26

1.

11)

)

2.

2.1

25

100

2.2

DA-645

pH

)

GCMS-QP2010

1 24

2.3

45

26 26

32

37

4 1

7

6

3.

2.1

6

7

72.4ppm

- 39.8ppm

35.9ppm 2.1ppm

106.9ppm 3.2ppm

211ppm

79.1ppm 94.7ppm

276ppm 318ppm, 416ppm

16ppm

6 7

13

-0.548** -0.458**

r=0.000

0.382**

0.443**

VIP (Variable Importance for Projection)

VIP

4.

.

1)

2)

26

A

(C)

E

B n-

D

F

0

5

10

15

20

25Ethyl  Acetate  Max 143.0 ppmMin. 24.3 ppmAve. 72.4 ppmSD 22.4 ppm

0

5

10

15

20

25

isobuthanol  Max  65.3  ppmMin.  15.7  ppmAve.  35.9  ppmSD  10.2        ppm

0

5

10

15

20

25

30isoamyl  alcholMax 187.6  ppmMin. 74.3 ppmAve. 106.9 ppmSD 17.1 ppm

0

5

10

15

20

25n-‐PropanolMax 74.4 ppmMin. 20.1 ppmAve. 39.8 ppmSD 12.8 ppm

0

5

10

15

20

25isoamyl  acetate  Max  9.2  ppmMin.  0.7  ppmAve.  2.1  ppmSD  1.4      ppm

0

5

10

15

20

25

30

35ethyl  caprate  Max  13.4  ppm  Min.  0.4    ppmAve.  3.2  ppmSD  2.6        ppm

n-

26 26

A

C

E

G

B

D

F

0

2

4

6

8

10

12

14

16phosphoric  acid  Max  322.1  ppmMin.  133.4  ppmAve.  211.7  ppmSD  37.5            ppm  

0

5

10

15

20

25

30

35

40

45

50pyruvic  acidMax 332.0  ppmMin. 9.7 ppmAve. 94.7 ppmSD 82.5            ppm

0

5

10

15

20

25succinic    acidMax 464.9 ppmMin. 200.0  ppmAve. 318.2 ppmSD 56.5 ppm

0

5

10

15

20

25

30

35acetic  acidMax 55.8 ppmMin. 2.8 ppmAve. 16.0 ppmSD 10.3        ppm

0

5

10

15

20

25

30citric  acid  Max  127.6  ppmMin.  44.0      ppmAve.  79.1      ppmSD  17.8          ppm

0

2

4

6

8

10

12

14

16malic    acidMax 686.2  ppmMin. 116.2  ppmAve. 275.8  ppmSD 71.4 ppm

0

5

10

15

20

25

30

35lactic  acid  Max 761.3  ppmMin. 269.6 ppmAve. 416.0  ppmSD 83.4            ppm

26

4 VIP

1)

25

https://www.nta.go.jp/shiraberu/senmonjoho

/sake/shiori-gaikyo/seibun/06.htm

2) 100 9

639-649 2005

3)

104 2 131-141 (2009)

5 VIP

4) Yukiko Maeda, Masaki Okuda, Katsumi Hashizume,

Midori Joyo, Shigeaki Mikami, Nami

Goto-Yamamoto J Biosci Bioeng 112(3),

238-246 (2011)

5) Natsuki Mimura, Atsuko Isogai, Kazuhiro

Iwashita, Takeshi Bamba, Eiichiro Fukusaki,

J Biosci Bioeng, 118(4) , 406-414 (2014)

6)

1990

7)

186

2014 p -16

0.00

0.10

0.20

0.30

0.40

0.50

0.60

0.70

0.80

0.90

1.00

()

4VG  …

0.00

0.10

0.20

0.30

0.40

0.50

0.60

0.70

0.80

0.90

1.00

Ethyl  Acetate n-‐Propanol Isobuthanol Isoamylacetate

Isoamylalchol

Ethyl  caprate Phosphoricacid

Citric  acid Pyruvic  acid Malic  acid Succinic  acid Lactic  acid Acetic acid