10 TiJS. No.

VWdillfav, "Wia. Tina

AGRICULTURAL SCIENTISTS RECRUITMENT BOARD

71:Ow/Roll No.

iTruErn/Medium

t.nigit-Treithreffiki/AliEw= Signature of Invigilator/Supervisor

1 0

wri *IS. No.

A.S.R.B. 2009

Vtf qui4ch 94 guar(

AGRICULTURAL SCIENTISTS RECRUITMENT BOARD

IFT41 II ( ctucitiAch ) PAPER II (Professional)

fttiii: T10.11401 Pila SUBJECT : Agricultural Biotechnology

giTer .22 10- : 200

Time Allowed : 2-1 Hours

Max. Marks : 200

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certificate issued to the candidate.

2. Instructions given in the admission certificate should be followed strictly.

a Attempt any 20 questions.

4. Answers to the question should be brief and to the point (not more than one page of the answer book).

[To trtz titseit/Total pages 4]

Sill Agri/09-10—PN

3 10

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4 10

AGRICULTURAL BIOTECHNOLOGY

1. What are enzymes ? Write characteristics of regulatory enzymes.

2. Give an account of different plant hormones and their applications in agriculture.

3. What is a gene ? Write about the fine structure and mechanism of gene regulation.

4. Justify the impact of plant genetic engineering for crop improvement.

5. What are expression vectors ? Which DNA sequences are utilized to construct expression vectors ? What are GATEWAY vectors ?

6. Give differences between the following :

(i) Type II and Type Ill restriction endonucleases (ii) RT PCR and Real Time PCR (iii) Spontaneous and induced fusion of protoplasts (iv) Northern and Western blotting

7. How and when the split genes were discovered ? Discuss the mechanism of production of a mRNA from a pllt gene.

8. What is pentose phosphate pathway ? Describe the different steps involved in it.

9. How photosynthesis can be manipulated to increase the plant yield as well as to mitigate the green-house effect ?

Describe in detail the principle of Map-based gene cloning. ✓

11. What are secondary metabolites ? Name the three important secondary metabolites being commercially produced through tissue culture. Why these secondary metabolites are not toxic to the plant cells producing them ?

12. What are biofertilizers ? Discuss about the different biofertilizers by giving suitable examples, which can be used to supplement the chemical fertilizers in agriculture.

CyTVhat are transposable elements (TEs) ? What potential role TEs have played in evolution of plants ? How TEs have helped in the functional genomics ? Explain.

14. Briefly described the salient features of BAC vector. Why it has been widely used for preparing the genomic DNA libraries ?

,z 15. A rice variety "x" has been found growing in coastal areas in Kerala. How can you identify the genes conferring salt tolerance in this variety ? g

What is allele mining ? How can it be useful in the genetic improvement of crop species ?

_17. What is MALDI-TOF ? Why is it a preferred technique for peptide sequencing ?

18. What is gene tagging and how it is different from gene targeting ? Describe how a gene of interest can be isolated by gene tagging ? _27

19. What is ribozyme technology ? Describe structural and functional differences among different types of ribozymes.

20. What is abiotic stress ? Describe the molecular basis of plant tolerance to various abiotic stresses.

21. What is molecular farming ? Describe how the plants/animals can be used as bioreactors for production of pharmaceuticals of human interest ?

briefly the contribution of the following scientists

(i) M.S. Swamhathan

(ii) Gurdev Khush

(iii) Arland Chakrabarty

List all the crops in which the genome has been completely sequenced ? Give their respective genome size. How the genome sequence can be utilized for crop improvement ? ✓

Write abrief note on new generation DNA sequencing system and the principle on which it is based.

25. Write short notes on the following :

(i) Golden rice (ii) Cry proteins (iii) Gene silencing (iv) Marker-assisted selection