Application of Metagenomics Data Pool
in Structured Problem Solving
Kalliopi Chalkou
Group Food Safety / SQ Manager
Zoltan Syposs
Group QSE Director
A Bottled Water Industry Example
Our portfolio requires a more sensitive approach to
Food Safety
Only Natural ingredientsNo preservatives
2000
Carbonated Sensitive BeveragesNo Carbonation
Preservatives Artificial ingredients
Natural ingredients No preservatives
2010 2020
80%90% 60%
We started receiving repeated and sporadic consumer
complaints for off-note in one of our NMW
Off -note characterized as
Sulfuric
…never been able to detect
during production
Bottled Water Food Safety Incidents 2015 – 2018
Total incidents EU incidents
Thousands of Samples
indicated full compliance
…or not?
Collaboration & Partnerships with Universities
Some of our Partnerships on:
❖ Methodology development
❖ Structured problem solving
❖ Youth development/Education
❖ Research topics
❖ Trainee programs
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Fixed vs.
Growth
Mindset
Go beyond the Extra
Mile…
Scrutinize the facts
..The need to see the “small” picture
Changing our approach
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• Samples from all process steps + off-note complaints
• Community DNA extraction
• Next Generation sequencing (NGS) – High Throughput
• Phylogenetic assignment (OTUs), estimation of α, β diversity
Wells
Collector
Sand Filter
Sporadic off note
Microbiological sampling pointSensory analysis sampling point
Buffer Tanks
FillerFilter units
We mapped the process again…
We built a new NMW specific database
Wells Buffer/ Production tanks
Biofilm indication in the tanks:
Species with presence < 1% accumulate and overgrow in the
buffer and production tanks
Biodiversity Decrease / Species dominance increase
Abundant species in samples with off note
Abundant species in off note samples low presence in previous process steps
Species detected in off note samples
Perlucidibaca sp and Polaromonas sp - Reducing nitrate and produce indole, heterocyclic organic compound
Limnobacter sp - Representatives that exceeded 70% samples are heterotrophic, aerobic thiosulfate oxidizing
Cytophagaceae - Commonly isolated from marine, freshwater, and terrestrial habitats
Bdellovibrio sp - Obligate aerobic bacteria that are considered bacterial predators and parasites. Biofilm colonizers
Methylotenera sp - Obligately methylamine-utilizing species aerobic, Gram-negative
Wells Finished Product
In presence of carbon from organic compounds, bacteria metabolize Selenium to dymethylediselenide (DMDS), dymethyleselenide (DMS) and similar compounds which could be detected in very low concentration by
The above mechanism is not valid
Root Cause Analysis
For PET
For RGB
❖ Apply Biofilm build up Prevention in filling Equipment
❖Try again…until the Root Cause fully understood
❖ Build structured data
❖ Listen to Data
❖ “Scrutinize the Facts”
Lessons Learned
Bottled Water Quality/Food Safety Incidents 2015 – 2018
Thank you
No off-note complaints in the past >12 months
Thank You!