ASCO Advanced Course
Ahmed Farouk Salem1,3, Niccola Funel1,2, Luca Emanuele Pollina1, Ugo Boggi2, Marco Del Chiaro2, Mosca Franco2, Generoso Bevilaqua1,
Daniela Campani1
1Division of Surgical, Molecular and Ultrastructural Pathology, 2Division of General Surgery and Transplantation. Department of Oncology, Transplants and
Advanced Technologies in Medicine, University Hospital of Pisa. Pisa, Italy.3Molecular Drug Evaluation Department, National Organization for Drug Control
and Research. Cairo, Egypt.
South & East MediterraneanCollege of Oncology
26 – 28 March 2008Cairo - Egypt
ASCO Advanced Course
20% of Pancreatic cancer patients are candidates for surgical resection.
Chemo- and radiotherapyrole remains controversialrole remains controversial.
Pancreatic cancer stillPancreatic cancer still correlates with very poor prognosis .
South & East MediterraneanCollege of Oncology
26 – 28 March 2008Cairo - Egypt
ASCO Advanced Course
South & East MediterraneanCollege of Oncology
26 – 28 March 2008Cairo - Egypt
ASCO Advanced Course
I P C-In Pancreas Cancer….
South & East MediterraneanCollege of Oncology
26 – 28 March 2008Cairo - Egypt
ASCO Advanced Course
CellCellMembrane
ds siRNA ERK1/221-23 nt
mRNAERK1/2
Electroporation Pulse
South & East MediterraneanCollege of Oncology
26 – 28 March 2008Cairo - Egypt
p
ASCO Advanced Course
20nM 80nMsiRNAsiRNA
2X
40nM
siRNAWOEP
PPCCCPP117
EP
80 M
40nMsiRNA2X
EPPP117
80nMsiRNA2X
CTRL
PPCCC: Primary Pancreatic Cancer Cell Culture EP: Electroporation CTRL: Control
siRNA ERK 1/2
South & East MediterraneanCollege of Oncology
26 – 28 March 2008Cairo - Egypt
PPCCC: Primary Pancreatic Cancer Cell Culture EP: Electroporation CTRL: Control
ASCO Advanced Course
80 nM siRNA ERK 1/2 Electroporation Only
AB against ERK1/2 AB against ATPasesiRNA ERK 1/2
with ElectroporationElectroporation Only
with Electroporation
South & East MediterraneanCollege of Oncology
26 – 28 March 2008Cairo - Egypt
ASCO Advanced Course
ERK 1/2 increases proliferation, cell survival, and resists apoptosis induced by chemotherapeutic agents.
siRNA appears to be powerful, specific inhibitor of oncogenes products.
Electroporation is cost-effective, reproducible, and rapid technique to deliver macromolecules inside cell cultures.
Examine siRNA ERK1/2 efficacy on other pancreatic cell cultures.
Determine chemo-sensitivity of pancreatic cell cultures transfected with siRNA ERK1/2siRNA ERK1/2.
Develop in-vivo electroporation technique.
South & East MediterraneanCollege of Oncology
26 – 28 March 2008Cairo - Egypt
ASCO Advanced Course
GRAZIE PER LA VOSTRAATTENZIONE
South & East MediterraneanCollege of Oncology
26 – 28 March 2008Cairo - Egypt