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AMBION, INC. RETROscript ® Catalog #1710 M-MLV RT Material Safety Data Sheet Version 1 Page 1 of 3 Printed: 12/23/2005 Created:070104 QC Form: 1522 Revision Date: N.A Section 1-Identification of Product 2043G WHMIS Classification Health: 1 Fire: 1 Stability: 0 Contact Information Ambion, Inc 2130 Woodward St. Austin, TX 78744-1832 Tel: +1 512 651 0200 US Toll-free Tel: 800 888 8804 E- mail: [email protected] Web address www.ambion.com Ambion (Europe) LTD Huntingdon, Cambidgeshire UK PE29 6XY Tel:+44 (0)1480 373 020 Fax: +44 (0)1480 373 010 E-mail: [email protected] Web address: www.ambion.com Emergency Contact: In Europe, call 112. In USA, call 911 Section 2-Composition/Information Hazardous Ingredients (Specific) % CAS # LD 50 LC 50 Glycerol 50 56-81-5 Oral-Rat LD50: 12,600 mg/kg Inhalation, rat: >570 mg/m3/1H Section 3-Hazard Identification Routes of Entry Skin Contact Causes skin irritation Skin Absorption Causes skin irritation Eye Contact Causes eye irritation Inhalation Causes respiratory irritation Ingestion Causes gastrointestinal irritation [Emergency Overview] Caution! May cause eye and skin irritation. May cause respiratory and digestive tract irritation. This is expected to be a low hazard for usual industrial handling. WHMIS Symbols N.A. [Potential Health Effects] Poison by subcutaneous route. Mildly toxic by ingestion. Human systemic effects by ingestion: headache and nausea or vomiting. Experimental reproductive effects. Human mutation data reported. A skin and eye irritant. In the form of mist it is a nuisance particulate and inhalation irritant. Combustible liquid when exposed to heat, flame, or powerful oxidizers. Mixtures with hydrogen peroxide are highly explosive. Ignites on contact with potassium permanganate, calcium hypochlorite. Mixture with nitric acid + sulfuric acid forms the explosive glyceryl nitrate. Mixture with perchloric acid + lead oxide forms explosive perchlorate esters. Confined mixture with chlorine explodes if heated to 70–80º. Can react violently with acetic anhydride, aniline + nitrobenzene, Ca(OCl)2, CrO3, Cr2O3, F2 + PbO, phosphorus triiodide, ethylene oxide + heat, KMnO4, K2O2, AgClO4, Na2O2, NaH. Energetic reaction with sodium hydride. Mixture with nitric acid + hydrofluoric acid is a storage hazard due to gas evolution. To fight fire, use alcohol foam, CO2, dry chemical. When heated to decomposition it emits acrid smoke and fumes. Section 4-First Aid Measures Skin Contact Flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Get medical aid if irritation develops or persists. Wash clothing before reuse. Eye Contact Flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. If irritation develops, get medical aid. Inhalation Remove from exposure and move to fresh air immediately. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical aid if cough or other symptoms appear. Ingestion Never give anything by mouth to an unconscious person. Do NOT induce vomiting. If conscious and alert, rinse mouth and drink 2-4 cupfuls of milk or water. Get medical aid if irritation or symptoms occur. Section 5-Fire Fighting Measures Flammable N.A Means of Extinction Use water spray to cool fire-exposed containers. Use agent most appropriate to extinguish fire. Use water spray, dry chemical, carbon dioxide, or appropriate foam. Flashpoint ( o C) and Method N.A Upper Flammable Limit (% by volume) N.A Lower Flammable Limit (% by volume) N.A
Transcript

AMBION, INC. RETROscript® Catalog #1710 M-MLV RT Material Safety Data Sheet

Version 1 Page 1 of 3 Printed: 12/23/2005 Created:070104 QC Form: 1522 Revision Date: N.A

Section 1-Identification of Product 2043G WHMIS Classification

Health: 1 Fire: 1 Stability: 0

Contact Information Ambion, Inc 2130 Woodward St. Austin, TX 78744-1832 Tel: +1 512 651 0200 US Toll-free Tel: 800 888 8804 E-mail: [email protected] Web address www.ambion.com Ambion (Europe) LTD Huntingdon, Cambidgeshire UK PE29 6XY Tel:+44 (0)1480 373 020 Fax: +44 (0)1480 373 010 E-mail: [email protected] Web address: www.ambion.com Emergency Contact: In Europe, call 112. In USA, call 911 Section 2-Composition/Information Hazardous Ingredients (Specific) % CAS # LD50 LC50 Glycerol 50 56-81-5 Oral-Rat

LD50: 12,600 mg/kg

Inhalation, rat: >570 mg/m3/1H

Section 3-Hazard Identification Routes of Entry

Skin Contact Causes skin irritation Skin Absorption Causes skin irritation Eye Contact Causes eye irritation Inhalation Causes respiratory irritation Ingestion Causes gastrointestinal irritation

[Emergency Overview] Caution! May cause eye and skin irritation. May cause respiratory and digestive tract irritation. This is expected to be a low hazard for usual industrial handling. WHMIS Symbols N.A. [Potential Health Effects] Poison by subcutaneous route. Mildly toxic by ingestion. Human systemic effects by ingestion: headache and nausea or vomiting. Experimental reproductive effects. Human mutation data reported. A skin and eye irritant. In the form of mist it is a nuisance particulate and inhalation irritant. Combustible liquid when exposed to heat, flame, or powerful oxidizers. Mixtures with hydrogen peroxide are highly explosive. Ignites on contact with potassium permanganate, calcium hypochlorite. Mixture with nitric acid + sulfuric acid forms the explosive glyceryl nitrate. Mixture with perchloric acid + lead oxide forms explosive perchlorate esters. Confined mixture with chlorine explodes if heated to 70–80º. Can react violently with acetic anhydride, aniline + nitrobenzene, Ca(OCl)2, CrO3, Cr2O3, F2 + PbO, phosphorus triiodide, ethylene oxide + heat, KMnO4, K2O2, AgClO4, Na2O2, NaH. Energetic reaction with sodium hydride. Mixture with nitric acid + hydrofluoric acid is a storage hazard due to gas evolution. To fight fire, use alcohol foam, CO2, dry chemical. When heated to decomposition it emits acrid smoke and fumes. Section 4-First Aid Measures Skin Contact Flush skin with plenty of water for at least 15 minutes while removing contaminated clothing

and shoes. Get medical aid if irritation develops or persists. Wash clothing before reuse. Eye Contact Flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower

eyelids. If irritation develops, get medical aid. Inhalation Remove from exposure and move to fresh air immediately. If not breathing, give artificial

respiration. If breathing is difficult, give oxygen. Get medical aid if cough or other symptoms appear.

Ingestion Never give anything by mouth to an unconscious person. Do NOT induce vomiting. If conscious and alert, rinse mouth and drink 2-4 cupfuls of milk or water. Get medical aid if irritation or symptoms occur.

Section 5-Fire Fighting Measures Flammable N.A Means of Extinction Use water spray to cool fire-exposed containers. Use agent

most appropriate to extinguish fire. Use water spray, dry chemical, carbon dioxide, or appropriate foam.

Flashpoint (oC) and Method N.A Upper Flammable Limit (% by volume) N.A Lower Flammable Limit (% by volume) N.A

AMBION, INC. RETROscript® Catalog #1710 M-MLV RT Material Safety Data Sheet

Version 1 Page 2 of 3 Printed: 12/23/2005 Created:070104 QC Form: 1522 Revision Date: N.A

Autoignition Temperature (oC) N.A Explosion Data – Sensitivity to Impact N.A Explosion Data – Sensitivity to Static Discharge N.A Hazardous Combustion Products Carbon monoxide, irritating and toxic fumes and gases,

carbon dioxide. NFPA Health: 1 Fire: 1 Stability: 0 Section 6-Accidental Release Measures Leak and Spill Procedures Clean up spills immediately, observing precautions in the Protective Equipment section. Sweep up or absorb material, then place into a suitable clean, dry, closed container for disposal. Section 7-Handling and Storage Handling Procedures and Equipment

Wash thoroughly after handling. Wash hands before eating. Use with adequate ventilation. Avoid contact with eyes, skin, and clothing. Keep container tightly closed. Avoid ingestion and inhalation. Wash clothing before reuse

Storage Requirements Keep in a tightly closed container, stored in a cool, dry, ventilated area. Protect against physical damage. Isolate from incompatible substances. Containers of this material may be hazardous when empty since they retain product residues (dust, solids); observe all warnings and precautions listed for the product.

Section 8-Exposure Control/Personal Protection Exposure Limits ACGIH TLV 10 mg/m3 TWA OSHA PEL 15 mg/m3 TWA (total dust); 5 mg/m3 TWA (respirable fraction) Other (specify) N.A. Engineering Controls (specific)

General Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower. Use adequate ventilation to keep airborne concentrations low

Local Exhaust A system of local and/or general exhaust is recommended to keep employee exposures as low as possible. Local exhaust ventilation is generally preferred because it can control the emissions of the contaminant at its source, preventing dispersion of it into the general work area. Please refer to the ACGIH document, Industrial Ventilation, A Manual of Recommended Practices, most recent edition, for details

Other N.A. Personal Protective Equipment (specific)

Gloves Synthetic Gloves Respirator A respiratory protection program that meets OSHA's 29 CFR 1910.134 and

ANSI Z88.2 requirements or European Standard EN 149 must be followed whenever workplace conditions warrant a respirator's use.

Eye Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166. Maintain eye wash fountain and quick-drench facilities in work area

Footwear Closed toe shoes Clothing Wear appropriate protective clothing to prevent skin exposure. Other

Section 9-Physical and Chemical Properties Physical State Liquid Odor and Appearance Clear Odor Threshold (ppm) Not Available Specific Gravity Not Available Vapor Density (Air=1) Not Available Vapor Pressure (mmHg) Not Available Evaporation Rate Not Available Boiling Point (oC) Not Available Freezing Point (oC) Not Available pH Not Available Coefficient of Water/Oil Distribution Not Available

AMBION, INC. RETROscript® Catalog #1710 M-MLV RT Material Safety Data Sheet

Version 1 Page 3 of 3 Printed: 12/23/2005 Created:070104 QC Form: 1522 Revision Date: N.A

[Solubility in Water] Not Available Section 10-Stability and Reactivity Chemical Stability Stable Incompatible with other substances Acetic anhydride, potassium permanganate, strong acids, caustics

(e.g. ammonia, ammonium hydroxide, calcium hydroxide, potassium hydroxide, sodium hydroxide), isocyanates, oxidizing agents, aliphatic amines

Reactivity Not Available Hazardous Decomposition Products Carbon monoxide, irritating and toxic fumes and gases, carbon

dioxide. Section 11-Toxicological Information

Acute Effects Not Tested Chronic Effects Not Tested Irritancy of Product Not Tested Skin Sensitization Not Tested Respiratory Sensitization Not Tested

Carcinogenicity IARC (1,2A, or 2B) Not Listed ACGIH (A1, A2, or A3) Not Listed Reproductive Toxicity Not Listed Teratogenicity Not Listed Embryotoxicity Not Listed Mutagenicity Not Listed Name of Synergistic Products/Effects

Not Listed

Section 12-Ecological Information Aquatic Toxicity LC50 (96 Hr.) rainbow trout = 50-67 mg/L; 12 degrees CLC50 (96 Hr.) goldfish

= >5000 mg/L Section 13-Disposal Considerations

Waste Disposal Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste. US EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3. Additionally, waste generators must consult state and local hazardous waste regulations to ensure complete and accurate classification.

Section 14-Transport Information Special Shipping Information

PIN Not Listed TDG Not Listed [DOT] Not Listed [IMO] Not Listed [ICAO] Not Listed

Section 15-Regulatory Information [WHMIS Classification] Not Listed [OSHA] Not Listed [SARA] CAS # 56-81-5: chronic [TSCA] Listed

Section 16-Other Information This bulletin is for your guidance and is based upon information and tests believed to be reliable. Ambion makes no guarantee of the accuracy or completeness of the data and shall not be liable for any damages thereto. The data are offered solely for your consideration, investigation, and verification. These suggestions should not be confused with state, municipal, or insurance requirements, or with national safety codes and constitute no warranty. Any use of these data and information must be determined by the user to be in accordance with applicable federal, state, and local regulations.

AMBION, INC. RETROscript® Catalog #1710 RNase Inhibitor Material Safety Data Sheet

Version 1 Page 1 of 3 Printed: 12/23/2005 Created:070104 QC Form: 1523 Revision Date: N.A

Section 1-Identification of Product 2687G WHMIS Classification

Health: 1 Fire: 1 Stability: 0

Contact Information Ambion, Inc 2130 Woodward St. Austin, TX 78744-1832 Tel: +1 512 651 0200 US Toll-free Tel: 800 888 8804 E-mail: [email protected] Web address www.ambion.com Ambion (Europe) LTD Huntingdon, Cambidgeshire UK PE29 6XY Tel:+44 (0)1480 373 020 Fax: +44 (0)1480 373 010 E-mail: [email protected] Web address: www.ambion.com Emergency Contact: In Europe, call 112. In USA, call 911 Section 2-Composition/Information Hazardous Ingredients (Specific) % CAS # LD50 LC50 Glycerol 50 56-81-5 Oral-Rat

LD50: 12,600 mg/kg

Inhalation, rat: >570 mg/m3/1H

Section 3-Hazard Identification Routes of Entry

Skin Contact Causes skin irritation Skin Absorption Causes skin irritation Eye Contact Causes eye irritation Inhalation Causes respiratory irritation Ingestion Causes gastrointestinal irritation

[Emergency Overview] Caution! May cause eye and skin irritation. May cause respiratory and digestive tract irritation. This is expected to be a low hazard for usual industrial handling. WHMIS Symbols N.A. [Potential Health Effects] Poison by subcutaneous route. Mildly toxic by ingestion. Human systemic effects by ingestion: headache and nausea or vomiting. Experimental reproductive effects. Human mutation data reported. A skin and eye irritant. In the form of mist it is a nuisance particulate and inhalation irritant. Combustible liquid when exposed to heat, flame, or powerful oxidizers. Mixtures with hydrogen peroxide are highly explosive. Ignites on contact with potassium permanganate, calcium hypochlorite. Mixture with nitric acid + sulfuric acid forms the explosive glyceryl nitrate. Mixture with perchloric acid + lead oxide forms explosive perchlorate esters. Confined mixture with chlorine explodes if heated to 70–80º. Can react violently with acetic anhydride, aniline + nitrobenzene, Ca(OCl)2, CrO3, Cr2O3, F2 + PbO, phosphorus triiodide, ethylene oxide + heat, KMnO4, K2O2, AgClO4, Na2O2, NaH. Energetic reaction with sodium hydride. Mixture with nitric acid + hydrofluoric acid is a storage hazard due to gas evolution. To fight fire, use alcohol foam, CO2, dry chemical. When heated to decomposition it emits acrid smoke and fumes. Section 4-First Aid Measures Skin Contact Flush skin with plenty of water for at least 15 minutes while removing contaminated clothing

and shoes. Get medical aid if irritation develops or persists. Wash clothing before reuse. Eye Contact Flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower

eyelids. If irritation develops, get medical aid. Inhalation Remove from exposure and move to fresh air immediately. If not breathing, give artificial

respiration. If breathing is difficult, give oxygen. Get medical aid if cough or other symptoms appear.

Ingestion Never give anything by mouth to an unconscious person. Do NOT induce vomiting. If conscious and alert, rinse mouth and drink 2-4 cupfuls of milk or water. Get medical aid if irritation or symptoms occur.

Section 5-Fire Fighting Measures Flammable N.A Means of Extinction Use water spray to cool fire-exposed containers. Use agent

most appropriate to extinguish fire. Use water spray, dry chemical, carbon dioxide, or appropriate foam.

Flashpoint (oC) and Method N.A Upper Flammable Limit (% by volume) N.A Lower Flammable Limit (% by volume) N.A

AMBION, INC. RETROscript® Catalog #1710 RNase Inhibitor Material Safety Data Sheet

Version 1 Page 2 of 3 Printed: 12/23/2005 Created:070104 QC Form: 1523 Revision Date: N.A

Autoignition Temperature (oC) N.A Explosion Data – Sensitivity to Impact N.A Explosion Data – Sensitivity to Static Discharge N.A Hazardous Combustion Products Carbon monoxide, irritating and toxic fumes and gases,

carbon dioxide. NFPA Health: 1 Fire: 1 Stability: 0 Section 6-Accidental Release Measures Leak and Spill Procedures Clean up spills immediately, observing precautions in the Protective Equipment section. Sweep up or absorb material, then place into a suitable clean, dry, closed container for disposal. Section 7-Handling and Storage Handling Procedures and Equipment

Wash thoroughly after handling. Wash hands before eating. Use with adequate ventilation. Avoid contact with eyes, skin, and clothing. Keep container tightly closed. Avoid ingestion and inhalation. Wash clothing before reuse

Storage Requirements Keep in a tightly closed container, stored in a cool, dry, ventilated area. Protect against physical damage. Isolate from incompatible substances. Containers of this material may be hazardous when empty since they retain product residues (dust, solids); observe all warnings and precautions listed for the product.

Section 8-Exposure Control/Personal Protection Exposure Limits ACGIH TLV 10 mg/m3 TWA OSHA PEL 15 mg/m3 TWA (total dust); 5 mg/m3 TWA (respirable fraction) Other (specify) N.A. Engineering Controls (specific)

General Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower. Use adequate ventilation to keep airborne concentrations low

Local Exhaust A system of local and/or general exhaust is recommended to keep employee exposures as low as possible. Local exhaust ventilation is generally preferred because it can control the emissions of the contaminant at its source, preventing dispersion of it into the general work area. Please refer to the ACGIH document, Industrial Ventilation, A Manual of Recommended Practices, most recent edition, for details

Other N.A. Personal Protective Equipment (specific)

Gloves Synthetic Gloves Respirator A respiratory protection program that meets OSHA's 29 CFR 1910.134 and

ANSI Z88.2 requirements or European Standard EN 149 must be followed whenever workplace conditions warrant a respirator's use.

Eye Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166. Maintain eye wash fountain and quick-drench facilities in work area

Footwear Closed toe shoes Clothing Wear appropriate protective clothing to prevent skin exposure. Other N.A.

Section 9-Physical and Chemical Properties Physical State Liquid Odor and Appearance Clear Odor Threshold (ppm) Not Available Specific Gravity Not Available Vapor Density (Air=1) Not Available Vapor Pressure (mmHg) Not Available Evaporation Rate Not Available Boiling Point (oC) Not Available Freezing Point (oC) Not Available pH Not Available Coefficient of Water/Oil Distribution Not Available

AMBION, INC. RETROscript® Catalog #1710 RNase Inhibitor Material Safety Data Sheet

Version 1 Page 3 of 3 Printed: 12/23/2005 Created:070104 QC Form: 1523 Revision Date: N.A

[Solubility in Water] Not Available Section 10-Stability and Reactivity Chemical Stability Stable Incompatible with other substances Acetic anhydride, potassium permanganate, strong acids, caustics

(e.g. ammonia, ammonium hydroxide, calcium hydroxide, potassium hydroxide, sodium hydroxide), isocyanates, oxidizing agents, aliphatic amines

Reactivity Not Available Hazardous Decomposition Products Carbon monoxide, irritating and toxic fumes and gases, carbon

dioxide. Section 11-Toxicological Information

Acute Effects Not Tested Chronic Effects Not Tested Irritancy of Product Not Tested Skin Sensitization Not Tested Respiratory Sensitization Not Tested

Carcinogenicity IARC (1,2A, or 2B) Not Listed ACGIH (A1, A2, or A3) Not Listed Reproductive Toxicity Not Listed Teratogenicity Not Listed Embryotoxicity Not Listed Mutagenicity Not Listed Name of Synergistic Products/Effects

Not Listed

Section 12-Ecological Information Aquatic Toxicity LC50 (96 Hr.) rainbow trout = 50-67 mg/L; 12 degrees CLC50 (96 Hr.) goldfish

= >5000 mg/L Section 13-Disposal Considerations

Waste Disposal Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste. US EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3. Additionally, waste generators must consult state and local hazardous waste regulations to ensure complete and accurate classification.

Section 14-Transport Information Special Shipping Information

PIN Not Listed TDG Not Listed [DOT] Not Listed [IMO] Not Listed [ICAO] Not Listed

Section 15-Regulatory Information [WHMIS Classification] Not Listed [OSHA] Not Listed [SARA] CAS # 56-81-5: chronic [TSCA] Listed

Section 16-Other Information This bulletin is for your guidance and is based upon information and tests believed to be reliable. Ambion makes no guarantee of the accuracy or completeness of the data and shall not be liable for any damages thereto. The data are offered solely for your consideration, investigation, and verification. These suggestions should not be confused with state, municipal, or insurance requirements, or with national safety codes and constitute no warranty. Any use of these data and information must be determined by the user to be in accordance with applicable federal, state, and local regulations.

Version# 1 Revision Date: N.A. Page 1 of 1 Printed:12/23/2005 Created Date:070104 QC Form 1526

SAFETY STATEMENT This document is only available in English. 1. Identification of the substance/preparation and company/undertaking

Product/Kit Name: RETROscript® Catalog #: 1710 Product/Component Name: Random Decamers

Catalog Number: 5722G

Supplier: Ambion, Inc.

Emergency Telephone Numbers:

USA: 1-800-888-8804

Europe: +44 (0) 1480 373 020

2. Composition/ information on ingredients Substance/Preparation: To the present knowledge of Ambion, Inc., this product does not contain any hazardous ingredients in quantities requiring reporting in this section, in accordance with EU regulations or National regulations.

To Ambion’s knowledge this substance/preparation is not classified as hazardous according to EU, US, or any other known national regulations.

9. Physical and chemical properties

Physical State: Liquid Color: Colorless 16. Other information:

To the best of Ambion’s knowledge, the information contained herein is accurate. However, Ambion, Inc. does not assume any liability whatsoever for the accuracy or completeness of the information contained herein. Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used with caution.

This is not an MSDS. According to EU and US regulations we are not required to supply an MSDS for a product, which is not classified as hazardous.

Version# 1 Revision Date: N.A. Page 1 of 1 Printed:12/23/2005 Created Date:070104 QC Form 1527

SAFETY STATEMENT This document is only available in English. 1. Identification of the substance/preparation and company/undertaking

Product/Kit Name: RETROscript® Catalog #: 1710 Product/Component Name: Oligo(dT) Primers

Catalog Number: 5730G

Supplier: Ambion, Inc.

Emergency Telephone Numbers:

USA: 1-800-888-8804

Europe: +44 (0) 1480 373 020

2. Composition/ information on ingredients Substance/Preparation: To the present knowledge of Ambion, Inc., this product does not contain any hazardous ingredients in quantities requiring reporting in this section, in accordance with EU regulations or National regulations.

To Ambion’s knowledge this substance/preparation is not classified as hazardous according to EU, US, or any other known national regulations.

9. Physical and chemical properties

Physical State: Liquid Color: Colorless 16. Other information:

To the best of Ambion’s knowledge, the information contained herein is accurate. However, Ambion, Inc. does not assume any liability whatsoever for the accuracy or completeness of the information contained herein. Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used with caution.

This is not an MSDS. According to EU and US regulations we are not required to supply an MSDS for a product, which is not classified as hazardous.

Version# 1 Revision Date: N.A. Page 1 of 1 Printed:12/23/2005 Created Date:070104 QC Form 1528

SAFETY STATEMENT This document is only available in English. 1. Identification of the substance/preparation and company/undertaking

Product/Kit Name: RETROscript® Catalog #: 1710 Product/Component Name: Control PCR Primers

Catalog Number: 5741G

Supplier: Ambion, Inc.

Emergency Telephone Numbers:

USA: 1-800-888-8804

Europe: +44 (0) 1480 373 020

2. Composition/ information on ingredients Substance/Preparation: To the present knowledge of Ambion, Inc., this product does not contain any hazardous ingredients in quantities requiring reporting in this section, in accordance with EU regulations or National regulations.

To Ambion’s knowledge this substance/preparation is not classified as hazardous according to EU, US, or any other known national regulations.

9. Physical and chemical properties

Physical State: Liquid Color: Colorless 16. Other information:

To the best of Ambion’s knowledge, the information contained herein is accurate. However, Ambion, Inc. does not assume any liability whatsoever for the accuracy or completeness of the information contained herein. Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used with caution.

This is not an MSDS. According to EU and US regulations we are not required to supply an MSDS for a product, which is not classified as hazardous.

Version# 1 Revision Date: N.A. Page 1 of 1 Printed:12/23/2005 Created Date:070104 QC Form 1763

SAFETY STATEMENT This document is only available in English. 1. Identification of the substance/preparation and company/undertaking

Product/Kit Name: RETROscript® Catalog #: 1710 Product/Component Name: Control Template RNA

Catalog Number: 7131G

Supplier: Ambion, Inc.

Emergency Telephone Numbers:

USA: 1-800-888-8804

Europe: +44 (0) 1480 373 020

2. Composition/ information on ingredients Substance/Preparation: To the present knowledge of Ambion, Inc., this product does not contain any hazardous ingredients in quantities requiring reporting in this section, in accordance with EU regulations or National regulations.

To Ambion’s knowledge this substance/preparation is not classified as hazardous according to EU, US, or any other known national regulations.

9. Physical and chemical properties

Physical State: Liquid Color: Colorless 16. Other information:

To the best of Ambion’s knowledge, the information contained herein is accurate. However, Ambion, Inc. does not assume any liability whatsoever for the accuracy or completeness of the information contained herein. Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used with caution.

This is not an MSDS. According to EU and US regulations we are not required to supply an MSDS for a product, which is not classified as hazardous.

Version# 1 Revision Date: N.A. Page 1 of 1 Printed:12/23/2005 Created Date:070104 QC Form 1529

SAFETY STATEMENT This document is only available in English. 1. Identification of the substance/preparation and company/undertaking

Product/Kit Name: RETROscript® Catalog #: 1710 Product/Component Name: dNTP Mix

Catalog Number: 8228G

Supplier: Ambion, Inc.

Emergency Telephone Numbers:

USA: 1-800-888-8804

Europe: +44 (0) 1480 373 020

2. Composition/ information on ingredients Substance/Preparation: To the present knowledge of Ambion, Inc., this product does not contain any hazardous ingredients in quantities requiring reporting in this section, in accordance with EU regulations or National regulations.

To Ambion’s knowledge this substance/preparation is not classified as hazardous according to EU, US, or any other known national regulations.

9. Physical and chemical properties

Physical State: Liquid Color: Colorless 16. Other information:

To the best of Ambion’s knowledge, the information contained herein is accurate. However, Ambion, Inc. does not assume any liability whatsoever for the accuracy or completeness of the information contained herein. Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used with caution.

This is not an MSDS. According to EU and US regulations we are not required to supply an MSDS for a product, which is not classified as hazardous.

AMBION, INC. RETROscript® Catalog #1710 HIGH-RES Gel Loading Buffer Material Safety Data Sheet

Version 1 Page 1 of 3 Printed: 12/23/2005 Created:070104 QC Form: 1530 Revision Date: N.A

Section 1-Identification of Product 8542G1 WHMIS Classification

Health: 3 Fire: 0 Stability: 0

Contact Information Ambion, Inc 2130 Woodward St. Austin, TX 78744-1832 Tel: +1 512 651 0200 US Toll-free Tel: 800 888 8804 E-mail: [email protected] Web address www.ambion.com Ambion (Europe) LTD Huntingdon, Cambidgeshire UK PE29 6XY Tel:+44 (0)1480 373 020 Fax: +44 (0)1480 373 010 E-mail: [email protected] Web address: www.ambion.com Emergency Contact: In Europe, call 112. In USA, call 911 Section 2-Composition/Information Hazardous Ingredients (Specific) % CAS # LD50 LC50 Trade secret ~32 Trade secret N.A.

N.A

Trade secret ~33 Trade secret Oral-Rat LD50: 12,600

mg/kg

N.A.

Section 3-Hazard Identification Routes of Entry

Skin Contact Causes irritation, Harmful Skin Absorption Causes irritation, Harmful Eye Contact Causes irritation Inhalation Causes irritation Ingestion TOXIC

[Emergency Overview] N.A. WHMIS Symbols N.A. [Potential Health Effects] -Poison by ingestion, intraperitoneal, and subcutaneous routes. When heated to decomposition it emits very toxic fumes of NOx, NH3, and Cl¯. Poison by subcutaneous route. Mildly toxic by ingestion. Human systemic effects by ingestion: headache and nausea or vomiting. Experimental reproductive effects. Human mutation data reported. A skin and eye irritant. In the form of mist it is a nuisance particulate and inhalation irritant. Combustible liquid when exposed to heat, flame, or powerful oxidizers. Mixtures with hydrogen peroxide are highly explosive. Ignites on contact with potassium permanganate, calcium hypochlorite. Mixture with nitric acid + sulfuric acid forms the explosive glyceryl nitrate. Mixture with perchloric acid + lead oxide forms explosive perchlorate esters. Confined mixture with chlorine explodes if heated to 70–80º. Can react violently with acetic anhydride, aniline + nitrobenzene, Ca(OCl)2, CrO3, Cr2O3, F2 + PbO, phosphorus triiodide, ethylene oxide + heat, KMnO4, K2O2, AgClO4, Na2O2, NaH. Energetic reaction with sodium hydride. Mixture with nitric acid + hydrofluoric acid is a storage hazard due to gas evolution. To fight fire, use alcohol foam, CO2, dry chemical. When heated to decomposition it emits acrid smoke and fumes. Section 4-First Aid Measures Skin Contact Get medical aid. Flush skin with plenty of water for at least 15 minutes while removing

contaminated clothing and shoes Eye Contact Flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower

eyelids. Get medical aid immediately Inhalation Get medical aid immediately. Remove from exposure and move to fresh air immediately. If not

Breathing, give artificial respiration. If breathing is difficult, give oxygen. Ingestion Never give anything by mouth to an unconscious person. Do NOT induce vomiting. If conscious

and alert, rinse mouth and drink 2-4 cupfuls of milk or water. Get medical aid if irritation or symptoms occur.

Section 5-Fire Fighting Measures Flammable Non-flammable Means of Extinction Use water spray to cool fire-exposed containers. Use agent

most appropriate to extinguish fire. Use water spray, dry chemical, carbon dioxide, or appropriate foam

Flashpoint (oC) and Method Not Tested

AMBION, INC. RETROscript® Catalog #1710 HIGH-RES Gel Loading Buffer Material Safety Data Sheet

Version 1 Page 2 of 3 Printed: 12/23/2005 Created:070104 QC Form: 1530 Revision Date: N.A

Upper Flammable Limit (% by volume) Not Tested Lower Flammable Limit (% by volume) Not Tested Autoignition Temperature (oC) Not Tested Explosion Data – Sensitivity to Impact Not Tested Explosion Data – Sensitivity to Static Discharge Not Tested Hazardous Combustion Products Not Tested NFPA Health: 3 Fire: 0 Stability: 0 Section 6-Accidental Release Measures Leak and Spill Procedures Sweep up, place in a bag and hold for waste disposal. Avoid raising dust.

Ventilate area and wash spill site after material pickup is complete. Section 7-Handling and Storage Handling Procedures and Equipment

Wash thoroughly after handling. Wash hands before eating. Use with adequate ventilation. Avoid contact with eyes, skin, and clothing. Keep container tightly closed. Avoid ingestion and inhalation. Wash clothing before reuse.

Storage Requirements Keep in a tightly closed container, stored in a cool, dry, ventilated area. Protect against physical damage. Isolate from incompatible substances. Containers of this material may be hazardous when empty since they retain product residues (dust, solids); observe all warnings and precautions listed for the product.

Section 8-Exposure Control/Personal Protection Exposure Limits ACGIH TLV 10 mg/m3 TWA OSHA PEL 15 mg/m3 TWA (total dust); 5 mg/m3 TWA (respirable fraction) Other (specify) N.A. Engineering Controls (specific)

General Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower. Use adequate ventilation to keep airborne concentrations low.

Local Exhaust A system of local and/or general exhaust is recommended to keep employee exposures as low as possible. Local exhaust ventilation is generally preferred because it can control the emissions of the contaminant at its source, preventing dispersion of it into the general work area. Please refer to the ACGIH document, Industrial Ventilation, A Manual of Recommended Practices, most recent edition, for details.

Other N.A. Personal Protective Equipment (specific)

Gloves Synthetic Gloves Respirator A respiratory protection program that meets OSHA's 29 CFR 1910.134 and

ANSI Z88.2 requirements or European Standard EN 149 must be followed whenever workplace conditions warrant a respirator's use.

Eye Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166. Maintain eye wash fountain and quick-drench facilities in work area.

Footwear Closed toe shoes Clothing Wear appropriate protective clothing to prevent skin exposure. Other N.A.

Section 9-Physical and Chemical Properties Physical State Liquid Odor and Appearance Odorless / Clear Odor Threshold (ppm) Not Tested Specific Gravity Not Tested Vapor Density (Air=1) Not Tested Vapor Pressure (mmHg) Not Tested Evaporation Rate Not Tested Boiling Point (oC) Not Tested Freezing Point (oC) Not Tested pH Not Tested Coefficient of Water/Oil Distribution Not Tested [Solubility in Water] Not Tested

AMBION, INC. RETROscript® Catalog #1710 HIGH-RES Gel Loading Buffer Material Safety Data Sheet

Version 1 Page 3 of 3 Printed: 12/23/2005 Created:070104 QC Form: 1530 Revision Date: N.A

Section 10-Stability and Reactivity Chemical Stability Stable Incompatible with other substances Strong oxidizing agents. Glycerol-Acetic anhydride, potassium

permanganate, strong acids, caustics (e.g. ammonia, ammonium hydroxide, calcium hydroxide, potassium hydroxide, sodium hydroxide), isocyanates, oxidizing agents, aliphatic amines

Reactivity N.A. Hazardous Decomposition Products Hazardous Decomposition Products: Carbon monoxide, Carbon

dioxide, Nitrogen oxides, Hydrogen chloride gas. Glycerol- Carbon monoxide, irritating and toxic fumes and gases, carbon dioxide.

Section 11-Toxicological Information Acute Effects Not Tested Chronic Effects Not Tested Irritancy of Product Not Tested Skin Sensitization Not Tested Respiratory Sensitization Not Tested

Carcinogenicity IARC (1,2A, or 2B) Not Listed ACGIH (A1, A2, or A3) Not Listed Reproductive Toxicity Not Tested Teratogenicity Not Tested Embryotoxicity Not Tested Mutagenicity Not Tested Name of Synergistic Products/Effects

N.A.

Section 12-Ecological Information Aquatic Toxicity Not Tested

Section 13-Disposal Considerations Waste Disposal Chemical waste generators must determine whether a discarded chemical is

classified as a hazardous waste. US EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3. Additionally, waste generators must consult state and local hazardous waste regulations to ensure complete and accurate classification.

Section 14-Transport Information Special Shipping Information

PIN Not Listed TDG Not Listed [DOT] Proper Shipping Name: Toxic, liquids, organic, n.o.s.

UN#: 2810 Class: 6.1 Packing Group: Packing Group III Hazard Label: Keep away from food PIH: Not PIH

[IMO] Not Listed [ICAO] Not Listed

Section 15-Regulatory Information [WHMIS Classification] Not Listed [OSHA] Not Listed [SARA] Not Listed - CAS # Trade secret: chronic [TSCA] Trade secret - Listed

Section 16-Other Information This bulletin is for your guidance and is based upon information and tests believed to be reliable. Ambion makes no guarantee of the accuracy or completeness of the data and shall not be liable for any damages thereto. The data are offered solely for your consideration, investigation, and verification. These suggestions should not be confused with state, municipal, or insurance requirements, or with national safety codes and constitute no warranty. Any use of these data and information must be determined by the user to be in accordance with applicable federal, state, and local regulations.

AMBION, INC. RETROscript® Catalog #1710 10X PCR Buffer Material Safety Data Sheet

Version 1 Page 1 of 3 Printed: 12/23/2005 Created:070104 QC Form: 1524 Revision Date: N.A

Section 1-Identification of Product 8701G WHMIS Classification

Health: 2 Fire: 1 Stability: 1

Contact Information Ambion, Inc 2130 Woodward St. Austin, TX 78744-1832 Tel: +1 512 651 0200 US Toll-free Tel: 800 888 8804 E-mail: [email protected] Web address www.ambion.com Ambion (Europe) LTD Huntingdon, Cambidgeshire UK PE29 6XY Tel:+44 (0)1480 373 020 Fax: +44 (0)1480 373 010 E-mail: [email protected] Web address: www.ambion.com Emergency Contact: In Europe, call 112. In USA, call 911 Section 2-Composition/Information Hazardous Ingredients (Specific) % CAS # LD50 LC50 Trade secret

1-30

Trade secret

Oral-Rat LD50:2600

mg/kg

N.A.

Trade secret 1-30 Trade secret Oral-rat 2800 mg/kg

N.A.

Section 3-Hazard Identification Routes of Entry

Skin Contact Causes irritation to skin. Symptoms include redness, itching, and pain Skin Absorption Causes irritation Eye Contact Causes irritation, redness, and pain. Inhalation Causes irritation to the respiratory tract. Symptoms may include

coughing, shortness of breath. Can cause nausea, headache and vomiting. Exposure may result in blood in urine, difficulty breathing, irregular heartbeat, anemia, weakness, drunkenness, bluish skin color, lung congestion, kidney damage, paralysis, convulsions unconsciousness and coma

Ingestion Large quantities can produce gastrointestinal irritation and vomiting. May produce weakness and circulatory problems. May affect heart. In severe cases, ingestion may be fatal. Tris- Exposure can cause nausea, headache, vomiting, diarrhea, weakness, drunkenness, restlessness, bluish skin color, paralysis and coma

[Emergency Overview] N.A. WHMIS Symbols N.A. [Potential Health Effects] A human poison by ingestion. Poison experimentally by ingestion, intravenous, and intraperitoneal routes. Human systemic effects by ingestion: nausea, blood clotting changes, and cardiac arrhythmias. An eye irritant. Mutation data reported. Explosive reaction with BrF3; sulfuric acid + potassium permanganate. When heated to decomposition it emits toxic fumes of K2O and Cl¯. Moderately toxic by ingestion and intravenous routes. When heated to decomposition it emits toxic fumes of NOx Section 4-First Aid Measures Skin Contact Get medical aid. Flush skin with plenty of water for at least 15 minutes while removing

contaminated clothing and shoes Eye Contact Flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower

eyelids. Get medical aid immediately. Inhalation Get medical aid immediately. Remove from exposure and move to fresh air immediately. If not

Breathing, give artificial respiration. If breathing is difficult, give oxygen. Ingestion Induce vomiting immediately as directed by medical personnel. Never give anything by mouth

to an unconscious person. Call a physician. Section 5-Fire Fighting Measures Flammable Slightly flammable Means of Extinction CO2, H2O, Foam, Dry Chemical Flashpoint (oC) and Method Not Tested Upper Flammable Limit (% by volume) Not Tested

AMBION, INC. RETROscript® Catalog #1710 10X PCR Buffer Material Safety Data Sheet

Version 1 Page 2 of 3 Printed: 12/23/2005 Created:070104 QC Form: 1524 Revision Date: N.A

Lower Flammable Limit (% by volume) Not Tested Autoignition Temperature (oC) Not Tested Explosion Data – Sensitivity to Impact Not Tested Explosion Data – Sensitivity to Static Discharge Not Tested Hazardous Combustion Products N.A. NFPA Health: 2 Fire: 1 Stability: 1 Section 6-Accidental Release Measures Leak and Spill Procedures Sweep up, place in a bag and hold for waste disposal. Avoid raising dust. Ventilate area and wash spill site after material pickup is complete. Section 7-Handling and Storage Handling Procedures and Equipment

Wash thoroughly after handling. Wash hands before eating. Use with adequate ventilation. Avoid contact with eyes, skin, and clothing. Keep container tightly closed. Avoid ingestion and inhalation. Wash clothing before reuse.

Storage Requirements Keep in a tightly closed container, stored in a cool, dry, ventilated area. Protect against physical damage. Isolate from incompatible substances. Containers of this material may be hazardous when empty since they retain product residues (dust, solids); observe all warnings and precautions listed for the product.

Section 8-Exposure Control/Personal Protection Exposure Limits ACGIH TLV Not Tested OSHA PEL Not Tested Other (specify) Not Tested Engineering Controls (specific)

General Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower. Use adequate ventilation to keep airborne concentrations low.

Local Exhaust A system of local and/or general exhaust is recommended to keep employee exposures as low as possible. Local exhaust ventilation is generally preferred because it can control the emissions of the contaminant at its source, preventing dispersion of it into the general work area. Please refer to the ACGIH document, Industrial Ventilation, A Manual of Recommended Practices, most recent edition, for details.

Other N.A. Personal Protective Equipment (specific)

Gloves Synthetic gloves Respirator A respiratory protection program that meets OSHA's 29 CFR 1910.134 and

ANSI Z88.2 requirements or European Standard EN 149 must be followed whenever workplace conditions warrant a respirator's use.

Eye Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166. Maintain eye wash fountain and quick-drench facilities in work area.

Footwear Closed toe shoes Clothing Wear appropriate protective clothing to prevent skin exposure. Other N.A.

Section 9-Physical and Chemical Properties Physical State Liquid Odor and Appearance Odorless / Colorless Odor Threshold (ppm) Not Tested Specific Gravity Not Tested Vapor Density (Air=1) Not Tested Vapor Pressure (mmHg) Not Tested Evaporation Rate Not Tested Boiling Point (oC) Not Tested Freezing Point (oC) Not Tested pH 8.3 Coefficient of Water/Oil Distribution Not Tested [Solubility in Water] Not Tested

AMBION, INC. RETROscript® Catalog #1710 10X PCR Buffer Material Safety Data Sheet

Version 1 Page 3 of 3 Printed: 12/23/2005 Created:070104 QC Form: 1524 Revision Date: N.A

Section 10-Stability and Reactivity Chemical Stability Stable Incompatible with other substances Bromine trifluoride; potassium permanganate plus sulfuric acid.

Copper, brass, aluminum, and oxidizing agents Reactivity N.A. Hazardous Decomposition Products Oxides of the contained metal and halogen, possibly also free, or

ionic halogen. Burning may produce carbon monoxide, carbon dioxide, and nitrogen oxides. When heated to temperatures above 300C (572F) it emits toxic fumes of chlorine gas.

Section 11-Toxicological Information Acute Effects Not Tested Chronic Effects Not Tested Irritancy of Product Not Tested Skin Sensitization Not Tested Respiratory Sensitization Not Tested

Carcinogenicity IARC (1,2A, or 2B) Not Listed ACGIH (A1, A2, or A3) Not Listed Reproductive Toxicity Not Listed Teratogenicity Not Listed Embryotoxicity Not Listed Mutagenicity Not Listed Name of Synergistic Products/Effects

Not Listed

Section 12-Ecological Information Aquatic Toxicity No Data

Section 13-Disposal Considerations Waste Disposal Chemical waste generators must determine whether a discarded chemical is

classified as a hazardous waste. US EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3. Additionally, waste generators must consult state and local hazardous waste regulations to ensure complete and accurate classification.

Section 14-Transport Information Special Shipping Information

PIN Not Listed TDG Not Listed [DOT] Not Listed [IMO] Not Listed [ICAO] Not Listed

Section 15-Regulatory Information [WHMIS Classification] Not Listed [OSHA] Not Listed [SARA] 302-no 313-no 311/312 Acute: yes Chronic: no Trade secret- 311/312:

Acute [TSCA] Yes

Section 16-Other Information This bulletin is for your guidance and is based upon information and tests believed to be reliable. Ambion makes no guarantee of the accuracy or completeness of the data and shall not be liable for any damages thereto. The data are offered solely for your consideration, investigation, and verification. These suggestions should not be confused with state, municipal, or insurance requirements, or with national safety codes and constitute no warranty. Any use of these data and information must be determined by the user to be in accordance with applicable federal, state, and local regulations.

AMBION, INC. RETROscript® Catalog #1710 10X RT Buffer Material Safety Data Sheet

Version 1 Page 1 of 3 Printed: 12/23/2005 Created:070104 QC Form: 1525 Revision Date: N.A

Section 1-Identification of Product 8703G WHMIS Classification

Health: 2 Fire: 1 Stability: 1

Contact Information Ambion, Inc 2130 Woodward St. Austin, TX 78744-1832 Tel: +1 512 651 0200 US Toll-free Tel: 800 888 8804 E-mail: [email protected] Web address www.ambion.com Ambion (Europe) LTD Huntingdon, Cambidgeshire UK PE29 6XY Tel:+44 (0)1480 373 020 Fax: +44 (0)1480 373 010 E-mail: [email protected] Web address: www.ambion.com Emergency Contact: In Europe, call 112. In USA, call 911 Section 2-Composition/Information Hazardous Ingredients (Specific) % CAS # LD50 LC50 Trade secret 5-35 Trade secret Oral-Rat

LD50: 2600 mg/kg

No information

Trade secret 5-35 Trade secret Oral-Rat LD50: 5900

mg/kg

No information

Section 3-Hazard Identification Routes of Entry

Skin Contact Causes skin irritation Skin Absorption Causes skin irritation Eye Contact Causes eye irritation Inhalation Causes respiratory irritation Ingestion

Large quantities can produce gastrointestinal irritation and vomiting. May produce weakness and circulatory problems. May affect heart. In severe cases, ingestion may be fatal.

[Emergency Overview] N.A. WHMIS Symbols N.A. [Potential Health Effects] A human poison by ingestion. Poison experimentally by ingestion, intravenous, and intraperitoneal routes. Human systemic effects by ingestion: nausea, blood clotting changes, cardiac arrhythmias. An eye irritant. Mutation data reported. Explosive reaction with BrF3; sulfuric acid + potassium permanganate. When heated to decomposition it emits toxic fumes of K2O and Cl¯. Moderately toxic by ingestion and intravenous routes. When heated to decomposition it emits toxic fumes of NOx Skin Contact Get medical aid. Flush skin with plenty of water for at least 15 minutes while removing

contaminated clothing and shoes Eye Contact Flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower

eyelids. Get medical aid immediately Inhalation Get medical aid immediately. Remove from exposure and move to fresh air immediately. If not

breathing, give artificial respiration. If breathing is difficult, give oxygen. Ingestion Induce vomiting immediately as directed by medical personnel. Never give anything by mouth

to an unconscious person. Call a physician. Section 5-Fire Fighting Measures Flammable Slightly flammable Means of Extinction CO2, H2O, Foam, Dry Chemical Flashpoint (oC) and Method Not Tested Upper Flammable Limit (% by volume) Not Tested Lower Flammable Limit (% by volume) Not Tested Autoignition Temperature (oC) Not Tested Explosion Data – Sensitivity to Impact Not Tested Explosion Data – Sensitivity to Static Discharge Not Tested

AMBION, INC. RETROscript® Catalog #1710 10X RT Buffer Material Safety Data Sheet

Version 1 Page 2 of 3 Printed: 12/23/2005 Created:070104 QC Form: 1525 Revision Date: N.A

Hazardous Combustion Products When heated to decomposition it emits corrosive hydrochloric acid vapor. When heated to temperatures above 300C (572F) it emits toxic fumes of chlorine gas.

NFPA Health: 2 Fire: 1 Stability: 1 Section 6-Accidental Release Measures Leak and Spill Procedures Sweep up, place in a bag and hold for waste disposal. Avoid raising dust. Ventilate area and wash spill site after material pickup is complete. Section 7-Handling and Storage Handling Procedures and Equipment

Avoid inhalation. Avoid contact with eyes, skin, and clothing. Avoid prolonged or repeated exposure

Storage Requirements Keep in a tightly closed container, stored in a cool, dry, ventilated area. Protect against physical damage. Isolate from incompatible substances. Containers of this material may be hazardous when empty since they retain product residues (dust, solids); observe all warnings and precautions listed for the product.

Section 8-Exposure Control/Personal Protection Exposure Limits ACGIH TLV Not Listed OSHA PEL Not Listed Other (specify) N.A. Engineering Controls (specific)

General Use adequate ventilation to keep airborne concentrations low. Local Exhaust A system of local and/or general exhaust is recommended to keep employee

exposures as low as possible. Local exhaust ventilation is generally preferred because it can control the emissions of the contaminant at its source, preventing dispersion of it into the general work area. Please refer to the ACGIH document, Industrial Ventilation, A Manual of Recommended Practices, most recent edition, for details.

Other N.A. Personal Protective Equipment (specific)

Gloves Synthetic Gloves Respirator A respiratory protection program that meets OSHA's 29 CFR 1910.134 and

ANSI Z88.2 requirements or European Standard EN 149 must be followed whenever workplace conditions warrant a respirator's use.

Eye Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166. Maintain eye wash fountain and quick-drench facilities in work area

Footwear Closed toe shoes Clothing Wear appropriate protective clothing to prevent skin exposure Other

Section 9-Physical and Chemical Properties Physical State Liquid Odor and Appearance Odorless / Colorless Odor Threshold (ppm) Not Tested Specific Gravity Not Tested Vapor Density (Air=1) Not Tested Vapor Pressure (mmHg) Not Tested Evaporation Rate Not Tested Boiling Point (oC) Not Tested Freezing Point (oC) Not Tested pH 8.3 Coefficient of Water/Oil Distribution Not Tested [Solubility in Water] Not Tested

Section 10-Stability and Reactivity Chemical Stability Stable Incompatible with other substances

Bromine trifluoride; potassium permanganate plus sulfuric acid. Copper, brass, aluminum, and oxidizing agents

Reactivity Not Tested

AMBION, INC. RETROscript® Catalog #1710 10X RT Buffer Material Safety Data Sheet

Version 1 Page 3 of 3 Printed: 12/23/2005 Created:070104 QC Form: 1525 Revision Date: N.A

Hazardous Decomposition Products Oxides of the contained metal and halogen, possibly also free, or ionic halogen. Burning may produce carbon monoxide, carbon dioxide, and Nitrogen oxides. When heated to temperatures above 300C (572F) it emits toxic fumes of chlorine gas

Section 11-Toxicological Information Acute Effects Not Tested Chronic Effects Not Tested Irritancy of Product Not Tested Skin Sensitization Not Tested Respiratory Sensitization Not Tested

Carcinogenicity IARC (1,2A, or 2B) Not Listed ACGIH (A1, A2, or A3) Not Listed Reproductive Toxicity Not Listed Teratogenicity Not Listed Embryotoxicity Not Listed Mutagenicity Not Listed Name of Synergistic Products/Effects

Not Listed

Section 12-Ecological Information Aquatic Toxicity No Data

Section 13-Disposal Considerations Waste Disposal Chemical waste generators must determine whether a discarded chemical is

classified as a hazardous waste. US EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3. Additionally, waste generators must consult state and local hazardous waste regulations to ensure complete and accurate classification.

Section 14-Transport Information Special Shipping Information

PIN Not Listed TDG Not Listed [DOT] Not Listed [IMO] Not Listed [ICAO] Not Listed

Section 15-Regulatory Information [WHMIS Classification] Not Listed [OSHA] Not Listed [SARA] 302-no 313-no 311/312 Acute: yes Chronic: no Trade secret- 311/312: Acute [TSCA] Yes

Section 16-Other Information This bulletin is for your guidance and is based upon information and tests believed to be reliable. Ambion makes no guarantee of the accuracy or completeness of the data and shall not be liable for any damages thereto. The data are offered solely for your consideration, investigation, and verification. These suggestions should not be confused with state, municipal, or insurance requirements, or with national safety codes and constitute no warranty. Any use of these data and information must be determined by the user to be in accordance with applicable federal, state, and local regulations.

Version# 1 Revision Date: N.A. Page 1 of 1 Printed:12/23/2005 Created Date:070104 QC Form 1764

SAFETY STATEMENT This document is only available in English. 1. Identification of the substance/preparation and company/undertaking

Product/Kit Name: RETROscript® Catalog #: 1710 Product/Component Name: Nuclease-free Water

Catalog Number: 9914G

Supplier: Ambion, Inc.

Emergency Telephone Numbers:

USA: 1-800-888-8804

Europe: +44 (0) 1480 373 020

2. Composition/ information on ingredients Substance/Preparation: To the present knowledge of Ambion, Inc., this product does not contain any hazardous ingredients in quantities requiring reporting in this section, in accordance with EU regulations or National regulations.

To Ambion’s knowledge this substance/preparation is not classified as hazardous according to EU, US, or any other known national regulations.

9. Physical and chemical properties

Physical State: Liquid Color: Colorless 16. Other information:

To the best of Ambion’s knowledge, the information contained herein is accurate. However, Ambion, Inc. does not assume any liability whatsoever for the accuracy or completeness of the information contained herein. Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used with caution.

This is not an MSDS. According to EU and US regulations we are not required to supply an MSDS for a product, which is not classified as hazardous.


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