5th BiotechnologySymposium of theUniversity of Leipzig
May 18/19, 2006
ABSTRACTSEditors:Andrea A. RobitzkiAnnette G. Beck-SickingerPeter SeibelSusanne BrakmannSvenne Eichler
TIB/UB Hannover128 307 927
UNIVERSITAT LEIPZIG
89
CONTENT
FOREWORD 27
PRESENTATIONS 29
1 MOLECULAR BIOLOGY, BIOTECHNOLOGY ANDBIOPHYSICAL APPROACHES TO STUDY PROTEIN STATES 29
1.1 Assembly of type 1 pili in Escherichia coliRudi Glockshuber 30
1.2 Design of immunotoxins using a novel strategy to coupleantibody fragment and toxinMartin Kleinschmidt, Florian Kurschus, Stefan Frost,Dieter Jenne, Rainer Rudolph, Hauke Lilie 31
1.3 Unusual helices in homologous peptides - Beyond the a-helixHans-Jorg Hofmann, Robert Gunther, Carsten Baldauf 32
1.4 Analysis of protein complexes in yeast - A case study ofinteraction of phosphatidylinositol 4-kinase Piklp and 14-3-3proteinsMike Beck, Lars Demmel, Mario Scheme,Anne-Lore Schlaitz, Jan Havlis, Andrej Shevchenko,Christiane Walch-Solimena 33
1.5 Interactions of small peptides (MMP-2 and -9 fragments) withMMP-9 and TIMP-1Christina Weifie, Sven Rothemund, Rolf Gebhardt 34
2 INDUSTRIAL SESSION - BIOANALYTICS 37
2.1 The 3rd generation of protein kinase inhibitorsBert Klebl 38
2.2 Tissue-based, functional high throughput screening for drugdiscovery and drug developmentFrank Striggow 39
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Veterinary diagnostics in the interplay between public combatagainst animal epidemic diseases and keeping of petsTilman Kiihn
From research lab to a high tech organisation - Innovativefluorophores for optical detectionPeter Czerney
A flow cytometry based diagnostic tool for amyloidogenicdiseases - Alzheimer and BSE diagnosisGerald Bohm, Alexander Navarrete Santos,Mingliang Li, Lothar Trieschman, Sandra Torkler,Denny Nowak, Mirjam Gordes, Thomas Lauber,Michael Schaupp
CURRENT NEUROSCIENCES AT THE EDGE BETWEENBIOMEDICINE AND PHYSICS
In situ background correction in fluorescence measurementsDetlev Schild, Tsai-Wen Chen
Quantitative two-photon calcium imaging via fluorescencelifetime analysisChristian Wilms, Jens Eilers
A biomimetic model system shedding light on activelamellipodial biomechanicsBjorn Stuhrmann, Florian Huber, Josef Kas
Knock-down of GFRa4 expression by RNA interference affectsdevelopment of retinal cell types in three dimensional histotypicretinal spheresAndree Rothermel, Katja Volpert, Mirjam Burghardt,Christina Lantzsch, Andrea A. RobHzki, Paul G. Layer
Spotlight on glial cells - Living optical fibers in the vertebrateretinaKristian Franze, Jens Grosche, Serguei N. Skatchkov,Stefan Schinkinger, Detlev Schild, Ortrud Uckermann,Kort Travis, Andreas Reichenbach, Jochen Guck
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4 EVENING LECTURE 53
4.1 Mysterious infections and mass hysteria - Novel entertainmentfor manSucharit Bhakdi 54
POSTERS 57
5 MOLECULAR BIOLOGY, BIOTECHNOLOGY ANDBIOPHYSICAL APPROACHES TO STUDY PROTEIN STATES 57
5.1 Tackling protein-protein interactions with non-natural peptidecaffoldsCarsten Baldauf, Maria Teresa Pisabarro 58
5.2 From genome rearrangements to phylogeniesMatthias Bernt, Daniel Merkle, Martin Middendorf 59
5.3 Production of an esterase from Thermomonospora(Thermobifida) fusca cultivated on cutin, suberin andpoly(ethylene terephthalate) substratesSusan Billig, Wolfgang Zimmermann 60
5.4 Mechanical properties of Rubella virus like particlesChristian Bitterlich, Guido Kohler, Martin Fischlechner,Edwin Donath 61
5.5 Expression, localisation, internalisation and oligomerisation ofY receptors - A fluorescence approach to study protein-protein-interactionsIlka Bohme, Irfan U. Khan, Pascal Dournaud,Annette G. Beck-Sickinger 62
5.6 Attachment of fluorescently labeled DNA to glass slides andsuccessive degradation by exonuclease III - A contribution tosingle molecule sequencingSusanne Brakmann, Katrin Anhalt, Nicky Ehrlich,Christian Hiibner 63
5.7 Directed evolution of low fidelity mutants of HIV-1 reversetranscriptaseSusanne Brakmann, Sascha Nico Stumpp 64
5.8 Investigation of proNPY cleavageKaren Brand, Michaela Bairlein, Diana Lindner,Annette G. Beck-Sickinger 65
5.9 Lipid-anchored oligonucleotides for functionalization ofbiological membranesAndreas Bunge, Anke Kurz, Anne-Katrin Windeck,Maximilian Rost, Wolfgang Flasche, Jurgen Liebscher,Andreas Herrmann, Daniel Hoster 66
5.10 Everything you always wanted to know about chromatinimmunoprecipitation, but were afraid to askHelena Cvijic, Friedemann Horn 67
5.11 Cloning and exploring the human adiponectin receptorsCornelia M. Deckert, Annette G. Beck-Sickinger 68
5.12 Virus engineered colloidal particlesEdwin Donath, Martin Fischlechner, Uta Reibetanz,Jorg Hofmann, Lars Toellner 69
5.13 Heating effects in dual beam laser trapsSusanne Ebert, Kort Travis, Jochen Guck 70
5.14 Indole derivatives as substrates or inhibitors of tryptophan 5-and 6-halogenaseAliz J. Ernyei, Karl-Heinz van Pee 71
5.15 Recombinant expression and structure determination ofphosphodiesterasesRoy Eylenstein, Petra Schafer, Susanne Moschutz,Norbert Strater 72
5.16 Binding site analysis of 3-hydroxybutyrate dehydrogenase from vPseudomonas putida by molecular modelingClaudia Feller, Robert Gunther, Marlis Grunow,Hans-Jorg Hofmann 73
5.17 Model studies for design of effective antisense oligonucleotidesusing fluorescence quenchingYvonne FSrster, Bernd Schwenzer 74
5.1 8 Synthesis and investigation of neuropeptide Y analoguescontaining artificial pyridone dipeptide mimeticsMichael Haack, Peter Tremmel, Sebastian Enck,Armin Geyer, Annette G. Beck-Sickinger 75
5.19 Immobilisation of aldo/keto reductases by protein ligationstrategiesKai Holland-Nell, Annette G. Beck-Sickinger 76
5.20 Solving molecular docking with particle swarm optimizationStefan Janson, Daniel Merkle, Martin Middendorf 77
5.21 Randomly primed rolling-circle amplification - A method forsequence-independent amplification of complete circular DNAvirus genomes from clinical samplesReimar Johne, Daniel Fernandez-de-Luco, Ursula Hofle,Walter Wittig, Hermann Miiller 78
5.22 Comparison of NTPDase 1, 2 and 3 after refolding frombacterial inclusion bodiesMatthias Krause, Norbert Strater 79
5.23 The evolution of the histone methyltransferase gene Su(var)3-9 in metazoans includes a fusion with and a re-fission from afunctionally unrelated geneVeiko KrauB, Anne Fafil, Petra Fiebig, Ina Patties,Heinz Sass 80
5.24 Determination of the oligomeric structure of the bacterialdioxygenase RdpA by small angle X-ray scatteringJorn KrauBe, Norbert Strater 81
5.25 RNase P and MRP as tools in cellular therapyMario Krehan, Astrid Schon 82
5.26 Cooperative binding of TmHU to single ds-DNA as observedby optical tweezersFriedrich Kremer, Mathias Saloitto, Klaus Kroy,Kati Kegler, Christof Gutsche, Jorg Reinmuth,Wiktor Skokow, Marc Struhalla, Claudia Immisch 83
5.27 Analysis of structural elements influencing RNA-cleavage byDicer - Prediction of efficient siRNAAntje Kretzschmar, Jorg Hackermuller,Friedemann Horn, Peter F. Stadler 84
5.28 Characterization of Y-receptor subtypesDiana Lindner, Nicole Merten,Annette G. Beck-Sickinger 85
5.29 Evolutionary conservation and in vitro activity of a splicevariant of the human tRNA nucleotidyltransferaseEsther Lizano, Martin Miiller, Janet Kelso, Mario Mori 86
5.30 Structural analysis of 6-phosphofructokinase (Pfk) from PichiapastorisSascha Marek, Jurgen Kirchberger,E. Bartholomeus Kuttner, Norbert Strater,Torsten Schoneberg 87
5.31 Expression and purification of the ligand binding domain of theperoxisome proliferator-activated receptor aMathias Miiller, Birgit Schneider, Frank Bernhard,Volker Dotsch, Oliver Rau,Manfred Schubert-Zsilavecz, Andrea Sinz 88
5.32 Backbone structure of the membrane binding lipid modifiedC-terminus of the human N-Ras proteinGuido Reuther, Kui-Thong Tan, Julia Kohler,Christine Nowak , Andre Pampel, Klaus Arnold,Jurgen Kuhlmann, Herbert Waldmann, Daniel Huster
5.33 Purification and crystallization of the frons-dienelactonehydrolase from Cupriavidus necatorChristian Roth, Silke Wegener, Michael Schlomann,Norbert Strater
5.34 Modification of amino acid residues in human serum albuminby myeloperoxidasePavel Salavei, Jurgen Arnhold
5.35 Import of peptide conjugated nucleic acids into mitochondriaIngo Schafer, Alexandra Kneissl, Christian Kukat,Peter Seibel
5.36 Hfq influences Escherichia coli CCA-adding enzyme activityMarion Scheibe, Mario Mori
5.37 Characterization of the ligand-binding in the cholecystokinintype A receptor (class A GPCR) expressed in E. coliPeter Schmidt, Cindy Montag, Susann Schimmer,Christin Magg, Annette G. Beck-Sickinger,Rainer Rudolph, Daniel Huster 94
5.38 Folding channels for coarse-grained heteropolymer modelsStefan Schnabel, Michael Bachmann, Wolfhard Janke 95
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5.39 The number of alanines in the N-terminal domain of the poly(A) binding protein PABPN1 dictates its fibrillation propertiesElisabeth Schwarz, Grit Lodderstedt, Mirko Sackewitz,Simone Hess, Gerd Hause, Thomas Scheibel 96
5.40 Protein-ligand interaction of S-adenosyl-homocysteine andmutants of the methyltransferase SU(VAR)3-9Karsten Seeger, Sandro Lein, Gunter Reuter,Stefan Berger 97
5.41 A new fusion protein system for the production of recombinantproteins in the bacterial periplasmBrigitte Sdhling, Ajamaluddin Malik, Rainer Rudolph 98
5.42 Investigation of receptor subtype specificity using chimericY-receptorsJan Stichel, Annette G. Beck-Sickinger 99
5.43 Regulation of the reduced folate carrier (Rfcl) byphenobarbital-type cytochrome P450 inducers andarylhydrocarbons (dioxins)Mareike Strofer, Sandra Halwachs,Carsten Kneuer, Walther Honscha 100
5.44 Radiation induced differential expression of proteins in theintestine of EGFR compromised miceCorinna Sykora, Daniela Volke, Radhika Iyer,JaRhonda Tealer, Howard Thames, Susan C. Evans,Ralf Hoffmann 101
5.45 Arranging the structural interactomeJoan Teyra, Maria Teresa Pisabarro 102
6 INDUSTRIAL SESSION - BIOANALYTICS 105
6.1 High-throughput analysis framework for protein functionalannotationGerd Anders, Maciej Paszkowski-Rogacz,Frank Buchholz, Maria Teresa Pisabarro 106
6.2 Conformational properties of semiconductor-binding syntheticpeptidesMichael Bachmann, Gokhan Gokoglu, Tarik Celik,Wolfhard Janke 107
6.3 Automated four color CD4/CD8 analysis of leukocytes byscanning fluorescence microscopy using quantum dotsJozsef Bocsi, Anja Mittag, Viktor Sebestyen Varga,Bela Molnar, Zsolt Tulassay, Ulrich Sack,Dominik Lenz, Attila Tarnok 108
Residue specific studies of NH exchange rates performed onubiquitinTorsten Brand, Garreth A. Morris, Eurico J. Cabrita,Hans-Jorg Hofmann, Stefan Berger 109
Diffusion NMR in gelling systemsTorsten Brand, Sven Richter, Stefan Berger 110
Apidaecin derivatives and their antimicrobial activityPatricia Czihal, Ute Mdllmann, Jorg Hanrieder,Peter Zipfel, Ralf Hoffmann 111
Biocompatibility of materials for encapsulation of primaryhepatocytesSonja Diekmann, Stephanie SchmHrneier,Attila Tarnok, Jan-Michael Heinrich, Augustinus Bader 112
Synthesis and analysis of Amadori-modified peptidesAndrej Frolov, David Singer, Peter Hoffmann,Ralf Hoffmann 113
Phospholipid changes in cells and body fluids detected by 31PNMR spectroscopy and MALDI-TOF mass spectrometryBeate Fuchs, Jurgen Schiller, Michael Cross,Klaus Arnold 114
6.10 Thermoinduced light emission can image the cellular oxidativestress status and can also detect pharmacologically interestingendocyclic peroxidesMatthias Gilbert, Antje Hutschenreuter,Katarzyna Rataj, Nadine Schmidt, Anika Schumann,Alexander Bauer, Kathrin Sichardt, Jan Hengstler,Karen Nieber, Christian Wilhelm 115
6.11 Ultraslow molecular dynamics of constrained fluids studied byNMR and MRIFarida Grinberg 116
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6.12 Comparison of solid-state NMR and established methods forthe characterization of tissue engineered cartilageMarcus Haberhauer, Goran Zernia, Andrea Deiwick,Claudia Schnepp, Daniel Huster, Augustinus Bader 117
6.13 Analysis of metabolism processes by microorganisms insmall-scale fermentersSigrun Herrmann, Frank Berthold, Joachim Harmel,Karl Held, Regina Hiittl, Peter Klare, Winfried Vonau,Gerhard Wolf 118
6.14 Probing laminin self-interaction by chemical cross-linking andESI-FTICR mass spectrometryStefan Kalkhof, Sebastian Haehn, Christian Ihling,Neil Smyth, Andrea Sinz 119
6.15 PrimExtend - A software for multiplex genotyping primerdesign for mass spectrometry based assaysHolger Kirsten, Steffen Dienst, Peter Ahnert 120
6.16 Introducing IZKF Core facility "DNA technologies"Knut Krohn, Petra Siiptitz, Birgit Thiele, Kathleen Stein 121
6.17 Application of PFG NMR method to study the influence of thesurface of nanoporouse crystal on molecular transportMargarita Krutyeva, Jorg Karger, Sergey Vasenkov 122
6.1 8 Action of methotrexate in various models of RA and MS isdependent on T-cell activationFranziska Lange, Estelle Bajtner, Carola Rintisch,Kutty Selva Nandakumar, Ulrich Sack,Rikard Holmdahl , 123
6.19 Separation of hydroxyproline-isomers and -epimersTobias Langrock, Ralf Hoffmann 124
6.20 Rational design of inhibitors of human phospholipase A2 asanti-inflammatory therapeuticsJens Lattig, Markus Bohl, Herwig Gutzeit,Petra Fischer, Peter Metz, Maria Teresa Pisabarro 125
6.21 AFM in liquid environment simultaneous to advanced opticalmicroscopyChristian Lobbe, Torsten Jahnke, Gerd Behme 126
6.22 IZKF Leipzig Core Unit "Fluorescence-technology/Cytometry"Andreas Losche, Viola Dobel, Attila Tarnok,Ulrich Sack 127
6.23 Novel enzymes for bleaching processesAnke Matura, Julia Schuckel, Monique Thorke,Karl-Heinz van Pee 128
6.24 Nucleic acid immobilization and hybridization on medicaltitanium alloy surfaces - Influences of strand design andimmobilization conditionsJan Michael, Rene Beutner, Ina Israel,Dieter Scharnweber, Hartmut Worch,Bernd Schwenzer 129
6.25 Recombinant antibody fragments to human CD4 expressedin transgenic tobacco induces regulatory properties in naiveCD4+CD45RA+T-cellsDirk Mischke, Ullrich Pigla, Sunna Hauschildt,Frank Emmrich, Jorg Bohme, Rene Brecht,Riidiger Laub 130
6.26 Hyperchromatic cytometry using laser scanning cytometryAnja MHtag, Dominik Lenz, Andreas O.H. Gerstner,Attila Tarnok 131
6.27 Effect of ethanol on the synthesis of large-ring cyclodextrins bya cyclodextrin glucanotransferase from Bacillus sp. BT3-2Mohd Noriznan Mokhtar, Qingsheng Qi ,Wolfgang Zimmermann 132
6.28 An impedimetric approach for non invasive high throughputscreening of cellular properties via 96-well-multielectrode v
arraysJana Miiller, Andree Rothermel, Andrea A. Robitzki 133
6.29 Visualization of cell penetrating peptides with theLumio®-SystemInes Neundorf, Anita Biittner, Robert Rennert,Annette G. Beck-Sickinger 134
6.30 Screening of fungal isolates for production of cutinolyticesterases and their application for polyester fiberThidarat Nimchua, Hunsa Punnapayak,Wolfgang Zimmermann 135
6.31 NMR-spectroscopic investigation of phosphopeptidesChristian Raeck, Stefan Berger 136
6.32 Specific and unspecific transport of anti-tumour therapeuticsinside tumour cells by using carrier peptidesRobert Rennert, Ines Neundorf, Oliver Reiser,Annette G. Beck-Sickinger 137
6.33 Cooperative binding of TmHU to single ds-DNA as observedby optical tweezersMathias Salomo, Klaus Kroy, Kati Kegler, ChristofGutsche, Marc Struhalla, Claudia Immisch,Friedrich Kremer 138
6.34 Solid state NMR characterization of the putative membraneanchor of TWD1 from Arabidopsis thalianaHolger A. Scheldt, Alexander Vogel,Andreas Eckhoff, Bernd W. Konig, Daniel Huster 139
6.35 One step 18F-fluoroalkylation und -arylation of carbonylcompounds by reductive couplingMatthias Scheunemann, Uta Funke, Hongmei Jia,Steffen Fischer, Jorg Steinbach 140
6.36 Visualization of proteomic data with fuzzy-labelled selforganizing mapsFrank-Michael Schleif, Thomas Villmann,Barbara Hammer 141
6.37 Plant stem cells for the production of pharmaceutical activecompoundsDiana Schmidt, Andre Gerth, Dirk Wilken 142
6.38 Detection of Exonuclease III activity in vivo and its applicabilityas a selective screening methodRamona Schmiedel, Sven Pfeifer,Thomas Greiner-Stdffele 143
6.39 Chemical cross-linking and mass spectrometry for structuralmodeling of the annexin A2 /p l 1 heterotetramerDaniela M. Schulz, Andreas Schmidt, Olaf Zschornig,Stefan Kalkhof, Christian Ihling, Andrea Sinz 144
6.40 Interactions of valerian extracts with adenosine A,- andGABAA-receptorsKathrin Sichardt, Zacharie Vissiennon,Axel Brattstrom, Karen Nieber 145
6.41 Monitoring the enzymatic degradation of thioflavin-containingnanoparticles in vitro and in vivoThomas Siegemund, Bernd-Reiner Paulke,Herbert Schmiedel, Natalie Bordag, Steffen Lindert,Anke Hoffmann, Tibor Harkany, Heikki Tanila,Johannes Kacza, Wolfgang Hartig 146
6.42 Phosphorylation dependent antibodies for detection ofAlzheimer's disease specific epitopsDavid Singer, Jorg Lehmann, Katja Hanisch,Wolfgang Hartig, Ralf Hoffmann 147
6.43 Mapping the calcium-free calmodulin/adenylyl cyclase 8complex using isotope-labeled cross-linkers and FTICR MSAndrea Sinz, Christian Ihling, Andreas Schmidt,Stefan Kalkhof, Karl Mechtler, Michael Haack,Daniela M. Schulz, Dermot M. F. Cooper,Annette G. Beck-Sickinger 148
6.44 HPLC in Huntington diseases diagnosticsMarzena Skrzypczak, Wolfram Heinritz,Anna M. Schulz, Ursula G. Froster 149
6.45 Quantification of the a4p2 nicotinic acetylcholine receptorligand 2-['8F]Fluoro-A-85380 in blood during human PETstudiesDietlind Sorger, Georg Becker, Marianne Patt,Andreas Schildan, Kai Kendziorra, Philipp Meyer,Osama Sabri 150
6.46 Analysis of new peptides with antimicrobial activity by massspectrometry v
Christin Stegemann, Alexander Kolobov,Vladimir Kokryakov, Ralf Hoffmann 151
6.47 Dynamics of a lipid-modified Ras peptide in membranesinvestigated by 2H solid-state NMR relaxation and moleculardynamics simulationAlexander Vogel, Catherine P. Katzka,Herbert Waldmann, Klaus Arnold, Scott E. Feller,Michael F. Brown, Daniel Huster 152
6.48 Glycosylation in bovine TauDaniela Volke, Ralf Hoffmann 153
6.49 Joint effort - A candidate gene association study using familytrios based on results of dense genome-wide linkage analysisof Rheumatoid ArthritisJana WeiflfuB, Holger Kirsten, Grit Wolfram,Peter Ahnert 154
6.50 Applications of MALDI-TOF mass spectrometry and 31P NMRspectroscopy lipid researchOlaf Zschornig, Rosmarie SOB, Beate Fuchs,Matthias Muller, Klaus Arnold, Jurgen Schiller 155
6.51 Synthesis and evaluation of Ga-68 labeled Y2-selective peptidesfor in vivo receptor targeting using small animal PETDenise Zwanziger, Irfan U. Khan, Jorn Schlesinger,Ralf Bergmann, Annette G. Beck-Sickinger 156
7 CURRENT NEUROSCIENCES AT THE EDGE BETWEENBIOMEDICINE AND PHYSICS 159
7.1 Distinct effects by interleukin (IL)-l 2 and IL-23 in immunity toCryptococcus neoformans infectionGottfried Alber, Melanie A. Kleinschek, Uwe Muller,Scott J. Brodie, Werner Stenzel, Gabriele Kohler,Wendy M. Blumenschein, Reinhard K. Straubinger,Terrill McClanahan, Robert A. Kastelein 160
7.2 Synoptic stimulation paradigms - Physiological patterns vs.physiologists' patternsMuriel Baum, Jens Eilers 161
7.3 Formation of multicellular hepatic spheroidsDoreen Beck, Stephanie Schmitmeier,Sonja Diekmann, Christoph Mohr, Augustinus Bader 162
7.4 Measurement of the nicotinic acetylcholine receptor availabilitywith PET and reference tissue approachesGeorg Becker, Marianne Patt, Dietlind Sorger,Andreas Schildan, Philipp Meyer, Kai Kendziorra,Osama Sabri 163
7.5 p53 functional domains responsible for repression of cell cyclepromotersLevin Bohlig, Karen Rother, Kurt Engeland 164
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7.6 Cholinergic activation of flight motor activity in the locust,Schistocerca gregariaEdgar Buhl, Paul A. Stevenson, Jan Rillich,Klaus Schildberger
7.7 Simulation of rotating tissue cultures in biotechnologyRaymond Chan, Wolfgang Hackbusch,Christina Lantzsch, Andrea A. Robitzki,Andree Rothermel, Angela Stevens
P2Y, receptor-deficient mice have altered responses tomotivation-related stimuliVanessa Drendel, Cornelius Donat, Holger Kittner,Peter Hies, Ute Kriigel
Do perineuronal nets influence the intraneuronal ironconcentration?Anja Fiedler, Markus Morawski, Tilo Reinert,Gert Bruckner, Tilman Butz, Thomas Arendt
7.10 Actin dynamics and cell motilityJan Fuhrmann, Josef Kas, Angela Stevens
7.11 Influence of hyaluronan and Ultraviolet-B on melanoma cellmotilityCarl Gebhardt, Marco Averbeck, Andrea Viertel,Friederike Kauer, Verena Voelcker, Ulf Anderegg,Jan C. Simon
7.12 The role of expression of FLICE and FLICE-inhibitory proteinisoforms in acute myeloid leukaemiaNicole Georgi, Uwe Platzbecker, Christian Thiede,Gerhard Ehninger, Thomas Illmer
7.13 Tau-tracks for APP-vesicles - A connection toAlzheimer's diseaseJens Gerdelmann, Josef Kas, Thomas Arendt
7.14 Activation of postsynaptic P2Y, receptors blocks long-termdepression in layer V pyramidal neurons of the rat medialprefrontal cortexSegundo J. Guzman, Zoltan Gerevich, Peter Hies
7.15 Tissue engineering of cartilage - Biocompatible scaffoldsMarcus Haberhauer, Andrea DeKvick,Claudia Schnepp, Augustinus Bader
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7.16 Modification of CD44 in human melanoma cells - Impact onreceptor-ligand interaction with hyaluronic acid, tumour growthand therapyChristian Haiduk, Sven Rothemund,Thea Eichenberg, Marco Averbeck, Carl Gebhardt,Ulf Anderegg, Jan C. Simon 175
7'.17 Influence of neural activity and pre- and postsynapticmechanisms on APP secretion from organotypic slice cultures ofmouse brainMaike Hartlage-Rubsamen, Nils Brose,Steffen Rofiner 176
7.18 Characterisation of local-field-potentials in the auditorybrainstem using multielectrode arraysMartin Haustein, Thomas J. Reinert, Ivan Milenkovic,Rudolf Rubsamen 177
7.19 Soluble oligomers of (3-amyloid(l-42) and hydrogen peroxideaffect distinct pools of genes in cholinergic SN56 cellsKatrin Heinitz, Regino J. Perez-Polo,Reinhard Schliebs
7.20 Modulation of connexin 43 expression in cultured smoothmuscle cells of the human urinary bladderMarco Heinrich, Jens-Uwe Stolzenburg,Jochen Neuhaus
7.21 Immunomodulation by mercury salts - The role of cellactivation in deviating the Thl/Th2 balanceNasr Hemdan, Frank Emmrich, Ulrich Sack
7.22 Serotonin transporters in obsessive-compulsive disorder asmeasured in v/vowith [nC]DASB PETSwen Hesse, Katarina Stengler-Wenzke,Marianne Patt, Georg Becker, Katharina Grate,Michael Kroll, Henryk Barthel, Philipp Meyer,Anita Seese, Matthias C. Angermeyer, Osama Sabri 181
7.23 Automated analysis and recognition of phases of cell cycle onHep-2 cells by immunofluorescenceNadja Hilger, Rico Hiemann, Ursula Anderer,Ulrich Sack 182
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7.24 Characterisation of microdissected invasive fibroblasts inwell-defined regions of arthritic jointsNadja Hilger, Franziska Lange, Ulrich Sack 183
7.25 Comprehensive cytogenetic characterization of anesthesioneuroblastomaHeidrun Holland, Ronald Koschny, Wolfgang Krupp,Jurgen Meixensberger, Peter Ahnert 184
7.26 PDGF stimulates VEGF production by human retinal pigmentepithelial cellsMargrit Hollborn, Andreas Bringmann,Peter Wiedemann, Leon Kohen 185
7.27 Corpus luteum derived cells form heterospheroidsKatja Hummitzsch, Albert Ricken, Daniel Klofi,Sabine Erdmann, Andree Rothermel,Andrea A. Robhzki, Katharina Spanel-Borowski 186
7.28 Aquaporin-9 expressing amacrine cells in the rat retinalanors landiev, Bernd Biedermann,Andreas Reichenbach, Peter Wiedemann,Andreas Bringmann 187
7.29 Development of a laser-based micro-dissection andmanipulation system for selective and directed positioning ofcells on nano- and microstructured electronic chipsHeinz-Georg Jahnke, Katharina Jahn,Andree Rothermel, Randy Kurz, Winnie Weigel,Maik Schmidt, Bjorn Stuhrmann, Josef Kas,Andrea A. Robitzki 188
7.30 Cerebral nicotinic acetylcholine receptors (nAChRs) in patients v
with Alzheimer's disease (AD) assessed with 2-F18-FA85380(2-FA) and Positron Emission Tomography (PET) - Correlation todementia severityKai Kendziorra, Philipp Meyer, Henrike Wolf,Henryk Barthel, Swen Hesse, Georg Becker,Andreas Schildan, Anita Seese, Dietlind Sorger,Donald Lobsien, Hermann-Josef Gertz, Osama Sabri 189
7.31 Characterization of mesenchymal stem cells under hypoxiaKathleen Kintzel, Manuela PendzKviat,Frank Emmrich, Manja Kamprad 190
7.32 Impedimetric screening of ligand-receptor interactions on celland tissue-based microarraysDaniel Klofi, Maik Schmidt, Annette G. Beck-Sickinger,Andree Rothermel, Andrea A. Robitzki 191
7.33 Borrelia burgdorferi dissemination in the host - Contrasting theeffects of bacterial spreading after intradermal and intravenousinjection of the spirochetes into miceJens Knauer, Silke Schulz, Samiya Al-Robaiy,Reinhard K. Straubinger 192
7.34 Comparison of a 143B rho° cell line devoid of mtDNA with itsparental wild-typeAlexandra Kneissl, Christian Kukat, Ingo Schafer,Peter Seibel 193
7.35 Optical neuron guidance and actin dynamics in growth conemotilityDaniel Koch, Timo Betz, Allen Ehrlicher,Bjorn Stuhrmann, Michael Gogler, Josef Kas 194
7.36 Physiological effects of splice variants of PFKFB3 protein inU87 glioblastoma cellsKristin Kollner, Renate Kessler, Klaus Eschrich 195
7.37 Developmental and amyloid plaque-related changes in cerebralcortical capillaries in transgenic Tg2576 Alzheimer miceElena Kouznetsova, Reinhard Schliebs 196
7.38 Conversion of an acetylcholine receptor to an atropine receptorDoreen Krause, Angela Schulz, Torsten Schoneberg 197
7.39 Dynamics of the mitochondrial single-stranded DNA-bindingprotein (mtSSB) in human cellsChristian Kukat, Alexandra Kneissl, Ingo Schafer,Peter Seibel 198
7.40 Online biomonitoring of chronotropic effects - A novel toolfor sensitive detection of AT, autoimmune antibodies in sera ofpreeclamptic pregnant womenRandy Kurz, Markus Riiffer, Andree Rothermel,Winnie Weigel, Andrea A. Robitzki 199
7.41 Anti-TNF effects on destructive fibroblasts depend onmechanical stressFranziska Lange, Stephan Hait i , Ute Ungethiim,Ralf-Jiirgen Kuban, Stefan Hammerschmidt,Lars Morawietz, Hubert Wirtz, Frank Emmrich,Veit Krenn, Ulrich Sack 200
7.42 Production and regulation of pigment epithelium derived factorby retinal glial (Muller) cellsJohannes Lange, Susanne Jakel, Yousef Yafai,Marie Koch, Wolfram Eichler, Peter Wiedemann,Andreas Reichenbach 201
7.43 A three dimensional retina model system reveals newproperties of the GDNF family ligand persephin during retinaldevelopmentChristina Lantzsch, Andree Rothermel,Andrea A. Robitzki 202
7.44 Glue or goo? Biomechanical properties of neurons and glialcells in the CNSYunbi Lu, Kristian Franze, Christian Steinhauser,Frank Kirchhoff, Hartwig Wolburg, Jochen Guck,Paul Janmey, Josef Kas, Andreas Reichenbach 203
7.45 DNA modification and thyroid mutagenesisJacqueline Maier, Ralf Paschke, Knut Krohn 204
7.46 Nicotinic acetylcholine receptors (a4p2) and their relationshipto cognitive symptoms in Parkinson's disease (PD) - A 2-[18F]F-A-85380-PET (2FA-PET) studyPhilipp Meyer, Kai Kendziorra, Swen Hesse,George Becker, Henryk Barthel, Karl Strecker,Anke Hensel, Florian Wegner, Marianne Patt,Anita Seese, Dietlind Sorger, Donald Lobsien,Hermann-Josef Gertz, Johannes Schwarz,Osama Sabri 205
7.47 Effects of STW 5 (Iberogast®) and STW6 [Iberis amara) ongastrointestinal inflammation in vitroSebastian Michael, Claudia Warstat, Olaf Kelber,Karen Nieber 206
7.48 Purinergic (P2) receptors in spherical bushy cells of the gerbilcochlear nucleusIvan Milenkovic, Rudolf Rubsamen 207
7.49 Quantitative confocal immunofluorescence analysis of receptorexpression in human detrusor and isolated bladder smoothmuscle cellsJochen Neuhaus, Thilo Schwalenberg,Jens-Uwe Stolzenburg 208
7.50 Bioelectrical monitoring of ligand receptor interactions viaimpedance measurements on a novel tumour sensor modelMatthias Nieber, Jana Muller, Peter Wolf,Maik Schmidt, Andree Rothermel,Andrea A. Robitzki 209
7.51 Alterations of Muller glial cells during diabetic retinopathyThomas Pannicke, lanors landiev, Antje Wurm,Ortrud Uckermann, Franziska vom Hagen,Andreas Reichenbach, Peter Wiedemann,Hans-Peter Hammes, Andreas Bringmann 210
7.52 Activated glial cells do not interfere with the developmentof perineuronal nets of extracellular matrix in midbrain sliceculturesSanja Pavlica, Jens Grosche, Gert Bruckner 211
7.53 The role of the Elf-3/TGF-p in the gastro-intestinal tractMartina Protschka, Manfred Blessing 212
7.54 Stroke - An in vitro model of cellular therapyDoreen M. Reich, Susann Hau, Johannes Boltze,Wilf ried Naumann, Man ja Kamprad, Frank Emmrich 213
7.55 Activity-dependent development of perineuronal nets inorganotypic brain slice culturesSabrina Reimers, Maike Harrlage-Riibsamen,Nils Brose, Gert Bruckner, Steffen Rofiner 214
7.56 Complex kinetics of P2X7-dependent single channel currentsThomas G. Riedel, Hja Lozinsky,Giinther Schmalzing, Fritz Markwardt 215
7.57 Ca2+ responses of Muller cells induced by light stimulationof photoreceptor cellsKatja Rillich, Janina Gentsch, Michael Weick,Andreas Bringmann, Andreas Reichenbach 216
7.58 The transcription factor Yin Yang 1 is an activator of BACElexpressionSteffen RoBner, Katrin Nowak , Christine Lange-Dohna,Ulrike Zeitschel, Albrecht Giinther, Bernhard Liischer,Andrea A. Robitzki, Regino J. Perez-Polo 217
7.59 In vitro desensitization of adenosine A, receptors afterexposure to adenosin analoguesCornelia Rufke, Sarah Kiichler, Karl-Norbert Klotz,Karen Nieber 218
7.60 Cryopreservation methodology for long-term freezing of skinconstructs produced in bioreactors under vital conditionsThomas Rupf, Katrin Lorenz, Jurgen Salvetter,Augustinus Bader
7.61 Influence of plasma treated polyurethanes on theelectrophysiological parameters in human macrophagesSilvia Sachse, Sunna Hauschildt, Peter Wiinsche,Gunter Bottcher, Karen Nieber
7.62 LPS stimulates IL-6 expression in human bladder smoothmuscle cellsNadine Schlichting, Andreas Oberbach,Matthias Bluher, Peter Kovacs,Jens-Uwe Stolzenburg, Jochen Neuhaus
7.63 Murine bone marrow-derived dendritic cells are potentproducers of the interleukin-12 cytokine family after in vitroinfection with Salmonella EnteritidisSabine Schoneberger, Nicole Schiitze,Marina A. Freudenberg, Reinhard K. Straubinger,Gottfried Alber
219
220
221
2227.64 Chondrogenesis and proliferative behaviour of ovine
chondrocytes in matrix-induced autologous chondrocytetransplantats and their clinical outcomeRonny Schulz, Matthias Zscharnack, Isabell Hanisch,Michael Geiling, Pierre Hepp, Augustinus Bader 223
7.65 Analyzing the mitochondrial fusion activityTina Stofiel, Christian Kukat, Alexandra Kneissl,Ingo Schafer, Peter Seibel 224
7.66 Temporal properties of inhibition shaping the response ofMNTB principal cellsSandra Tolnai, Bernhard Englitz, Susanne Dehmel,Cornelia Kopp-Scheinpflug, Jurgen Jost,Rudolf Rubsamen 225
7.67 Small fragments of hyaluronic acid induce metalloproteinaseand cytokine upregulation in human melanoma cells - Possiblerole of TLR4 and CD44Verena Voelcker, Carl Gebhardt, Marco Averbeck,Ulf Anderegg, Jan C. Simon 226
7.68 Functional assessment of ryanodine receptor mutations inMalignant hyperthermiaMarkus Wehner, Henrik Riiffert,Claus-Dieter Meinecke, Kerstin Krist,Daniela Geier, Fritjoff Konig, Derk Olthoff 227
7.69 Cellular localisation of glycogen phosphorylase suggests novelroles in retinal developmentWinnie Weigel, Brigitte Pfeiffer-Guglielmi,Bernd Hamprecht, Andrea A. Robitzki,Andree Rothermel 228
7.70 Intraindividual comparison of the osteogenic differentiationpotential of mesenchymal stem cells derived from adult adiposetissue and bone marrowKonstanze Weinzierl, Dirk Halarha,Alexander Burkhardt, Frank Gaunitz,Berhard French 229
7.71 Impedance based monitoring of neuropeptide Y-receptor Y,in mamma carcinoma cells - An in vitro approach for theidentification of cellular targets involved in the development ofbreast cancerPeter Wolf, Claudia Stein, Andree Rothermel,Andrea A. Robitzki 230
7.72 VEGF prevents osmotic glial cell swellingAntje Wurm, Thomas Pannicke, Ortrud Uckermann,Franziska vom Hagen, Peter Wiedemann,Andreas Reichenbach, Andreas Bringmann 231
7.73 Angiostatic guardians of the retina secreted by Muller cellsYousef Yafai, Wolfram Eichler, Johannes Lange,Andreas Reichenbach, Peter Wiedemann 232
7.74 Effect of initial seeding density of mesenchymal stem cells incollagen gel on contraction, proliferation and chondrogenicdifferentiationMatthias Zscharnack, Ronny Schulz, Pierre Hepp,Augustinus Bader 233
EUROPEAN STRATEGY O N LIFE SCIENCES ANDBIOTECHNOLOGY 235
The european strategy on life sciences and biotechnologyGabriele Siiptitz 236
CENTER FOR BIOTECHNOLOGY AND BIOMEDICINE 239
The Center for Biotechnology and Biomedicine (BBZ) -An overview 240
COLLABORATIVE RESEARCH CENTER SFB 6 1 0 245
Collaborative Research Center SFB 610 246
CONTECTOUFE - CONNECTING TECHNOLOGIES TO UFE 249
ConTecToi/fe - Connecting Technologies to LifeThe Life Sciences Cluster in Mitteldeutschland 250
DFG RESEARCH TRAINING SCHOOL "INTERNEURO" 253
DFG Research Training School "InterNeuro" -Interdisciplinary approaches in the cellular neurosciences 254
INDEX 257
