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ACKNOWLEDGEMENTS OF CORES IN PUBLICATIONS Scientific publications containing data or other appropriate support using Core services should include the following statement in the ack- nowledgment section: This project was supported by the “fill in full name” Core at BCM and the expert assistance of the Core Director(s) “fill in name.” CLICK TO LEARN MORE ABOUT A SPECIFIC CORE LAB Bioinformatics Research Laboratory Core (BRL) Cytometry and Cell Sorting DNA Sequencing Genetically Engineered Mouse (GEM) Genome-wide shRNA Screening (GRSA/ C-BASS) Genomic and RNA Profiling (GARP) Human Tissue Acquisition and Pathology (HTAP) Integrated Microscopy Mass Spectrometry Proteomics MHC Tetramer Monoclonal Antibody/Recombinant Protein Expression Mouse Embryonic Stem Cells Mouse Metabolic Research Unit (Children’s Nutrition Research Center) Mouse Phenotyping Optical Imaging and Vital Microscopy (OIVM) Pathway Discovery Proteomics Population Sciences Biorepository (PSB) Protein and Antibody Array Proteomics RNA In Situ Hybridization The Mouse Embryonic Stem Cells Core is excited to announce that it is now under the leadership of Jason Heaney, Ph.D., Assistant Professor in the Department of Molecular and Human Genetics, who will be bringing his years of experience with ES Cell culture and transgenic mouse technologies to the core as its Academic Director. During the next year the core will be expanding or introducing several services. First, the core will be expanding its capacity for targeting transgenes to the Hprt locus in ES cells, which permits tissue specific expression of transgenes without the drawbacks of random integration. Second, the core will be introducing CRISPR/Cas9 services for in vivo genome editing of model organisms. The core will provide tiers of service including consultations on sgRNA design and selection, wild-type or nickase Cas9 approaches, the design of vector and oligo for homology directed repair, and genotyping assays; production of sgRNA and Cas9 DNA templates; and in vitro transcription of sgRNAs and Cas9. For mouse and rat genome editing, the core will hand off RNAs to the Genetically Engineered Mouse (GEM) Core for zygote injections and animal production. We look forward to working with the BCM research community on these exciting technologies. For more information please email [email protected] We are pleased to announce that CRISPR/Cas9 genomic editing is now being offered as services by established Institutional Advanced Technology Cores. The Mouse Embryonic Stem Cell Core, Directed by Dr. Jason Heaney, and the Genetically Engineered Mouse (GEM) Core, Directed by Dr. Franco DeMayo, together will provide comprehensive services for in vivo genome editing of model organisms. The Cell-Based Assay Screening Service (C-BASS) Core Directed by Drs. Trey Westbrook and Dan Liu will provide in vitro genomic editing services with somatic cell cultures. ADVANCED TECHNOLOGY CORES JUNE 2014 MOUSE EMBRYONIC STEM CELL CORE CELL-BASED ASSAY SCREENING SERVICE (C-BASS) Nuclease-based genome editing tool CRISPR/Cas9 enables complete inactivation of endogenous genes, and have joined RNAi as methods of choice for loss-of-function studies, rapidly becoming an essential part of the genomic research arsenal. To (continued on next page)
