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AP Lab Skills Guide
Tube 1 (substrate) Tube 2 (enzyme)H2O2
GuaiacolDistilled H2O
Peroxidase Distilled water
+ Mix contentsBegin Timing
Tube 3(tubes 1 + 2)Begin Timing
AP Lab Skills Guide
Tube 3(tubes 1 + 2)Begin Timing
Each minute for 5 min compare the color of tube 3 to the standard
Time (min) Color
1 1
2 2
3 4…Graph the data…
AP Lab Skills Guide
Each minute for 5 min compare the color of tube 3 to the standard
Time (min) Color
1 1
2 2
3 2
4 3
5 5
6 6
7 8
8 10
You are determining how long to let the reaction go to get a value of “5” as your baseline for comparisons in the next step…you will see…we will run all future reactions for 5 minutes and look at color and compare to enzyme in distilled water….
AP Lab Skills Guide
Tube 1 Tube 2 Tube 3 Tube 4 Tube 5 Tube 6PeroxidaseH2O2
pH 2 buffer
PeroxidaseH2O2
pH 4 buffer
PeroxidaseH2O2
pH 6 buffer
PeroxidaseH2O2
pH 8 buffer
PeroxidaseH2O2
pH 10 buffer
PeroxidaseH2O2
pH 12 buffer
Cover with parafilm, gently mix and measure color at 0 minutes and color change at time determined in part 1 (5 minutes)...
AP Lab Skills Guide
pH Color
2 1
4 2
6 5
8 3
10 1
12 1
Of course, we should combine the class data, take averages, SD, SE and graph with error bars…