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Paul Reibach, Ph.D.

Smithers Viscient.

UsingTheScientificMethod

Wicked Cool Autumn

Adventures in Chromatography

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Scientific Method

1) Observe some aspect of the universe.

2) Develop a tentative description called a hypothesisthat is consistent !ith !hat you have observed.

") #se the hypothesis to make predictions.

$) %est those predictions by e&periments or further

observations and modify the hypothesis based on yourresults.

') (epeat steps " and $ until there are no discrepanciesbet!een hypothesis and e&periment andorobservation.

http://phyun5.ucr.edu/~wudka/Physics7/Notes_www/node6.html

%he scientific method is the best !ay yet discovered

for separating scientific truths from falsehoods and

delusions. A simple version looks something like this

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Color

What is color*

What is light*

What is a pigment*

What causes colors in markers*

Can !e analy+e the pigments*

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Colors

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Light

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Pigments

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Marker Pigments

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!hromatography!hromatography was "rst employed #y $ussianscientist Mikhail %s&et in '()). *e worked withchromatography in the "rst decade o+ the ,)thcentury- primarily +or the separation o+ plant

pigments such as chlorophyll- carotenes- andanthophylls. ince these components ha&edi0erent colors 1green- orange- and yellow-respecti&ely2 they ga&e the techni3ue its name.

!hromatography 1+rom 4reek 89 chromacolor and ;?@ graphein to write is thecollecti&e term +or a set o+ la#oratory techni3ues+or the separation o+ mitures.

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!hromatography

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Scientific Method

1) Observation and description of a phenomenon or roupof phenomena.

!) "ormulation of a h#pothesis to e$plain the phenomena.

%) &se of the h#pothesis to predict the e$istence of otherphenomena, or to predict 'uantitativel# the results of ne(

observations.

) Performance of e$perimental tests of the predictions b#

several independent e$perimenters and properl#performed e$periments.

http://teacher.nsrl.rochester.edu/phy_la#s/appendiA/html

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Marker colors are the result

o+ a miture o+ di0erentpigments.

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*#pothesisChromatoraph# is a tool used to

separate various chemicals from

each other.Various mar+er colors are due to a

mi$ture of piments.

Chromatoraph# can be used to

separate the piment mi$tures.

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oda#-s $periment

Observe Some Phenomenon

*#pothesis

Ma+e Predictions$periment Materials

Procedure

Results

Conclusion

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$periment

Materials/

1. "ilter paper

!. Plastic or paper cups

%. 0ater soluble mar+ers Cra#ola

(ashable mar+ers or other (ashable

mar+ers)

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Procedure'. Mark your name or initials near the edge o+ the paper BN PAN!BL.

,. Craw a circle in the middle using a 3uarter or "lm can.

D. Poke a small round hole 1smaller than a dime2 in the center o+ thepaper.

E. Fse markers and a $eeseGs Piece to make small dots o+ dye on the ring.

5. %his paper will #ecome your chromatogram.6. %o make a wick- roll a second "lter paper into a cylinder

7. Bnsert this rolled wick into the round hole in your chromatogram.

H. Iill the cup hal+ +ull o+ tap water.

(. Lay the chromatogram with the wick etending down into the water

'). Jatch your chromatogram- as the water carries the ink as it tra&els.

''. K#ser&e what happens to the pigments.',. Jhen the water nearly reaches the edge remo&e it and dry.

'D. Ciscard the wick into the waste #asket.

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Results

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Conclusion

he e$periment supported m#

h#pothesis

he e$periment did not support

m# h#pothesis

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