ACQUITY Advanced Polymer Chromatography (APC) Applications Notebook
[ APPLICATION highlight ]
TABLE OF CONTENTS
LIST OF POLYMERS ANALYZED USING ACQUITY APC
Polymer Part Number Page
Poly(styrene) in THF Calibration Curves WA64641 3
Poly(vinylacetate) in THF WA64642 4
Poly(vinyl sulfone) in THF WA64643 5
Poly(methyl methacrylate co ethyl acrylate) in THF WA64644 6
Poly(vinylidene chloride co acrylonitrile co methyl methacrylate) in THF WA64645 7
Polyurethane in THF WA64646 8
Poly(methylmethacrylate co hydroxyethylmethacrylate) in THF WA64647 9
Epoxy Resin in THF WA64648 10
Poly(vinylpyrrolidone co methyl methacrylate) in THF WA64649 11
Poly(methyl methacrylate) in THF WA64650 12
Poly(phenylglycidyl ether) co formaldehyde in THF WA64652 13
Poly(o-cresyl glycidyl ether) co formaldehyde in THF WA64653 14
Poly(phenyl isocyanate) co formaldehyde in THF WA64654 15
Poly(styrene), Low Molecular Weight in THF WA64655 16
Poly(isobutyl methacrylate) in THF WA64656 17
Poly(bisphenol A co epichlorohydrin) in DMF WA64657 18
Poly(vinylacetate) in DMF WA64658 19
Poly(9,9 di-n-octylfluorenyl 2,7-diyl) in Toluene WA64659 20
Poly(styrene) Standards in THF WA64660 21
Poly(dimethyl silicone) in THF WA64711 22
Poly(dimethyl siloxane) in Toluene WA64712 23
Aqueous Analysis of Pullulans WA64661 24
Poly(styrene) in THF Calibration Curves using ACQUITY APC Columns
LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)
Eluent: THF
Detection: UV at 254 nm
Temperature: 30 °C
Flow rate: 0.6 mL/min
Samples: Poly(styrene)
2520000
549000
130000
34800
9130
1250
277000
130000
34800
176009130
3470
66000
9130
3470
1250
266
15700
4920
22801250
682370
162100
1,000
10,000
100,000
1,000,000
10,000,000
0.50
Elution Volume (mL)
Mol
ecul
ar W
eigh
t (D
a)
1.00 1.50 2.00
ACQUITY APC™ 450, 2.5 µm
ACQUITY APC 200, 2.5 µm
ACQUITY APC 125, 2.5 µm
ACQUITY APC 45, 1.7 µm
© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64641
3
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© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64642
4
[ APPLICATION highlight ]
Analysis of Poly(vinylacetate) in THF using ACQUITY APC Columns
LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)
Eluent: THF
Detection: ACQUITY RI
Column: ACQUITY APC™ XT 4.6 x 150 mm x 3 (450Å, 125Å and 45Å in series), part numbers 186007010, 186007000 and 186006995, respectively
Flow rate: 1 mL/min
Temperature: 40 °C
Injection volume: 30 µL
Sample load: 0.2% (w/v)
Sample: Poly(vinylacetate)
µRIU
-2.00
0.00
2.00
4.00
6.00
8.00
10.00
12.00
14.00
Minutes
2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00 4.20 4.40 4.60 4.80 5.00
© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64643
5
[ APPLICATION highlight ]
Analysis of Poly(vinyl sulfone) in THF using ACQUITY APC Columns
LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)
Eluent: THF
Detection: ACQUITY RI
Column: ACQUITY APC™ XT 4.6 x 150 mm x 2 (450Å and 125Å in series), part numbers 186007010 and 186007000, respectively
Temperature: 40 °C
Injection volume: 20 µL
Flow rate: 1 mL/min
Sample load: 0.2% (w/v)
Sample: Poly(sulfone)
-1.00 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00
10.00
0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00
µRIU
Minutes
6
[ APPLICATION highlight ]
Analysis of Poly(methyl methacrylate co ethyl acrylate) in THF using ACQUITY APC Columns
LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)
Eluent: THF
Detection: ACQUITY RI
Column: ACQUITY APC™ XT 4.6 x 150 mm x 2 (450Å and 125Å in series), part numbers 186007010 and 186007000, respectively
Temperature: 40 °C
Flow rate: 1 mL/min
Injection volume: 20 µL
Sample load: 0.2% (w/v)
Sample: Poly(methyl methacrylate co ethyl acrylate)
-2.50 -2.00 -1.50 -1.00 -0.50 0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00
0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00
µRIU
Minutes
© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64644
7
[ APPLICATION highlight ]
Analysis of Poly(vinylidene chloride co acrylonitrile co methyl methacrylate) in THF using ACQUITY APC Columns
LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)
Eluent: THF
Detection: ACQUITY RI
Column: ACQUITY APC™ XT 4.6 x 150 mm x 2 (450Å and 125Å in series), part numbers 186007010 and 186007000, respectively
Temperature: 40 °C
Injection volume: 20 µL
Flow rate: 1 mL/min
Sample load: 0.2% (w/v)
Sample: Poly(vinylidene chloride co acrylonitrile co methyl methacrylate)
µRIU
Minutes
-1.00 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00
10.00
0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00
© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64645
8
[ APPLICATION highlight ]
Analysis of Polyurethane in THF using ACQUITY APC Columns
LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)
Eluent: THF
Detection: ACQUITY RI
Column: ACQUITY APC™ XT 4.6 x 150 mm x 3 (450Å, 125Å and 45Å in series), part numbers 186007010, 186007000 and 186006995, respectively
Flow rate: 1 mL/min
Temperature: 40 °C
Injection volume: 30 µL
Sample load: 0.4% (w/v)
Sample: Polyurethane
-2.00
0.00
2.00
4.00
6.00
8.00
10.00
12.00
14.00
16.00
2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00 4.20 4.40 4.60 4.80 5.00 5.20 5.40 5.60 5.80 6.00
µRIU
Minutes
© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64646
9
[ APPLICATION highlight ]
Analysis of Poly(methylmethacrylate co hydroxyethylmethacrylate) in THF using ACQUITY APC Columns
LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)
Eluent: THF
Detection: ACQUITY RI
Column: ACQUITY APC™ XT 4.6 x 150 mm x 3 (450Å, 125Å and 45Å in series), part numbers 186007010, 186007000 and 186006995, respectively
Flow rate: 1 mL/min
Temperature: 40 °C
Injection volume: 30 µL
Sample load: 0.4% (w/v)
Sample: Poly(methylmethacrylate co hydroxyethylmethacrylate)
µRIU
Minutes
-2.00
0.00
2.00
4.00
6.00
8.00
10.00
12.00
14.00
16.00
18.00
20.00
22.00
24.00
2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00 4.20 4.40 4.60 4.80 5.00 5.20 5.40 5.60 5.80 6.00
© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64647
10
[ APPLICATION highlight ]
Analysis of an Epoxy Resin in THF using ACQUITY APC Columns
LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)
Eluent: THF
Detection: ACQUITY RI
Column: ACQUITY APC™ XT 4.6 x 150 mm x 2 (125Å and 45Å in series), part numbers 186007000 and 186006995, respectively
Temperature: 40 °C
Injection volume: 20 µL
Sample load: 0.5% (w/v)
Sample: Epoxy resin
µRIU
Minutes
-1.00 -0.50 0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00
0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00
© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64648
11
[ APPLICATION highlight ]
Analysis of Poly(vinylpyrrolidone co methyl methacrylate) in THF using ACQUITY APC Columns
LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)
Eluent: THF
Detection: ACQUITY RI
Column: ACQUITY APC™ XT 4.6 x 150 mm x 2 (125Å and 45Å in series), part numbers 186007000 and 186006995, respectively
Temperature: 40 °C
Injection volume: 20 µL
Sample load: 0.5% (w/v)
Sample: Poly(vinylpyrrolidone co methyl methacrylate)
µRIU
Minutes
-2.00
-1.00 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00
10.00
0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00
© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64649
12
[ APPLICATION highlight ]
Analysis of Poly(methyl methacrylate) in THF using ACQUITY APC Columns
LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)
Eluent: THF
Detection: ACQUITY RI
Column: ACQUITY APC™ XT 4.6 x 150 mm x 2 (450Å and 125Å in series), part numbers 186007010 and 186007000, respectively
Temperature: 40 °C
Injection volume: 20 µL
Sample load: 0.2% (w/v)
Sample: Poly(methyl methacrylate)
µRIU
Minutes
-16.00
-14.00 -12.00 -10.00
-8.00 -6.00 -4.00 -2.00 0.00 2.00 4.00 6.00 8.00
10.00 12.00 14.00 16.00 18.00 20.00
0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00
© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64650
13
[ APPLICATION highlight ]
Analysis of poly(phenylglycidyl ether) co formaldehyde in THF using ACQUITY APC Columns
LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)
Eluent: THF
Detection: ACQUITY RI
Column: ACQUITY APC™ XT 4.6 x 150 mm x 2 (45Å and 45Å in series), part number 186006995
Temperature: 40 °C
Injection volume: 20 µL
Sample load: 0.2% (w/v)
Sample: Poly(phenylglycidyl ether) co formaldehyde
µRIU
Minutes
-40.00
-30.00 -20.00 -10.00
0.00 10.00 20.00 30.00 40.00 50.00 60.00 70.00
0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40
© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64652
14
[ APPLICATION highlight ]
Analysis of Poly(o-cresyl glycidyl ether) co formaldehyde in THF using ACQUITY APC Columns
LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)
Eluent: THF
Detection: ACQUITY RI
Column: ACQUITY APC™ XT 4.6 x 150 mm x 2 (45Å and 45Å in series), part number 186006995
Temperature: 40 °C
Injection volume: 20 µL
Sample load: 0.2% (w/v)
Sample: Poly(o-cresyl glycidyl ether) co formaldehyde
µRIU
Minutes
-40.00 -30.00 -20.00 -10.00
0.00 10.00 20.00 30.00 40.00
0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40
© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64653
15
[ APPLICATION highlight ]
Analysis of Poly(phenyl isocyanate) co formaldehyde in THF using ACQUITY APC Columns
LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)
Eluent: THF
Detection: ACQUITY RI
Column: ACQUITY APC™ XT 4.6 x 150 mm x 2 (45Å and 45Å in series), part number 186006995
Temperature: 40 °C
Injection volume: 20 µL
Sample load: 0.2% (w/v)
Sample: Poly(phenyl isocyanate) co formaldehyde
µRIU
Minutes
0.00 20.00 40.00 60.00 80.00
100.00 120.00 140.00 160.00 180.00 200.00 220.00
0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40
© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64654
16
[ APPLICATION highlight ]
Analysis of Poly(styrene), Low Molecular Weight in THF using ACQUITY APC Columns
LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)
Eluent: THF
Detection: ACQUITY RI
Column: ACQUITY APC™ XT 4.6 x 150 mm x 2 (45Å and 45Å in series), part number 186006995
Temperature: 40 °C
Injection volume: 20 µL
Sample load: 0.2% (w/v)
Sample: Poly(styrene), low molecular weight
µRIU
Minutes
-20.00 -15.00 -10.00
-5.00 0.00 5.00
10.00 15.00 20.00 25.00 30.00 35.00 40.00 45.00 50.00
0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40
© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64655
17
[ APPLICATION highlight ]
Analysis of Poly(isobutyl methacrylate) in THF using ACQUITY APC Columns
LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)
Eluent: THF
Detection: ACQUITY RI
Column: ACQUITY APC™ XT 4.6 x 150 mm x 2 (450Å and 125Å in series), part numbers 186007010 and 186007000, respectively
Temperature: 40 °C
Injection volume: 20 µL
Sample load: 0.2% (w/v)
Sample: Poly(isobutyl methacrylate)
µRIU
Minutes
-6.00
-4.00
-2.00
0.00
2.00
4.00
6.00
8.00
0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00
© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64656
18
[ APPLICATION highlight ]
Analysis of Poly(bisphenol A co epichlorohydrin) in DMF using ACQUITY APC Columns
LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)
Eluent: DMF with 10 mM LiCl
Detection: ACQUITY RI
Column: ACQUITY APC™ XT 4.6 x 150 mm x 2 (450Å and 125Å in series), part numbers 186007010 and 186007000, respectively
Temperature: 40 °C
Injection volume: 20 µL
Sample load: 0.2% (w/v)
Sample: Poly(bisphenol A co epichlorohydrin)
µRIU
Minutes
-1.00 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00
10.00
0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60
© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64657
19
[ APPLICATION highlight ]
Analysis of Poly(vinylacetate) in DMF using ACQUITY APC Columns
LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)
Eluent: DMF with 10 mM LiCl
Detection: ACQUITY RI
Column: ACQUITY APC™ XT 4.6 x 150 mm x 2 (450Å and 125Å in series), part numbers 186007010 and 186007000, respectively
Temperature: 40 °C
Injection volume: 20 µL
Sample load: 0.2% (w/v)
Sample: Poly(vinylacetate)
µRIU
Minutes
-1.00
-0.50
0.00
0.50
1.00
1.50
2.00
2.50
3.00
3.50
1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80
© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64658
20
[ APPLICATION highlight ]
Analysis of Poly(9,9 di-n-octylfluorenyl 2,7-diyl) in Toluene using ACQUITY APC Columns
LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)
Eluent: Toluene
Detection: ACQUITY RI
Column: ACQUITY APC™ XT 4.6 x 150 mm x 2 (450Å and 125Å in series), part numbers 186007010 and 186007000, respectively
Temperature: 40 °C
Injection volume: 20 µL
Sample load: 0.5% (w/v)
Sample: Poly(9,9 di-n-octylfluorenyl 2,7-diyl)
µRIU
Minutes
0.00
0.50
1.00
1.50
2.00
2.50
3.00
3.50
4.00
4.50
0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00 4.20
© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64659
21
[ APPLICATION highlight ]
Analysis of Poly(styrene) Standards in THF using ACQUITY APC Columns
LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)
Eluent: THF
Detection: ACQUITY RI
Column: ACQUITY APC™ XT 4.6 x 150 mm x 2 (125Å and 45Å in series), part numbers 186007000 and 186006995, respectively
Temperature: 40 °C
Injection volume: 10 µL
Sample load: 1 mg/mL
Sample: Poly(styrene) standards
µRIU
Minutes
0.00
5.00
10.00
15.00
20.00
25.00
30.00
35.00
40.00
45.00
50.00
1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20
2800
6570
9890
18.2
K
35K
© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64660
22
[ APPLICATION highlight ]
Analysis of Poly(dimethyl silicone) in THF using ACQUITY APC Columns
LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)
Eluent: THF
Detection: ACQUITY RI
Column: ACQUITY APC™ XT 4.6 x 150 mm x 4 (125Å and 3 x 45Å in series), part numbers 186007000 and 186006995, respectively
Temperature: 40 °C
Injection volume: 10 µL
Flow rate: 0.6 mL/min
Sample load: 0.2% (w/v)
Sample: Poly(dimethyl silicone)
© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64711
µRIU
0.00
0.30
0.60
0.90
1.20
Minutes5.60 7.00 8.40 9.80 11.20
23
[ APPLICATION highlight ]
Analysis of Poly(dimethyl siloxane) in Toluene using ACQUITY APC Columns
LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)
Eluent: Toluene
Detection: ACQUITY RI
Column: ACQUITY APC™ XT 4.6 x 150 mm x 3 (125Å and 2 x 45Å in series), part numbers 186007000 and 186006995, respectively
Temperature: 60 °C
Injection volume: 10 µL
Flow rate: 1 mL/min
Sample load: 0.2% (w/v)
Sample: Poly(dimethyl siloxane)
© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64712
µRIU
0.00
2.80
5.60
8.40
11.20
Minutes
2.60 3.90 5.20 6.50 7.80
24
[ APPLICATION highlight ]
Aqueous Analysis of Pullulans using ACQUITY APC Columns
LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)
Eluent: 100 mM sodium nitrate in 80:20 water/acetonitrile
Detection: ACQUITY RI
Column: ACQUITY APC™ AQ 4.6 x 150 mm, 200Å, part number 186006985
Temperature: 40 °C
Injection volume: 20 µL
Sample load: 1 mg/mL
Sample: Pullulan standards
-2.00
0.00 2.00 4.00 6.00 8.00
10.00 12.00 14.00 16.00 18.00 20.00
1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20
107K
47K
21K
9600
6100
µRIU
Minutes
© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64661
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©2016 Waters Corporation. Produced in the U.S.A.October 2016 720004649EN TC-PDF