Page 1
i
9
............................................................................................................. i
........................................................................................................... iii
........................................................................................................... iv
........................................................................................................... iv
...........................................................................................................1
1.1 ..................................................................................................1
1.2 ..........................................................................................2
1.3 ..........................................................................................2
..................................................3
2.1 ..............................................................................3
2.2 ............................................................12
.............................................................................21
3.1 ....................................................................................21
3.2 ................................................................................................24
............................................................30
4.1 DNA .......................................................................30
4.2 DNA ...............................................................................................33
4.3 DNA .......................................................................................34
.....................................................................42
Page 2
ii
.........................................................................45
6.1 ................................................45
6.2 ................................................46
6.2.1 ...........................................................46
6.2.2 ...........................................................48
6.2.3 .......................................................49
Page 3
iii
2.1-1 - ........................................ 4
2.1-2 - ................................ 4
2.1-3 - .............................. 11
2.1-4 - .......................... 11
2.2-1 - ...................... 19
2.2-2 - ...................... 20
3.1-1 ................................... 24
3.2-1 (11 -12 ) ..................................... 25
3.2-2 ................................................................... 28
4.1-1 ............................... 31
4.1-2 ....................................... 32
4.2-1 DNA ...................................................................... 34
4.3-1 DNA ................................................................ 34
4.3-2 PCR ............................................................................... 35
4.3-3 PCR ................................................................... 35
4.3-4 NCBI DNA ..................... 36
4.3-5 cag DNA ..................................... 37
4.3-6 DNA PCR ........................................................... 37
4.3-7 PCR .................................................................. 38
4.3-8 ............................................................... 39
4.3-9 ................................................................... 41
Page 5
iv
2.1-1 - .................................... 5
2.1-2 - .................................... 5
2.1-3 ......................................... 7
2.1-4 ......................................... 8
2.1-5 ......................................... 8
2.1-6 ..................................... 9
2.1-7 ......................................... 9
2.1-8 ....................................... 10
2.1-9 ....................................... 10
2.1-10 ....................................... 10
2.2-1 ................................................... 14
2.2-2 ................................................... 15
2.2-3 ................................................... 16
2.2-4 ............................................... 17
2.2-5 ................................................... 17
2.2-6 ................................................... 18
2.2-7 ................................................... 18
2.2-8 ................................................... 19
3.1-1 ....................................................................... 22
3.1-2 ........................................................... 22
3.1-3 ................................................... 23
3.1-4 ................................................... 24
3.2-1 ............................................... 25
3.2-2 100 12 13 ............................................ 26
3.2-3 100 12 13 16 .............................. 26
3.2-4 ............................................... 27
3.2-5 ....................................... 29
3.2-6 ........................................................................... 29
Page 6
v
3.2-7 ................................................................... 29
4.1-1 100 12 15 ........................ 31
4.3-1 DNA ................................................................ 37
5.1-1 GPS .................................. 43
5.1-2 ............................... 43
5.1-3 ................................................................... 44
6.1-1 ....................................... 45
Page 7
1
1.1
9
85
88
-
(Phasianus colchicus)
- -
99
9 20 198
Page 8
2
1.2
100 4 1 106
100 4 1 12 31 100
100 10 1 12 31
1.3
Page 9
3
2.1
- -
- -
(1)
(a)
-
- 1 A1 A2
-
4
A3 -
2 2
A4
1
GPS
(b)
-
A5 -
( )
Page 10
4
A6-1 A6-2
-
A6-3 A6-4
- 10 23
11 25 12 17 2.1-1
2.1-1 - 10 24
11 26 12 16 2.1-2
2.1-2
2.1-1 -
10 23 11 25 12 17
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
0 0 0 0
2.1-2 -
10 24 11 26 12 16
- 0 0 0 0
- 0 0 0 0
0 0 0 0
Page 11
5
2.1-1 -
2.1-2 -
(2)
(a)
Page 12
6
- -
- ( 8 )
( 5 ) ( 2 )
( 2 ) ( 4 ) 21
B1
B3 - ( 3 ) (
2 ) ( 1 ) 6
B2 B4
1
GPS
(b)
- B3
- B4
- B5 B9 -
B10 B12
- 10
23 11 25 12 17 2.1-3
2.1-3 2.1-7 - 10 24
11 26 12 16 2.1-4
2.1-8 2.1-10 -
-
Page 13
7
A-1 A-2
A-3 A-4
A-5 A-6
A-7 A-8
2.1-3
Page 14
8
B-1 B-2
B-3 B-4
B-5
2.1-4
C-1 C-2
2.1-5
Page 15
9
D-1 D-2
2.1-6
E-1 E-2
E-3 E-4
2.1-7
Page 16
10
F-1 F-2
F-3
2.1-8
G-1 G-2
2.1-9
H-1
2.1-10
Page 17
11
2.1-3 -
10 23 11 25 12 17
A-1 0 0 0 0
A-2 4 4 3 4
A-3 0 0 0 0
A-4 0 0 0 0
A-5 0 2 1 2
A-6 0 0 0 0
A-7 0 0 0 0
A-8 0 0 0 0
B-1 0 0 0 0
B-2 0 0 0 0
B-3 0 0 0 0
B-4 0 0 0 0
B-5 0 0 0 0
C-1 0 0 0 0
C-2 0 0 0 0
D-1 0 0 0 0
D-2 0 0 0 0
E-1 0 0 0 0
E-2 0 0 0 0
E-3 0 0 0 0
E-4 0 0 0 0
4 6 4 6
2.1-4 -
10 24 11 26 12 16
F-1 0 0 0 0
F-2 0 0 0 0
F-3 0 0 0 0
G-1 0 0 0 0
G-2 0 0 0 0
H-1 0 0 0 0
0 0 0 0
Page 18
12
2.2
MP3 (play back)
GPS
(1)
-
-
-
5 8 5
5 2 3
23
C1 C3 - 3
4 2
1 7
C2 C4
(2)
6:00 10:00 3:00
6:00 3 6
GPS
3
Goldwave
Page 19
13
(3)
-
100 10 23 11 25 12 17 2.2-1
2.2-5 - 10 24 11 26
12 16 2.2-6 2.2-8
- 2.2-1 -
2.2-2 -
-
Page 20
14
a-1 a-2
a-3 a-4
a-5 a-6
a-7 a-8
2.2-1
Page 21
15
b-1 b-2
b-3 b-4
b-5
2.2-2
Page 22
16
c-1 c-2
c-3 c-4
c-5
2.2-3
Page 23
17
d-1 d-2
2.2-4
e-1 e-2
e-3
2.2-5
Page 24
18
f-1 f-2
f-3 f-4
2.2-6
g-1 g-1
2.2-7
Page 25
19
h-1
2.2-8
2.2-1 -
10 23 11 25 12 17
a-1 0 0 0 0
a-2 4 4 4 4
a-3 0 0 0 0
a-4 0 0 0 0
a-5 0 2 1 2
a-6 0 0 0 0
a-7 0 0 0 0
a-8 0 0 0 0
b-1 0 0 0 0
b-2 0 0 0 0
b-3 0 0 0 0
b-4 0 0 0 0
b-5 0 0 0 0
c-1 0 0 0 0
c-2 0 0 0 0
c-3 0 0 0 0
c-4 0 0 0 0
d-1 0 0 0 0
d-2 0 0 0 0
e-1 0 0 0 0
e-2 0 0 0 0
e-3 0 0 0 0
4 6 5 6
Page 26
20
2.2-2 -
10 24 11 26 12 16
f-1 0 0 0 0
f-2 0 0 0 0
f-3 0 0 0 0
f-4 0 0 0 0
g-1 0 0 0 0
g-2 0 0 0 0
h-1 0 0 0 0
0 0 0 0
Page 27
21
- - 50
-
-
A-2 a-2
3.1
(1)
A-2 a-2
( 3.1-1 3.1-2) 2
1 10 25 11 26
12 14 3
30 1
GardenWatchCamPlayer
11 25 11 26 1
2 7:00
10:00 3.1-3
Page 28
22
3.1-1
12 14
3.1-2
Page 29
23
10/25 11/26
11/26 12/14
3.1-3
(2)
( 3.1-4)
12 12 5
3.1-1
Page 30
24
3.1-4
3.1-1
120.995487° 22.580620° 10 25
120.994869° 22.581310° 11 26
120.995156° 22.580556° 12 12
120.995086° 22.580454° 12 12
120.994404° 22.581464° 12 12
120.994321° 22.581480° 12 12
120.996505° 22.582107° 12 12
120.994339° 22.581501° 12 14
120.996604° 22.582212° 12 14
3.2
4
11 12
3.2-1
Page 31
25
10
( 3.2-1)
10
10
3.2-1 (11 -12 )
11 25 ~11 26
12 12 ~12 16
3.2-1
(1)
Page 32
26
3.2-2 3.2-3
6:00 18:00
1 1
12 13 12 16 9
3.2-2
3.2-2 100 12 13
3.2-3 100 12 13 16
Page 33
27
12 14 12 14
12 16 12 13
12 15 12 14
3.2-4
Page 34
28
3.2-2
12 13 Lanius cristatus 1
Pycnonotus taivanus 6
Streptopelia chinensis 3
12 14
Turdus chrysolaus 1
Egretta intermedia 1
Turdus chrysolaus 3
Acridotheres javanicus 1
Caprimulgus affinis 3
12 15
Lanius schach 1
Turdus chrysolaus 1
Acridotheres javanicus 1
Caprimulgus affinis 1
12 16
Gallinago sp. 1
(2)
( )
50~100 10
17 ( 3.2-5) 12 15 12 16
2 3.2-6 5 6
( 3.2-7)
Page 35
29
3.2-5
3.2-6
3.2-7
Page 37
30
1.
2.
3.
4.
DNA 5.
3
( 1)
( 3) ( 5)
DNA
(mitochondrial)DNA
D-loop (microsatellite) 2
4.1 DNA
3
(1)
12
1
Queen lysis buffer ( 4.1-1)
Page 38
31
4.1-1 100 12 15
(2)
B311
2008 (P.C.
torquatus) B3111
10 3 gDNA
46 ng/ul 286 ng/ul 4.1-1
4.1-1
T4760 C 141 ng/ul M
T4782 C 61 ng/ul
T4820 C 85.5 ng/ul
T4821 C 82.5 ng/ul
T4958 C 286 ng/ul M
T4959 C 65.5 ng/ul
T4960 C 54.5 ng/ul
T4961 C 46 ng/ul
T4962 C 46.5 ng/ul
T5017 C 92.5 ng/ul
T5170 C 50.5 ng/ul
T5171 C 177.5 ng/ul M
T5172 C 84 ng/ul M
(3)
Page 39
32
4
(Phasianus
versicolor) (P. c. karpowi)
(P. c. edzinensis) (P.
c. karpowi) DNA
4.1-2
4.1-2
gDNA
AV. 0168 -20
AV. 0169 -20
AV. 1153 -20
AV. 1156 -20
AV. 1157 -20
AV. 1158 -20
AV. 1159 -20
AV. 1160 -20
AV. 1161 -20
AV. 1164 -20
AV. 1165 -20
AV. 1168 -20
AV. 1296 -20
AV. 1772 -20
AV. 1773 -20
AV. 1831 -20
AV. 1840 -20
AV. 2102 -20
AV. 2169 -20
AV. 2524 -20 295 ng/ul
AV. 2625 -20 346.5 ng/ul
AV. 2784 -20 262.5 ng/ul
AV. 3121 -20 88.5 ng/ul
AV. 3122 -20 123.5 ng/ul
Page 40
33
4.2 DNA
(1) DNA
DNA (1) DNA
(2) DNA
4.2-1
(a) lysis buffer 500 l
100 l 10% SDS
(b) 50 75 l 10mg/ml ProteinaseK
57 2 18
1 2
(c) 13000 rpm 10
(d) 500 l chloroform
(e) 13000 rpm 15 4
(f) d e 3
(g) 0.7 0.8 isopropanol DNA
(h) 13000 rpm 15 4
(i)
(j) 1ml 75%
(k) h i 3
(l) DNA ( )
(m) 50 100 l
(n) 20
14 4
Page 41
34
(Gel electrophoresis) DNA
DNA
4.2-1 DNA
Tris-base 50mM
Na-EDTA 100mM
NaCl 100mM
10% SDS sol 50mg/500ml
Proteinase K 100mg/10ml
Chlorofrom 100%
Isopropanol 100%
75%
4.3 DNA
2 DNA
(microsatellite)DNA DNA Alec J. Jeffrey 1984
D-loop DNA
(Polymerase Chain Reaction
PCR) DNA
DNA 2003 DNA
2 4.3-1
4.3-1 DNA
L16877 GCA TGT ATT ATG TCT AGC AA H1350 CTA CGG CTT GAA AAG CCA TTG TT
Page 42
35
(1) DNA
(a) DNA PCR
proofreading DNA
4.3-2 4.3-3 PCR
1~35
4.3-2 PCR
10XAccuPrimeTM
Pfx Reaction mix 5ul 1X Primer mix (10uM each) 1.5ul 0.3uM each Template DNA (50ng) 1ul 1ng
AccuPrimeTM
Pfx DNA polymerase 0.5ul 1.25 units Distilled water 42ul
Total 50ul
4.3-3 PCR
95 5min 1cycle 95 15sec 35cycles 50 30sec 35cycle 68 90sec 35cycle 68 10min 1cycle 25 10min 1cycle
(b) PCR DNA
1% DNA
gel extraction
kit DNA pGEM R R-T Easy
Vector System DNA
ABI 3730
DNA
(2) DNA
(a)
DNA NCBI
Page 43
36
24
6 ( SR01 FJ221371 SR03 FJ221373 SR08
FJ221378 SR11 FJ221381 SR19 FJ392394 SR20
FJ392395) 6 SR01-F SR03-F SR08-F
SR11-F SR19-F SR20-F 6 5 CGA
DNA
cga ( 4.3-4)
cga ( 4.3-5)
4.3-1
DNA
4.3-4 NCBI DNA
(5’-3’)
SR01 F:hex
aacaaaagtcttcgggtcc (CA)18 122–136
FJ221371 R:tgtgcgtgtgcagaactcc (CA)18 122–136
SR03 F:FAM
tctctgacatatcgcatct (CA)11 299–307
FJ221373 R: atcaagctgcgtatcct (CA)11 299–307
SR08 F: FAM
cgtcacatggctacacctt (CA)15 189–203
FJ221378 R: atcaagctgcgtatcct (CA)15 189–203
SR11 F: FAM
atcaatatggactgctccgt (CA)11AC(CA)3 198–218
FJ221381 R: tccttcaaggccaagtg (CA)11AC(CA)3 198–218
SR13 F: HEX
ttaatacaggcatgtgact (CA)6 151–159
FJ221383 R: cctgctttctctattggt (CA)6 151–159
SR17 F: agaagaccgcattgtt (GA)3AA(GA)5 190–196
FJ221387 R: FAM
agattgcacacgttagcca (GA)3AA(GA)5 190–196
SR18 F: FAM
tatgaaacagacttaatcc (CT)2TCTA5(CAA)7 183–195
FJ221388 R: tgcagcatttgagtaac (CT)2TCTA5(CAA)7 183–195
SR19 F: FAM
catggtatagatgcctgcgta (CA)10TA(CA)4 250–270
FJ392394 R: cgcagcaggaggtccc (CA)10TA(CA)4 250–270
SR20 F: FAM
ccctacaattctgtgattc (CAA)23 169–214
FJ392395 R: agaacaaaatctgctaaaac (CAA)23 169–214
Page 44
37
4.3-5 cag DNA
CAG-tagSR01 cagtcgggcgtcatcaAACAAAAGTCTTCGGGTCC 35 122-136
CAG-tagSR03 cagtcgggcgtcatcaTCTCTGACGTATCGCATCT 35 299-307
CAG-tagSR08 cagtcgggcgtcatcaCGTCACATGGCTACACCTT 35 189-203
CAG-tagSR11 cagtcgggcgtcatcaATCAATATGGACTGCTCCGT 36 198-218
CAG-tagSR19 cagtcgggcgtcatcaCATGGTATAGATGCCTGCGTA 37 250-270
4.3-1 DNA
(b) DNA PCR
PCR
4.3-6 4.3-7
4.3-6 DNA PCR
10XPCR buffer, minus Mg 5.0ul 1X 2.5mM dNTP mixture 1.0ul 0.2mM
DNA DNA
Page 45
38
50mM MgCl2 1.5ul 1.5mM Primer mix (10uM each) 1.0ul 0.2uM each Template DNA (50ng) 1.0ul 1ng Platinum R Taq DNA polymerase 0.2ul 1 units Distilled water 40.3ul
4.3-7 PCR
95 5min 1cycle
95 15sec 35cycles
50 30sec 1cycle
72 30sec 1cycle
72 10min 1cycle
25 10min 1cycle
(3) DNA
DNA 4
1 1 2 DNA
4.3-8 4.3-9
15 T4820
13 T4760
18
Page 46
39
4.3-8
1k1
AGGGGGGGTATACTATGCATAATCGTGCATACATTTATATACCACATATACTATGGTACCGGTACTATATATTATAATCGTACTAAGCCCATTACATGTAGACGGACATTACACCTTAACCCCATTTCTCCCAACGTACAGTCTATGTAATCCCCCAAGACATTAATGCTCTCACCCCAACAAATGATCTACAACTTTCAGGTCACCATACTATGAATGGTTACAGGACATACATGCAATACCAAGTATTCCCCCATTTGGTTATGCTCGACGTACCAGATGGATTTATTGATCGTACACCTCACGAGAGATCACCAACCCCTGCCCGTAATGTACTCCATGACTAGCTTCAGGCCCATTCTTTCCCCCTACACCCCTCGCCCTTCTTGCTCTTTTGCGCCTCTGGTTCCTCGGTCAGGAACATCCCATGCATAACTCCTGAACTCCTCACTTTTCACGAAGTCATCTGTGCATTATTCTCCCCTCTCAAGTCCGTGATCGCGGCATCTTCTCTCTTCATTGCTGTTGGTTCCTTTTTCTCTGGGGCTTCTTCACAGGTTGCCCTTCACAGTGCGGGTGCGGAGTGCTATTCAAGTGAAGCCTGGACTACTCCTGCGTTGCGTCCTATCCTAGTCCTCTCGTGTCCCTCGATGAGACGGTTTGCGTGTATGGGGAATCATCTTGACACTGATGCACTTTGGATCGCATTTGGTTATGGCTCTTCCACCCTCCCGGTTAAATGGTGCTATTTAGTGAATGCTTGTCGGACATATTTTTACAAATTTTCACTTCCTCTATTTTCTCCACAAAACTAGGAGATTTACCACAATTTTTTCTTTAGTTTTTTGTTATTTTTTTTAAAAAAAAAATTAAAAAACTAAATTAACTAAAATTACCGCATAAAAACCCCCAAACCATAGAACGTTTGATTGCGTATATATATATTGTTATATGCAACACTTTTATTAGAGAAACTCCACTACCAAATTCACCT
TTTTAAAAAACAAAATTTTACTAT
Page 47
40
3121 AGGGGGGGTATACTATGTATAATCGTGCATACATTTATATACCACATATATTATGGTACCGGTACTATATATTATAATCGTACTAAGCCCATTACATGTAGACGGACATTACACCTTAACCCCATTTCTCCCAACGTACAATCCATGCAATTTCCCAAGACATTAATGCTCTCACCCCAGCAAATGATCTACAACTTTCAGGTCACCATACTATGAATGGTTACAGGACATACATGCAATACCAAGTATTCCCCCATTTGGTTATGCTCGACGTACCAGATGGATTTATTGATCGTACACCTCACGAGAGATCACCAACCCCTGCCTGTAATGTACTCCATGACTAGCTTCAGGCCCATTCTTTCCCCCTACACCCCTCGCCCCTCTTGCTCTTTTGCGCCTCTGGTTCCTCGGTCAGGAACATCCCATGCATAACTCCTGAACTCCTCACTTTTCACGAAGTCATCTGTGCATTATTCTCCCCTCTCAAGTCCGTGATCGCGGCATCTTCTCTCTTCATTGCTGTTGGTTCCTTTTTCTCTGGGGCTTCTTCACAGGTTGCCCTTCACAGTGCGGGTGCGGAGTGCTATTCAAGTGAAGCCTGGACTACTCCTGCGTTGCGTCCTATCCTAGTCCTCTCGTGTCCCTCGATGAGACGGTTTGCGTGTATGGGGAATCATCTTGACACTGATGCACTTT
GGATCGCATTTGGTTATGGCTCTTCCACCCTCCCGGTTAAATGGTGCTATTTAGTGAATGCTTGTCGGACATATTTTTACAAATTTTCACTTCCTCTATTTTCTCCACAAAACTAGGAGATTTACCACAAATTTTTATTTAGTTTTTT
GTTATTTTTTTAAAAAAAAAAATTAAAAAACTAAATTAACTAAAATTACCGCATAAAAACCCCCAAACCATAAAACGTTTGATTGCGTATATATATATTGTTATATGCAACACTTTTATTAGAGAAACTCCACTACCAAATTCACTTT
TTTAAAAAACAAAATTTTACTAT T4820 AGGGGGGGTATACTATGTATAATCGTGCATACATTTAT
ATACCACATATATTATGGTACCGGTACTATATATTATAATCGTACTAAGCCCATTACATGTAGACGGACATTACACCTTAACCCCATTTCTCCCAACGTACAATCTATGCAATCTCCCAAGACATTAATGCTCTCACCCCAACAAATGATCTACAACTTTCAGGTCACCATACCATGAATGGTTACAGGACATACATGCAATACCAAGTATTCCCCCATTTGGTTATGCTCGACGTACCAGATGGATTTATTGATCGTACACCTCACGAGAGATCACCAACCCCTGCCTGTAATGTACTCCATGACTAGCTTCAGGCCCATTCTTTCCCCCTACACCCCTCGCCCCTCTTGCTCTTTTGCGCCTCTGGTTCCTCGGTCAGGAACATCCCATGCATAACTCCTGAACTCCTCACTTTTCACGAAGTCATCTGTGCATTATTCTCCCCTCTCAAGTCCGTGATCGCGGCATCTTCTCTCTTCATTGCTGTTGGTTCCTTTTTCTCTGGGGCTTCTTCACAGGTTGCCCTTCACAGTGCGGGTGCGGAGTGCTATTCAAGTGAAGCCTGGACTACTCCTGCGTTGCGTCCTATCCTAGTCCTCTCGTGTCCCTCGATGAGACGGTTTGCGTGTATGGGGAATCATCTTGACACTGATGCACTTT
GGATCGCATTTGGTTATGGCTCTTCCACCCTCCCGGTTAAATGGTGCTATTTAGTGAATGCTTGTCGGACATATTTTTACAAATTTTCACTTCCTCTATTTTCTCCACAAAACTAGGAGATTTACCACAAATTTTTATTTAGTTTTTT
GTTATTTTTTTAAAAAAAAAAATTAAAAAACTAAATTAACTAAAATTACCGCATAAAAACCCCCAAACCATAAAACGTTTGATTGCGTATATATATATTGTTATATGCAACACTTTTATTAGAGAAACTCCACTACCAAATTCACTTT
TTTAAAAAACAAAATTTTACTAT
Page 48
41
T4760 AGGGGGGGTATACTATGTATAATCGTGCATACATTTATATACCACATATATTATGGTACCGGTACTATATATTATAATCGTACTAAGCCCATTACATGTAGACGGACATTACACCTTAACCCCATTTCTCCCAACGTACAATCTATGCAATCTCCCAAGACATTAATGCTCTCACCCCAACAAATGATCTACAACTTTCAGGTCACCATACCATGAATGGTTACAGGACATACATGCAATACCAAGTATTCCCCCATTTGGTTATGCTCGACGTACCAGATGGATTTATTGATCGTACACCTCACGAGAGATCACCAACCCCTGCCTGTAATGTACTCCATGACTAGCTTCAGGCCCATTCTTTCCCCCTACACCCCTCGCCCCTCTTGCTCTTTTGCGCCTCTGGTTCCTCGGTCAGGAACATCCCATGCATAACTCCTGAACTCCTCACTTTTCACGAAGTCATCTGTGCATTATTCTCCCCTCTCAAGTCCGTGATCGCGGCATCTTCTCTCTTCATTGCTGTTGGTTCCTTTTTCTCTGGGGCTTCTTCACAGGTTGCCCTTCACAGTGCGGGTGCGGAGTGCTATTCAAGTGAAGCCTGGACTACTCCTGCGTTGCGTCCTATCCTAGTCCTCTCGTGTCCCTCGATGAGACGGTTTGCGTGTATGGGGAATCATCTTGACACTGATGCACTTT
GGATCGCATTTGGTTATGGCTCTTCCACCCTCCCGGTTAAATGGTGCTATTTAGTGAATGCTTGTCGGACATATTTTTACAAATTTTCACTTCCTCTATTTTCTCCACAAAACTAGGAGATTTACCACAAATTTTTATTTAGTTTTTT
GTTATTTTTTTAAAAAAAAAAATTAAAAAACTAAATTAACTAAAATTACCGCATAAAAACCCCCAAACCATAAAACGTTTGATTGCGTATATATATATTGTTATATGCAACACTTTTATTAGAGAAACTCCACTACCAAATTCACTTT
TTTAAAAAACAAAATTTTACTAT
4.3-9
Page 49
42
(habitat selection)
10 11 12
(1)
GPS ( 5.1-1)
excel
A4-1 A4-2
(2)
- ( 5.1-2)
A4-3
4 20
420 5.1-3
100 200
Page 50
43
5.1-1 GPS
5.1-2
Page 51
44
10 11 12 3 11 16
5.1-3
Page 53
45
6.1
100 12 21
6.1-1
4.2
6.1-1
Page 54
46
6.2
6.2.1
(1)
(a)
(b)
(c) DNA
(2)
(a)
(b)
(c)
(3)
(a)
(4)
Page 55
47
(a)
(b) (
)
(c)
(d)
(e)
(f) (
)
(5)
(a)
(v)
(iii)
(6)
(a)
(b)
(c)
(7)
(a)
(b)
( )
(c)
(d) 12
Page 56
48
(e)
(8)
(a) (100) 4 12 3
9
101 3 3
10
6.2.2
(1)
(a)
(2)
(a)
(b)
(3)
(a)
(4)
(a) (
)
(5)
(a)
Page 58
50
(2)
(a)
(
2007)
(b)
(c)
(Barrier Effects),
(d) 466
( )
(e) 68.8%
23.5%
(3)
(a)
(b) 9
(c)
(d)
(e)
Page 59
51
(f) 9
(g)
(h)
(i)
Page 65
A3-1 A3-2
A3-3 A3-4
A3 -
Page 66
A4-1 A4-2
A4 -
A5-1 A5-2
A5-3 A5-4
A5 -
Page 67
A6-1 A6-2
A6-3 A6-4
A6 -
Page 71
B1-1 B1-2
B1-3 B1-4
B1-5
B1 -
Page 72
B2-1 B2-2
B2-3
B2 -
Page 73
B3 -
A-1
A-2
A-3
A-4
A-5
A-6
A-7
A-8
B-1
B-2
B-3
B-4
B-5
C-1
C-2
D-1
D-2
E-1
E-2
E-3
E-4
B4 -
F-1
F-2
F-3
G-1
G-2
H-1
Page 74
1. A-1 2. A-2
3. A-3 4. A-4
5. A-5 6. A-6
7. A-7 8. A-8
B5
Page 75
1. B-1 2. B-2
3. B-3 4. B-4
5. B-5
B6
Page 76
1. C-1 2. C-2
B7
1. D-1 2. D-2
B8
Page 77
E-1 E-2
E-3 E-4
B9
F-1 F-2
F-3
B10
Page 78
G-1 G-2
B11
H-1
B12
Page 81
1 2
3 4
C1-5
C1 -
Page 83
C3 -
a-1
a-2
a-3
a-4
a-5
a-6
a-7
a-8
b-1
b-2
b-3
b-4
b-5
c-1
c-2
c-3
c-4
c-5
d-1
d-2
e-1
e-2
e-3
C4 -
f-1
f-2
f-3 2
f-4
g-1
g-2h-1
Page 87
A4-1
See
Heard
A4-2
Walk
Fly
Rest
Hide
Call
Forage
Page 88
A4-3
X Y(m)
(m)
10 23 120.995972° 22.581013° 4 0 see 240 walk 15:43 8
10 23 120.993848° 22.581384° 1 0 see 20 fly 15:55 12
10 23 120.994104° 22.581707° 1 0 see 30 rest 17:00 13
11 25 120.994380° 22.581602° 0 4 see 60 walk 08:20 12
11 25 120.996047° 22.581494° 2 0 see 220 walk 07:00 10
11 25 120.997360° 22.581270° 1 0 see 360 walk 08:45 4
11 25 120.995886° 22.582039° 2 0 see 180 walk 16:00 10
11 26 120.994744° 22.581314° 0 1 see 100 rest 15:15 10
11 26 120.994199° 22.581585° 0 2 see 40 forage 15:23 26
11 26 120.995176° 22.581152° 2 0 see 150 walk 15:40 22
11 26 120.996199° 22.582032° 1 0 see 210 fly 16:10 17
11 26 120.996290° 22.582309° 0 1 see 200 fly 16:10 20
11 26 120.999425° 22.585803° 0 1 see 420 rest 16:50 12
11 26 120.998880° 22.582732° 0 1 see 420 walk 17:10 13
12 12 120.994688° 22.581892° 0 1 see 80 fly 16:00 12
12 12 120.995432° 22.582611° 2 1 see 120 walk 16:20 13
12 13 120.994679° 22.581591° 0 2 see 100 walk 09:20 12
12 13 120.994679° 22.581591° 1 0 see 100 walk 15:03 12
Page 89
X Y(m)
(m)
12 13 120.997066° 22.581206° 2 0 heard 360 call 16:30 13
12 14 120.994743° 22.581543° 0 1 see 100 fly 07:00 14
12 14 120.994590° 22.581633° 0 1 see 90 fly 07:00 17
12 14 120.996337° 22.581957° 1 0 see 230 walk 07:41 14
12 14 120.997161° 22.580866° 1 0 see 350 walk 08:50 9
12 14 120.995942° 22.582030° 1 0 see 180 walk 10:49 14
12 14 120.997277° 22.581157° 1 0 see 360 walk 13:53 8
12 15 120.994651° 22.581504° 1 0 see 100 fly 11:35 8
12 16 120.996632° 22.581202° 1 0 see 290 walk 14:35 10
12 16 120.996249° 22.581793° 0 1 see 220 walk 10:00 5
12 16 120.994366° 22.581600° 0 1 see 50 fly 11:00 21
12 17 120.996257° 22.582199° 0 1 see 200 hide 10:58 23
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