~ ii~ ~ ~ A Decade of PCR Cold Spring Harbor Laboratory and The Perkin- Elmer Corporation celebrate 10 years of amplifica- tion with a videotape library in which Nobel prize winners Kary Mullis and James Watson and 19 other distinguished scientists review the applications and evolution of the amplification technique hailed as one of the century's most important scientific tools. In 1995, the polymerase chain reaction will be 10 years old. The technique that began as a late-night in- spiration by an unrenowned scientist is now the bed- rock of DNA research, gene discovery, diagnostics development, forensic investigation and environmen- tal science. It has built an industry, provoked a court case, and spawned a dozen books, countless papers and a journal. Along the way, it earned its inventor, Kary Mullis, a Nobel prize~ To mark this anniversary, a conference sponsored by The Perkin-Elmer Corporation was held at Cold Spring Harbor Laboratory in September 1994. Begin- ning with perspectives from James Watson, famed for the discovery of the structure of DNA, and PCR- inventor Kary Mullis, outstanding scientists from a variety of fields reviewed the impact of the technique on their specialties, discussing the present and future applications of PCR technology. A day and a half of wide-ranging, highly il- lustrated talks have been captured in this unique videotape library. The collection will appeal to working scientists from the graduate student level up- wards who apply PCR to problems in human, animal and plant genetics, cell biology, diagnostics, forensic science and molecular evolution. CONTENTS PERSPECTIVES James D. Watson, Cold Spring Harbor Laboratory Kary Mullis, La Jolla AN IN-DEPTH LOOK AT PCR Primers, Oligos and Hybridization Wojciech Rychlik, National Biosciences, Inc. Biology of DNA Polymerases Tom Kunkel, National Institutes of Health PCR Automation and Genotyping Stanley Rose, The Perkin-Eimer Corporation APPLICATIONS OF PCR Human Genome Project Glen A. Evans, University of Texas Human Genetics Henry Erlich, Roche Molecular Systems Molecular Diagnostics Tom Caskey, Baylor College of Medicine Forensic Analysis Bruce Budowle, FBI Academy Gene Evolution/Ancient DNA Svante P~i~ibo, University of Munich Agriculture and the Third World Richard Jefferson, CAMBIA Gene Expression in Single Cells Jim Eberwine, University of Pennsylvania In Situ PCR Ashley Haase, University of Minnesota Combinational Libraries and Rapid Evolution Andrew Ellington, Indiana University THE FUTURE OF PCR Applications of Long Distance PCR Elise Rose, The Perkin-Elmer Corporation PCR Quantitation Francois Ferre, The Immune Response Corporation Analysis of PCR Products in Microchips Stephen Fodor, Affymetrix, Inc. RNA Differential Display Peng Liang, Dana Farber Cancer Institute Representational Difference Analysis Nikolai Lisitsyn, Cold Spring Harbor Laboratory Summary Maynard Olson, University of Washington 1994, 7 edited videotapes (10 hours 15 minutes in total) VHS: ISBN 0-87969-473-4; PAL: ISBN 0-87969-474-2 Price: $300
Transcript
~ ii~ ~ ~
A Decade of PCR Cold Spring Harbor Laboratory and The Perkin-
Elmer Corporation celebrate 10 years of amplifica- tion with a videotape library in which Nobel prize winners Kary Mullis and James Watson and 19 other distinguished scientists review the applications and evolution of the amplification technique hailed as one of the century's most important scientific tools.
In 1995, the polymerase chain reaction will be 10 years old. The technique that began as a late-night in- spiration by an unrenowned scientist is now the bed- rock of DNA research, gene discovery, diagnostics development, forensic investigation and environmen- tal science. It has built an industry, provoked a court case, and spawned a dozen books, countless papers and a journal. Along the way, it earned its inventor, Kary Mullis, a Nobel prize~
To mark this anniversary, a conference sponsored by The Perkin-Elmer Corporation was held at Cold Spring Harbor Laboratory in September 1994. Begin- ning with perspectives from James Watson, famed for the discovery of the structure of DNA, and PCR- inventor Kary Mullis, outstanding scientists from a variety of fields reviewed the impact of the technique on their specialties, discussing the present and future applications of PCR technology.
A day and a half of wide-ranging, highly il- lustrated talks have been captured in this unique videotape library. The collection will appeal to
working scientists from the graduate student level up- wards who apply PCR to problems in human, animal and plant genetics, cell biology, diagnostics, forensic science and molecular evolution.
CONTENTS
PERSPECTIVES James D. Watson, Cold Spring Harbor Laboratory Kary Mullis, La Jolla AN IN-DEPTH LOOK AT PCR Primers, Oligos and Hybridization Wojciech Rychlik, National Biosciences, Inc. Biology of DNA Polymerases Tom Kunkel, National Institutes of Health PCR Automation and Genotyping Stanley Rose, The Perkin-Eimer Corporation APPLICATIONS OF PCR Human Genome Project Glen A. Evans, University of Texas Human Genetics Henry Erlich, Roche Molecular Systems Molecular Diagnostics Tom Caskey, Baylor College of Medicine Forensic Analysis Bruce Budowle, FBI Academy Gene Evolution/Ancient DNA Svante P~i~ibo, University of Munich Agriculture and the Third World Richard Jefferson, CAMBIA Gene Expression in Single Cells Jim Eberwine, University of Pennsylvania In Situ PCR Ashley Haase, University of Minnesota Combinational Libraries and Rapid Evolution Andrew Ellington, Indiana University THE FUTURE OF PCR Applications of Long Distance PCR Elise Rose, The Perkin-Elmer Corporation PCR Quantitation Francois Ferre, The Immune Response Corporation Analysis of PCR Products in Microchips Stephen Fodor, Affymetrix, Inc. RNA Differential Display Peng Liang, Dana Farber Cancer Institute Representational Difference Analysis Nikolai Lisitsyn, Cold Spring Harbor Laboratory Summary Maynard Olson, University of Washington
1994, 7 edited videotapes (10 hours 15 minutes in total) VHS: ISBN 0-87969-473-4; PAL: ISBN 0-87969-474-2 Price: $300
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Impaired Capacity of Cerebellar Patients to Perceive and Learn Two-Dimensional Shapes Based on Kinesthetic Cues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36 Yury Shimansky, Marian Saling, David A. Wunderlich, Vlastislav Bracha, George E. Stelmach, and James R. Bloedel
Lateral Cerebeilar Hemispheres Actively Support Sensory Acquisition and Discrimination Rather Than Motor Control . . . . . . . . . . . . . . . . . . . . . . . . . 49 Lawrence M. Parsons, James M. Bower, Jia-Hong Gao, Jinhu Xiong, Jinqi Li, and Peter T. Fox
Role of Cerebellum in Adapt ive Modification of Reflex Blinks . . . . . . . . . . . . . . . . . . . . . . 77 John J. Pellegrini and Craig Evinger
Single-Unit Evidence for Eye-Blink Conditioning in Cerebellar Cortex is Altered, but Not Eliminated, by lnterpositus Nucleus Lesions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88 Donald B. Katz and Joseph S. Steinmetz
Effect of Varying the Intensity and Train Frequency of Forelimb and Cerebellar Mossy Fiber Conditioned Stimuli on the Latency of Conditioned Eye-Blink Responses in Decerebrate Ferrets . . . . . . . . . . . . . . . . . . . . . . . 105 Piir Svensson, Magnus Ivarsson, and Germund Hesslow
A Model of Pavlovian Eyelid Conditioning Based on the Synaptic Organization of the Cerebellum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 130 Michael D. Mauk and Nelson H. Donegan
Absence of Cerebellar Long-Term Depression in Mice Lacking Neuronal Nitric Oxide Synthase . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 169 Varda Lev-Ram, Zuryash Nebyelul, Mark H. Ellisman, Paul L. Huang, and Roger Y. Tsien
Cover Dissociation of cerebellar attention (yellow and blue) and motor (green and red) activation (yellow and green -- overlap in activation of 3 or more subjects; blue and red = overlap of any 2 subjects). Three- dimensional volume rendering of the cerebellum and brain stem demonstrates that during an attention task, the most common site of activation was in the left superior posterior cerebellum, while during a motor task, the most common site was in the right anterior cerebellum. (For details, see Courchesne and Allen, p. 1; image rendered using VoxelView 2.5.)
The fol lowing articles appea red last m o n t h in t h e first special issue devoted to learning and the cerebel lum, Learning & Memory, vol. 3, n u m b e r 6, March/Apri l 1997
Review
The Cerebellum, LTD, and Memory: Alternative Views Rodolfo Llinfis, Eric J. Lang, and John P. Welsh
Research papers
Preserved Performance by Cerebellar Patients on Tests of Word Generation, Discrimination Learning, and Attention Laura L. Helmuth, Richard B. Ivry, and Naomi Shimizu
A Neural Model of Cerebellar Learning for Arm Movement Control: Cortico-Spino-Cerebellar Dynamics Jose L. Contreras-Vidal, Stephen Grossberg, and Daniel Bullock
Multiple Subclasses of Purkinje Cells in the Primate Floccular Complex Provide Similar Signals to Guide Learning in the Vestibulo-ocular Reflex Jennifer L. Raymond and Stephen G. Lisberger
The Effects of Reversible Inactivation of the Red Nucleus on Learning-Related and Auditory-Evoked Unit Activity in the Pontine Nuclei of Classically Conditioned Rabbits M. Claire Cartford, Elizabeth B. Gohl, Maria Singson, and David G. Lavond
The Learning-Related Activity That Develops in the Pontine Nuclei During Classical Eye-Blink Conditioning Is Dependent on the interpositus Nucleus Robert E. Clark, Elizabeth B. Gohl, and David G. Lavond
Reversible Inactivation of the Cerebellar Interpositus Nucleus Completely Prevents Acquisition of the Classically Conditioned Eye-Blink Response David J. Krupa and Richard F. Thompson
Acquisition of a New-Latency Conditioned Nictitating Membrane Response--Major, But Not Complete, Dependence on Ipsilateral Cerebellum Christopher H. Yeo, Dominic H. Lobo, and Alison Baum
Persistent Phosphorylation Parallels Long-Term Desensitization of Cerebellar Purkinje Cell AMPA-Type Glutamate Receptors Kazutoshi Nakazawa, Sumiko Mikawa, and Masao Ito
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