2nd ICB-Pharma ProgrammeAndAbstractBook

7/24/2019 2nd ICB-Pharma ProgrammeAndAbstractBook

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INTERNATIONAL CONFERENCE

ICB PHARMA II

PROGRAMME

andABSTRACT BOOK

Current Breakthrough in

Pharmacy Materials and Analyses

Faculty of Pharmacy

Universitas Muhammadiyah Surakarta

2015


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Current Breakthrough in Pharmacy Materials and Analyses

Pharmacy Faculty, Universitas Muhammadiyah Surakarta

Surakarta, Indonesia

Chairman of Editors : Erindyah Retno Wikantyasning, Ph.D., Apt.

Tanti Azizah Sujono, M.Sc., Apt.

Team of Pharmaceutical Technology : Anita Sukmawati, Ph.D., Apt.

Suprapto, M.Sc.,Apt.Erindyah Retno Wikantyasning, Ph.D., Apt.

Gunawan Setiyadi, M.Sc., Apt.

Team of Pharmacology and Microbiology : Azis, Saifudin, Ph.D., Apt.

Arifah Sri Wahyuni, M.Sc., Apt.

Ratna Yuliani, M.Biotech.St.

Tanti Azizah Sujono, M.Sc., Apt.

Team of Clinical and Community Pharmacy : Dra. Nurul Mutmainah, M.Si., Apt.

Zakky Cholisoh, M.Clin. Pharm., Ph.D., Apt.Hidayah Karuniawati, M.Sc. Apt.

Team of Pharmaceutical Chemistry : Dedi Hanwar, M.Si., Apt.

Dr. Muhammad Dai, M.Si., Apt.

Dr. Muhtadi Ibrahim, M.Sc.Broto Santoso, M.Sc., Apt.

Andi Suhendi, M.Sc., Apt.

Ika Trisharyanti, M.Sc., Apt.

Team of Molecular Biology : Agus Purnomohadi, M.Sc.

Maryati, Ph.D., Apt.

Copyright 2015

Copyright in compilers and reserved

Design cover: Publication and DocumentationTeam Layout: Secretaryand IT Team

Published by:

Muhammadiyah University PressUniversitas Muhammadiyah Surakarta

Jl. A. Yani Pabelan Tromol Pos I Kartasura Surakarta 57102Telp. (+62 271) 717417-172, E-mail: [email protected]


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ICB Pharma II 31 October 2015

Current Breakthrough in Pharmacy Materials and Analysesiii | P a g e

PREFACE

Its my great pleasure to welcome you to the 2nd International Current

Breakthrough (ICB)-Pharma Symposium 2015 in Solo Indonesia which will be held on 31

October, 2015 under the auspices of Universitas Muhammadiyah Surakarta.

ICB-Pharma 2015 will feature a theme of Current Breakthrough in Pharmacy

Material and Analyses and will consist of morning and afternoon sessions. There will be

plenary lectures and session lectures given by several invited speakers from Singapore,

Japan, as well as from Indonesia, and also selected oral presentations of the submitted

papers. The poster session from various fields of pharmaceutical sciences will take place

nearby.

The ICB-Pharma will be directed for a tradition and in the future will be nurtured

as a well-known scientific symposium in disseminating the breakthrough and novel

technology in pharmacy materials. This purpose will be able to achieve by encouragement

of national and international academic institution partners and supports from the

industrial partners, Indonesian Pharmacist Association (Ikatan Apoteker Indonesia, IAI),

colleagues and from the organizing committee. Moreover, I do hope and believe that, the

2nd ICB-Pharma will offer great opportunities for the scientists to meet and discuss recent

topics in the field of material and pharmaceutical science and bring the academics, health

professionals and industries together for sharing their experiences to solve current

problems and challenges in practice.

Warm regards,

Azis Saifudin, PhD, Apt.

Chair of ICB-Pharma Symposium


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PREFACE

This conference is held to disseminate current methods which provide advanced

materials and methods in pharmacy. This is a good media for those who are engaged in

academic, industrial, regulatory fields to conduct social interactions, to share their

findings, to communicate bottle neck surrogates, and to seek the possibilities for

collaborations. We are quite humble to recognize our weakness in running all agendas.

Hence, from bottom of our heart we ask apologizes from all participants for any service,

lack facilities, low response, etc. However, we must be persistent and ensuring our

positive contribution toward scientific society especially at the attempt to develop

capacity building of pharmaceutical sciences in Indonesia. Thus, we will run ICB Pharmacy

III next year.

To all participants, I wish an inspiring moment in Solo city, a city where was born a

national leader and city of heritage!

Azis Saifudin, PhD, Apt.

Dean of Faculty of Pharmacy

Universitas Muhammadiyah Surakarta


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TABLE OF CONTENTS

PREFACE .................................................................................................................................................................. iii

TABLE OF CONTENTS .......................................................................................................................................... vPROGRAMME .......................................................................................................................................................... ix

ORAL PRESENTER ROOM................................................................................................................................... x

LIST OF PARTICIPANT NON PRESENTERS ...................... ..................... ..................... ...................... .......... xi

LIST OF ORAL PRESENTERS ......................................................................................................................... xvi

LIST OF POSTER PRESENTERS ................... ...................... ...................... ...................... ..................... ........ xviii

SPEAKER ................................................................................................................................................................... 1

A-PHARMACEUTICAL TECHNOLOGY ............................................................................................. 4

A001 CHARACTERISTICS TESTING OF MICROCRYSTALLINE CELLULOSE FROM NATA

DE COCO COMPARED TO AVICEL pH 101 AND AVICEL pH 102

Adi Yugatama1*, Laksmi Maharani2, Hening Pratiwi2, Lingga Ikaditya3................................ .......... 4

A002 PREPARATION OF ARTIFICIAL SALIVA FORMULATION

Andi Sri Suriati Amal1*, Samsinah Hj. Hussain2, Mohd. Amin Jalaluddin3....................................... 5

A003 FORMULATION OF ETHANOL EXTRACT OF TUNJUK LANGIT (Helminthostachys

zaylanica) RHIZOME AS TABLET DOSAGE FORM BY WET GRANULATION

METHOD

Fitrya1*, Najma Annuria Fithri1, Budi Untari1, Aprililianti1..................... ..................... ...................... ... 6

A004 EFFECT COGRINDING ON THE PHYSICAL CHARACTERISTIC OF BINARY

MIXTURE OF PIOGLITAZONE HCl AND METFORMIN HCl

Iskandar Zulkarnain1*, S. N. Soewandhi1, S. Wikarsa2.................... ...................... ...................... ............. 7

A005 PREPARATION AND CHARACTERIZATION OF SUBMICRON PARTICLES OF PLGA

INCORPORATING RIFAMPIN USING EMULSION SOLVENT DIFFUSION METHOD

Mardiyanto1* ............................................................................................................................................................ 8

A006 LIQUID BATH SOAP FORMULATION AND ANTIBACTERIAL ACTIVITY TESTAGAINST Staphylococcus aureus OF KECOMBRANG (Etlingera elatior (Jack)

R.M.Sm.) FLOS EXTRACTS

Lilis Handrayani1, Ratih Aryani1*, Indra1................... ...................... ...................... ..................... .................. 9

A007 THE INFLUENCE OF EGGPLANT PEEL EXTRACT (Solanum melongena L.) ASANTIOXIDANT IN LOTION DOSAGE FORM

Sholichah Rohmani1* ......................................................................................................................................... 10

A008 OPTIMIZING COMBINATION OF SAMBILOTO HERBAL WATER FRACTION AND

SALAM LEAF WATER FRACTION AS ANTI-INFLAMMATION

Raymond Harris Mustafa1, Lannie Hadisoewignyo1*, Martha Ervina1, Lisa Soegianto1,Wahyu Dewi Tamayanti1 .............................................................................................................. 11


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B-PHARMACOLOGY AND MICROBIOLOGY ................................................................................. 12

B001 THE POTENTIAL OF THE BLACK RICE BRAN EXTRACT AS ANTIDIABETIC AGENT

Arifah Sri Wahyuni1*, Rima Munawwaroh1, Esthi Utaminingsih1, Novita Sari1.................... .... 12

B002 ANTIOXIDANT ACTIVITIES OF NANOEMULSION CONTAINING EXTRACT OFSAMBILOTO (Andrographis paniculata (Burm F.) Ness.) AND MENIRAN(Phyllanthus niruriL.) IN ALLOXAN- INDUCED DIABETIC RATS

Erindyah R Wikantyasning1*, Nurcahyanti Wahyuningtyas1, Muhammad Dai1, Doni

Wibowo1............................................................................................................................................... 13

B003 ACUTE TOXICITY STUDY OF NANOEMULSION CONTAINING SAMBILOTO

(Andrographis paniculata) LEAVES AND MENIRAN (Phyllanthus niruri) HERBS

EXTRACT IN WISTAR RATS

Erindyah R. Wikantyasning1*, Nurcahyanti Wahyuningtyas1, Muhammad Dai1, Febby

Lovita Sari1 .......................................................................................................................................... 14

B004 ANTI-INFLAMMATORY ACTIVITY TEST OF CHRISTMAS PALM (Adonidia merrillii(Becc.) Becc.) SEED EXTRACT IN MALE WISTAR RATS (Rattus norvegicus)

Herlina1*, Fitrya1, Fithri Najma A1, Rahmawati Dwi Shafarina1................... ..................... ............... 15

B005 THE EFFECT OF GIVING GLUCOMANNAN PORANG TUBER (Amorphophallus

oncophyllus Prain ex Hook. F.) ON SGPT AND SGOT LEVELS OF MALE WISTAR

RATS BLOOD INDUCED BY PARACETAMOL

Intan Martha Cahyani1*, Bekti Nugraheni1............................................................................................... 16

B006 THE PROFILE OF MDA (malondialdehyde) LEVEL IN RATS GIVEN EXTRACT OF

THYMI HERBS (Thymus vulgaris[L.])

Ken Inayati Latifa1*, Tanti Azizah Sujono1, Ika T. Dian Kusumowati1............................. .............. 17

B007 PRE-CLINICALSTUDY OF Cr (III) BASED HYPOGLICEMIC SUPPLEMENT IN-TYPE

2 DIABETIC RATS

Kun Sri Budiasih1*, Kartika Ratna Pertiwi1............................................................................................... 18

B008 SCREENING ANTIBACTERIAL POTENCY OF ENDOPHYTIC FUNGI METABOLITEOF MANGOSTEEN (GarciniamangostanaL.) LEAF

Lisa Soegianto1*, Martha Ervina1, Kevin Widjaja1, Angela Violita1............................... .................. 19

B009 ACTIVITIES OF THE COMBINED EXTRACTS OF TEMPUYUNG (Sonchus arvensis)

AND BLACK CUMIN (NIGELLA SATIVA) AGAINST XANTHINE OXIDASE

INHIBITION ON HYPERURICEMIC MICE

Muhtadi1*, Nurcahyanti Wahyuningtyas1, Andi Suhendi1, Septi Heryani1....................... ........... 20

B010 ANTIBACTERIAL ACTIVITY OF COMBINATION OF CHLORAMPHENICOL ANDETHANOLIC EXTRACT OF PACAR AIR (Impatiens balsamina) LEAVES AGAINST

Escherichia coliAND Shigella sonnei

Ratna Yuliani1*, Agung Cokro Prabowo1, Yeni Maisyah1..................................................................... 21

B011 ANTIHYPERCHOLESTEROLEMIC EFFECT OF Murbei (Morus alba L.) LEAVESAND ITS COMBINATION WITH SIMVASTATIN IN RATS INDUCED BY

PROPYLTIOURACIL AND HIGH FAT DIET

Tanti Azizah Sujono1*, Haryoto1, Ratna Kartikasari1, Laily Ieda Quntari1............................... .... 22


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B012 ANALGESIC EFFECT OF ETHANOLIC EXTRACT RED DRAGON FRUIT ( Hylocereus

polyrhizus Cortex) PEEL ON MALE MICE SWISS WEBSTER WITH WRITHING

REFLEX METHOD

Westi Fajrin Bayu Nugrahaini1*, Tanti Azizah Sujono1..................... ...................... ..................... ........ 23

B013 EFFECTS OF TURMERIC ETHANOLIC EXTRACT ON TRIMETHYLTIN-INDUCEDOXIDATIVE STRESS ON SPRAGUEY DAWLEY RATS

Sapto Yuliani1*, Mustofa Mustofa2, Ginus Partadiredja2................... ...................... ...................... ....... 24

B014 ANTIDIABETIC ACTIVITY OF NANOEMULSION CONTAINING EXTRACT OFSAMBILOTO (Andrographis paniculata (BURM F.) NESS.) AND MENIRAN

(Phyllanthus niruriL.) IN ALLOXAN-INDUCED DIABETIC RATS ................... ............... 25

Erindyah R. Wikantyasning1*, Nurcahyanti Wahyuningtyas1, Muhammad Dai1, FajarKholikul Amri1 ................................................................................................................................... 25

C-CLINICAL AND COMMUNITY PHARMACY .............................................................................. 26

C001 OVERVIEW ABOUT ANTIBIOTICS PRESCRIBING IN URINARY TRACT INFECTIONROEMANI SEMARANG HOSPITALS INPATIENTS

Hening Pratiwi1* .................................................................................................................................................. 26

C002 RATIONALITY TREATMENT OF ANTIBIOTICS FOR TREATMENT OF DIARRHEA

IN ADULT PATIENTS IN THE INPATIENT INSTALLATION OF HOSPITAL XSURAKARTA IN 2014

Ida Ayu Pebrina1*, Suharsono1, Suprapto1................................................................................................ 27

C003 CLINICAL IMPROVEMENT AFTER CEPHALOSPORINE THERAPY ON CHILDREN

WITH TYPHOID FEVER

Rasmaya Niruri1*, N.P. Yulia Purnami1, Julius D.Tansale2,3, I.B.Eka Erlangga3...................... .... 28

C004 TRANSAMINITIS ASSOCIATED WITH HIGH DOSE METHOTREXATE AND 6-

MERCAPTOPURIN IN CHILDREN WITH ACUTE LYMPHOBLASTIC LEUKEMIA

Rasmaya Niruri1*, K. Trisna Komalasari1, Ketut Ariawati2................................................................ 29

C005 TREATMENT AND COST ANALYSIS OF DIARRHEA PATIENTS OF INPATIENTINSTALLATION OF RSUD dr. MOEWARDI SURAKARTA BY BPJS PROGRAM IN

2014

Saktya Ayu Donna P1*, Suharsono1, Suprapto1........................................................................................ 30

D-PHARMACEUTICAL CHEMISTRY ............................................................................................... 31

D001 ANTIOXIDANT ACTIVITY OF ETHYL ACETATE EXTRACT OF PARKIA SEED AND

POD (Parkia speciosa Hassk.) BY DPPH (1,1-DIPHENYL-2- PICRYLHYDRAZYL)METHOD .............................................................................................................................................. 31

Indri Kusuma Dewi1*, Agus Winarso1......................................................................................................... 31

D002 A CLICK-TYPE COUPLING REACTION BETWEEN THIOAMIDES AND SULFONYL

AZIDES AS A VERSATILE APPROACH TO GENERATE NEWPHARMACOLOGICALLY ACTIVE COMPOUNDS .................... ..................... ...................... .... 32

Muhammad Aswad1, Junya Chiba1*,Yasumaru Hatanaka1, Takenori Tomohiro1..................... 32

D003 ANTIBACTERIAL COMPOUND PRODUCED BY A SOIL BACTERIA ISOLATED FROMRIZHOSPHERE OFZingiber officinale....................................................................................... 33


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Nanik Sulistyani1,2*, Yosi Bayu Murti3, Jaka Widada4, Mustofa5................... ..................... ............... 33

E-MOLECULAR BIOLOGY .................................................................................................................. 34

E001 CYTOTOXIC ACTIVITY OF POLAR, SEMIPOLAR, AND NON POLAR FRACTION OF

ETHANOL EXTRACT OF SALA PLANTS LEAVES (Cynometra ramiflora Linn.)

AGAINTS WiDr CELL

Haryoto1*, Anis N. Irjayanti1, Tanti Azizah Sujono1, Muhtadi1, Andi Suhendi1.................. ........ 34

E002 CHALCONES DERIVATIVE WITH BROMO SUBSTITUENT INDUCES APOPTOSIS IN

HeLa CELLS

Retno Arianingrum1*, Indyah Sulistyo Arty1.................... ..................... ...................... ..................... ........ 35

E003 SUB-CLONING OF ads GENE INTO pETDUET1_cyp FOR CO-EXPRESSION IN

ESCHERICHIA COLI

Imam A. Wicaksono1, Tresna Lestari2*, Evi U. Ulfa3, Catur Riyani1, Elfahmi1............................. 36

CV OF SPEAKER ................................................................................................................................................... 37

LIST OF COMMITTEES ...................................................................................................................................... 40

MAP OF FACULTY OF PHARMACY .................... ...................... ...................... ...................... ..................... .... 42

LIST OF TAXI AND HOTELS ............................................................................................................................ 43


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PROGRAMME

Venue:Auditorium Moh. Djazman, Universitas Muhammadiyah Surakarta

Jl. Ahmad Yani, Tromol Pos I, Pabelan, Surakarta 57162 Indonesia

Session:Morning Session

07.00-08.00 Registration for participant

08.00-08.30 Opening Ceremony

08.30-09.00 Morning Coffee Break

09.00-09.45 Speaker 1

Prof. Jackie Ying (Executive Director, Institute of Bio-engineering and

Nanotechnology)Topic: "Current status on nano medicine and nano devices"

09.45-10.30 Speaker 2

Assoc. Prof. Takenori Tomohiro (University of Toyama, Japan).Topic: "Photoaffinity labeling-based target identification of bioactive

molecules"

10.30-11-15 Speaker 3

Dr. Wangsa Tirta Ismaya (Scientist/Head section of Recombinant

therapeutic proteins, PT. Dexa Medica).

Topic: "Current status of recombinant therapeutic proteins and localissues related"

11.15-12.00 Discussion12.00-13.00 Lunch break

Afternoon Session

13.00-13.30 Poster Session

13.30-16.00 Oral Presentation Session

16.00-16.30 Closing Ceremony


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ORAL PRESENTER ROOM

Room Code Presenter Moderator

K4-A

A001 ADI YUGATAMA

Gunawan Setiyadi, M.Sc.,Apt.

A003 FITRYA

A005 MARDIYANTO

A006 RATIH ARYANI

A008

K4-B

C001 HENING PRATIWI

Andi Suhendi, MSc., Apt.C003 RASMAYA NIRURI

D001 INDRI KUSUMA DEWI

D002 MUHAMMAD ASWAD

K4-C

B001 ARIFAH WAHYUNI

Dedi Hanwar, M.Si., Apt.

B004 HERLINA

B007 KUN BUDIASIH

B009 MUHTADI

B011 TANTI AZIZAH

K4-D

B008 LISA SOEGIANTO

Maryati, Ph.D., Apt.

B010 RATNA YULIANI

E001 HARYOTO

E002 RETNO ARIANINGRUM

E003 TRESNA LESTARI


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LIST OF PARTICIPANT NON PRESENTERS

No Name Institution

1 ARDIANSAH UMS2 HIMYATUL HIDAYAH PRODI MAGISTER FARMASI UMS

3 MARIA ULFA PRODI MAGISTER FARMASI UMS

4 MARIA ULFA ANANDA FARMA

5 ABDUL RONI UMS

6 MUSTAKIM MASNUR UMS

7 DITA MARINA LUPITANINGRUM PRODI MAGISTER FARMASI UMS

8 UMI KHOLIFAH.S.FARM.,APT APOTEK KARUNIA FARMA

9 LENI HERLINA WIDIASARI, S.SI.,APT. APOTEK WALI SEHAT SEMARANG

10DHIGNA LUTHFIYANI CITRA

PRADANAUNIVERSITAS JAMBI

11 YUSNITA APRILIYANA APOTEK SIDAPURNA

12 NOVI DIANASARI, S.FARM, APT. APOTIK KONDANG WARAS

13DEVI UTAMI YULISTYANTI S. FARM.,

APTELLA SKIN CARE SOLO

14 DIKA HERNAWATI S.FARM., APT ELLA SKIN CARE SOLO

15 ARIS SETIAWAN KLINIK INSANI MEDICA

16 UNGKI PRASETYO KLINIK RETNO

17MARIA RINA DWI

SUSILOWATI.,S.SI.,APTPT. GRAHA FARMA

18 DRA. DWI MURTINGHASTUTI,APT PT. GRAHA FARMA

19AGNES RINA SRI MURWANI,

S.SI.,APTPT. GRAHA FARMA

20 DWI KARTIKA SANTI RS PKU MUHAMMADIYA KARTASURA

21 SRI HARTINI, S.FARM.APT APOTEK SUMBER SEHAT BOYOLALI

22NUR SEKTI HIDAYAT RAHMAWATI

S. FARM., APT.UMS

23DIAZ VEGA AKHIRUNNISA, S.FARM.,

APTLBC WONOSOBO

24 ANNISA RIZKI MURDIYANI PT. DAYA MUDA AGUNG

25 UMI KHOLIFAH.S.FARM.,APT APOTEK KARUNIA FARMA

26 HANDIKA DESI YANOTAMA SMK ASSHODIQIYAH SEMARANG27 RETNO HARI WAHYUNI BPOM RI

28CAESNAN MARENDRA GRAHAN

LEDITTOUNIVERSITAS KRISTEN IMMANUEL

29 SARAH PUSPITA ATMAJA AIRLANGGA UNIVERSITY

30 MEILANA SUSANTI APOTEK HARJOSARI FARMA

31 ULFAHTUL LAELI APOTEK DAWA FARMA

32 INAYATUL HIDAYATI APOTEK SERAYA

33 LULU BACHRIYAH APOTEK MUTIARA


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No Name Institution

34 MARLIN TIGOR S.SI. APT APOTEK PUTRID

35 LENI HERLINA WIDIASARI SSI.,APT APOTIK WALI SEHAT

36

ARRYSKA AYU PERMATASARI

S.FARM.,APT IAI KAB TEGAL37 MALICHATUN APOTEK KAROMAH

38 RIZAMDHANI APOTEK KIRANI

39 LINNA MARLINNA S.SI, APT IAI PC KAB. TEGAL

40HENDRA ANTAN DJAYA, S.SI, MBA,

APTIAI PC KAB. TEGAL

41 QUROTUL AINI, S.FARM.,APT APOTEK MITRA MEDIKA

42 IRA RAHMIYANISTIKES BAKTI TUNAS HUSADA

TASIKMALAYA

43FRANSISCA MEINDRIA NARASTY,

S.FARM., APT.PT. GRAHA FARMA SURAKARTA

44 DESTANTIA NADYA AMANTHAS.FARM., APT

STIFAR YAYASAN PHARMASISEMARANG

45 TYAS SARININGRUM S.FARM., APTSTIFAR YAYASAN PHARMASI

SEMARANG

46 CANDRA EKA PUSPITASARI UNIVERSITAS GADJAH MADA

47DHIGNA LUTHFIYANI CITRA

PRADANAUNIVERSITAS JAMBI

48 FARIDHA CYNTHIA DHEWANTI UMS

49 SITI CHOTIAH UMS

50 AMIRA UMS

51 TRI YOGO WIBOWO UMS

52 AHMAD RIFQI KURNIA UMS

53 ABDUL HADI UMS

54 NUR ALFIAWATI UMS

55 CHOIRUL MA'ARIF UMS

56 RAFA ENBUN RELIGIA UMS

57 SOVY SAPTA NUARI PRAMOLIS UMS

58 NAUFAL SATRIA HUTOMO UMS

59 HESTU PUTRI MITAYANI UMS

60 SANGGITA AYU IKASARI UMS

61 FAHMI AZHARI UMS

62 DYAH RISWARI PITALOKA UMS

63 ALIFAH ANASTYA DINI UMS

64 RANI UTAMI WIDYANINGRUM UMS

65 MARHAMAH NUR AZIZAH UMS

66 DRAJAT TRI WAHYUDI UMS

67 LITA RAHIMA OENSJAR UMS

68 EKHWAN TRIS WANTO UMS

69ANDHIKA RIZKY GILANG

MAHAPUTRAUMS

70 ULITYATINING TIASARI UMS


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No Name Institution

71 AMALIYAH DINA ANGGRAENI UMS

72 WIWIK NURYANI UMS

73 AGUNG COKRO PRABOWO UMS

74 ESTUNINGTYAS AYU HAPSARI UMS

75 RISQIE CAHYANING KUSUMASARI UMS

76 IRHAMADI MALIK UMS

77 YENI MAISYAH UMS

78 UMUL BAROROTUY SYAMS UMS

79 UMI NURHAYATI UMS

80 RIZKI OCTAVIANA UMS

81 BAIQ SUPRAMONIKA UMS

82 MUTMAINNAH UMS

83 HAZRINI TANJUNG SARI UMS84 RENY WIDYA ESTUTI UMS

85 ABULKHAIR ABDULLAH UMS

86 SHIFA SILFIA UMS

87 NURYATI KUMAN UMS

88 FITRIANISA FATHUROHMAH UMS

89 IMROATUL CHASANAH UMS

90 MARWIANI ARUM SARI UMS

91 RAHAYU DWI KURNIAWATI UMS

92 ALISA PRIHARSI UMS

93 JANIKA SUJI KUSUMAWARDANI UMS

94 AYU ANGGRAENY UMS

95 EMAMATUL KHUTSIYAH UMS

96 BUNGA INTAN SAVITRI UMS

97 ANNIE RAHMATILLAH UMS

98 ISTIQAMATUSH SHOLIHAH UMS

99 ANGGRIANA ARISTYA UMS

100 ISNAINI NUR HIDAYAH UMS

101 NAHYATU SUFIAH UMS

102 RAUDAH PUTERI ALMINA UMS103 ADIVA TANTYAS AURORA UMS

104 FADILA KHOIRUNNISA UMS

105 SARI WIJAYANTI UMS

106 INTAN RIA NURJANAH UMS

107 SITI PURWATI UMS

108 SUNJAWA ALIF SAPUTRI UMS

109 KURNIA PUNGKY ASMORO UMS

110 NIKEN DWI MULYASARI UMS


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No Name Institution

111 AISYAH PUTRIANI UMS

112 FADHILA DIAH SUMINAR UMS

113 SEKAR PUJI UTAMI UMS

114 WITRI DYAH MEILANI UMS

115 NINA NURMITA HABSARI UMS

116 VERANTIKA DEA INDRASWARI UMS

117 TRI AGUS SAROSO UMS

118 YUDA MARSONO UMS

119 AULIA ANNUR AISYIAH UMS

120 ADHI WARDHANA AMRULLAH UMS

121 FEBRIANNA SURYANINGTYAS UMS

122 ANINDYA SETYOWATI UMS

123 NOVITA SARI UMS124 RATNA KARTIKASARI UMS

125 ESTHI UTAMININGSIH UMS

126 YENY DWI NOVITASARI UMS

127 NENI LUGKI NIAN TARY UMS

128 SALLY ASTYA UTAMI UMS

129 TANTIN HAYU SURYA UMS

130 THORIQ MAHMUD UMS

131 RAKIH YUSMA RANGGA UMS

132 HENDRA YUANA UMS

133 GITA AYU PRADINA UMS

134 BERNADI WICAKSONO UMS

135 ROSMA FAUZIAH UMS

136 TESAR ZULMI ANTORO UMS

137 ISTI SETIA HAPSARI UMS

138 BENY DWI HATMOKO UMS

139 FIKA RIZQIYANA UMS

140 ANGGITA SEKAR ARUMSASI UMS

141 DIAN YULISTIA ASTRI UMS

142 DIAN AYU ARA ARA ARTHASARI UMS143 EKA PRASNAPARAMITA W UMS

144 RIZKA ASTIKAH FITRIANA UMS

145 NUNGKY ASMARANING WAHYONO UMS

146 DEVI AMBARRINI WAHYUNINGTYAS UMS

147 DESTY RIRIN ROHMAWATI UMS

148 EKA PRADITA PUTRI UMS

149 AKHMAD AMINUR RIZKI UMS

150 LAILY IEDA QUNTARI UMS

151 MUHAMMAD PRIYADI UMS


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No Name Institution

152 MANGGAR ARUM SHINTYA M. UMS

153 DWI SETIANINGRUM MAKUNTI UMS

154 TITIS MUTALIKAH UMS

155 MARIA ULFA UMS

156 EKA SETIANISA UMS

157 ORLAN PAKAMBANAN UMS

158 APRILYA SRI RACHMAYANTI UMS

159 DIAN RAHMAWATI UMS

160 DWI SARYANTIAKADEMI FARMASI NASIONAL

SURAKARTA

161 SALSABIELA DWIYUDRISA SUYUDI UMS

162 NORA FAUZIAH UMS

163 RINI YULIANA UMS

164 LILIS FITRIATUN NISA UMS

165 EDI SUTARMANTO PRODI MAGISTER FARMASI UMS

166 NURLELA IKAWATI PRODI MAGISTER FARMASI UMS

167 INES NURFITRIANA PRODI MAGISTER FARMASI UMS

168 WULAN PRIATIWI PRODI MAGISTER FARMASI UMS

169KATHLEEN APRIANA

KRISTININGRUM JAHAMOUUMS

170 YULI FITRIANA PRODI MAGISTER FARMASI UMS

171 HALIDA SURYADINI UMS

172 PUTRI RAMDANIAH PRODI MAGISTER FARMASI UMS

173 CHANDRA EKA PUSPITASARI UGM


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xvi | P a g e ICB Pharma II 31 October 2015


LIST OF ORAL PRESENTERS

No Code Presenter Institution Title

1 A001 ADI YUGATAMA UNIV.

SEBELASMARET

CHARACTERISTICS TESTING OF

MICROCRYSTALLINE CELLULOSE FROMNATA DE COCO COMPARED TO AVICEL pH

101 AND AVICEL pH 102

2 A003 FITRYA SRIWIJAYA

UNIVERSITY

FORMULATION OF ETHANOL EXTRACT OF

TUNJUK LANGIT (Helminthostachys

zaylanica) RHIZOME AS TABLET DOSAGE

FORM BY WET GRANULATION METHOD

3 A005 MARDIYANTO SRIWIJAYA

UNIVERSITY

PREPARATION AND CHARACTERIZATION OF

SUBMICRON PARTICLES OF PLGA

INCORPORATING RIFAMPIN USING

EMULSION SOLVENT DIFFUSION METHOD

4 A006 RATIH ARYANI STIKES BAKTI

TUNAS

HUSADA

LIQUID BATH SOAP FORMULATION AND

ANTIBACTERIAL ACTIVITY TEST AGAINST

Staphylococcus aureus OF KECOMBRANG(Etlingera elatior(Jack) R.M.Sm.) FLOS

EXTRACTS

5 A008 RAYMOND

HARRIS

MUSTAFA

WIDYA

MANDALA

SURABAYA

CATHOLIC

UNIVERSITY

OPTIMIZING COMBINATION OF SAMBILOTO

HERBAL WATER FRACTION AND SALAM

LEAF WATER FRACTION AS ANTI-

INFLAMMATION

6 B001 ARIFAH

WAHYUNI

UMS THE POTENTIAL OF THE BLACK RICE BRAN

EXTRACT AS ANTIDIABETIC AGENT

7 B004 HERLINA SRIWIJAYA

UNIVERSITY

ANTI-INFLAMMATORY ACTIVITY TEST OF

CHRISTMAS PALM (Adonidia merrillii(Becc.)

Becc.) SEED EXTRACT IN MALE WISTAR

RATS (Rattus norvegicus)

8 B007 KUN BUDIASIH UNY PRE-CLINICAL STUDY OF CR (III) BASED

HYPOGLICEMIC SUPPLEMENT IN TYPE 2

DIABETIC RATS

9 B008 LISA SOEGIANTO WIDYA

MANDALA

SURABAYA

CATHOLIC

UNIVERSITY

SCREENING ANTIBACTERIAL POTENCY OF

METABOLITE ENDOPHYTIC FUNGI

MANGOSTEEN (Garcinia mangostanaL.)

LEAF

10 B009 MUHTADI UMS ACTIVITIES OF THE COMBINED EXTRACTS

OF TEMPUYUNG (Sonchus arvensis)AND

BLACK CUMIN (Nigella sativa) AGAINST

XANTHINE OXIDASE INHIBITION ONHYPERURICEMIC MICE

11 B010 RATNA YULIANI UMS ANTIBACTERIAL ACTIVITY OF

COMBINATION OF CHLORAMPHENICOL AND

ETHANOLIC EXTRACT OF PACAR AIR

(Impatiens balsamina) LEAVES AGAINST

Escherichia coliAND Shigella sonnei

12 B011 TANTI AZIZAH UMS ANTIHYPERCHOLESTEROLEMIC EFFECT OF

MURBEI (Morus albaL.) LEAVES AND ITS

COMBINATION WITH SIMVASTATIN IN RATS

INDUCED BY PROPYLTIOURACIL AND HIGH

FAT DIET


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Current Breakthrough in Pharmacy Materials and Analysesxvii | P a g e


13 C001 HENING

PRATIWI

UNSOED OVERVIEW ABOUT ANTIBIOTICS

PRESCRIBING IN URINARY TRACT

INFECTION ROEMANI SEMARANG

HOSPITALS INPATIENTS

14 C003 RASMAYA

NIRURI

UDAYANA

UNIVERSITY

CLINICAL IMPROVEMENT AFTER

CEPHALOSPORINE THERAPY ON CHILDREN

WITH TYPHOID FEVER

15 D001 INDRI KUSUMA

DEWI

POLTEKKES

KEMENKES

SURAKARTA

ANTIOXIDANT ACTIVITY ETHYL ACETATE

EXTRACT OF SEED AND POD PARKIA (Parkia

speciosaHassk.) WITH DPPH (1,1 -

DIPHENYL - 2 - PICRYLHYDRAZIL) METHOD

16 D002 MUHAMMAD

ASWAD

TOYAMA

UNIVERSITY

A CLICK-TYPE COUPLING REACTION

BETWEEN THIOAMIDES AND SULFONYL

AZIDES AS A VERSATILE APPROACH TO

GENERATE NEW PHARMACOLOGICALLY

ACTIVE COMPUNDS

17 E001 HARYOTO UMS CYTOTOXIC ACTIVITY OF POLAR,SEMIPOLAR, AND NON POLAR FRACTION

ETHANOL EXTRACT OF LEAVES PLANTS

SALA (Cynometra ramifloraLinn.) AGAINTS

WiDr CELL

18 E002 RETNO

ARIANINGRUM

UNY CHALCONES DERIVATIVE WITH BROMO

SUBSTITUENT INDUCES APOPTOSIS IN

HeLaA CELLS

19 E003 TRESNA

LESTARI

STIKES BAKTI

TUNAS

HUSADA

SUB-CLONING OF ads GENE INTO

pETDUET1_cyp FOR CO-EXPRESSION IN

ESCHERICHIA COLI


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xviii | P a g e ICB Pharma II 31 October 2015


LIST OF POSTER PRESENTERS


1 A002 ANDI SRI SURIATI

AMAL

UNIV.

DARUSSALAM GONTOR

PREPARATION OF ARTIFICIAL SALIVA

FORMULATION

2 A004 ISKANDAR

ZULKARNAIN

UNIV.

MUSLIM

INDONESIA

EFFECT COGRINDING ON THE PHYSICAL

CHARACTERISTIC OF BINARY MIXTURE OF

PIOGLITAZONE HCl AND METFORMIN HCL

3 A007 SHOLICHAH

ROHMANI

UNIV.

SEBELAS

MARET

THE INFLUENCE OF EGGPLANT PEEL

EXTRACT (Solanum melongenaL.) AS

ANTIOXIDANT ON THE LOTION MATERIAL

4 B002 DONI WIBOWO UMS ANTIOXIDANT ACTIVITIES OF

NANOEMULSION CONTAINING EXTRACT

OF SAMBILOTO (Andrographis paniculata

(Burm F.) Ness.) AND MENIRAN

(Phyllanthus niruriL.) IN ALLOXAN-

INDUCED DIABETIC RATS5 B003 FEBBY LOVITA

SARI

UMS ACUTE TOXICITY STUDY OF

NANOEMULSION CONTAINING

SAMBILOTO (Andrographis paniculata)

LEAVES AND MENIRAN (Phyllanthus niruri)

HERBS EXTRACT IN WISTAR RATS

6 B005 INTAN CAHYANI STIFAR

YAYASAN

PHARMASI

THE EFFECT OF GIVING GLUCOMANNAN

PORANG TUBER (Amorphophallus

oncophyllusPrain ex Hook. F.) ON SGPT

AND SGOT LEVELS OF WISTAR MALE RATS

BLOOD INDUCED BY PARACETAMOL

7 B006 KEN LATIFA UMS THE PROFILE OF MDA

(MALONDIALDEHYDE) LEVEL IN RATS

GIVEN EXTRACT OF THYMI HERBS

(Thymus vulgaris[L.])

8 B012 WESTI FAJRIN

BAYU

NUGRAHAINI

UMS EFFECTIVENESS TEST of 70% ETHANOL

EXTRACT ANALGETIK RED DRAGON FRUIT

PEEL (Hylocereus polyrhizus Cortex) WITH

STRETCHING METHOD ON MALE MICE

SWISS WEBSTER STRAIN

9 B013 SAPTO YULIANI UAD EFFECTS OF TURMERIC ETHANOLIC

EXTRACT ON TRIMETHYLTIN-INDUCED

OXIDATIVE STRESS ON SPRAGUEY

DAWLEY RATS

10 B014 FAJAR KHOLIKUL

AMRI

UMS ANTIDIABETIC ACTIVITY OF

NANOEMULSION CONTAINING EXTRACT

OF SAMBILOTO (Andrographis paniculata

(BURM F.) NESS.) AND MENIRAN

(Phyllanthus niruriL.) IN ALLOXAN-

INDUCED DIABETIC RATS

11 C002 IDA AYU PEBRINA UMS RATIONALITY TREATMENT OF

ANTIBIOTICS FOR TREATMENT OF

DIARRHEA IN ADULT PATIENTS IN THE

INSTALLATION INPATIENT HOSPITAL X

SURAKARTA 2014


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Current Breakthrough in Pharmacy Materials and Analysesxix | P a g e


12 C004 RASMAYA NIRURI UDAYANA

UNIVERSITY

TRANSAMINITIS ASSOCIATED WITH HIGH

DOSE METHOTREXATE AND 6

MERCAPTOPURIN IN CHILDREN WITH

ACUTE LYMPHOBLASTIC LEUKEMIA

13 C005 SAKTYA AYU

PRACILLIA

UMS COST ANALYSIS AND DESCRIPTION

TREATMENT OF DIARRHEA IN PATIENTS

OF INPATIENT HOSPITAL dr. MOEWARDI

SURAKARTA BY BPJS PROGRAM IN 2014

14 D003 NANIK

SULISTYANI

UMS ANTIBACTERIAL COMPOUND PRODUCED

BY A SOIL BACTERIA ISOLATED FROM

RIZHOSPHERE OFZingiber officinale
http://localhost/ACEGRO~1/AppData/Local/Temp/Rar$DI01.603/daftar%20oral%20n%20poster%20presenter.xlsx#RANGE!_Toc432925913http://localhost/ACEGRO~1/AppData/Local/Temp/Rar$DI01.603/daftar%20oral%20n%20poster%20presenter.xlsx#RANGE!_Toc432925913http://localhost/ACEGRO~1/AppData/Local/Temp/Rar$DI01.603/daftar%20oral%20n%20poster%20presenter.xlsx#RANGE!_Toc432925913http://localhost/ACEGRO~1/AppData/Local/Temp/Rar$DI01.603/daftar%20oral%20n%20poster%20presenter.xlsx#RANGE!_Toc432925913http://localhost/ACEGRO~1/AppData/Local/Temp/Rar$DI01.603/daftar%20oral%20n%20poster%20presenter.xlsx#RANGE!_Toc432925913http://localhost/ACEGRO~1/AppData/Local/Temp/Rar$DI01.603/daftar%20oral%20n%20poster%20presenter.xlsx#RANGE!_Toc432925913http://localhost/ACEGRO~1/AppData/Local/Temp/Rar$DI01.603/daftar%20oral%20n%20poster%20presenter.xlsx#RANGE!_Toc432925913http://localhost/ACEGRO~1/AppData/Local/Temp/Rar$DI01.603/daftar%20oral%20n%20poster%20presenter.xlsx#RANGE!_Toc432925913http://localhost/ACEGRO~1/AppData/Local/Temp/Rar$DI01.603/daftar%20oral%20n%20poster%20presenter.xlsx#RANGE!_Toc432925913


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ICB Pharma II 31 October 2015Current Breakthrough in Pharmacy Materials and Analyses 1 | P a g e

SPEAKERS

NANOSTRUCTURED BIOMATERIALS FOR MEDICAL AND BIOLOGICAL

APPLICATIONS

Jackie Y. YingInstitute of Bioengineering and Nanotechnology

31 Biopolis Way, The Nanos, Singapore 138669

E-mail: [email protected]

Nanostructured materials have been developed for various medical and biological applications.They have been designed as stimuli-responsive drug delivery systems and sustained protein

delivery systems. Nanocomposite systems have also been derived to provide simultaneous drug

delivery and bioimaging functions as theranostic systems. Micellar nanocomplexes have been

synthesized with green tea-based ingredients as unique carrier materials that offer synergistictherapeutic effects with the drugs to be delivered.

In addition, nanostructure processing has been employed in creating synthetic cell culture

substrates for the expansion and controlled differentiation of stem cells. Nanostructuredscaffolds have also been obtained for cell and tissue engineering.


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2 | P a g e ICB Pharma II 31 October 2015


SPEAKERS

PHOTOAFFINITY LABELING-BASED TARGET IDENTIFICATION OF BIOACTIVE

MOLECULES

Takenori TomohiroGraduate School of Medicine and Pharmaceutical Sciences

University of Toyama

Email: [email protected]

Target identification and confirmation for small molecules is often the rate-limiting step in drug

discovery. Photoaffinity labeling (PAL) is a photochemical method to attach a tag with a

covalent bond into the ligand-interacting surface within target protein. Since PAL can access to

most of biological interacting systems including membrane protein or weak-binding proteinthat are difficult to access by conventional affinity purification methods, it may be a powerful

method for identification of target protein that is also able to determine its interacting site.However, conventional PAL-based identification method accompanied with isolation of the

target in very pure form often failed, especially in case of the target of a trace amount. Thepurification process is often complicated by inevitable contamination that is due to non-specificadsorption and significant loss during handling because of the different physical properties of

individual peptide fragments.

Recently, we designed a unique photoreactive unit that can install a high-performance chemicaltag, an isotope-coded fluorescent tag, to the interacting surface upon UV-irradiation. The

combination of PAL and heterogeneous target-selecting techniques significantly simplified the

procedure and reduced the amount of quantity and time required for identification. The details

will be presented.


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SPEAKERS

CURRENT STATUS OF RECOMBINANT THERAPEUTIC PROTEINS AND LOCAL

ISSUES RELATED

Wangsa Tirta IsmayaRecombinant Therapeutic Proteins, Dexa Laboratories of Molecular Sciences, Dexa Medica

Industri Selatan V PP-7, Jababeka II Industrial Estate

Cikarang 17550, Indonesia

E-mail: [email protected]

Generation of recombinant version of a therapeutic protein (rTP) has been long employed tosolve issues with availability of the protein from its natural resource. Such approach results in

production of the protein in large scale of controlled, engineered, and directed manner.However, cost of both generation and production of the biological drug is high, thereby protein

theraphy is still not accessible for patients of limited income. Nevertheless, numerous rTPs have

been produced in the past decades and their demands keep on increasing.

Expiration of patents protecting current rTP allows production of its generic version called

biosimilar, which can be made available at lower prices from reduced costs of productdevelopment and of pre-clinical and clinical trials. Production of biosimilar is done through

processes obtained from technology transfer or self-developed. Either way, validation of the

biosimilar product remains the major challenge. Biosimilar product must share identicalcharacteristics and molecular properties, and display similar safety and potency features to

those of originator. Alterations in the physico-chemical properties of the molecule orimprovement in potency of the drugs are not permitted.

On the other hands, development of new recombinant therapeutic protein continues as new

diseases or new variant of a disease emerge as well as reoccurrence of well-known diseases.

Developing a recombinant therapeutic protein is usually started with search of candidates

based on putative or known molecular basis of a disease. We are currently developing a newapproach by means of employing proteins of unknown function to screen for their potential use

in pharmaceutic or therapy. This approach involves various techniques in bioinformatics,biochemistry, molecular biology and pharmacology, and cell physiology. This approach may

lead to discovery of novel pharmaceutical or therapeutic proteins. With the finding of manygenes that encode proteins of unknown function during elucidation of genomes (e.g. human),this unusual approach provides alternative to discover new pharmaceutical/therapeutic

proteins. We may as well assign possible biological function of the proteins.

In Indonesia, development of biosimilar or recombinant therapeutic protein is still at infancy.However, pharmaceutical industries embrace development and production of biosimilar,

especially to anticipate the coming Asean Economic Community implementation. The National

Agency for Food and Drugs Control (NAFDC) has been developing and preparing guidelines to

regulate and control the development and production of both biosimilar and rTP. Related toIndonesian moslem population in particular, development and production of halal certified

drugs, including rTP and biosimilar are also part of the major issues. Especially after recently

the bill for halal consumer products has been passed. Thus, development of recombinant

therapeutic protein appears to gain its momentum in Indonesia in the near future.


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OP A001 PHARMACEUTICAL TECHNOLOGY

CHARACTERISTICS TESTING OF MICROCRYSTALLINE CELLULOSE FROM NATA DECOCO COMPARED TO AVICEL pH 101 AND AVICEL pH 102

Adi Yugatama1*, Laksmi Maharani2, Hening Pratiwi2, Lingga Ikaditya31Farmasi, FMIPA, Universitas Sebelas Maret, Surakarta

2Farmasi, FIKES, Universitas Jenderal Soedirman, Purwokerto3Farmasi, Poltekkes Kemenkes Tasikmalaya, Tasikmalaya

*E-mail: [email protected]

Abstract

Microcrystalline cellulose is an imported raw material in Indonesia, which used widelyas an excipient in tablet production. One of the alternative materials to produce microcrystalline

cellulose is from nata de coco. This research aimed to know the characteristic ofmicrocrystalline cellulose from nata de coco compared to avicel pH 101 and avicel pH 102. Nata

de coco were alkalinated, dried and hydrolyzed to get microcrystalline cellulose. Independentvariables in this research are microcrystalline cellulose from nata de coco, avicel pH 101 and

avicel pH 102. While the dependent variables are flow properties, compactibility,compressibility, water absorption, tap density, bulk density, loss of drying, infrared absorption

spectra, and SEM images. Data was analyzed using one way ANAVA with CI 95% and using

software SPSS for windows. The result showed that the characteristic of microcrystallinecellulose from nata de coco is different in flow properties, compactibility, compressibility, tap

density, bulk density, and loss of drying from avicel pH 101 and avicel pH 102, but having thesame water absorption. Infrared spectrum data showed that microcrystalline cellulose from

nata de coco is similar to avicel pH 101 and avicel pH 102. The SEM result showed thatmicrocrystalline cellulose from nata de coco having bigger particle size (66.67266.67 m) than

avicel pH 101 (13.33166.67 m) and avicel pH 102(13.33200 m).

Keywords: Avicel pH 101, Avicel pH 102, Nata de coco, microcrystalline cellulose.


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PP A002 PHARMACEUTICAL TECHNOLOGY

PREPARATION OF ARTIFICIAL SALIVA FORMULATION

Andi Sri Suriati Amal1*, Samsinah Hj. Hussain2, Mohd. Amin Jalaluddin31Universitas Darussalam Gontor, Indonesia

2Universiti Malaya, Malaysia3Universiti Malaya, Malaysia


Abstract

Dry mouth or throat (xerostomia) is a clinical condition characterized by desiccation of

the intraoral tissues. Patients with chronic or temporary sensation of dry mouth need somekind of treatment to relieve the symptoms. Causes of dry mouth include medications,

autoimmune disease (Sjogrens syndrome), radiotherapy or chemotherapy for cancer, hormonedisorders and infections. The project is important not only because saliva substitutes are not

manufactured locally, but also because most saliva substitutes use mucin (porcin in origin).Therefore there is a need to produce one with other source which has properties to mucin itself.

The objective of this project is to produce saliva substitutes that can serve as mouth and throatlubricants. The first step was pre-formulation studies that involved characterization of active

ingredients (physical, chemical, and mechanical properties) in order to choose what otheringredients (excipients) should be used in the preparation. Formulation studies also considered

such factors as solubility, viscosity, and pH. The last step was assessment of safety and stabilityof the final product.The new artificial saliva formulations containing various ratios of SCMC(Sodium carboxymethyl cellulose), MC (methyl cellulose) and HPMC (hydroxypropyl

methycellulose) have been developed. Combination of cellulose derivatives and albumin in

these formulations resulted in the physical properties of these new artificial saliva substitutes

closely resembling human saliva and mucin-based saliva substitutes. Formula we choose werethe most suitable formulae due to their viscosity and pH properties which closely resemble

human saliva and mucin based saliva substitutes.

Keywords: artificial saliva, saliva substitute, mouth and throat lubricant, mouth and throatmoisturizer.


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FORMULATION OF ETHANOL EXTRACT OF TUNJUK LANGIT (Helminthostachys

zaylanica) RHIZOME AS TABLET DOSAGE FORM BY WET GRANULATION METHOD

Fitrya1*, Najma Annuria Fithri1, Budi Untari1, Aprililianti11Department of Pharmacy, University of Sriwijaya

Indralaya, OI, South Sumatera, Indonesia


Abstract

Tunjuk langit rhizome (Helminthostahcys zeylanica (Linn) Hook) has been used

traditionally as an anticancer and antiinflamatory agent. In addition, previous studies haveproven the potency of ethanol extract from the tunjuk langit rhizome (Helminthostahcys

zeylanica (Linn) Hook)as antihyperuricemic agent. In this study, ethanol extract of the rhizome

was formulated into a tablet dosage form by a wet granulation method. The tablet was prepared

with different types of disintegrant and binder, i.e. Formula A (starch: PVA), Formula B

(AvicelPH102: PVP), and Formula C (sodium alginate: methylcellulose). Physical properties(such as weight variation, tablet diameter, thickness, friability, hardness, and disintegration

time) and dissolution of tablets were evaluated. The results showed that tablet A (starch: PVA)

produced the best physical properties and dissolution characteristics, which have met the

requirements. Therefore, the wet granulation method is suitable to develop the extract intotablet.

Keywords: extract, tunjuk langit rhizome, Helminthostachys zaylanica, tablet, wet granulation.


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EFFECT COGRINDING ON THE PHYSICAL CHARACTERISTIC OF BINARY MIXTUREOF PIOGLITAZONE HCl AND METFORMIN HCl

Iskandar Zulkarnain1*, S. N. Soewandhi1, S. Wikarsa21Fakultas Farmasi Universitas Muslim Indonesia

2Sekolah Farmasi Institut Teknologi Bandung


Abstract

This study was aimed to identify the physical interaction between PGZ-MFN by

cogrinding. Solid state interaction was observed by cold contact method and phase diagramformation. The solid state grinding (SSG) and solvent drop grinding (SDG) was conducted on

binary mixtures. The identification resulted in a binary system was characterized by differential

thermal analysis (DTA), polarization microscopy, scanning electron microscope (SEM) andpowder X-ray diffraction (PXRD). Furthermore, the solubility and dissolution testing of PGZ

performed on the physical interaction results. The observation under the polarizing microscope

showed that the new crystal habit was not found. The mixture has melting point lower than

MFN and PGZ. This phenomenon was then confirmed with phase diagram arranged bythermogram of PGZ-MFN. That was identified mixture binary equimolar as eutectic mixture

with eutectic temperature 187 C. Meanwhile, PXRD data at equimolar mixture did not showed

the new interference peak. The DTA and powder X-ray diffraction data of the equimolar solid

compounds obtained from several tehnique showed similar result. The thermogram of alltreated sampels had similar endothermic curve (185- 186C), and identical interference peaks

of PGZ-MFN of 2 8.6; 12.2; 12.8; 17.2; 18.6; 22.7; 23.2 . Conclusion Based on the results of

morphological analysis, the PXRD data and thermal properties, PGZ-MFN equimolar mixtureshowed the formation of a eutectic mixture.

Keywords: Cogrinding, physical characteristic, binary system, pioglitazone HCl, metformin HCl.
mailto:[email protected]:[email protected]


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PREPARATION AND CHARACTERIZATION OF SUBMICRON PARTICLES OF PLGA

INCORPORATING RIFAMPIN USING EMULSION SOLVENT DIFFUSION METHOD

Mardiyanto1*1Department of Pharmacy, Faculty of Mathematics and Natural Science,University of Sriwijaya

Inderalaya, Indonesia


Abstract

The research had been performed to incorporate rifampin into PLGA submicron-sized

particles. This research has a prospect to be applied to overcome the ineffectiveness use ofrifampin for tuberculosis patients as rifampin was not stable in human lung macrophages, while

Mycobacterium tuberculosis was able to survive in human lung macrophages. Rifampin wasincorporated into submicron particles of PLGAs using the emulsion solvent diffusion method.

The use of rifampin 50 mg in every batch resulted in the submicron-sized particles of 220 nm,PDI 0:12, zeta potential 21 mV and EE 37%. In the batch using rifampin 300 mg, resulted the

submicron-sized particles of 410 nm, PDI 0:22, zeta potential 14 mV and EE 40%. The surface ofthe particles was visualized by SEM and hydrodynamic size compared to TEM. It was known

that particle is spherical with a smaller diameter than the hydrodynamic size. TEMmeasurement revealed the size of particles with PVA was 208 nm.

Keywords:characterization, PLGA, rifampin, hydrodynamic-size, TEM, %EE.


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LIQUID BATH SOAP FORMULATION AND ANTIBACTERIAL ACTIVITY TEST

AGAINSTStaphylococcus aureusOF KECOMBRANG(Etlingera elatior(Jack) R.M.Sm.) FLOS EXTRACTS

Lilis Handrayani1, Ratih Aryani1*, Indra11Pharmacy Study Programme, Sekolah Tinggi Ilmu Kesehatan Bakti Tunas Husada Tasikmalaya

*E-mail :[email protected]

Abstract

The formulation of kecombrang flos extract (Etlingera elatior (Jack) R.M.Sm.) liquid

bath soap has been established. The objective of this research was to formulate liquid bath soapof kecombrang flos extract (Etlingera elatior(Jack) R.M.Sm.) and to test its antibacterial activity

to Staphylococcus aureus. Kecombrang flos extract was extracted by maceration method using

96% ethanol, and followed by minimum inhibitory concentration (MIC) test using hole method.

Concentration variation of kecombrang flos extract was conducted as F1 (6%), F2 (8%), and F3(10%). The formula of liquid bath soap of kecombrang flos extract was evaluated using several

examinations such as organoleptic, pH, viscosity, density, foaming stability, antibacterial activity

test, irritation test and hedonic test. The result shows the liquid bath soap of kecombrang flosextract F1, F2 and F3 can inhibit the growth of Staphylococcus aureus. Based on statistical test

using SPSS 21 (for trial) ANOVA method continued by LSD shows that F0 (negative control), F1,

F2, F3 and positive control (triclosan 2.5%) have difference meaningful result with significance

value < 0.05.

Keywords: Kecombrang flos extract, Etlingera elatior, liquid bath soap, antibacterial,

Staphylococcus aureus.


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THE INFLUENCE OF EGGPLANT PEEL EXTRACT (Solanum melongenaL.) ASANTIOXIDANT IN LOTION DOSAGE FORM

Sholichah Rohmani1*1Universitas Sebelas Maret

Solo, Indonesia


Abstract

The purpose of this research is to formulate lotion containing extract of eggplant peelas an effective and safe antioxidant lotion. Previous research showed that the anthocyanin in

peel eggplant has antioxidant activity against free radical that cause aging on the skin. Extract of

eggplant peel was obtained by maceration process using ethanol 70%. The extract was added invarious concentrations in lotion formulation i.e. 0%, 0.5%, 1%, 2% and 3% based on the

antioxidant activity that was determined using DPPH method. The produced lotions werecharacterized for the antioxidant activity, stability and physical properties including

organoleptic, viscosity, pH and panellist acceptability. The result showed that antioxidantactivity rated weekly and decreasing viscosity from week 0 to 8, pH of 5.62-6.93 was observed.

Formulae I was the most acceptable lotion by pannelist.

Keywords: peel eggplant, lotion, antioxidant.


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OPTIMIZING COMBINATION OFSAMBILOTOHERBAL WATER FRACTION ANDSALAMLEAF WATER FRACTION AS ANTI-INFLAMMATION

Raymond Harris Mustafa1*, Lannie Hadisoewignyo1, Martha Ervina1, Lisa

Soegianto1, Wahyu Dewi Tamayanti11Faculty of Pharmacy, Widya Mandala Surabaya Catholic University

Surabaya, Indonesia


Abstract

Sambiloto herbs (Andrographis paniculata Nees) and salam leaves (Syzygiumpolyanthum), which are effective to reduce blood sugar level with different mechanism, have

been suggested to produce a synergy as antioxidant and anti-inflammatory agent, hence theoptimum combination formula is remained to be elaborated. By reducing blood sugar level and

showing antioxidant, and anti-inflammatory activity, both plants were hypothesized of its

ability for ameliorating diabetes mellitus complexicity. This study aimed to discover the optimal

combination formula of sambiloto herbs (Andrographis paniculata Nees) and salam leaves(Syzygium polyanthum) water fraction to produce anti-inflammation effect. Carrageenan was

used to induced the inflammatory condition. Optimization was conducted by factorial design

utilising 2 factors and 2 levels. Both factors, sambiloto herbs (Andrographis paniculata Nees)

and salam leaves (Syzygium polyanthum) in the range of low level 1:10 and high level 10:1 with

the used doses were 100 mg/kg BW at low level and 300 mg/kg BW for high level. The observedparameters were anti inflammation potential percent and edema rate. Conclusively, this study

proposed that optimum combination formula of sambiloto herbal : salam leaves water fraction =1.14 : 9.87 with 234 mg dose. The combination formula was theoretically resulted the optimum

anti inflammation potential percent of 45.68%, and ER of 38.39% compared with othercombination formulas.

Keywords: salamleaf, sambilotoherbal, water fraction, anti inflammation, factorial design.


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OP B001 PHARMACOLOGY AND MICROBIOLOGY

THE POTENTIAL OF THE BLACK RICE BRAN EXTRACT AS ANTIDIABETIC AGENT

Arifah Sri Wahyuni1*, Rima Munawwaroh1, Esthi Utaminingsih1, Novita Sari11Faculty of Pharmacy University Muhammadiyah Surakarta


*Email: [email protected]

Abstract

Natural hypoglycaemic compounds may be attractive alternatives to synthetic drugs orreinforcements to currently used treatments. Black rice bran is one of natural compound that

containing anthocianins. The major anthocyanins component isolated from black rice bran iscyanidin 3-glucoside. In this experiment, fiveteen male rat were randomly allocated into 5 equalgroups. They were induced by alloxan (150mg/kgbw, i.p) except Group I). If the blood glucose

levels reach 200 mg/dL, they were given aquadest (Group II), black rice bran extract (BBE)

respectively 50, 100 and 200 mg/kg.bw (Group III-V). Blood glucose level was analyzed at 4, 7,

10 days treatment. Insulin level was determined enzyme linked immunosorbent assay (ELISA).The result showed that baseline levels of blood glucose were statistically not different among

the fivegroups (p>0.05). By the end of experiment BBE dose 50 mg/kgbw did not reduce bloodglucose levels after 10 days of treatment (p>0.05). BBE dose of 100 and 200 mg/kgbw

decreased blood glucose levels. The level of blood glucose after administered 10 days by BBE200 mg/kgbw was 131.33 8.08 mg/dL.The results showed that insulin levels in diabetic rats

increased by administration of the extract at dose 200 mg/kg b.w was 15,209,5ng/mL.It can

be concluded that BBE can be therapeutically helpful as antidiabetic agents

Keywords: cyanidin 3-glucoside, black rice bran extract, antidiabetic.


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PP B002 PHARMACOLOGY AND MICROBIOLOGY

ANTIOXIDANT ACTIVITIES OF NANOEMULSION CONTAINING EXTRACT OFSAMBILOTO (Andrographis paniculata (Burm F.) Ness.) AND MENIRAN

(Phyllanthus niruriL.)IN ALLOXAN- INDUCED DIABETIC RATS

Erindyah R Wikantyasning1*, Nurcahyanti Wahyuningtyas1, Muhammad Dai1,

Doni Wibowo11Faculty of Pharmacy, Universitas Muhammadiyah Surakarta


*E-mail : [email protected]

Abstract

Combination of an oral antidiabetic with antioxidant is potential to treat disease andthe complications of diabetes mellitus. The aim of this study was to investigate the antioxidant

activities of nanoemulsion containing extract of sambiloto (Andrographis paniculata (Burm F.)Ness.) and meniran (Phyllanthus niruri, L.) (NESM) in alloxan-induced diabetic rats. Rats were

divided into 6 groups that were orally administered by nanoemulsion base (NE), vitamin E (100mg/kg), NESM (100, 200 and 400 mg/kg) and combination of extract of sambiloto and meniran

(ESM) (400 mg/kg) for 14 days. Blood was withdrawn on day 0, 1, and 14, and malondialdehyde(MDA) level was determined using UV-Vis spectrophotometer. The result showed thatincreasing dose of NESM reduced plasma MDA levels compared to NE, vitamin E and ESM

groups. It can be concluded that NESM possess antioxidant activities.

Keywords: nanoemulsion, Andrographis paniculata, Phyllanthus niruri, malondialdehyde(MDA), antioxidant.


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ACUTE TOXICITY STUDY OF NANOEMULSION CONTAINING SAMBILOTO (Andrographis

paniculata) LEAVES AND MENIRAN (Phyllanthus niruri) HERBS EXTRACTIN WISTAR RATS

Erindyah R. Wikantyasning1*, Nurcahyanti Wahyuningtyas1, Muhammad Dai1,

Febby Lovita Sari11Faculty of Pharmacy, Universitas Muhammadiyah Surakarta


*Email: [email protected]

Abstract

Andrographis paniculata leaves (AL) and Phyllanthus niruri herbs (PH) have been

proven scientifically to have antidiabetic and antioxidant activities. Nanoemulsion system has

been developed containing of both AL and PH extracts, using Tween 80 and polyethylene glycolas surfactant and cosurfactant, respectively. The present study is to investigate the acutetoxicity of the nanoemulsion on Wistar rats. Five groups of rats were orally treated with

nanoemulsion doses of 31.25, 125, 500, 2000 mg/kg and control. General behavior, adverse

effects and mortality were observed for up to 14 days. Oral administration of nanoemulsion at

the highest dose of 2000 mg/kg resulted in no mortalities or evidence of adverse effects,indicated that nanoemulsion is nontoxic with a LD50 higher than 2000mg/kg. Throughout 14

days of the treatment, there was no significant change in behavior and body weight of rats inboth treatment and control groups. Histopalogical study showed that there were congestion in

the liver and inflammation in the kidney by the highest dose of 2000 mg/kg. In addition, thepancreas and gastric of rats in the highest dose group showed a significant change compared to

the control group.

Keywords: acute toxicity, nanoemulsion, A. paniculata, P. niruri, herbal extract.


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ANTI-INFLAMMATORY ACTIVITY TEST OF CHRISTMAS PALM (Adonidia merrillii(Becc.) Becc.) SEED EXTRACT IN MALE WISTAR RATS (Rattus norvegicus)

Herlina1*, Fitrya1, Fithri Najma A1, Rahmawati Dwi Shafarina11Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Sriwijaya University

South Sumatera, Indonesia

*Email :[email protected]

Abstract

Christmas palm (Adonidia merrillii (Becc.) Becc.) contains flavonoids, tannins,

triterpenoids, and steroids. Antioxidant activity of christmas palm has been investigated. Basedon research, antioxidant has a potential as an anti-inflammatory agent. Christmas palm seed

extract anti-inflammatory activity test with rat paw edema method which induced by

carragenaan has been done. Ethanolic, ethyl acetate, and n-hexane extract of christmas palmwere divided into three dose groups, 200 mg/kg BW, 400 mg/kg BW, and 800 mg/kg BW.

Negative control was CMC Na in distilled water and positive control was 0.82 mg/200 g BW

diclofenac Na. Thirty three Wistar male rats as test subjects were divided into 11 groups (1

negative control group, 1 positive control group, and 9 treatment groups). Anti-inflammatoryactivity was tested by measuring the volume of rat paw edema using plethysmometer. The test

results showed the average of inhibition percentage of the ethanolic, ethyl acetate, and n-hexane

extracts were 46.05%; 29.95%; and 34.63% respectively. This shows that the ethanolic extract

has better inflammation inhibitory activity than ethyl acetate and n-hexane extracts. Non-parametric statistical Mann Whitney test of 800 mg/kg BW ethanolic extract at 30 and 60showed that both were not significantly different (p>0.05) to diclofenac Na. Christmas palm

ethyl acetate extract has anti-inflammatory activity far below diclofenac Na, but its effect wasconstant until 150. n-hexane extract of 800 mg/kg BW at 60 showed anti-inflammatory effect

that was almost equivalent to diclofenac Na (p>0.05). Christmas palm seed extract ED50 hasbeen calculated based on the average of edema inhibition percentage in rats. ED 50of christmas

palm ethanolic extract is 640.42 mg/kg BW.

Keywords:Adonidia merrillii (Becc.) Becc., anti-inflammatory, rat paw edema, diclofenac Na.


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THE EFFECT OF GIVING GLUCOMANNAN PORANG TUBER (Amorphophallusoncophyllus Prain ex Hook. F.) ON SGPT AND SGOT LEVELS OF MALE WISTAR RATS

BLOOD INDUCED BY PARACETAMOL

Intan Martha Cahyani1*, Bekti Nugraheni11Sekolah Tinggi Ilmu Farmasi YAYASAN PHARMASI

Semarang, Indonesia


Abstract

Indonesia is a country with a high prevalence of liver disease. Liver is important for

survival and plays a role in every metabolic function of the body. One of the causes of liver

disorder is because of drugs. One of the drugs that cause liver damage is paracetamol.Glucomannan is a major component of the porang tuber that serves as a soluble fiber.

Glucomannan is thought to have hepatotherapy effects as a potential antioxidant. The aim ofthis study is to determine the effect of glucomannan porang tuber (Amorphophallus oncophyllus

Prain ex Hook. F.) with graded doses on the blood levels of SGPT and SGOT wistar male ratsinduced by paracetamol dose of 1,638 g/kg BB. This research was experimental study. The

treatment in this study was porang glucomannan with a dose of 25 mg / kg, 50 mg / kg and 100

mg / kg. The Population of the study was white male Wistar rats aged 3-4 months with a weight

of 180g - 250g, and healthy. The result of the analysis showed that glucomannan Porang

(Amorphophallus oncophyllus Prain ex Hook. F.) has an effect as hepatotherapy. Givingglucomannan porang at a dose of 50 mg / kg rat has an effect to decrease blood levels of SGPTand SGOT wistar male rats induced by paracetamol.

Keywords: Porang tuber (Amorphophallus oncophyllusPrain ex Hook. F.), SGPT, SGOT.


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THE PROFILE OF MDA (malondialdehyde) LEVEL IN RATS GIVEN EXTRACT OFTHYMI HERBS (Thymus vulgaris[L.])

Ken Inayati Latifa1*, Tanti Azizah Sujono1, Ika T. Dian Kusumowati11Faculty of Pharmacy, Universitas Muhammadiyah Surakarta



Abstract

Oxidative stress occurs because of an imbalance between oxidants and antioxidants

which are then potentially cause damage to cells and reportedly play an important role in theprocess of liver damage. Free radicals can increase lipid peroxidation, which will then be

decomposed into malondialdehyde (MDA) in the blood. MDA is a marker of cell damage causedby free radicals. Extract of Thymi contains chemical compounds such as terpenoids, flavonoids,

aglycone, and phenolic acids. The content of flavonoid compounds contained in herbal Thymican serve as an antidote to the radical. The purpose of this study is to determine the effect of

herbal extracts Thymi in rats MDA levels. The study used 20 rats were divided into 4 groups.Group I was a normal control group was given distilled water of 2.5 ml/kg, while group II-IV

were given Thymi herbal extract for 5 days with a dose of 50, 100, and 150 mg/KgBW,

respectively. Blood was drawn for MDA content measurement using spectrophotometer at awavelength of 530 nm. The results showed that herbal extracts Thymi can reduce levels of MDA

in groups II, III, and IV. Meanwhile, there was also a decrease of MDA level in grup 1. So in thisstudy decreased levels of MDA which occurs in group II, III, and IV can not be said as a result of

administration of herbal extracts Thymi due to a decrease in MDA levels also occurred in groupI were only given distilled water.

Keywords: Thymus vulgaris, MDA (malondialdehyde).


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PRE-CLINICALSTUDY OF Cr (III) BASED HYPOGLICEMIC SUPPLEMENT IN-TYPE 2DIABETIC RATS

Kun Sri Budiasih1*, Kartika Ratna Pertiwi11Faculty of Mathematics and Natural Sciences, Yogyakarta State University

Yogyakarta, Indonesia


[email protected]

Abstract

The chromium (III)- amino acid complex based hypoglicemic agent was investigatedon nicotinamide-streptozotocin induced diabetic Wistar rats. The rats were divided into 7

groups each consist of 4 animals. Three groups are control (+) with chromium picolinate(CrPic), control (-) diabetic group (DM), and control non diabetic (non DM). Furthermore, three

groups were examined on the effect of Cr-AA[Cr(-OH)(glu)(OH)2]26H2O at dose of (50, 150and 300 g/day). In addition, the last group was studied on the effect of control group by

glibenclamide. The blood glucose levels were measured before and after treatment. The resultsshow that supplementation of Cr(III)-complex in 8 weeks decreased the blood glucose level in

the range of 46.446 79.593 %.

Keywords: hypoglicemic, chromium (III), amino acid, complexes, nicotinamide-streptozotocin,

diabetic rats.


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SCREENING ANTIBACTERIAL POTENCY OF ENDOPHYTIC FUNGI METABOLITE OF

MANGOSTEEN (GarciniamangostanaL.) LEAF

Lisa Soegianto1*, Martha Ervina1, Kevin Widjaja1, Angela Violita11Faculty of Pharmacy, Widya Mandala Catholic University Surabaya

Surabaya, Indonesia


Abstract

Mangosteen (Garcinia mangostanaL.) is a tropical plant which its fruit peel is widely

used as an antioxidant, anti-diarrhea, anti-inflammatory, antitumor, and as an antibacterial. The

previous study found the antibacterial activities of extract and metabolites of endophytic fungiof mangosteen rind. This research was aimed to explore utilization of mangosteen leaf and to

screen antibacterial potency of the mangosteen leaf endophytic fungi metabolites. Endophytic

fungi were isolated from leaf of mangosteen in the Malt Extract Agar (MEA) medium in order to

get 2 colonies of endophytic fungi. Screening of potential antibacterial metabolites was assessedusing diffusion method and bioautography, obtained results of the antibacterial activity against

Escherichia coli(Ec) and Staphylococcus aureus(Sa) of the metabolites endophytic fungi of leaf.

Macroscopic and microscopic characteristics from fungi isolate which has antibacterial potency,

was observed and fermented into Potatoes Dextrose Yeast (PDY) medium for 14 days. At day14, biomass and supernatant were separated and carried out separation by liquid-liquidextraction. The supernatant and biomass were fractionated using n-hexane, ethyl acetate, and

water. Each fraction was eluated to several mobile phase and tested its antibacterial activityagainst Ec and Sa. The result showed that there is a potential antibacterial activity of endophytic

fungi metabolites leaf ED2 against Sa. Bioautography result was observed that the compoundhas antibacterial activity and is supposed as flavonoid compounds. It was supposed that

endophytic fungi ED2 was a group of Trichoderma.

Keywords: mangosteen (Garcinia mangostana L.), endophytic fungi, antibacterial activity,Escherichia coli, Staphylococcus aureus.


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ACTIVITIES OF THE COMBINED EXTRACTS OF TEMPUYUNG (Sonchus arvensis)

AND BLACK CUMIN (NIGELLA SATIVA) AGAINST XANTHINE OXIDASE INHIBITIONON HYPERURICEMIC MICE

Muhtadi1*, Nurcahyanti Wahyuningtyas1, Andi Suhendi1, Septi Heryani11Faculty of Pharmacy, Universitas Muhammadiyah Surakarta



Abstract

The combination extracts of tempuyung (Sonchus arvensis) and black cumin (Nigellasativa) can decrease uric acid levels on the previous result research. However, the mechanism of

decreasing uric acid levels was unknown certainly. This study aimed to determine the inhibitory

effect of xanthine oxidase by the combination extracts of black cumin and tempuyung on

hyperuricemic mice. Hyperuricemic mice were induced by 250 mg/kgBW potassium oxonate

was given one hour before treatment. The mice were divided into 3 groups, group I was

given 10 mg/kgBW allopurinol (positive control), group II was given 0.5 mL/20gBW aquadest

(negative control) and group III was given the combination extracts of black cumin-tempuyung

with dose 200 mg/kgBW during 4 days administration. The supernatant of liver was taken and

measured of xanthine oxidase levels by spectrophotometer UV at 290 nm. The data of xanthine

oxidase activity were analyzed by Kruskal-Wallis and Mann-Whitney method. Xanthine oxidase

activity of the combination extracts of blackcumin-tempuyung was 4.540.9 U/mg, verysignificantly than control negative was 8.000.22 U/mg (p


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ANTIBACTERIAL ACTIVITY OF COMBINATION OF CHLORAMPHENICOL AND

ETHANOLIC EXTRACT OF PACAR AIR (Impatiens balsamina) LEAVES AGAINSTEscherichia coliANDShigella sonnei

Ratna Yuliani1*, Agung Cokro Prabowo1, Yeni Maisyah11Faculty of Pharmacy, Universitas Muhammadiyah Surakarta



Abstract

Chloramphenicol, a broad spectrum antibiotic, is used to treat several bacterialinfections. However, it has several side effects such as headache, rash, diarrhea, nausea,

vomiting, bone marrow suppression, and aplastic anemia. Combining antibiotic with another

antibacterial agent may decrease the dose thus the side effects. This study aimed to investigate

the antibacterial activity of chloramphenicol combined with ethanolic extract of pacar air(Impatiens balsamina) leaves against Escherichia coliand Shigella sonnei. Chloramphenicol (30

g), extract (2500 g), combination of chloramphenicol and extract (22.5 g + 625 g and 15 g+ 1250 g), dimethylsulfoxide, and water for injection were tested for antibacterial activity

against Escherichia coliand Shigella sonnei using well diffusion method. Data was analyzed usingMann-Whitney test.The results showed that only chloramphenicol (22.5 g) combined with

extract (625 g) has inhibition zone diameter, which was not significantly different from

chloramphenicol alone (30 g) when it tested against E. coli. This result indicated that

combination of the antibiotic in lower concentration and extract can achieve the sameantibacterial activity as antibiotic alone in higher concentration.

Keywords:antibacterial, chloramphenicol, Impatiens balsamina, combination.


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ANTIHYPERCHOLESTEROLEMIC EFFECT OF Murbei (Morus alba L.) LEAVES AND

ITS COMBINATION WITH SIMVASTATIN IN RATS INDUCED BY PROPYLTIOURACILAND HIGH FAT DIET

Tanti Azizah Sujono1*, Haryoto1, Ratna Kartikasari1, Laily Ieda Quntari11Faculty of Pharmacy, Universitas Muhammadiyah Surakarta


*E-mail:[email protected]

Abstract

Empirically Murbei leaves are used to treat diseases such as hypercholesterolemia.Murbei leaves contain quercetin 3- (6-malonylglucoside) and rutin which shows a strong

inhibitory effect on LDL oxidation. This study aimed to verify the effect of the ethanol extract ofmurbei leaves in lowering total cholesterol in hyperlipidemic rats and the effect of combination

extract murbei with simvastatin to the hypocholesterolemic effect of simvastatin. Twenty four

Wistar male rats were divided into 6 groups randomly, group I-VI were given high fat diet and

(propyltiouracil) PTU to induce hypercholesterolemia. Group I as positive control was treatedsimvastatin 3.6 mg/kgbw, group II as a negative control was given CMC Na, group III-V were

given ethanol extract of murbei leaves orally at a dose of 0.4; 0.6; and 0.8 g/kgbw respectively,group VI were treated a combination of murbei extract 0.4 g/kg with simvastatin. Total

cholesterol was measured using a spectrophotometer visible ( = 500 nm) with CHOD-PAP

method as many as 3 times, i.e on day 0 (baseline), 14 days after induced hypercholesterolemiawith PTU and high-fat-diet and 14 days after treatment of murbei extract with still be given PTU

and a high-fat diet. The results showed that murbei leaves extract dose 0.4; 0.6 and 0.8 g/kgwere able to reduce total cholesterol with percent decrease in cholesterol levels by 32.94

10.07; 40.17 4.61 and 45.71 4.27% respectively. Ethanol extract of murbei 0.4 g/kg canincrease hypocholesterolemic effect of simvastatin when used in combination (p< 0.05).

Keywords: Murbei (Morus albaL), antihypercholesterolemic, propyltiouracil, high fat diet.


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ANALGESIC EFFECT OF ETHANOLIC EXTRACT RED DRAGON FRUIT ( Hylocereus

polyrhizus Cortex) PEEL ON MALE MICE SWISS WEBSTER WITHWRITHING REFLEX METHOD

Westi Fajrin Bayu Nugrahaini1*, Tanti Azizah Sujono11Faculty of Pharmacy, Universitas Muhammadiyah Surakarta


*E-mail:[email protected]

Abstract

Red Dragon fruit (Hylocereus polyrhizus) is one of the plants that grow in Indonesia.Peel of fruit (Hylocereus polyrhizus) contain oligosacharida and Betasianin. Oligosacharida

showed activity as prebiotic, which can lower the resistance to acidic conditions in the stomach,meanwhile betasianin can protect the cells from damage caused by free radical. This research

aims to prove whether peel of Dragon fruit has activity as analgesic in mice. Twenty five micewere divided into 5 groups. group I was given Na CMC 1% (negative control), group II was

treated paracetamol 65 mg/kgBW (positive control), group III, IV, and V were given ethanolic

extracts of dragon fruit peel at dose 0.25, 0.5, and 1 g/kgBW respectively. Twenty minutes later,all mice were induced pain by 0,1 ml intraperitoneal acetic acid 1% and the cumulative of

writhing reflect of mice were calculated for one hour. Then percent inhibition of writhing were

analyzed by Kruskal Wallis test and continued by Mann-Whitney with 95% confidence level.

The results showed that ethanolic extracts of dragon fruit peel showed analgesic effect. It candecrease the number abdominal constrictions and also increased the percentage inhibition of

writhing at dose of 0.25, 0.5, and 1 g/kgBW with percent of inhibition 42,76 2,04; 1.42

49,32; and 61,38 1,37% respectively.

Keywords:Hylocereus polyrhizus, Dragon fruit peel, analgesic, writhing reflect


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EFFECTS OF TURMERIC ETHANOLIC EXTRACT ON TRIMETHYLTIN-INDUCED

OXIDATIVE STRESS ON SPRAGUEY DAWLEY RATS

Sapto Yuliani1*, Mustofa Mustofa2, Ginus Partadiredja21Faculty of Pharmacy, Universitas Ahmad Dahlan

2Faculty of Medicine, Universitas Gadjah Mada

Yogyakarta, Indonesia


Abstract

Oxidative stress, an imbalance between free radicals and the antioxidant system, isknown to contribute to the pathogenesis of the development of dementia. Ethanolic extract fromturmeric (Curcuma longaL.) containing the curcumin constituent has been reported to produce

antioxidant effects. This study aims to determine the effect of turmeric extract on markers of

oxidative stress in Spraguey Dawley rat induced by (trimethyltin) TMT. Thirty six adult male

Sprague-Dawley rats (195-215 g) were divided randomly into six groups consisting of 6 rats foreach group. The rats were divided randomly into six groups, i.e.: N group, which served as anormal control group; T group, which was given intra-peritoneal injection of TMT chloride; T-

Cit group, which was treated with oral citicoline and TMT chloride injection; and three T-TEgroups, which were treated with three different dosages of oral turmeric rhizome extract, as

well as TMT chloride.The turmeric rhizome extract and citicoline solutions were given at day 1up to day 28 of experiment, whereas the TMT chloride injection given as a single dose of 8 mg

/kg bw was administered at day 8 of experiment. At day 36 the blood were taken for plasmaMDA level determination. Afterthat all rats were sacrificed and the cerebral hemisphere were

then dissected out from the skull. The left cerebral hemispheres were used for biochemicalobservation i.e. MDA and GSH level, activities of SOD, catalase (CAT) and GPx. The turmeric

extract dose of 200 mg/kg bw could prevent oxidative stress induced by TMT through thedecrease of levels of plasma and brain MDA and increased the activities of SOD, CAT, GPx, and

the level of GSH of brain. These effects seem to be comparable to those of citicoline.

Keywords: Curcuma longaL., trimethyltin, oxidative stress.


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ANTIDIABETIC ACTIVITY OF NANOEMULSION CONTAINING EXTRACT OF

SAMBILOTO (Andrographis paniculata(BURM F.) NESS.) AND MENIRAN(Phyllanthus niruriL.) IN ALLOXAN-INDUCED DIABETIC RATS

Erindyah R. Wikantyasning1*, Nurcahyanti Wahyuningtyas1, Muhammad Dai1,

Fajar Kholikul Amri11Faculty of Pharmacy, Universitas Muhammadiyah Surakarta


*E-mail : [email protected]

Abstract

Preparation of nanoemulsion containing combination of extract of A. Paniculataand P.Niruri (NESM) has been successfully developed. The present study was carried out to investigate

the antidiabetic activity of the nanoemulsion preparation in alloxan-induced diabetic rats.Ratswere divided into 6 groups that were given oral administration of nanoemulsion base (NE),

glibenclamide (5 mg/kg), NESM (100, 200 and 400 mg/kg) and combination of extract ofsambiloto and meniran (ESM) (400 mg/kg) for 14 days. The hypoglycemic effect was measuredby blood glucose level. Oral administration of the NESM at a dose of 100, 200, and 400 mg/kg

daily for 14 days showed a significant (P


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OP C001 CLINICAL AND COMMUNITY PHARMACY

OVERVIEW ABOUT ANTIBIOTICS PRESCRIBING IN URINARY TRACT INFECTION

ROEMANI SEMARANG HOSPITALS INPATIENTS

Hening Pratiwi1*1Studied Program of Pharmacy UNSOED

Purwokerto, Indonesia


Abstract

Inappropriate antibiotics prescribing in Urinary Tract Infection (UTI) can lead antibiotics

resistance. Therefore, hospitals should have a formulary as a reference for providing medicalservices to the patients. This study aims to determine the types of antibiotics that prescribed for

UTI treatment on January until November 2009 and determine the level of antibiotics

prescribing conformity with the Roemani Semarang hospitals formulary and WHO 2001guidelines. This study used a non-analytical descriptive design and retrospectively. The sampleswere 73 patients. This study includes the pattern of antibiotic prescribing in UTI patients and

conformity with 2009 hospital formulary and 2001 WHO guidelines. The results showed that

antibiotics are widely used cefotaxime (cephalosporins) 14 cases (24%), levofloxacin(quinolones) 11 cases (18%), and ceftriaxone (cephalosporins) 10 cases (17%). The

combination that widely prescribed are cephalosporins combination with quinolones 3 cases

(21%), cephalosporin combination with other cephalosporins 3 cases (21%), combination of

cephalosporin with an aminoglycoside 2 cases (14%), there are 68 prescriptions (93%) suitablewith hospital formulary, and 5 prescriptions (7%) not listed on the formulary of Roemani

hospital 2009. The UTI antibiotic monotherapy in women, men, and children do not exist in

accordance with the WHO guidelines 2001.

Keywords: Urinary Tract Infections, antibiotics, hospital formulary, RS. Roemani Semarang.


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PP C002 CLINICAL AND COMMUNITY PHARMACY

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