Analysis of lytic proteins pinholin and Rz/Rz1
Sue Parks and Lawangin Khan
2
S R Rz
Rz1
Lytic Cassette
3
Comparative analysis of Trans-Membrane domain Motifs in pinholin proteins
Two Domains in the Pinholin Protein
Pang et al.(2010) Mutational Analysis of S21 pinholin. Molecular Biology, 76(1): 66-77.
Figure 1D: Phage S21 reading frame
Traditional Holin Model
TM1
TM2
TM3
TMD2TMD1
Text
Motifs TMD1 TMD2MXXM
Comparative analysis of lysis protein, Rz1 cleavage
sitesLawangin Khan
Localization? Why Cleave?
Cleave site
Cleavage site conserved? Determining cleavage site
• Determined by LipoP, (www.cbs.dtu.dk/services/LipoP/)
Comparative Analysis
Relative Consensus and Position
• Krupovic’s lipobox consensus: L(A/S)(G/A)C