Agenda  

§  Introduc)on  to    (5  mins)  

§  Hands  on  workflows  in  HLA  Twin  (15  mins)  §  High  Quality  Holotype  HLA  X4  data  §  Novel  alleles  with  IMGT/HLA  v3.10  

§  Export  consensus  

§  Novel  alleles  with  IMGT/HLA  v3.15  

§  Q&A!  (10  mins)  

Omixon  HLA  Twin™  (So4ware)  

§  SoPware  component  of  Holotype  HLA  

§  Automated  genotyping  aPer  MiSeq  run  

§  Preconfigured  Holotype  HLA-­‐specific  seVngs  

§  Two  algorithms  for  determining  HLA  genotypes  –  Consensus  Genotyping  (Assembly)    –  Sta)s)cal  Genotyping  (Alignment  to  IMGT/HLA)  

§  Traffic  light  system  for  data  interpreta)on  and  workflow  management  

PASSED  

WARNING  

FAILED  

3  IT  Implementa<ons  of  Twin  

Manual  launch  on  a  Server  

Fully  automated  on  a  Server  

HLA  Twin  on  a  laptop  or  PC  

Summary  cards  

View  bu_on  

Sample  Details  

Browse  Alignment  

Naviga)on  bu_ons  

Traffic  Light  #1  Dual  Algorithm  Concordance  

Fully  concordant  

Concordant  to  2nd  field  

Concordant  to  1st  field  

Discordant  

Traffic  Light  #2  Locus-­‐level  Quality  Check  

All  QC  metrics  passed  

At  least  1  QC  warning  

At  least  1  QC  failed  

2  Hands  on  Workflows  in  Twin    

§  High  Quality  Holotype  HLA  X4  data  –  5  loci  +  1  pool  sequenced  independently  per  sample  

§  Novel  Allele  Discovery  –  Discover  novel  allele  with  IMGT/HLA  3.10  

–  Export  consensus  –  Resolve  novel  allele  by  reanalyzing  with  IMGT/HLA  3.15