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Advanced Cell Culture Product Selection Guide

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    Prepare. Grow. Analyze.Advanced cell culturesystems or every challenge.

    EMD Millipore is a division o Merck KGaA, Darmstadt, Germany

    http://www.millipore.com/?cid=BIOS-S-EPDF-1089-1207-DS
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    P R E P A R E

    G R O W

    A N A L Y Z E

    With industry-leading, innovative designs or ltration devices and over

    50 years o membrane technology expertise, EMD Millipore oers a wide

    range o solutions to make the repetitive yet critical steps o preparing

    cells and media accurate, quick and easy.

    Find the perect culture conditions or all your cell culture needs with EMD

    Millipores wide variety o high quality devices and suraces. Not only do our

    products provide the most convenient, reliable, analysis-ready cell culture,

    but they also yield cell growth, structure, and unction that more closely mimic

    what occurs in vivo. Spend less time growing cells and umbling with clumsy

    devices and more time on your research.

    For multidimensional analysis o cellular phenotypes with respect to time,

    space and changing conditions, we provide reagents, platorms and devices thatsuccessully meld sophisticated technology with ease o use and optimization.

    Hundreds o cell culture products. Find everything you need atwww.millipore.com/cellculture

    http://www.millipore.com/catalogue/module/c78139?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/module/c78139?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/cellbiology/flx4/cell_culture?cid=BIOS-S-EPDF-1089-1207-DS
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    Table o Contents

    PREPARE

    Reagents 06 Water or Cell Culture 06

    OmniPur Grade Research Reagents 07

    OmniPur WFI-Quality Water, sterile, cell culture tested 07

    Molecular Biology Grade Reagents 08

    Transection Reagents 08

    Tools 09

    Fast-Trap Virus Purication and Concentration Kits 09

    Sterile Filtration 10

    Stericup and Steritop Filters 10Sterifip Filters 10

    Millex Syringe Filters 11

    Sterivex Filter Units 11

    GROW

    Cells and Media 14

    Primary Endothelial, Epithelial and FibroblastCells and Media 14

    Custom-Formulated Liquid Cell Culture Media 15

    Cultureware 16

    Millicell Hanging and Standing Inserts 16Millicell and MultiScreen Multiwell Plates 17

    Millicell

    EZslides

    18Millicell ERS-2 Voltohmmeter 18

    Millicell HY Multilayer Flasks 19

    ECMs and Coated Cultureware 19

    ANALYZE

    Reagents 22

    Antibodies 22

    Cell-based Assays 22

    Assay Tools 23

    MultiScreen Plates and 8-well Strips or Elispot Assays 23

    Millicell -Angiogenesis Assay Kit 23

    Millicell -Migration Assay Kit 24AXIS Axon Isolation Device 24

    Millicell and MultiScreen Multiwell Plates 25

    Diusion Chamber Kits & Accessories 25

    Cell Analysis Platorms 26

    Scepter 2.0 Cell Counter 26

    Muse Cell Analyzer 26

    guava easyCyte Flow Cytometry 27

    Amnis FlowSight System 27

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    P R E P A R E

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    Prepare or success.

    Use our reagents or molecular biology and cell

    culture, together with our trusted sterile ltration

    devices, to minimize contamination risk andmaximize workfow eciency while preparing

    your cells and media.

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    Reagents

    Water or Cell CultureEMD Millipore certied water or cell culture is ideal or

    research use by scientists perorming critical cell culture

    experiments in labs that use under 50 L per year o

    ultrapure water. Water or cell culture is sterile,

    cell-culture tested and ree o pyrogens, mycoplasma,

    calcium and magnesium.

    KEY BENEFITS

    Certied water quality: Each bottle is delivered with aCerticate o Quality. Strict quality control tests andproduction in an ISO 9001/cGMP environment providehigh-quality water or critical cell culture experiments.

    Mycoplasma-ree water.

    Trusted manuacturer: Manuactured by EMD Millipore,leader in water purication and cell culture productsand techniques.

    Easy traceability: one label can be peeled o and axedto a laboratory notebook or traceability in experiments.

    Volume range adapted to user needs: available in 1 L,0.5 L and 125 mL bottles in dierent package sizes.

    Description Catalogue No.

    1 L Ultrapure Water or Cell Culture, 1/pk H20CC1001

    1 L Ultrapure Water or Cell Culture, 6/pk H20CC1006

    500 mL Ultrapure Water or Cell Culture, 1/pk H20CC0501

    500 mL Ultrapure Water or Cell Culture, 6/pk H20CC0506

    125 mL Ultrapure Water or Cell Culture, 6/pk H20CC0106

    125 mL Ultrapure Water or Cell Culture, 24/pk H20CC0124

    http://www.millipore.com/catalogue/item/H20CC1001?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/H20CC1006?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/H20CC0501?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/CC050?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/H20CC0501?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/CC050?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/H20CC0106?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/H20CC0124?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/H20CC1001?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/CC050?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/CC050?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/H20CC0124?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/H20CC0106?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/H20CC0506?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/H20CC0501?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/H20CC1006?cid=BIOS-S-EPDF-1089-1207-DS
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    Reagents

    OmniPur WFI-QualityWater, Sterile,Cell Culture TestedOur Calbiochem brand Water For Injection (WFI)

    -quality water or cell culture is tested ollowing

    the most recent United States Pharmacopeia (USP)

    WFI guidelines. WFI-quality water can be used or

    a wide range o cell culture applications, including

    the preparation o media and buers, dilution andreconstitution o lyophilized reagents. Sizes range rom

    500 mL to 200 L, meeting your needs at every scale.

    Description Size Catalogue No.

    WFI-Quality Water, sterile, cell

    culture tested

    500 mL 4.86505.0500

    1 L 4.86505.1000

    5 L 4.86505.5000

    10 L 4.86505.9010

    20 L 4.86505.9020

    200 L 4.86505.9200

    OmniPur Grade

    Research ReagentsExperience peace o mind when you use OmniPur

    grade antibiotics, buers, detergents, salts, and

    molecular markers or your cell and tissue culture,

    transection and selection, molecular biology

    manipulations and cell analysis.

    Description Size Catalogue No.

    OmniPur Agarose PCR Pluse 25 gm 2005-25GM

    100 gm 2010-100GM

    500 gm 2020-500GM

    OmniPur 10X PBS Liquid

    Concentrate

    4 L 6505-4L

    20 L 6504-20L

    OmniPur Sodium Dodecyl Sulate 500 gm 7910-500GM

    5 kg 7960-5KGKEY BENEFITS

    Well-published: OmniPur reagents have been success-ully used and cited in many peer-reviewed publications.

    DNase, RNase, protease-tested: OmniPur reagentsare DNase-, RNase-, and protease-tested to ensurethe integrity o your starting material, improvingdownstream results.

    Package sizes or every scale: With multiple sizechoices ranging rom 100 g to 50 kg, OmniPurreagents provide a seamless transition rom researchbench to production.

    For more inormation and to view the entire OmniPur product listing, visit www.emdmillipore.com/OmniPur

    http://www.millipore.com/catalogue/item/4.86505.0500?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/4.86505.1000?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/4.86505.5000?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/4.86505.9010?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/4.86505.9020?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/4.86505.9200?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/2005-25GM?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/2010-100GM?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/2020-500GM?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/6505-4L?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/6504-20L?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/7910-500GM?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/7960-5KG?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/7960-5KG?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/4.86505.9200?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/4.86505.9020?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/4.86505.9010?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/4.86505.5000?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/4.86505.1000?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/4.86505.0500?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/7910-500GM?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/6504-20L?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/6505-4L?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/2020-500GM?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/2010-100GM?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/2005-25GM?cid=BIOS-S-EPDF-1089-1207-DS
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    Reagents

    Molecular BiologyGrade ReagentsPerorm worry-ree cell culture and genetic

    manipulations o your cells using EMD Millipores

    Calbiochem brand antibiotics, buers and detergents,

    which are manuactured in a controlled environment

    and perorm with exceptional consistency to ensure

    the best results, lot ater lot. Popular Calbiochem

    antibiotics include G418 Sulate and Hygromycin B.

    Our wide selection o buers eatures ormulations

    tailored or specic applications. For greater fexibility,

    you can choose some buers as either solid powders

    or as ready-to-use solutions. Calbiochem has been

    a brand synonymous with high quality detergents or

    over 50 years, and our portolio includes Ionic, Non-

    ionic and Zwitterionic detergents and Non-detergent

    Sulobetaines (NDSBs).

    Description Catalogue No.

    G418 Sulate, Cell Culture Tested 345810

    Hygromycin B, Streptomyces sp. 400051

    G418 Sulate, Sterile-Filtered Aqueous Solution,

    Cell Culture Tested

    345812

    Blasticidin S, HCl, Streptomyces griseochromogenes 203350Hygromycin B. Sterile-Filtered in PBS, Cell Culture Tested 400052

    Amphotericin B, Streptomyces sp. 171375

    Actinomycin D, 7-Amino- 129935

    Puromycin, DiHCl. Cell Culture Tested 540411

    Bleomycin Sulate, Streptomyces verticillus 203401

    Doxorubicin, HCl 324380

    For complete product listings and comprehensive guides to antibiotic selection,

    please visit our online antibiotics resource atwww.emdmillipore.com/antibiotics

    Transection ReagentsTransection is a science and an art. The right

    transection reagent is the one that maximizes

    transection eciency, minimizes cytotoxicity, and results

    in the desired level o gene expression or silencing. EMD

    Millipore recognizes that there is not a one-size-ts-all

    solution to your transection needs. We provide the

    breadth o transection reagents necessary to give you

    the reedom to design the perect experiment.

    KEY BENEFITS

    Superior alternatives to other DNA transer techniquesincluding calcium phosphate coprecipitation, electropo-ration, microinjection, biolistic particle delivery, andcomplex ormation with DEAE-dextran.

    Save time with simplied, optimized protocols, includingno media changes or most reagents and cell types.

    Well-published: Visit the transection resource website,www.emdbiosciences.com/transection, or transection

    conditions described in a growing list o publicationsciting our transection reagents.

    KEY BENEFITS Cell Culture Tested.

    Superior potency.

    More than 10,000 citations since year 2000.

    Description Catalogue No.

    GeneJuice reagent: high eciency or a

    broad range o cells

    70967

    NanoJuice kit: optimum eciency or

    dicult-to-transect cells

    71902

    293-Free reagent: protein production in

    suspension Hek293 cells

    72181

    NovaCHOice kit: protein production in

    suspension CHO cells

    72622

    Insect GeneJuice reagent: insect cell

    transection

    71259

    RiboJuice reagent: siRNA transection 71281

    ProteoJuice reagent: protein or peptide

    transection

    71115

    http://www.millipore.com/catalogue/item/345810?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/400051?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/345812?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/203350?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/400052?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/171375?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/129935?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/540411?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/203401?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/324380?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/?cid=BIOS-S-EPDF-1089-1207-DShttp://www.emdbiosciences/transfectionhttp://www.emdbiosciences/transfectionhttp://www.millipore.com/catalogue/item/70967?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/71902?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/72181?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/72622?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/71259?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/71281?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/71115?cid=BIOS-S-EPDF-1089-1207-DShttp://www.emdbiosciences/transfectionhttp://www.millipore.com/?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/71115?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/71281?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/71259?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/72622?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/72181?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/71902?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/70967?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/324380?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/203401?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/540411?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/129935?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/171375?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/400052?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/203350?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/345812?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/400051?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/345810?cid=BIOS-S-EPDF-1089-1207-DS
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    Tools

    For more inormation, visit www.millipore.com/catalogue/module/c78139

    Fast-Trap Virus

    Purication andConcentration KitsBuilding on our ltration technology, weve created

    a unique method or puriying and concentrating

    adenovirus and lentivirus. Our kits provide all the

    necessary components, including Sterifip lters, or

    ast, sae and easy virus purication in as little as 2 hours

    with outstanding yield.

    KEY BENEFITS Saves time: protocol can be completed in as little as

    two hours.

    Higher yield: recover up to 70% o viral particles.

    Dependable: simple protocol ensures quality results.

    Easy: Sterifip lters eliminate messy purication stepsand expensive equipment.

    Sae: Closed vacuum-driven device eliminatespotential spills.

    Description Catalogue No.

    Fast-Trap Adenovirus Purication and

    Concentration Kit

    FTAV00003

    Fast-Trap Lentivirus Purication and

    Concentration Kit

    FTLV00003

    http://www.millipore.com/catalogue/module/c78139?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/FTAV00003?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/FTLV00003?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/module/c78139?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/module/c78139?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/FTLV00003?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/FTAV00003?cid=BIOS-S-EPDF-1089-1207-DS
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    0

    Sterifip FiltersThe unique design o Sterifip ltration units reduces

    risk o contamination by eliminating one liquid

    handling step. Connect the Sterifip lter to any

    standard 50 mL tube containing sample, fip it over,

    and apply vacuum.

    Description Catalogue No.

    Sterifip-GP 0.22 PES Filter, 50 mL SCGP00525

    Sterifip-HV 0.45 Durapore (PVDF) Filter,

    50 mL

    SE1M003M00

    KEY BENEFITS

    Filters directly into a 50 mL conical tube to urtherminimize liquid transer.

    Nylon net membrane available or cell separation.

    Other membrane options include Millipore Express(PES) membrane or ast fow and Durapore PVDFmembrane or low protein binding.

    Stericup andSteritop FiltersStericup and Steritop lters are the most trusted

    devices or sterile ltration o cell culture media, buers

    and reagents. Stericup lters combine a lter unit witha receiver fask and cap or processing and storage and

    provide superior recovery. Steritop bottle top lters can

    be used with bottles having a 33 mm or 45 mm opening.

    Description Catalogue No.

    Stericup-GP 0.22 PES Filter, 500 mL SCGPU05RE

    Stericup-GP 0.22 PES Filter, 1000 mL SCGPU11RE

    Stericup-GV 0.22 Durapore Filter, 500 mL SCGVU05RE

    Stericup-GV 0.22 Durapore Filter, 1000 mL SCGVU11RE

    KEY BENEFITS

    Both lters available with ast fow Millipore ExpressPLUS and low-protein-binding Durapore membranes.

    No loss o media in the ltration process.

    0.1 m pore size available or mycoplasma removal.

    Eliminating contaminants rom your cell growth media and additives is absolutelycrucial to preserving the integrity and accuracy o your cell cultures. EMD Milliporescomprehensive line o sterile ltration tools have been specically designed to

    address these needs and to ensure the reproducibility o your downstream analyses.To learn more, please visit: www.millipore.com/cellculture

    Sterile Filtration

    http://www.millipore.com/catalogue/item/SCGP00525?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SE1M003M00?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SCGPU05RE?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SCGPU11RE?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SCGVU05RE?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SCGVU11RE?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/module/c78139?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/module/c78139?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/cellbiology/flx4/cell_culture?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SE1M003M00?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SCGP00525?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SCGVU11RE?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SCGVU05RE?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SCGPU11RE?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SCGPU05RE?cid=BIOS-S-EPDF-1089-1207-DS
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    Sterivex Filter UnitsSterivex lter units work with syringes, peristaltic

    pumps or pressure vessels, eliminating the risk o

    contamination associated with decanting or pipetting.

    Description Catalogue No.

    2000 mL Sterivex-GP 0.22 m Millipore

    Express PLUS (PES) Filter Unit, 10/pk

    SVGPB1010

    1000 mL Sterivex-GV 0.22 m Durapore (PVDF)

    Filter Unit, 10/pk

    SVGVB1010

    1000 mL Sterivex-HV 0.45 m Durapore (PVDF)

    Filter Unit, 10/pk

    SVHVB1010

    KEY BENEFITS

    Ideal or volumes 100 mL to 2 L.

    Filter directly into virtually any storage container.

    Available with a selection o high perormancemembranes.

    Sterile Filtration

    1

    Millex

    Syringe FiltersSterile Millex syringe lters provide convenient

    sterilization o small volumes and are ideal or solutions

    such as antibiotics and tissue culture additives. The

    lters unsurpassed quality and consistency o results

    has led to the development o many sample preparation

    methods that speciy Millex lters.

    Description Catalogue

    No.

    33 mm Millex-GP 0.22 PES Syringe Filter, 50/pk SLGP033RS

    33 mm Millex-HV 0.45 Durapore Syringe Filter, 50/pk SLHV033RS

    25 mm Vented Millex-GS 0.22 MCE Syringe Filter, 50/pk SLGSV255F

    KEY BENEFITS

    Filter diameters to match your sample volume:4, 13, 25, 33, and 50 mm.

    Minimal hold-up volume or less sample loss.

    High perormance membranes or ast fow and lowprotein binding.

    Various inlet/outlet connection combinations providefexibility in usage.

    http://www.millipore.com/catalogue/item/SVGPB1010?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SVGVB1010?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SVHVB1010?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SLGP033RS?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SLHV033RS?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SLGSV255F?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SVHVB1010?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SVGVB1010?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SVGPB1010?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SLGSV255F?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SLHV033RS?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SLGP033RS?cid=BIOS-S-EPDF-1089-1207-DS
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    2

    G R O W

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    1

    Cells, media andcultureware to helpyour research grow.

    Whatever your cell culture growth needs,

    we have a plate, insert or vessel that can help

    you obtain the optimal results. Our specialty cells,

    media and medium supplements include epithelial,

    endothelial, neural and stem cells and theiroptimized media, as well as extracellular matrix

    coatings, cytokines and growth actors.

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    4

    Primary Endothelial,

    Epithelial and FibroblastCells and MediaEndoGRO Human Umbilical Vein Endothelial Cells

    (HUVEC) and specially ormulated media provide a

    valuable, easy-to-culture cell model or cancer and

    vascular biology research.

    EpiGRO primary epithelial cells and specially ormulated

    media improve epithelial cell culture or epidermal

    keratinocytes, small airway epithelial cells, corneal cells,

    and prostate epithelial cells.

    FibroGRO primary human oreskin broblasts and

    specially ormulated media improve broblast cell

    culture and are derived rom normal human oreskin

    and have been isolated and manuactured under

    xeno-ree conditions.

    KEY BENEFITS

    Serum-ree or low-serum ormulations.

    Improved viability and morphology.

    Increased cell culture longevity.

    Faster prolieration rates.

    No phenol red or other additives that producemasking eects.

    Unique light blocking and temperature-monitoredpackaging to promote media stability andbetter results.

    Extensive QC to ensure lot-to-lot consistency.

    EMD Millipore oers an extensive range o embryonic, neural, mesenchymal and primary cells or

    both human and rodent studies. Optimized media are available in both serum-ree and/or eeder-ree

    ormulations, validated specically or the specic cell type o interest. Supporting the ull rangeo cell culture products are ECM proteins, recombinant growth actors, eeder cells, supplements,

    passaging and cryopreservation reagents, and a complete range o reprogramming tools or developing

    and studying induced pluripotent stem (iPS) cells.

    Explore our entire stem cell product oering by visiting www.millipore.com/stemcells

    Description Catalogue No.

    EpiGRO Human Epithelial Cells and Complete Media Kits

    Human Epidermal Keratinocytes, Neonatal SCCE020

    Human Corneal Epithelial Cells SCCE016

    Human Prostate Epithelial Cells, Adult SCCE019Human Small Airway Epithelial Cells SCCE009

    Visit www.millipore.com/epigro or details.

    EndoGRO Endothelial Cells and Complete Media Kits

    EndoGRO Human Umbilical Vein Endothelial

    Cells (HUVEC)

    SCCE001

    Visit www.millipore.com/endogro or details.

    Selected FibroGRO Fibroblast Cells and Complete Media Kits

    FibroGRO Xeno-Free Human Foreskin

    Fibroblasts

    SCC058

    FibroGRO Xeno-Free Human FibroblastExpansion Medium SCM037

    Cells and Media

    http://www.millipore.com/stemcell/stm4/stemcellhome?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SCCE020?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SCCE016?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SCCE019?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SCCE009?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SCCE001?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SCC058?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SCM037?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/stemcell/stm4/stemcellhome?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/stemcell/stm4/stemcellhome?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SCM037?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SCC058?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SCCE001?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SCCE009?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SCCE019?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SCCE016?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SCCE020?cid=BIOS-S-EPDF-1089-1207-DS
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    Turnaround Time Custom media delivered 30 business days

    ater purchase order is received

    IncludedServices

    pH testing

    Osmolarity testing

    Sterility testing

    Order volume 10 L minimum

    We can accommodate large-scaleproduction needs

    Whether you need a published ormulation,

    customer-developed ormulation, modied standard

    medium ormulation or one that includes non-

    standard components like growth actors, we have

    the experience and know-how to ulll your specic

    needs. Our scientists will work with you to nd or

    design the custom ormulation that perorms to

    your specications.

    Order media services at www.millipore.com/reply/orm/custom_media

    Cells and Media

    1

    Custom-Formulated Liquid Cell Culture Media

    http://www.millipore.com/reply/form/custom_media?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/reply/form/custom_media?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/reply/form/custom_media?cid=BIOS-S-EPDF-1089-1207-DS
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    6

    Millicell Hanging andStanding InsertsMillicell membrane-based cultureware promotes the

    natural growth o adherent and suspension cell lines.

    The optimized membranes result in cells with structure

    and unction that more closely mimic their in vivo

    counterparts, leading to more biologically relevant

    results. Whether you are co-culturing or conducting

    permeability assays or looking or high cell viability or

    as long as 40 days, we have a large selection o inserts in

    dierent sizes and membrane typesincluding our best-

    in-class organotypic inserts.

    KEY BENEFITS

    Optimized membranes or reliable monolayer ormation.

    Transparent membranes or easy cell growth monitoring.

    Extensive choice o device platorms available.

    Description Catalogue No.

    Hanging Inserts

    Millicell Hanging Cell Culture Insert,polyethylene terephthalate 8.0 m PIEP12R48

    Millicell Hanging Cell Culture Insert,

    polyethylene terephthalate, 1.0 m

    PIRP12R48

    Standing Inserts

    Millicell Cell Culture Organotypic Insert,

    30 mm, hydrophilic PTFE, 0.4 m

    PICM0RG50

    Millicell Cell Culture Insert, 30 mm,

    hydrophilic PTFE, 0.4 m

    PICM03050

    Millicell Cell Culture Insert, 12 mm

    hydrophilic PTFE, 0.4 m

    PICM01250

    Tissue-Culture Treated Plates

    Millicell 6-Well Cell Culture Receiver Plate,

    TC, Sterile

    PIMWS0650

    Millicell 12-Well Cell Culture Receiver

    Plate, TC, Sterile

    PIMWS1250

    Millicell 24-Well Cell Culture Receiver

    Plate, TC, Sterile

    PIMWS0650

    For the entire cell culture insert catalogue, please visit www.millipore.com/cellculture

    Cultureware

    http://www.millipore.com/catalogue/item/PIEP12R48?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PIRP12R48?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PICM0RG50?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PICM03050?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PICM01250?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PIMWS0650?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PIMWS1250?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PIMWS0650?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/module/c78139?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/module/c78139?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/cellbiology/flx4/cell_culture?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PIMWS0650?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PIMWS0650?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PIMWS1250?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PIMWS0650?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PIMWS0650?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PICM01250?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PICM03050?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PICM0RG50?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PIRP12R48?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PIEP12R48?cid=BIOS-S-EPDF-1089-1207-DS
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    Cultureware

    1

    Millicell andMultiScreenMultiwell PlatesOur membrane-based and plastic multiwell plates come

    in a ull range o well, membrane, and pore sizes. Ourspecially designed Millicell plateswith their apical

    assists, basolateral access, and elevated eetare ideal

    or Caco-2 cell cultures. Millicell plates are optimized to

    sustain high integrity cell monolayers. Our MultiScreen

    plates are used or a variety o applications including

    Elispot and migration/invasion/chemotaxis assays. I

    there is a conguration we dont oer, dont hesitate to

    ask us about special congurations and custom orders.

    KEY BENEFITS Simpliy handling o multiple samples simultaneously.

    Maintain assay integrity and prevent monolayerdisruption, contamination or damage.

    Assemblies include a choice o a multi-well orsingle-well eeder tray.

    Description Catalogue No.

    Millicell Plates Millicell-96 Cell Culture Insert Plate, polycarbonate, 0.4 m,

    96-well receiver tray

    PSHT004S5

    Millicell-96 Cell Culture Insert Plate,polycarbonate, 0.4 m,

    single-well receiver tray

    PSHT004R5

    Millicell-24 Cell Culture Insert Plate, polyethylene terephtha-

    late, 1.0 m

    PSRP010R5

    Millicell-24 Cell Culture Insert Plate, polycarbonate, 0.4 m PSHT010R5

    MultiScreen Plates

    MultiScreenhts IP, 0.45 m, 8-Well Strips, clear, sterile M8IPS4510

    MultiScreenhts IP, 0.45 m, clear, sterile MSIPS4510

    MultiScreen-HV, 0.45 m, clear, non-sterile MAHVN4550

    MultiScreenhts BV, 1.2 m, clear, non-sterile MSBVN1250

    For more plate congurations, please visit www.millipore.com/cellculture

    http://www.millipore.com/catalogue/item/PSHT004S5?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PSHT004R5?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PSRP010R5?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PSHT010R5?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/M8IPS4510?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MSIPS4510?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MAHVN4550?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MSBVN1250?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/module/c78139?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/module/c78139?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/cellbiology/flx4/cell_culture?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MSBVN1250?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MAHVN4550?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MSIPS4510?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/M8IPS4510?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PSHT010R5?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PSRP010R5?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PSHT004R5?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PSHT004S5?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/M8IPS4510?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MSBVN1250?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MAHVN4550?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MSIPS4510?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/M8IPS4510?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PSHT010R5?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PSRP010R5?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PSHT004R5?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PSHT004S5?cid=BIOS-S-EPDF-1089-1207-DS
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    Cultureware

    8

    Millicell EZ SLIDESSimpliy your analysis by culturing, xing, staining, and

    viewing your samples all in one chamber slide device.

    With our breakaway tabs, you can easily remove wells

    without worrying about breaking slides or harming cells.

    Our easy-to-use Millicell EZ slides come in 4-well and

    8-well glass slide ormats.

    MillicellERS-2 VoltohmmeterThe Millicell-ERS (Electrical Resistance System) reliably

    measures membrane potential and resistance o

    epithelial cells in culture [or the Trans Epithelial Electrical

    Resistance (TEER)]. This device uses alternating current

    (AC) to qualitatively measure cell monolayer health and

    quantitatively measure cell confuence.

    KEY BENEFITS

    Breakaway tabs allow wells to be detached easily romthe slides without glue or gasket residue; no toolsare required.

    Polypropylene wells are resistant to most xing andstaining reagents used, so you can grow and staincells in the well, eliminating the need to removechambers prior to analysis.

    Units are stackable.

    Easy to visualize cells and acquire data throughtransparent lid.

    KEY BENEFITS(o alternating current compared to traditional

    direct current measurements)

    Membrane voltage and voltage electrode oset donot aect resistance measurements.

    The zero net charge on the cells eliminates the adverseeects o DC current on the cell membrane.

    There is no electrochemical deposition oelectrode metals.

    Description Catalogue No.

    Millicell EZ slide (4-well, glass, 16/pk) PEZGS0416

    Millicell EZ slide (4-well, glass, 16/pk) PEZGS0496

    Millicell EZ slide (8-well, glass, 16/pk) PEZGS0816

    Millicell EZ slide (8-well, glass, 16/pk) PEZGS0896

    Millicell EZ slide Microscope Slide Holder PEZXMSH01

    Millicell EZ slide, unprinted, 4-well, glass

    (without pink ink), nonsterile, 16/pk

    PEZGUP0416

    Millicell EZ slide , with conocal cover slip,

    nonsterile (4-well, 16/pk)

    PEZC0N0416

    Description Catalogue No.

    Millicell ERS-2 Voltohmmeter MERS00002

    Replacement Electrodes, 1 pair MERSSTX01

    To learn more, please visit www.millipore.com/cellculture

    http://www.millipore.com/catalogue/item/PEZGS0416?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PEZGS0496?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PEZGS0816?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PEZGS0896?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PEZXMSH01?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PEZGUP0416?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PEZC0N0416?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MERS00002?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MERSSTX01?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/module/c78139?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/module/c78139?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/cellbiology/flx4/cell_culture?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MERSSTX01?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MERS00002?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PEZC0N0416?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PEZGUP0416?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PEZXMSH01?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PEZGS0896?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PEZGS0816?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PEZGS0496?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PEZGS0416?cid=BIOS-S-EPDF-1089-1207-DS
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    MillicellHY Multilayer FlasksThe Millicell HY fask provides consistent high cell yields,

    uniorm health across all layers, and a smaller incubator

    ootprint. Designed to acilitate high recovery o healthy

    cells, the fasks unique design is easy to use, intuitive,

    prevents spillover between layers or media leaking, and

    oers stackability.

    ECMs andCoated CulturewareChoose rom a wide variety o ECM proteins and coated

    cultureware to meet the individual needs o your cell

    line. Take advantage o the convenience o precoated

    cultureware or the fexibility o making your own

    coatings with our ECM proteins and plates. No matter

    what your research demands, we have a ormat to meet

    your needs.

    KEY BENEFITS

    Easy media removal rom fask with ergonomiccap location.

    Same volume o media per unit area required astraditional T fasks.

    Low prole o the fasks allows or a comortablet under microscopes or monitoring cell healthand confuency.

    Description Catalogue No.

    Millicell HY 3-layer cell culture fask,

    T-600, sterile

    PFHYS0616

    Millicell HY 5-layer cell culture fask,

    T-1000, sterile

    PFHYS1008

    Description Catalogue No.

    Human Extracellular Matrix Proteins

    Human Collagen Type I CC050

    Human Collagen Type II CC052

    Human Plasma Fibronectin Puried Protein FC010

    Human Laminin (pepsinized) Puried Protein AG56P

    Human Merosin CC085

    Vitronectin, Human Puried Protein CC080

    Cultureware

    1To explore our complete ECM product oering, visit www.millipore.com/ecm

    Description Catalogue No.

    Millicoat ECM Coated Tissue Culture Plates

    (24-well plates also available)

    6-well Plate with Collagen I Coating PICL06P05

    6-well Plate with Poly-D-Lysine Coating PIDL06P05

    6-well Plate with Fibronectin Coating PIFB06P05

    ECM Cell Culture Optimization Array

    ECM Cell Culture Optimization Array

    (Colorimetric, 96-well, 1 kit)

    ECM541

    http://www.millipore.com/catalogue/item/PFHYS0616?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PFHYS1008?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/CC050?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/CC052?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/FC010?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/AG56P?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/CC085?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/CC080?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/module/c77812?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PICL06P05?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PIDL06P05?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PIFB06P05?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/ECM541?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/ECM541?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/module/c77812?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/module/c77812?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PIFB06P05?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PIDL06P05?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PICL06P05?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/CC080?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/CC085?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/AG56P?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/FC010?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/CC052?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/CC050?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PFHYS1008?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PFHYS0616?cid=BIOS-S-EPDF-1089-1207-DS
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    0

    A N A L Y Z E

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    2

    Cell biology assaysand platormsor insightul

    cellular analysis.Visualize and quantiy cellular processes to connect

    morphology to behavior using our cell-based assay

    reagents, tools and our novel technologies or high

    content data collection and analysis.

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    Cell-based AssaysWe oer a signicant portolio o live cell, whole-cell

    and cell-based activity assays and reporter systems or

    direct and indirect detection. These technologies acilitate

    protein target validation, identiy cellular pathways and

    determine mechanism o action or lead optimization

    environments.

    Featured products include:

    More than 400 phosphospecic

    antibodies, including the best-selling4G10 phosphotyrosine antibody

    Large selection of antibodies to

    neuroscience markers

    Antibodies with epitope tags,

    including an exclusive anti-Myc tag

    clone 4A6 antibody

    Description Catalogue No.

    LentiBrite GFP-LC3 Lentiviral Biosensor 17-10193

    LentiBrite GFP-LC3 Control Mutant

    Lentiviral Biosensor

    17-10189

    LentiBrite RFP-LC3 Lentiviral Biosensor 17-10143

    LentiBrite RFP-LC3 Control Mutant

    Lentiviral Biosensor

    17-10188

    KEY BENEFITS

    All-in-one, quantitative and optimized transwell assaysprovide convenient and reliable quantication o cellmigration and invasion.

    Activity assays or key signaling proteins illuminate cellcycle, chromosomal organization, cell polarity, nucleardynamics, apoptosis, tumorigenicity, and more.

    New LentiBrite Lentiviral Biosensors are pre-packagedlentiviral particles encoding important and oundationalproteins o autophagy, apoptosis, and cell structure andenable visualization under dierent cell/disease states in

    live cell and in vitroanalysis.

    KEY BENEFITS

    Backed by our best-in-industry technical serviceteam dedicated to our antibodies customers.

    Most comprehensive portolio o antibodies and as-says across the lie sciences industry.

    100% guaranteed: I you are not completely satisedwith the perormance o EMD Millipore antibod-ies or small molecules, contact a technical supportspecialist or assistance or ull credit* against uturepurchases.

    2

    AntibodiesBased on the legacy o quality and innovation o Upstate, Chemicon and Calbiochem, EMD Millipores portolio o

    more than 10,000 puried, conjugated, anity-tagged, and enzyme-tagged primary antibodies, as well as secondaryantibodies with specicities or seven species, is widely published and trusted by researchers around the world. Your

    partner or epigenetics, cell signaling, cancer, neuroscience, toxicity, cell structure and stem cells, we provide you with

    the antibodies you need most. Generate reproducible results with antibodies that undergo rigorous quality control

    testing and are validated or Western blotting, immunoprecipitation, immunohistochemistry, ELISA, fow cytometry

    and more.

    *EMD Millipores Antibody Guarantee: Valid one year rom date

    o receipt. Does not apply to bulk, custom or commercial order.

    Subject to change without notice, and applies to validated

    antibody applications only.

    Positively the best place or your antibody

    research: www.millipore.com/antibodies

    For the entire amily o LentiBrite Lentiviral Biosensors, please visit www.millipore.com/cellstructure

    HeLa cells were transducedwith LentiBrite GFP-LC3lentiviral particles. TheGFP-LC3 displays a diusenuclear and cytosolicdistribution in ed cells(let), and a punctatedistribution in starvedautophagic cells (right).

    Reagents

    http://www.millipore.com/catalogue/item/17-10193?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/17-10189?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/17-10143?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/17-10188?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/antibodies/flx4/cell_structure?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/antibodies/flx4/cell_structure?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/antibodies/flx4/cell_structure?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/17-10188?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/17-10143?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/17-10189?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/17-10193?cid=BIOS-S-EPDF-1089-1207-DS
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    Millicell

    -Angiogenesis Assay KitThe Millicell -angiogenesis activation and inhibition

    assay kits provide a powerul, quantitative platorm or

    real-time monitoring o changes in tubule ormation

    with unprecedented optical resolution. The angiogenesis

    activation kit includes brinogen and thrombin

    components to make the brin gel, the activation control

    PMA (phorbol myristate acetate), and calcein-AM to

    visualize the cells in real time. The angiogenesis inhibition

    kit includes ECMatrix assays to induce angiogenesis,

    the potent angiogenesis inhibitor (D,L)-suloraphane toact as a control, and calcein-AM to visualize the cells in

    real time.

    MultiScreen Platesand 8-well Strips or

    Elispot AssaysFor decades, MultiScreen

    HTSlter plates with validated

    Immobilon-P (PVDF) membrane have been the industry

    standard or quantitating secreted proteins via Elispot

    assays. Validated Elispot assays are highly reliable and

    predictive. They can successully identiy individual

    antigen-specic T or B cells with results that mirror those

    obtained rom in vivostudies while eliminating the need

    or long-term in vitrocell cultures or the addition o

    exogenous cytokines.

    Description Catalogue No.

    Millicell -Angiogenesis Activation

    Assay Kit, 5 slides/kit

    MMA130

    Millicell -Angiogenesis Inhibition

    Assay Kit, 5 slides/kit

    MMA125

    Description Catalogue No.

    MultiScreenHTS

    IP, 0.45 m, opaque,non-sterile

    MSIPN4B10

    MultiScreenHTS

    IP, 0.45 m, clear,

    non-sterile

    MSIPN4510

    Single-well Punch Kit or individual

    membrane removal

    MELIPUNCH

    MultiScreenHTS

    IP, 0.45 m, 8-Well

    Strips, clear, sterile

    M8IPS4510

    MultiScreenHTS

    Support Frame M8IPFRAME

    KEY BENEFITS Unique slide design eliminates any liquid meniscus

    ormation and resulting out-o-ocus areas.

    Unprecedented visualization capabilities allow orcontinuous, live monitoring o cells as tubules orm.

    Full visualization o a well can be achieved at lowmagnication (10x), cutting down drastically on thetime needed to image each well.

    The all-in-one slide is used to grow, assay, and staincells and the miniaturized ormat reduces cost andwaste by up to 80%.

    KEY BENEFITS

    Dense, uniorm pore structure to promote antibody

    binding and increase sensitivity.

    Better, sharper spot denition provides superior results.

    Unique ability to process ewer than 96 samples at atime with our Immobilon-P 8-well strips, o whichEMD Millipore is the only supplier.

    Automation compatibility.

    Validated membranes.

    2To learn more, please visit www.millipore.com/cellculture

    To see more o the Elispot catalogue,

    please visit www.millipore.com/cellculture

    Assay Tools

    Image o typical Elispotwell. The spots are clear,ocused, and easy todistinguish.

    Photo courtesy of ZellNet

    Consulting, Inc.

    http://www.millipore.com/catalogue/item/MMA130?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MMA125?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MSIPN4B10?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MSIPN4510?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MELIPUNCH?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/M8IPS4510?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/M8IPFRAME?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/module/c78139?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/module/c78139?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/module/c78139?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/module/c78139?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/cellbiology/flx4/cell_culture?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/cellbiology/flx4/cell_culture?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/M8IPS4510?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MMA125?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MMA130?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/M8IPFRAME?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/M8IPS4510?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MELIPUNCH?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MSIPN4510?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MSIPN4B10?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/M8IPS4510?cid=BIOS-S-EPDF-1089-1207-DS
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    Assay Tools

    44

    AXIS AxonIsolation DeviceThe AXIS platorm is EMD Millipores most advanced

    tool or the study o neurite outgrowth. This slide-

    mounted microfuidic chamber system enables the

    deposition and culture o neural cells and the spatially

    controlled addition o growth actors, toxins, and other

    reagents. Neurite outgrowth is restricted to narrow,parallel channels, and the resultant outgrowth or collapse

    behavior is easily observed under a microscope. The

    result is a powerul platorm or the study o somas,

    neurites or synaptic ormation.

    KEY BENEFITS

    Optically clear device enables high resolutionmicroscopy, including live cell imaging, conocaland dierential intererence microscopy.

    Four dierent ormats provide test fexibility duringinvestigation.

    Inert, nontoxic polymer device enables cultures to bemaintained or weeks at a time.

    Description Catalogue No.

    AXIS Axon Isolation Device, 450 m AX45010

    AXIS Axon Isolation Device, 900 m AX90010TC

    Millicell -MigrationAssay KitThe Millicell -migration assay kit is a powerul,

    slide-based platorm that measures the eects o

    chemoattractants on the migration o adherent single

    cells through real-time imaging. Its microfuidic,

    low-volume technology promotes a stable, diusion-generated concentration gradient that is consistently

    linear and lasts or more than 48 hours. Designed

    or video microscopy assays, the -migration slide is

    made rom a plastic with high optical qualities similar

    to those o glass. At specic time intervals, you can

    acquire images o the observation area to achieve

    real-time monitoring and quantitative multiparametric

    measurements o cell migration. This innovative platorm

    results in high content data and analysis not achievable

    with Boyden chambers or wound healing assays.

    Description Catalogue No.

    Millicell -Migration Assay Kit, 4 slides

    (12 assays) per kit

    MMA205

    KEY BENEFITS

    Establishes a stable and linear concentration gradientater 1 hour that lasts 48 hours.

    Distinguish chemotaxis rom random movement.

    Multiparametric analysis enables greatermechanistic insight.

    Enhanced optical imaging or slow- andast-migrating cells.

    Analyze three chemoattractants in parallel orincreased throughput and analytical power.

    Ready to use, no assembly needed.

    : . .

    :

    ::

    : . .

    :

    ::

    300

    200

    100

    0

    -100

    -200

    -300

    -300 -200 -100 1000 200 300x axis [m]

    yaxis[m]

    Center o mass [m]: x=.097y=37.25

    Number o tracks: 80

    Counts up: 51

    Counts down: 29

    10% FCS

    To learn more, please visit www.millipore.com/cellculture

    http://www.millipore.com/catalogue/item/AX45010?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/AX90010TC?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MMA205?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/module/c78139?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/module/c78139?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/cellbiology/flx4/cell_culture?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/AX90010TC?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/AX45010?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MMA205?cid=BIOS-S-EPDF-1089-1207-DS
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    Assay Tools

    2

    Millicell and MultiScreenMultiwell PlatesNo matter what application youre working with,

    EMD Millipore has a plate developed to meet your

    specic needs. MultiScreen plates have a long history

    as a reliable tool or the advancement o drug discovery

    and lie science research. Applications include sample

    prep, receptor binding assays, solubility testing, PAMPA,

    PCR purication, protein concentration, neonatal

    screening, whole organism assays, and more. Millicell

    plates are well designed to accommodate the needs o

    drug transport and sodium fuorescein permeability

    assays, apoptosis, migration, telomerase activity and

    cell adhesion.

    KEY BENEFITS

    Simpliy handling o multiple samples simultaneously.

    Maintain assay integrity and prevent monolayerdisruption, contamination or damage.

    Assemblies include a choice o a multi-well orsingle-well eeder tray.

    Description Catalogue No.

    Millicell Plates

    Millicell-96 Cell Culture Insert Plate,

    polycarbonate, 0.4 m, 96-well receiver tray

    PSHT004S5

    Millicell-96 Cell Culture Insert Plate,

    polycarbonate, 0.4 m, single-well receiver tray

    PSHT004R5

    Millicell-24 Cell Culture Insert Plate,

    polyethylene terephthalate, 1.0 m

    PSRP010R5

    Millicell-24 Cell Culture Insert Plate,

    polycarbonate, 0.4 m

    PSHT010R5

    MultiScreen Plates

    MultiScreenhts IP, 0.45 m, 8-Well Strips,

    clear, sterile

    M8IPS4510

    MultiScreenhts IP, 0.45 m, clear, sterile MSIPS4510

    MultiScreen-HV, 0.45 m, clear, non-sterile MAHVN4550

    MultiScreenhts BV, 1.2 m, clear, non-sterile MSBVN1250

    Diusion ChamberKits & AccessoriesThe diusion chamber kits are proven devices or

    studying immunology, tissue growth, and organ

    transplants. They permit the growth o transplanted cells

    and tissues, while allowing nourishment by body fuids.

    The user can manipulate the access o macromolecules

    and non-resident cells to eectively inhibit antigenic

    reaction or rejection, thus enabling in vivoresearch.

    We also oer a variety o accessories such as a vacuum

    maniold, column loader, punch kit, and collectionplates and sealing tape.

    KEY BENEFITS

    Wide variety o membrane pore sizes enable fexibleexperimental design.

    Can be used with suspended cells or minced orwhole tissue.

    Includes membrane, glue, and Plexiglaschamber rings.

    Description Catalogue No.

    Durapore Membrane, PVDF, Hydrophilic,

    0.22 m, 13 mm, white, plain

    GVWP01300

    Durapore Membrane, PVDF, Hydrophilic,

    0.45 m, 13 mm, white, plain

    HVLP01300

    Durapore Membrane, PVDF, Hydrophilic,

    5.0 m, 13 mm, white, plain

    SVLP01300

    MF-Millipore Membrane, mixed cellulose

    esters, Hydrophilic, 0.22 m, 13 mm, white, plain

    GSWP01300

    MF-Millipore Membrane, mixed cellulose esters,

    Hydrophilic, 0.45 m, 13 mm, white, plain

    HAWP01300

    MF-Millipore Membrane, mixed cellulose esters,

    Hydrophilic, 5.0 m, 13 mm, white, plain

    SMWP01300

    2To learn more, please visit www.millipore.com/cellculture

    For more plate congurations, please visit www.millipore.com/cellculture

    http://www.millipore.com/catalogue/item/PSHT004S5?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PSHT004R5?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PSRP010R5?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PSHT010R5?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/M8IPS4510?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MSIPS4510?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MAHVN4550?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MSBVN1250?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/GVWP01300?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/HVLP01300?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SVLP01300?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/GSWP01300?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/HAWP01300?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SMWP01300?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/module/c78139?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/module/c78139?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/module/c78139?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/module/c78139?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/cellbiology/flx4/cell_culture?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/cellbiology/flx4/cell_culture?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SMWP01300?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/HAWP01300?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/GSWP01300?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/SVLP01300?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/HVLP01300?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/GVWP01300?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MSBVN1250?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MAHVN4550?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MSIPS4510?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/M8IPS4510?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PSHT010R5?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PSRP010R5?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PSHT004R5?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PSHT004S5?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/M8IPS4510?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MSBVN1250?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MAHVN4550?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/MSIPS4510?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/M8IPS4510?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PSHT010R5?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PSRP010R5?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PSHT004R5?cid=BIOS-S-EPDF-1089-1207-DShttp://www.millipore.com/catalogue/item/PSHT004S5?cid=BIOS-S-EPDF-1089-1207-DS
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    Scepter 2.0 Cell CounterWhile other automated counters consume bench

    space and rely on object recognition sotware, manual

    ocusing, and clumsy loading chambers, the Scepter

    cell counter provides true automation without the

    error that accompanies vision-based systems. With

    its microabricated, precision-engineered sensor, the

    Scepter cell counter does all the work and delivers

    accurate and reliable cell counts in less than 30 seconds.

    The reliability o Scepter counting is particularly

    apparent with smaller cell types. Because the Scepter

    cell counter measures volume using the Coulter Principle,

    it can quantiy cells based on size and will discriminate

    larger cells rom smaller debris unlike vision basedtechniques, which rely on object recognition sotware

    and cannot reliably detect small cells.

    Muse Cell AnalyzerThe Muse cell analyzer delivers accurate cell counts

    and quantitative assessments o cell health, apoptosis

    and cell cycle in just minutes, revolutionizing the way

    you analyze cells. By providing instant inormation onyour cell populations, the Muse cell analyzer enables

    you to make aster, more accurate decisions about your

    experiments, allowing or more productive research.

    Description Catalogue No.

    Scepter 2.0 Handheld Automated CellCounter with 40 m Scepter sensors (50/pk)

    PHCC20040

    Scepter 2.0 Handheld Automated Cell

    Counter with 60 m Scepter sensors (50/pk)

    PHCC20060

    KEY BENEFITS

    Accurately count every cell in your sample.

    Qualitatively determine cell health.

    Count subpopulations in heterogeneous samples,including Peripheral Blood Mononuclear Cells(PBMC) preps.

    Easily capture, analyze and manipulate data setswith Scepter Sotware Pro.

    KEY BENEFITS

    Accurate, fow-based detection delivers reliable results.

    Intuitive sotware and user-riendly touchscreenoperation.

    Rapid, one-step sample prep and analysis.

    6

    Description Catalogue No.

    Muse Cell Analyzer 0500-3115

    Muse Count & Viability Kit MCH100102

    Muse Annexin V & Dead Cell Kit MCH100105

    Learn more at www.millipore.com/scepter

    Learn more at www.millipore.com/muse

    Cell Analysis Platorms

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    Cell Analysis Platorms

    guava easyCyteFlow CytometryOur complete benchtop high throughput and single

    sample fow cytometry solution includes our instruments,assays, and sotware. The instruments contain up to 2

    lasers, and rom 58 parameters and fexible ormats or

    manual or automated walk-away loading. This complete

    end-to-end solution enables high quality results and

    ull service support. FlowCellect kits and Milli-Mark

    conjugated antibodies provide multiplexing capabilities,

    ease o use, require ewer incubation and wash steps,

    eature ull validation and concentration optimization,

    and are guaranteed to work in fow cytometry. Discover

    the power o fow cytometry or multiparametric analysis

    o cell health, cell cycle, mitochondria, apoptosis, stem

    cells, the immune system, and cell signaling.

    KEY BENEFITS

    Run minimal sample volumes without sheath fuid usingpatented microcapillary technology.

    View up to 8 cellular parameters in real timeor multiplexed analysis and easily comparedierent experiments.

    Use optimized assays to expedite results and avoidmanual compensation altogether.

    2

    Amnis FlowSight SystemAchieve fow cytometry with vision. This intuitive

    instrument operates like a conventional fow cytometer

    but provides imagery o every cell. Powerul and intuitive

    analysis sotware seamlessly links quantitative data

    to imagery. Whether in a base conguration or ully

    optioned, the FlowSight system sets a new standard

    o value.

    KEY BENEFITS

    Powerul enough or the core lab but sized and pricedor any lab.

    Unmatched sensitivity allows use o red dyes ordetection o dimly stained cells.

    Up to our lasers and 12 detection channelsenhance experimental fexibility and broadenyour application range.

    Additional upgrade options include 96-well plate

    AutoSampler and Quantitative Imaging.

    Learn more atwww.millipore.com/fowcytometry

    Learn more at www.amnis.com

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    To Place an Order or ReceiveTechnical Assistance

    In the U.S. and Canada, call toll-ree 1-800-645-5476

    For other countries across Europe and the world,

    please visit:www.emdmillipore.com/oces

    For Technical Service, please visit:

    www.emdmillipore.com/techservice

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    acebook.com/EMDMilliporeBioscience

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    EMD Millipore, the M mark, Scepter, Muse, guava easyCyte, 293-Free, RiboJuice,ProteoJuice, Sterivex, EpiGRO, LentiBrite, ECMatrix, AXIS, MF-Millipore and Millicoat aretrademarks o Merck KGaA, Darmstadt, Germany. Millicell, Stericup, Sterifip, Steritop,OmniPur, Fast-Trap, GeneJuice, NanoJuice, NovaCHOice, Millipore Express, Durapore, Millex,

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