Arbutus, Catlin, Green, Oneida, Onondaga, and Wolf.
Presented by
Thomas Clement
&
Amanda Stella
http://www.imagequest3d.com/photos/phytoplankton/s003.jpg
http://www.nies.go.jp/biology/mcc/images/PCD5008/0998L.jpg
Why are phytoplankton important?
• Produce O2 in lakes
• Indicator of primary production
• Control Other populations of organisms
http://www.umanitoba.ca/institutes/fisheries/227_305.jpg
How phytoplankton is measured
• Sampling Techniques– Van Dorn – Preservation with Lugols solution
• Lab Techniques– Water settled for several days– Increased concentration– Identified to Genus
http://www.fisheries.org/education/fisheries_techniques/Chapter4/Removal%20of%20a%20water%20sampler%20from%20a%20Van%20Dorn%20sampler.jpg
Chlorophyll
• Main pigment in photosynthetic organisms
• Commonly used as an indicator of production
http://www.public.asu.edu/~jpbirk/3dstruct/chlorophyll.gif
Chlorophyll Analysis
• Water sampled out in field
• In Lab– Mix sample– Measure sample – Filtered– Packaged in foil– Frozen until analysis
http://www.umass.edu/tei/mwwp/Images/Filt.gif&imgrefurl=http://www.umass.edu/tei/mwwp/chloro.html&h=267&w=247&sz=6&hl=en&start=71&tbnid=DZS0KGvprZR8wM:&tbnh=113&tbnw=105&prev=/images%3Fq%3Dchlorophyll%26start%3D60%26ndsp%3D20%26svnum%3D10%26hl%3Den%26lr%3D%26safe%3Doff%26client%3Dfirefox-%26rls%3Dorg.mozilla:en-US:official%26sa%3DN
Chlorophyll Analysis Con’t
• 90% buffered acetone used for extraction
• Filters added to 15ml alkaline acetone solution
• Refrigerated for 24 hours• Fluorometer was used
– Fluorometer produces a concentration in µgrams/liter
http://cacique.uprm.edu/gers/fluorometer.jpg
Chlorophyll concentrations in the lakes
0
5
10
15
20
25
0 20 40 60 80 100 120 140 160
Chl a (micrograms/liter)
De
pth
(m
)
Onondaga
Catlin
Oneida
Green
Wolf
Arbutus
Phytoplankton Arbutus
012345678
0 2 4 6 8
# of Individuals
Dep
th
Stynura
Mallamonas
Microcystis
Dinobryon
Chlamydomon
Catlin
0
2
4
6
8
10
12
14
0 2 4 6 8 10
# of Individuals
Dep
th
Chrysophyte
Centric Diatom
Flagellate
Tabellaria
Mougeotia
Green
0
5
10
15
20
0 0.5 1 1.5 2 2.5
# of Individuals
Dep
th
Euglenophyta
Oneida
0
2
4
6
8
10
12
0 20 40 60 80 100
# of Individuals
Dep
ths
Aulacosira
Microcystis
Small Flagellates
Mallanomous
Acinastrum
Phytoplankton Con’tOnondaga
02468
1012141618
0 10 20 30 40 50
Depth
# o
f In
div
idu
als
Microcystis
Chrysophyaceae
Actinastrum
Polyblepharides
Bacillariophyceae
Arbutus
012345678
0 2 4 6 8
# of Individuals
Dep
th
Stynura
Mallamonas
Microcystis
Dinobryon
Chlamydomon
Phytoplankton AbundanceArbutus
Catlin
Green
Phytoplankton Abundance Cont’d
OneidaOnondaga
Conclusions
• Cross-Lake Comparisons– Wide variety of phytoplankton
• Sampling Error– Sampling day conditions– Analyzing techniques