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Algal Control By Phosphate Restriction Mason Lake (Irvine) contains toxic algae. They will install...

Date post: 31-Dec-2015
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Algal Control By Phosphate Restriction Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate. Newport Back Bay is nearby and may also be contaminated with the toxic algae. Eastlake (Yorba Linda) is the control lake and is not being treated.
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Page 1: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

Algal Control By Phosphate Restriction

Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.

Newport Back Bay is nearby and may also be contaminated with the toxic algae.

Eastlake (Yorba Linda) is the control lake and is not being treated.

Page 2: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

Mason Lake

Sampling Site

Page 3: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

Toxic Algae in Mason Lake

Page 4: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

Visibility: 4cm

Page 5: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

High phosphate levels are from many water fowl

Page 6: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

Toxin-Producing Microcystis sp.

Page 7: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

Mason Lake: One Mile From Newport Back Bay Estuary

Page 8: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

Newport Back Bay

Page 9: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

Urban runoff into the bay

Bay

Page 10: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

Storm drains with Phosphate-rich water from urban run-off

Page 11: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

Phosphate-rich water dumping into the Bay

Page 12: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

Upper Newport Bay

Top birding site Home to 200 species of birds 30,000 migrating birds per day Audubon Society/F&G conducts a

monthly bird count

Page 13: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

Endangered Species at Newport Back Bay: California Least Tern

Page 14: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

Light-Footed Clapper Rail

Page 15: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

Brown Pelican

Page 16: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

Peregrine Falcon

Page 17: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

Belding’s Savannah Sparrow

Page 18: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

Coastal California Gnatcatcher

Page 19: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

East Lake (Yorba Linda)Sampling Site

Page 20: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

East Lake: Yorba Linda, CA

Page 21: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

Algae is creating a foamy surface scum

Page 22: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

A Diatom called Navicula is causing the foam

Page 23: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

Sample Analysis

Algal Assay Species ID Cell count

Chlorophyll Assay Flow Cytometry

Species populations Phosphate Assay

Page 24: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

Algal Assay

Page 25: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

Eastlake: Diatoms

Page 26: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

Mason Lake: Cyanobacteria

Page 27: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

Flow Cytometry is used to count algal cells and separate them by species

Page 28: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

Flow Cytometry is used to count algal cells and separate them by species

Page 29: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

Chlorophyll Assay

Filter the water to capture the algae on the filter paper

Page 30: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

Mason Lake Eastlake

Page 31: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

Grind up filter paper with acetone to release chlorophyll

Page 32: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

After Centrifuge

Page 33: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

Filter algae out of water, add a reagent that turns blue when it contacts phosphate

Phosphate Assay

Page 34: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

Mason Lake Eastlake

Filters with algae left on them

Page 35: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

The deeper the blue color, the

more phosphate is present.

Page 36: Algal Control By Phosphate Restriction  Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.  Newport.

A dilution series with phosphate standard


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