8/6/2019 A Project on Aloe Vera and Bamboo

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By Shashi Kamal

09BS5991607

A project on Aloe Vera and Bamboo

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Aloe Veray Plant Taxonomy:

y Plant taxonomy classifies aloe vera plant asAloe barbadensis.

y Plant Type:

y Aloe barbadensisgrows as perennial in tropical and sub-tropicalregions, where the leaves of these clump-forming succulents

are evergreen. Typically found in the wild in hot, dryclimates, they're a natural for desert landscaping.

y Sun and Soil Requirements:

y Grow in full sun to partial shade and in a well-drained soil.These succulents are drought tolerant plants onceestablished.

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Chemical composition

y Aloe emodin

y Amino acids

y Anthraquinones

y Barb loiny Carbohydrates:

Polysaccharides, Galactose,

lignin

y Lipids:Gum, saponin, steroids

y Proteolytic enzymes

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Uses of aloe vera

y Aloe vera is used for enhancing skin growth.

y Acid insideAloe vera is used as effective pain killers.

y Antiseptic made fromAloe vera is used to kill mold, bacteria,

funguses and viruses.y Aloe vera uses include help in skin blemishes.

y Acne is treated byAloe vera gel and it's really effective.

y Aloe vera also helps in stopping baldness.

y ImportantAloe vera uses include relief from cuts, bruises and

burns.

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Bambooy Plant Taxonomy:

Agroup of perennial evergreens in the true grass family Poaceae,

subfamily Bambusoideae, tribe Bambuseae.

y

Giant bamboos are the largest members of the grass family.y In bamboo, the internodal regions of the stem are hollow and

the vascular bundles in the cross section are scattered throughout

the stem instead of in a cylindrical arrangement.

The dicotyledonous woody xylem is also absent.

y The absence of secondary growth wood causes the stems

of monocots, even of palms and large bamboos, to be columnar

rather than tapering.

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Chemical compositiony Moisture

y Protein

y Fat

y

Mineralsy Carbohydrate

y Calcium

y Phosphorus

y

Irony Thiamine

y Vitamin C

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Uses of Bambooy Healing power curative properties of Bamboo:-

y Stomach disorder

y Respiratory disorder

y Intestinal worms

y Used in constructiony Bamboo charcoal and vinegar

y Pharmaceutical industry

y Metallurgical industry

y Bamboo for pulp in paper industry

y Toothpick

y In musical instrument and

y Handicraft

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Micro propagation

y Micropropagation is the practice of rapidly multiplying

stock plant material to produce a large number

of progeny plants, using modern plant tissue

culture methods.

y Micropropagation is used to multiply novel plants, such as

those that have been genetically modified or bred through

conventional plant breeding methods. It is also used to

provide a sufficient number of plantlets for planting from a

stock plant which does not produce seeds, or does not

respond well to vegetative reproduction.

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Micropropagating Aloe Vera and

Bamboo

y METHOD

y Source of mother plants BirsaAgricultural University,Kanke, Ranchi

y Collection of ex plant Shoots ofAloe Vera

y Preparation of culture media For in vitro regeneration inAloe Vera ex plants, the MS basal media supplemented withvarious combination of plant growth regulators wereprepared and used.

y Preparation of stock solution Supplements of micronutrient, macro nutrient, organic supplement and ironsource were prepared separately.

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Preparation ofMS Basal media for Aloe

Veray All the stock solution were added to

double distilled water and the volumewas adjusted to 1 litre.

y The Ph of the media was adjustedbetween 5.6 to 5.8 N/10 HCl and

N/10 NaOH was used if necessaryy Agar was added in the media and

homogenized by boiling and stirring.

y The media was poured in the culturebottle

y The bottles were placed in anautoclave and autoclaved at 121degree Celsius and 15lb pressure for16mins.

y Pressure was released from theautoclave and the bottles were kept inthe inoculation chamber.

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Preparation ofMS Basal media for

Bambooy All the constituents except sucrose and agar were

taken with the help pipette or measuring cylinderand kept in a container.

y 30 gm of Sucrose was dissolved in 100 ml ofdistilled water in a conical flask and added in acontainer.

y Required hormones are added in an appropriatemedia.

y The volume was made 1 litre in distilled water

y Ph was 5.6 to 5.8 adjusted by adding N/10 NaOHor N/10HCl with the help ofPh

y After that 7gm of agar was added to the solution,then solution was heated so that the agar dissolved

and became homogenousy The media was poured in a culture bottle.

y The bottles were placed in an autoclave andautoclaved at 121 degree Celsius and 15lbpressure for 16mins.

y Pressure was released from the autoclave and thebottles were kept in the inoculation chamber.

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