A sensor histidine kinase coordinates cell wall architecture

with cell division in Bacillus subtilis

Component annotationPMID:18573169

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2574549/

Simple component annotation

• Figure 1 localizes 2 proteins to the septum of B. subtilis

What proteins?

What GO term?

• “YycG and FtsZ co-localize to the septum”

• Septum can be at the cellular level or at the level of a tissue

• Qualifier: colocalizes_with

Evidence

• Experimental - Yes– Mutant – No– Based on direct

physical interaction – No

– Direct assay – Yes• IDA

Additional annotations

• Figures 2-4 include evidence for annotations that are not allowed in CACAO (protein binding)

• Figure 5 supports annotation of another protein, YycF to GO:0009295 ! Nucleoid via similar localization experiments

• Figure 6 supports other annotations