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pENTR™ Directional TOPO® Cloning Kits1. TOPO ® Clone your blunt-end PCR product into one of the pENTR™ TOPO vectors to generate an entry clone. 2. Generate an expression construct
Supplementary Materials for...using the pENTR/D-TOPO kit and subsequent LR reaction following the protocol (Invitrogen). For GUS staining assay, 2 kb promoters of BUPS1, BUPS2, RALF4,
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Gateway pENTR Dual Selection Vectors - Thermo … Information pENTR Dual Selection Vectors This manual is supplied with the following products. Product Catalog no. pENTR 1A Dual Selection
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pENTR™ Directional TOPO® Cloning Kitslabs.fccc.edu/yen/docs/pentr_dtopo_man.pdf · pENTR™ Directional TOPO® Cloning Kits Five-minute, directional TOPO® Cloning of blunt-end
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Gateway™ pENTR™ Vectorsyrgene.com/documents/vector/pentr.pdfbiochemistry, refer to Molecular Cloning: A Laboratory Manual (Sambrook et al., 1989) or Current Protocols in Molecular
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Gateway pENTR Dual Selection Vectors...Vectors A large selection of Gateway® destination vectors is available from Invitrogen to facilitate expression of your gene of interest in
Supplementary Materials for · For LR recombination reactions: 1 µL of purified pENTR harboring a deletion cassette was incubated with 1 µL of the destination vector, pNPTS-SPEC-DEST,
