and easily executed. Because of the benefits QuEChERS provides as a sample preparation technique, it is rapidly expanding to other matrices and compound classes. Therefore, simple modifications might be required to the basic procedure in order to optimize compound recovery from unique matrices.Maximum registration: 35

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Agilent 7010 Triple Quadrupole GC/MS with 7890B GC and 7693 ALS

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Agilent Science at FPRW 2014Determination of Illegal Dyes in Spices by QuEChERS and LC-MS/MS AnalysisJoan Stevens,1 Derick Lucas.1 | 1Agilent Technologies, Wilmington, DE 19808, USA

Optimizing Recoveries from Challenging Matrices through Unique Modifications to the QuEChERS Method Joan Stevens,1 Derick Lucas.1 | 1Agilent Technologies, Wilmington, DE 19808, USA

Screening for Hundreds of Pesticides by GC/Q-TOF: New Software with a New Exact Mass Library Philip L. Wylie1 and Sofia Aronova2 | 1Agilent Technologies, Wilmington, DE 19808, USA | 2Agilent Technologies, Santa Clara, CA USA

Monday, July 21, 2014 | Session 3, 4:00P - 4:20PQuality Control/Quality Assurance for Qualitative Analysis using High Resolution and Accurate Measurement Mass SpectrometryJerry Zweigenbaum | Agilent Technologies, Wilmington DE ,USA

Wednesday, July 23, 2014 | Session 8, 1:15P - 1:35PA New Paradigm in Environmental Health Sciences: Using the Exposome to Determine the Cause of Chronic Human DiseaseAnthony Macherone1,2 | 1Agilent Technologies, Wilmington DE, USA | 2John Hopkins School of Medicine, Baltimore MD, USA

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2014 North American Chemical Residue Workshop - NACRWTradeWinds Island Grand, St. Pete Beach, FL | July 20-23, 2014 | Booth# 15 & 16