3096.C - PicoBrite user guide - Horiba · 2011. 2. 10. · The Electrical Shock Symbol indicates a...

PicoBrite

Picosecond laser diodes and LEDs

User guide

USA: HORIBA Jobin Yvon Inc., 3880 Park Avenue, Edison, NJ 08820-3012, Toll-Free: +1-866-jobinyvon Tel: +1-732-494-8660, Fax: +1-732-549-5125, E-mail: [email protected], www.jobinyvon.com France: HORIBA Jobin Yvon S.A.S., 16-18, rue du Canal, 91165 Longjumeau Cedex, Tel: +33 (0) 1 64 54 13 00, Fax: +33 (0) 1 69 09 93 19, www.jobinyvon.fr Japan: HORIBA Ltd., JY Optical Sales Dept, Higashi-Kanda, Daiji Building, 1-7-8 Higashi-Kanda Chiyoda-ku, Tokyo 101-0031, Tel: +81 (0) 3 3861 8231, www.jyhoriba.jp Germany: +49 (0) 89 4623 17-0 Italy: +39 0 2 57603050 UK: +44 (0) 20 8204 8142 China: +86 (0) 10 6849 2216

User guide PicoBrite pulsed diode excitation sources

IBH.640.UG.3096.C Page 2 of 35 © HORIBA Jobin Yvon IBH / HORIBA Scientific

Table of contents

1. Safety precautions ............................................................................................................................ 3

1.1 Safety symbols ............................................................................................................................ 3

1.2 PicoBrite safety notice ................................................................................................................ 4 1.2.1 PicoBrite classifications and safety considerations .................................................................. 5

2. Introduction....................................................................................................................................... 8

3. Installation......................................................................................................................................... 9

3.1 Unpacking.................................................................................................................................... 9

3.2 PicoBrite head ........................................................................................................................... 10 3.2.1 Bayonet coupling ...................................................................................................................12 3.2.2 Pillar mounting .......................................................................................................................12 3.2.3 Optical fibre coupling .............................................................................................................13

3.3 PicoBrite controller.................................................................................................................... 14

3.4 USB driver and software installation ........................................................................................ 15 3.4.1 Driver installation....................................................................................................................16 3.4.2 Software installation (control panel emulator) .........................................................................17

4. Operating guide .............................................................................................................................. 19

4.1 Front panel controls and indicators.......................................................................................... 19 4.1.1 Key-operated switch and emission indicator...........................................................................19 4.1.2 LCD display and menu dial.....................................................................................................20 4.1.3 Status indicators.....................................................................................................................20

4.2 Rear panel connections............................................................................................................. 21

4.3 Parameter Menu ........................................................................................................................ 22

4.4 First time operation ................................................................................................................... 24

4.5 Software control panel .............................................................................................................. 26

5. Maintenance and troubleshooting.................................................................................................. 28

5.1 General....................................................................................................................................... 28

5.2 Troubleshooting ........................................................................................................................ 28 5.2.1 LCD Messages.......................................................................................................................28 5.2.2 Performance issues................................................................................................................29

6. Specifications.................................................................................................................................. 30

Appendix A: Optical filter ordering information................................................................................. 31

Appendix B: Fibre coupler adjustment............................................................................................... 33

Notes ................................................................................................................................................... 35



1. Safety precautions

1.1 Safety symbols

Terms and symbols described in this section are used throughout the HORIBA Scientific TCSPC product

line. These symbols may appear in this user guide only, or they may be marked on the outside of the

product or other equipment that is intended for use with the product.

The product will be marked with this Exclamation Symbol when it is necessary

to refer to this user guide in order to prevent personal injury or equipment

damage. This symbol also appears in this user guide to indicate areas where

personal injury or equipment damage is possible.

The Electrical Shock Symbol indicates a hazard arising from dangerous

voltage. Any mishandling could result in irreparable damage to the equipment,

and personal injury or death.

The Grounding Symbol indicates that connection to an external ground

conductor is required for protection against electric shock in case of a fault.

Failure to provide correct grounding or mishandling could result in irreparable

damage to the equipment, and personal injury or death.

The Laser symbol indicates the presence of radiation emitted from visible or

invisible laser light sources. This symbol may also be used to indicate the

presence of invisible ultraviolet or infrared light. Do not look directly at the

beam. Failure to follow laser safety rules or mishandling could result in eye

injury leading to permanent loss of sight or vision impairment.

WARNING This calls attention to a procedure, practice, or condition that could possibly cause bodily injury or death.

CAUTION This calls attention to a procedure, practice, or condition that could possibly cause damage to equipment or permanent loss of data.



1.2 PicoBrite safety notice

WARNINGS

This product must be electrically earthed during operation. The IEC mains

power inlet includes a protective conductor terminal that must be connected to

earth.

Live and Neutral fusing is implemented on this product. Both fuses are located

adjacent to the IEC mains power inlet. Fuse ratings are listed in Specifications.

If this product is used in a manner not specified by the manufacturer then any

safety protection provided by the product may be impaired.

The PicoBrite controller and sources have no user serviceable parts. Please

contact HORIBA Scientific if this product requires servicing. Do not attempt to

open the PicoBrite sources. Opening the PicoBrite sources will cause

malfunction and will invalidate your warranty.

This product should be operated only by personnel who have read and

understood this user guide.

Do not use the product if any cables connected to it are damaged or appear

worn; disconnect from the mains power supply immediately and contact

HORIBA Scientific for advice.

WARNING – LASER HAZARD

Some PicoBrite excitation sources generate laser light or ultraviolet radiation.

Do not look directly at the beam and always observe appropriate safety

procedures. Each PicoBrite source is marked with a safety label indicating the

LED or laser diode classification. These classifications are listed by model in

the following section for reference.

PicoBrite sources are intended for spectroscopic applications and as such

should be incorporated into an enclosed optical system during normal

operation.

If in doubt please contact your organisation’s laser or laboratory safety officer.



CAUTION

PicoBrite heads are designed so that they may be swapped while the

controller is switched on, however to minimise the risk of damage to the

PicoBrite or related equipment it is recommended that the controller module is

switched off while connecting or disconnecting power and signal cables..

1.2.1 PicoBrite classifications and safety considerations

Table 1: Laser safety classifications of PicoBrite heads

Attached label Classification Model numbers

CLASS 3R LASER PRODUCT

PicoBrite-405L PicoBrite-440L PicoBrite-470L


CLASS 3B LASER PRODUCT


CLASS 1 LED PRODUCT PicoBrite-460 PicoBrite-490 PicoBrite-560

PicoBrite-590 PicoBrite-605 PicoBrite-625

CLASS 3B LED PRODUCT

PicoBrite-260 PicoBrite-280 PicoBrite-295

PicoBrite-340 PicoBrite-370

i. Laser safety officer

A laser safety officer must be appointed wherever Class 3R laser heads emitting energy outside of the

400 nm to 700 nm wavelength range or Class 3B laser heads are operated. The laser safety officer

should review this user guide and designate the appropriate safety controls to be implemented.

ii. Enclosed system operation

Class 3B laser heads should, wherever practicable, be used inside a protective enclosure or as part of

an enclosed optical system. Warning labels should be placed upon removable parts of protective

enclosures.



iii. Modification

Modification of the PicoBrite controller or heads invalidates their warranty and may render them

inoperative and/or dangerous. If the product is modified or used in a manner that is not intended or

described in this user guide then it is the user’s responsibility to reclassify and re-label the product

appropriately

iv. Remote interlock/signage

The remote interlock connector (see section 4.2.2) must be used when Class 3B heads are operated.

This should be connected to an emergency master disconnect interlock or to room or protective

enclosure interlocks.

The entrances to areas or protective enclosures containing Class 3B laser heads should be posted with

appropriate warning signs.

v. Keyswitch

All PicoBrite heads, and in particular Class 3B laser heads, should be protected against unauthorized

use by removal of the key from the key control (see section 4.2.1)

vi. Beam stop or attenuator

The inadvertent exposure of bystanders to laser radiation from Class 3B laser heads should be

prevented by the use of a beam attenuator or beam stop. This must be physically attached to the laser

head directly in front of the emission aperture, unless the laser head is mechanically attached to a closed

optical system or protective enclosure.

Class 3B PicoBrite laser heads are supplied with the PicoBrite-BS beam stop attached. Do not remove

the beam stop. Heads supplied with the fibre launcher attached do not require the beam stop due to the

divergence of the beam emitted from the launcher. However, if the launcher is removed from the head

then the beam stop must be used.

vii. Beam paths

The beam emitted by each laser head should be terminated at the end of its useful path by a diffusely

reflecting material of appropriate reflectivity or by an absorber. Open laser beam paths should be

located above or below eye level where practicable.

viii. Specular reflections

Care should be exercised to prevent the unintentional specular reflection of radiation from Class 3R and

Class 3B laser heads. Mirrors, lenses and beam splitters should be rigidly mounted and should be

subject to only controlled movements while the laser is emitting. Special care needs to be taken in the

selection of optical components for Class 3B laser heads and in maintaining the cleanliness of their

surfaces.

ix. Eye protection

Except in installations where laser heads are operated in an enclosed system or protective enclosure,

eye protection should be used in all hazard areas where Class 3R laser products emitting energy outside



of the 400 nm to 700 nm wavelength range or Class 3B lasers heads are in use. Such eye protection

should be designed to provide adequate protection against the specific wavelength in use.



2. Introduction

Thank you for choosing a HORIBA Scientific product.

The PicoBrite is a convenient and high performing source of picosecond and nanosecond optical pulses

spanning wavelengths from the ultraviolet to NIR. Designed as a flexible excitation source for time-

domain fluorescence lifetime measurement, the PicoBrite contains a number of useful features including:

• Compact excitation sources and controller design with true “plug-and-play” flexibility

• Interchangeable, hot-swappable, excitation sources for different wavelengths, samples and applications

• Optical pulse durations from



3. Installation

This section describes how to unpack the PicoBrite and prepare for operation.

3.1 Unpacking

The PicoBrite is a sensitive optoelectronic instrument and should be handled with care at all times.

The PicoBrite is supplied in a carton that has been custom designed to protect its contents. Inspect the

outside of the carton for signs of rough handling and report any damage to HORIBA Scientific

immediately. Do not use the PicoBrite if you suspect any component may have been damaged during

shipping.

We recommend that you retain the carton in case you need to transport the PicoBrite or return it to

HORIBA Scientific for servicing.

The carton will contain one or more of the following items:

• PicoBrite controller module. This is a light grey module containing the power supply and control circuits required for the operation of each PicoBrite excitation source

• Keys (2)*

• Interlock connector*

• PicoBrite drive cable for connecting the laser and LED heads to the controller module*

• Synchronization cable (1m)*

• IEC mains cable

• USB cable*

• PicoBrite head(s); these contain a selected light-emitting diode or laser diode, built-in optics, and a dedicated pulser circuit

• Lens adjustment tool*

• This user guide (containing CD-ROM)

Carefully unpack each component and identify it according to the list above*.

* Please note the items marked with an asterisk are located in the compartment underneath the controller module.



3.2 PicoBrite head

A typical PicoBrite head is shown in figure 3.4. Each head contains a selected light-emitting diode (LED)

or laser diode (LD) emitter, an impulse generator and control circuit, collection optics, and a 35mm

bayonet-style coupling. Class 3B laser heads (without fibre launcher) are also fitted with a beam stop1.

Each model is identifiable by an explanatory label affixed to the top surface; this label contains the

laser/LED classification notice as shown in figure 3.2.

Figure 3.1: PicoBrite head Left – without fibre launcher adapter Right – with optional fibre launcher adapter attached

Figure 3.2: Typical explanatory label for PicoBrite head

1 When the lever is in position “1” the stop is removed from the beam path and light may be emitted from the head. When the lever is in the “0” position the stop prevents the head from emitting light.



Note that the location of the head’s optical aperture is indicated at the top of the label. Indicators are

provided to notify when the head is ready to be operated (Emission Ready) and when it is operating

(Emission On)2.

The output characteristics (wavelength range, beam profile, focus position etc.) are different for each

model of PicoBrite head. The explanatory label states the peak wavelength of the diode as measured in

the factory3.

WARNING

Under no circumstances should the explanatory label containing the

classification notice be removed from the PicoBrite head. Removing the

explanatory label will invalidate your warranty.

Laser heads are factory aligned to emit a collimated beam with plane of polarisation that is nominally

perpendicular to the plane of the explanatory label. LED heads are unpolarised and are typically aligned

to provide a focus within a few centimetres from the edge of the bayonet coupling. The divergence may

be adjusted by moving the lens inside the head using the focus adjustment tool provided with the

controller module; rotating the lens mount clockwise or anti-clockwise moves the lens closer to or further

away from the LED junction.

Depending on the application, it is recommended to use some method of spectral filtering with the

PicoBrite heads. LED heads in particular may emit over a wide spectral range and the use of a

monochromator or band pass filter may be beneficial. HORIBA Scientific manufactures a range of

monochromators that are compatible with the PicoBrite heads (FluoroCube/5000M is recommended),

and a list of available filters is provided in Appendix A.

PicoBrite heads may be connected to your optical system by the following means:

• Mechanical attachment to an enclosed optical system via the 35mm bayonet coupling, or

• Mounting on a pillar for free space operation e.g. on an optical bench or breadboard4, or

• Via an optical fibre e.g. to a confocal microscope (requires “–F” model head – see section 3.2.3).

2 Note that the colour of the indicators varies from model to model. This is to ensure that the indicators remain visible if laser safety eyewear is used. For example, on red laser heads the indicators are both green.

3 The peak wavelength depends on the diode temperature and the operating mode (i.e. pulsed or cw). Unless otherwise stated the value on the label has been measured in pulsed mode at a temperature of 20°C.

4 Class 3B lasers must be equipped with a beam stop before these may be operated outwith an enclosed optical system.



3.2.1 Bayonet coupling

The 35mm bayonet mounting simplifies the mechanical requirements for coupling the PicoBrite heads to

an enclosed optical system, as shown in Figure 3.3. A simple arrangement featuring a 35.1mm hole with

40.2mm counter bore and M3 set screw will make a suitable mounting flange. Figure 3.4 shows the

coupling flange used to mount a PicoBrite excitation source onto the entrance port of a FluoroCube

monochromator. This drawing may be used as the basis for your own flange. Note that the 40.2mm

counter bore helps achieve a light-excluding seal, while the M3 set screw mates with the groove on the

head’s bayonet mounting to achieve a fast connection.

One advantage of this type of mounting over alternative methods is that the laser source may be freely

rotated to alter the beam’s angle of polarisation e.g. 0°, “magic angle”, or 90°.

Diode Mount

Polariser Mount

Sample Chamber Flangeand Lens Mount

Lens and Filter Holder

Lens Spacing Ring

Polariser Holder

SAMPLE

InterlockedSample Chamber

LASER BEAM

LED BEAM

PicoBrite Head

Figure 3.3: Typical application showing a PicoBrite source attached to an enclosed optical

system - in this case an HORIBA Scientific FluoroCube system with interlocked sample chamber.

Schematic drawing showing beam paths for both LED and laser diode sources (side view)

3.2.2 Pillar mounting

The underside of each PicoBrite head contains two M4 tapped holes that may be used with metric pillars

(or posts) available from optical equipment supplier catalogues.

WARNING: Class 3B laser heads may be operated only as part of an enclosed optical system. A beam

stop must be attached to Class 3B laser heads before they may be used in free space. Contact HORIBA

Scientific or your laser safety officer for advice in this situation.



ø 40.2+0.1

-0.0 ø

35.1 +

0.1

-0.0

M3 std tapped hole

O-ring groove

3.6+0.1

-0.0

6.6+0.1

-0.0

9.6+0.1

-0.1

Figure 3.4: Example coupling flange - PicoBrite to FluoroCube/5000M monochromator. 1st

angle projection. A M3 grub screw retains the excitation source by locating the groove on

the 35mm mounting.

3.2.3 Optical fibre coupling

Laser heads may be coupled to single-mode or pseudo single-mode fibres using the optional fibre

launcher adapter as shown in figure 3.1. The fibre launcher is supplied fitted and pre-aligned when a

laser head is ordered using the “-F” suffix. When using the fibre launcher it is recommended to support

the PicoBrite head with a pillar as described in section 3.2.2.

The fibre launcher may be removed at any time if access to the 35mm bayonet coupling is required (for

example to attached the head to an enclosed optical system), however:

• Careful re-alignment of the fibre launcher will be required if the launcher is re-attached to the PicoBrite head. The alignment procedure requires a means of monitoring the intensity of the

light leaving the distal end of the fibre e.g. an optical power meter. See Appendix B for more

information.

• Removing the fibre launcher may affect the classification of your laser head because the beam will change from highly diverging to collimated. Please consult HORIBA Scientific or your laser

safety officer if you require guidance.



Figure 3.5: Controller module – front panel layout

Figure 3.6: Controller module – rear panel layout

3.3 PicoBrite controller

To prepare the controller module for initial use, first check that the mains voltage indicated on the rear

panel is appropriate for your local mains voltage supply. Two voltage settings are possible: 110V/120V

and 220V/240V. Although the controller module contains an auto ranging power supply, it must be fitted

with fuses that are rated appropriately for your mains voltage supply to ensure compliance with

applicable safety standards. Appropriate ratings are stated on the rear panel of the module as shown in

figure 3.6 and also in the Specifications section of this user guide. Please contact HORIBA Scientific for

instructions on how to change the fuses if necessary.



Place the controller module on a flat surface as close to your measurement system as possible. Avoid

positioning the module in a location where:

• It is difficult to access the power switch on the rear panel

• Ventilation to the fan on the rear panel is restricted

• There is a risk of spillage of water or other liquids

Connect the mains power cable between the controller module and an earthed (three pin) mains outlet.

CAUTION

PicoBrite heads are designed so that they may be swapped while the controller

is switched on, however to minimise the risk of damage to the PicoBrite or

related equipment it is recommended that the controller module is switched off

while connecting or disconnecting power and signal cables.

Connect the Drive cable between the PicoBrite Drive socket on the rear of the controller and a PicoBrite

head. For initial tests, choose a PicoBrite head that emits at a visible wavelength if available. The plugs

at each end of the Drive cable are self-latching types and the vertical orientation is indicated by a red dot

or line. Ensure that the red indication on the plug is aligned with the corresponding indication on the

socket to avoid damaging the pins inside the plug. Ensure the PicoBrite head is positioned such that the

aperture is pointing away from you and the emitted beam will not be hazardous when the head is

switched on.

Insert the Interlock connector into the Interlock socket on the rear of the controller. Again, this is a self-

latching connector and the vertical orientation is indicated by a red dot or line.

Insert one of the two keys into the Emission key switch on the front panel, ensuring that it remains in the

vertical position (Standby).

3.4 USB driver and software installation

If desired the PicoBrite controller may be connected to a PC using the upstream USB 2.0 port. This

feature allows the operating parameters to be controlled automatically by HORIBA Scientific software

such as DataStation TCSPC measurement control software (supplied as part of a complete lifetime

system). Additionally, control panel emulator software is supplied with the PicoBrite controller on CD-

ROM and this may be used to control the PicoBrite instead of the LCD display and menu Dial.

For convenience, a downstream USB port is provided for connection to further USB devices.



3.4.1 Driver installation

The driver supplied on the enclosed CD-ROM is compatible with 32-bit versions of Windows XP,

Windows Vista, and Windows 7 operating systems. It is not compatible with 64-bit operating systems at

time of writing. The following procedure refers to installation on Windows XP5.

Connect the USB cable between the PicoBrite USB port (labelled “To Host PC”) and a spare USB 2.06

port on the PC. When the PicoBrite controller is switched on (see Operation section) the following

dialogue box will appear.

Select “No, not this time”

Select “Install from a list or specific location (Advanced)”

5 NOTE: there should be no need to install the driver if a PC has been supplied by HORIBA Scientific as part of a complete system.

In this case the driver may already be installed.

6 Do not connect the PicoBrite controller to a USB 1.x port – erratic operation will result.



Select checkbox for location and navigate to driver folder

containing “picobrite.inf” file on the supplied CD-ROM

(actual path may differ from that shown)

If this message appears select “Continue Anyway”

The PicoBrite USB driver has been installed successfully.

3.4.2 Software installation (control panel emulator)

Once the driver has been successfully installed the control panel emulator software may be installed by

double-clicking the setup.exe in the PicoBrite directory on the enclosed CD-ROM and accepting default

directories.

Use of the software is described in section 4.5.



PicoBrite CD-ROM affixed here.



4. Operating guide

4.1 Front panel controls and indicators

The front panel is organised into three functional groups as shown in figure 4.1:

Figure 4.1: Controller module front panel – functional groups

4.1.1 Key-operated switch and emission indicator

The PicoBrite controller is provided with a key-operated switch in order to prevent the laser and LED

heads being operated by unauthorised personnel. It is recommended that the keys are managed by

your laser safety officer. Two identical keys are provided for your convenience. Replacement keys are

not available. Should both keys be misplaced or damaged it will be necessary to have a new key switch

installed by HORIBA Scientific.

It is strongly recommended to remove the key from the controller when the PicoBrite is not in use. The

key may only be removed when the PicoBrite is in Standby mode; it cannot be removed while the laser

or LED is operating. For safety the PicoBrite heads will not operate if the controller is switched on with

the key in the On position. The key must be returned to the Standby position whenever the controller is

switched on or when a new head is connected (follow the prompts on the display).

The indicator to the right of the key switch will illuminate when the laser or LED is enabled. Note that the

laser or LED is not enabled solely by the operation of the key switch. A number of factors may inhibit

operation and cause the indicator not to illuminate or to flash:

• An open circuit condition on the Interlock circuit e.g. Interlock plug not connected

• Head not initialised correctly e.g. if the head is inserted while the key is in the On position

• Operation of the Slow Gate feature (see 4.2)



4.1.2 LCD display and menu dial

The PicoBrite controller may be operated as a stand-alone module or in conjunction with a PC via the

USB 2.0 interface. In stand-alone operation the menu dial and LCD display are used together to access

the PicoBrite’s settings. A detailed explanation of the menu content is provided in section 4.3.

The display is a 20x2 character backlit type. A setting is provided to switch off the backlight in situations

where low ambient light levels are desirable. The display contrast may also be adjusted to suit the

viewing angle.

The dial is based on a rotary encoder with integral pushbutton. The display will respond each time the

dial is moved clockwise or anticlockwise.

Note the dial and display are disabled when the PicoBrite is operated under computer control. In this

situation the LCD will display:

4.1.3 Status indicators

Indicator (top to bottom) Function (on=illuminated) Colour

CW mode On when PicoBrite operating is set to CW Mode. Off when operating mode is

set to Pulsed.

Blue

Trigger In Functions when external trigger is selected. On when valid signal detected on

Trigger input connector (see 4.2)

Blue

Interlock On when interlock is open circuit Blue

Diode °C Functions with models of PicoBrite head that feature active temperature

control of diode. Indicator iluminates when actual temperature deviates by

more than 1°C from target temperature. Normally illuminates when PicoBrite

head first is first connected or powered on. It is safe to operate head while

indicator is on but critical measurements should not be made until it goes off.

The indicator should be off within five minutes. If this indicator is flashing then

an error condition has been detected and the temperature controller has been

disabled – see troubleshooting section.

Blue

Mains power On when PicoBrite module is powered from the mains. Blue

External Control



4.2 Rear panel connections

Connector

(left to right)

Function Applicable

Mode

Type

Mains power Mains power inlet with on/off switch and fuse tray (live and neutral lines are

fused). WARNING: this equipment must be earthed.

n/a IEC

USB 2.0 To

Host PC

USB connection to host PC (upstream). Port on host PC must be USB 2.0

compliant.

n/a USB Type

B

USB port for connection to other USB devices (downstream). It is

recommended that high bandwidth data acquisition devices be connected

upstream of the PicoBrite.

n/a USB Type

A

Interlock Connection to external interlock circuit. The circuit between the two pins of this

connector must be closed to permit operation of the PicoBite. A connector with

internal loop is supplied. NOTE: this connector is polarised – take care to align

red dots when inserting.

Pulsed,

CW

Lemo

00.302

Trigger input Optional connection to an external trigger source e.g. pulse generator.

Operates when Repetition Rate parameter is set to “Trig. in”. One diode pulse

will be emitted in response to each rising edge detected. Minimum period

allowable between rising edges is 20ns. Input is TTL compatible. Transition

threshold is adjustable in software and is factory set to +1V nominal.

Pulsed Lemo 00

Fast gate input Optional connection facilitates fast gating of diode pulses. Gate may act as

Inhibit or Enable depending to menu selection. Input is TTL compatible.

Transition threshold is adjustable in software and is factory set to +1V nominal.

Pulsed Lemo 00

Slow gate

input

Optional connection facilitates gating of diode emission regardless of operating

mode. Gate may act as Inhibit or Enable depending to menu selection. Input is

TTL compatible. Transition threshold is fixed (+2V nominal). Input may also be

controlled by switching to chassis ground (input high when open circuit, low

when closed circuit).

Pulsed,

CW

Lemo 00

Sync Out

TTL

A TTL-compatible signal generated by the controller module intended for

synchronising external data acquisition modules with the optical pulses

generated by the PicoBrite. One pulse is output for each optical pulse. Output

duration: 10ns nominal at 50MHz and 20ns nominal at 25MHz and below.

Amplitude >2V into 50Ω. For best results terminate in 50Ω. Recommended

cable type: RG174A/U.

Pulsed Lemo 00

Continued…



Connector Function Applicable

Mode

Type

Sync Out NIM A NIM-compatible signal generated by the controller module intended for

synchronising external data acquisition modules with the optical pulses

generated by the PicoBrite. One NIM pulse is output for each optical pulse.

Output duration: 10ns nominal at 50MHz and 20ns nominal at 25MHz and

below. Amplitude -0.8V nominal into 50Ω (must be terminated in 50Ω).

Recommended cable type: RG174A/U. Note all quoted performance

specifications are measured using NIM synchronisation.

Pulsed Lemo 00

PicoBrite Drive Umbilical connection to PicoBrite heads. Use drive cable supplied with

PicoBrite controller. NOTE: this connector is polarised – take care to align red

dots when inserting.

Pulsed,

CW

n/a

Expansion Port Reserved for future application – do not use n/a 25 way D-

type

socket

4.3 Parameter Menu

The following information relates to PicoBrite controllers with firmware release 1.2.5 and is subject to

change. To provide true plug-and-play operation, some operating parameters are stored in non-volatile

memory inside the PicoBrite heads. These are read by the controller when a head is first connected and

are different for each head. Parameters stored in the controller will not change when a head is

connected. The memory location is listed for each parameter (in brackets).

General parameters These options are not specific to either Pulsed or CW operating mode

Parameter

(Memory location) Values Function

Emission mode (Controller)

Pulsed, CW Selects the operating mode. The menu is configured to suit the operating mode; parameters relating solely to CW mode are not displayed in Pulsed mode and vice versa.

Advanced menus (Controller)

On, Off Determines if the menu should list all parameters or an abbreviated set*.

Blank display after* (Controller)

Never, 1 minute, 10 seconds

Switches off the backlight in situations where low ambient light levels are desirable. Default setting is Never (backlight always on). Moving the dial will automatically restore the backlight.

Display contrast* (Controller)

Integer in range 0-20 The display contrast may be adjusted to suit the viewing angle. More extreme settings are programmable using the emulator software.

Diode temperature (Head)

20°C to 40°C in 1°C increments

Option available only when PicoBrite head supports active control of diode temperature. The actual diode temperature is also displayed (no updates).

*Parameters not listed in the abbreviated menu are marked with an asterisk.



Pulsed mode parameters These options are available only when the PicoBrite is operating in Pulsed mode

Parameter


Repetition rate (Controller)

Trig. In, 10kHz*, 20kHz*, 50kHz*, 100kHz*, 250kHz, 500kHz, 1MHz, 2MHz, 4MHz, 5MHz, 8MHz, 10MHz, 16MHz, 20MHz, 25MHz, 50MHz, 80MHz, 100MHz

Selects the repetition rate of the diode. The available settings depend on the head connected to the controller; some heads do not support the higher rates. Note that increasing the repetition rate increases the average power emitted by the diode. Use the lowest repetition rate suitable for the application to preserve the life of the diode. When “Trig. In” is selected the internal clock is bypassed and the repetition rate will be determined by the signal present on the Trigger Input connector.

Bias (Head)

Integer in range 0-255 (model dependent: upper limit may be limited to prevent damage to diode)

This parameter is factory optimised for each PicoBrite head in conjunction with the Amplitude parameter (below) and controls the DC bias voltage applied to the diode. Increasing the bias generally increases the average power and broadens the pulse. If the bias setting is too high for the diode then the optical waveform will not return to zero between pulses. Note that each Repetition Rate setting has its own Bias parameter to permit the optical pulse to be optimised for each repetition rate setting.

Amplitude (Head)

Integer in range 0-255

This parameter is factory optimised for each PicoBrite head in conjunction with the Bias parameter (above) and controls the amplitude of the pulse applied to the diode. Increasing the Amplitude generally increases the average power and broadens the pulse. If the Amplitude setting is too high for the diode then the power may saturate and decrease. Note that each Repetition Rate setting has its own Amplitude parameter to permit the optical pulse to be optimised for each repetition rate setting.

Sync signal* (Controller)

Clock (default), Diode, Delayed clock

This parameter selects the source of the synchronisation pulses available at the NIM and TTL sync outputs. The default choice for most applications is Clock; this option derived the sync pulses from the same clock signal that drives the PicoBrite head. The Delayed clock option also derives from the clock signal but is additionally routed through a digital delay stage (see Sync delay parameter). The Diode option derives the sync pulses from the GHz waveform passing through the diode; this option minimises jitter between the optical pulse and the sync pulse (and may result in a narrowing of the prompt FWHM as measured in TCSPC) however: its use is limited to repetition rates of 25MHz and less, and it requires optimisation of the Sync threshold parameter.

Sync threshold* (Head)

Integer in range 0-255

This parameter operates in conjunction with the Diode sync option and is only available when Diode sync is selected (see Sync signal parameter). This parameter sets the threshold for the circuit inside the head that provides the Diode-derived sync pulse.

Sync delay* (Controller)

-10ns to +10ns in 1ns increments

This parameter operates in conjunction with the Delayed clock option is and is only available when Delayed clock is selected (see Sync signal parameter). This parameter sets the delay between the diode optical pulse and the sync signal. This control is not calibrated and accuracy is ±10% (it is not intended for time base calibration).

Continued…

* Nominal values for ease of display: actual values are 9.77kHz, 19.53kHz, 48.83kHz and 97.66kHz (crystal derived).



Parameter (Memory location)

Values Function

Sync divider* (Controller)

Reference, 1/2, 1/4, 1/8, 1/16

Divides the frequency of the synchronisation pulses available at the NIM and TTL sync outputs by an integer value. The Reference setting leaves the frequency unchanged. This parameter has no effect unless the Delayed Clock option is selected (see sync signal parameter).

Fast gate* (Controller)

Disabled, Lase when Low, Lase when High

The parameter determines the operation of the Fast gate input. If Disabled is selected the signal applied to the input has no effect. If Lase when low is selected the input acts as an inhibit signal i.e. the PicoBrite emits when the input signal is low. If Lase when high is selected the input acts as an enable signal i.e. the PicoBrite emits when the input signal is high.

Slow gate* (Controller)

Disabled, Lase when Low, Lase when High

The parameter determines the operation of the Slow gate input. If Disabled is selected the signal applied to the input has no effect. If Lase when low is selected the input acts as an inhibit signal i.e. the PicoBrite emits when the input signal is low. If Lase when high is selected the input acts as an enable signal i.e. the PicoBrite emits when the input signal is high.

*Parameters not listed in the abbreviated menu are marked with an asterisk.

CW mode parameters These options are available only when the PicoBrite is operating in CW mode

Parameter


CW intensity (Head)

1%, 5%, 10%, 25%, 50%, 75%, 100%

Selects the intensity level of the diode. The 100% level is measured for each head in the factory and the corresponding milliwatt reading is provided on the test sheet provided (the intensity is strongly related to diode temperature). Values



After a few seconds the display will update with the Status Window. The content of the Status Window

depends on the model of PicoBrite head connected, but it should appear similar to:

This confirms that the PicoBrite head has been detected and the operating parameters stored inside the

head have been read by the controller. The Emission Ready indicator on the PicoBrite head should now

be illuminated. The PicoBrite head may now be operated. If it is safe to do so turn the key clockwise to

the On position. The Emission On indicators on the PicoBrite head and controller (next to the key

switch) should now illuminate and the status display should update to:

Verify that laser or LED light is now being emitted from the aperture of the PicoBrite head and that the

nature of this light is consistent with the explanatory label on the head. If the head is Class 3B then

move the beam stop lever at the laser aperture to the “1” position.

The PicoBrite’s operating parameters may be accessed and edited using a single control: the menu Dial.

Rotate the Dial to step through the parameter menu. To select a parameter for editing, depress the Dial

and then rotate the Dial again to change the value. Depress the Dial again to store the new value and

the display will return to the parameter display. To choose another parameter, rotate the Dial again.

(Tip: the display will automatically return to the Status Window if the Dial is not used for approximately

three seconds - if you do not wish to save a changed setting, let the menu return to the Status Window.)

To practice operating the menu Dial, select the Repetition Rate parameter by rotating the Dial then

depressing it when the following window appears:

When the Dial is depressed a “>” symbol appears before the parameter to indicate that the value is now

being edited:

Repetition Rate:

-> 10MHz

Repetition Rate:

10MHz

PB-405L 10MHz

Pulsed Mode: ON

PB-405L 10MHz

Pulsed Mode: OFF



Rotate the Dial to change the value and then depress the Dial once again to store the new value, for

example:

Wait until the Status Window appears. It should now contain the new repetition rate value:

Note that parameters may be edited while the PicoBrite is running and when it is in Standby. For

convenience, the first parameter in the menu is always the one that was most recently edited.

Return the key to the Standby position by turning it anticlockwise. Verify that the Emission On indicator

on the head is no longer illuminated and that no light is being emitted from the aperture.

To verify that the interlock circuit is functioning correctly remove the Interlock connector from the

Interlock socket on the rear panel. The Interlock status indicator on the front panel should illuminate and

the LCD display should now read:

Replace the Interlock connector and switch the controller off using the Mains Power rocker switch. If the

head is Class 3B then set the beam stop lever at the laser aperture to the “0” position to block the beam.

4.5 Software control panel

The software control panel is an alternative means of accessing and modifying the PicoBrite’s operating

parameters. The controller’s LCD display and menu dial are disabled when the software control panel is

in use. In order to use the software control panel the PicoBrite controller must be connected using the

supplied USB cable and the driver and software must be installed as described in section 3.4.

To start the

software control panel

click on the program icon:

When the program initialises it will attempt to connect to the PicoBrite controller. If successful, the main

window will appear containing the current settings of the controller and attached head (see figure 4.2)

and the LCD display on the controller will read:

Interlock

PB-405L 20MHz

Pulsed Mode: ON

Repetition Rate:

20MHz



If the software is unable to connect to the controller during initialisation then the LCD display will remain

unchanged and the software controls will be disabled.

Figure 4.2: Main window.

The parameters shown are the same parameters that are available using the LCD display

and these are described in Section 4.3.

ATTENTION

The software controls adjust the parameters in real time but parameters relating

to the head are not automatically stored in the head’s non-volatile memory

unless the option to permanently remember settings is selected.

Remote Control



5. Maintenance and troubleshooting

5.1 General

The PicoBrite controller contains no user serviceable parts. Please contact HORIBA Scientific if you

require technical support. Contact details for the main offices are provided on the front page of this

guide. Should it be necessary to open the product for inspection purposes, then this must be

undertaken only by personnel qualified to work with electrical equipment that operates at mains voltage.

The product must be disconnected from the mains electricity supply before the top cover is removed.

PicoBrite heads are should never be opened as this will cause damage and/or impair performance.

If either module requires cleaning then please disconnect it from the mains supply and using a soft cloth

(e.g. microfiber type).

5.2 Troubleshooting

5.2.1 LCD Messages

Verify that the Drive cable is connected securely at both

ends.

The controller will not initialise the head until the key is

returned to the Standby position.

Verify that the Interlock plug is correctly inserted.

Where an external interlock loop is in use, check there is

no break in the circuit e.g. open switch.

Switch controller off and wait 30 second before

switching on again. If error code persists then contact

HORIBA Scientific.

Service code XX

Interlock

Return key to

standby

No head detected



5.2.2 Performance issues

i. No light emitted from head

If the head is a Class 3B laser then ensure the beam stop lever at the laser aperture is set to the “1”

position.

ii. Oscillations in TCSPC decay data

Ensure that the PicoBrite repetition rate is longer than the reciprocal of the time range setting on your

Time-to-amplitude converter (TAC). E.g. for a 100ns TAC range setting, the actual (calibrated) time

range may be 110ns, in which case the time between PicoBrite pulses must be >110ns which

corresponds to a repetition rate setting of



6. Specifications

Power requirements 110V 60Hz or 240V 50Hz, 100VA

Fuse Type: 20mm anti-surge on both Live and Neutral

Rating: 2A at 110V 60Hz or 1A at 240V 50Hz

Dimensions* Controller: 234x255x92mm

Head (typical, no accessory): 70x90x40mm

Weight* Controller: 3.1kg

Head (typical, no accessory): 0.5 kg

Operating environment Operating temperature 5˚C to 30˚C

Indoor use only

Compliance

This product complies with:

EN61326-1:1997 + A1:1998 (Equipment for measurement, control &

laboratory use EMC requirements)

EN61000-3-2:2000 (Mains harmonics)

EN61000-3-3:1995 (Voltage fluctuation)

EN61010-1:2001 (Safety requirements for electrical equipment for

measurement, control, and laboratory use Part1: General

requirements)

Classified per IEC 60825-1:2001. Complies with 21 CFR 1040.10 &

1040.11 except for deviations pursuant to Laser Notice No.50 dated

26-July-01

* With no cables attached.



Appendix A: Optical filter ordering information

A range of bandpass filters is available for use HORIBA Scientific’s NanoLED and PicoBrite products.

The following table lists the filters by centre wavelength, FWHM and peak transmission. Unless

otherwise stated filters are supplied in a 35mm bayonet mount as shown.

Figure A1: Mount for optical filters

Order code Centre wavelength Bandwidth (FWHM) Peak transmittance

DF-265-25-20 265nm 25nm 20%

DF-280-25-20 280nm 25nm 20%

DF-310-10-15 310nm 10nm 15%

DF-330-10-20 330nm 10nm 20%

DF-340-10-25 340nm 10nm 25%

DF-360-10-25 360nm 10nm 25%

DF-370-10-25 370nm 10nm 25%

Continued…



Order code Centre wavelength Bandwidth (FWHM) Peak transmittance

DF-375-6-90 375nm 6nm 90%

DF-390-10-30 390nm 10nm 30%

DF-405-10-90 405nm 10nm 90%

DF-440-10-90 440nm 10nm 90%

DF-450-10-45 450nm 10nm 45%

DF-460-10-45 460nm 10nm 45%

DF-470-10-90 470nm 10nm 90%

DF-490-10-45 490nm 10nm 45%

DF-560-10-50 560nm 10nm 50%

DF-590-10-50 590nm 10nm 50%

DF-610-10-50 610nm 10nm 50%

DF-620-10-50 620nm 10nm 50%

DF-640-8-90 640nm 8nm 90%

DF-650-13-90 650nm 13nm 90%

DF-670-10-55 670nm 10nm 55%

DF-740-10-35 740nm 10nm 35%

DF-830-10-50 830nm 10nm 50%

DF-000 Bayonet mount for 12mm (0.5 inch) filter (no filter included)



Appendix B: Fibre coupler adjustment

From time to time, it may become necessary to adjust the PicoBrite fibre coupler to optimise the optical

output power coming out of the fibre.

WARNING

To adjust the fibre coupler, you do not need to disconnect the fibre from the

instrument or from the fibre coupler. If you do, please be aware of the laser

safety risks and adhere to appropriate laser safety procedures. Wear the

appropriate eyewear if necessary.

The fibre coupler features 6 screws at the front:

• 3 hex cap screws to adjust the angular position of the fibre tip

• 3 grub screws to lock the fibre coupler in place

Figure A2: PicoBrite fibre coupler (front view)



To adjust the fibre coupler, the output optical power should be monitored. This can be achieved using

the ratemeter dialog in DataStation or an optical power meter7.

Make sure that the count rate is never too high to avoid any damages on the detector. Use appropriate

ND filters.

Adjustment steps:

1. Unscrew the 3 locking grub screws by about a quarter of a turn.

2. Unscrew/screw the 3 adjustment screws, each at a turn, always in the same order, several times, in order to maximise the optical output power.

3. When the optical output power has reached a maximum, screw the locking screws back in, one at a time, while monitoring the power, to lock the coupler in place. Do not over-tighten the

locking screws.

7 For DynaMyc systems make sure that the instrument is in ‘time-resolved’ mode and that there are no edge or emission filters selected. The pinhole should be slightly opened and a mirror or a somewhat reflective sample should be used.



Notes

1. Safety precautions1.1 Safety symbols1.2 PicoBrite safety notice1.2.1 PicoBrite classifications and safety considerations

2. Introduction3. Installation3.1 Unpacking3.2 PicoBrite head3.2.1 Bayonet coupling3.2.2 Pillar mounting3.2.3 Optical fibre coupling

3.3 PicoBrite controller3.4 USB driver and software installation3.4.1 Driver installation3.4.2 Software installation (control panel emulator)

4. Operating guide4.1 Front panel controls and indicators4.1.1 Key-operated switch and emission indicator4.1.2 LCD display and menu dial4.1.3 Status indicators

4.2 Rear panel connections4.3 Parameter Menu4.4 First time operation4.5 Software control panel

5. Maintenance and troubleshooting5.1 General5.2 Troubleshooting5.2.1 LCD Messages5.2.2 Performance issues

6. SpecificationsAppendix A: Optical filter ordering informationAppendix B: Fibre coupler adjustmentNotes

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