Affect of DNA-PK on Foci Persistence

J. Dickey

J Cell Controls (0 Gy)0 hr

24 hr

48 hr

72 hr

K Cell Controls (0 Gy)0 hr

24 hr

48 hr

72 hr

J Cells 3 Gy

1 hr 3 hr 6 hr

10 hr 24 hr

0.5 hr

48 hr 72 hr

K Cells 3 Gy1 hr 3 hr 6 hr

10 hr 24 hr

0.5 hr

48 hr 72 hr

J Cells 5 Gy

1 hr 3 hr 6 hr

10 hr 24 hr

0.5 hr

48 hr 72 hr

K Cells 5 Gy1 hr 3 hr 6 hr

10 hr 24 hr

0.5 hr

48 hr 72 hr

J vs. K cells 10 hr post 3 Gy IR

J Cells K Cells

J vs. K cells 10 hr post 5 Gy IR

J Cells K Cells

Conclusions

• J cells (deficient in DNA-PK expression) have greater foci persistence over time after IR.

• Overall numbers of foci between J cells and K cells at 30 min post-IR seem similar.

• DNA-PK seems to play a role in foci removal.