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Tishreen University Journal for Research and Scientific Studies - Biological Sciences Series Vol. (30) No. (2) 2008
Ascochyta fabae Speg.
��
Ascochyta fabae
Pathotypes
Ascochyta fabae
*
**
***
Ascochyta fabae Speg.
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Tishreen University Journal for Research and Scientific Studies - Biological Sciences Series Vol. (30) No. (2) 2008
A Morphological and Pathogenic Study of Ascochyta fabae Speg. Isolates collected from different Syrian
Governorates.
Ali Sbeih* Dr. Bassam bayaa **
Dr. Mathew Abang*** Dr. Mahmoud Hassan ****
Dr. Watfa Ibrahim *****
(Received 23 / 10 / 2007. Accepted 26/3/٢٠٠٨)
� ABSTRACT �
A Morphological characterization is carried out on Syrian isolates of Ascochyta fabae
collected from different Syrian governorates (Aleppo, Idleb, Hama, Lattakia, Tartous). Results reveal differences between isolates in colony color, mycelium texture, sporulation, colony diameter, and growth rate. 18% of single spore isolates show differences in replicates. It is not possible to classify isolates according to their geographical origin or the part collected from it. The patho-types of 30 isolates, taken from the same field (in Idleb), and 10 others taken from different Syrian governorates reveal the presence of 8 pathotypes, 6 of which are from one field. According to cluster analysis results, no correlation could be traced between isolates belonging to the same pathotype and their morphological properties.
Keywords: Ascochyta blight, Ascochyta fabae, Vicia faba, morphological characteristics, pathogenicity. Syria
* Post graduate student, Department of Plant Protection, Faculty , Agriculture, Tishreen University,
Lattakia, Syria **Professor, Department of Plant Protection, Faculty of Agriculture, Aleppo University, Aleppo.Syria *** Researcher, ICARDA. Aleppo.Syria ****Professor, Department of Plant Protection, Faculty of Agriculture, Tishreen University, Lattakia. Syria. *****Researcher, Agriculture scientific research center , Lattakia. Lattakia, Syria.
Tishreen University Journal. Bio. Sciences Series ٢٠٠٨) 2(العدد ) ٣٠( العلوم البیولوجیة المجلد مجلة جامعة تشرین
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Ascochyta fabae
Hanounik, 1980
Gaunt, 1983
BiotypesA. fabae
Kharbanda and Bernier, 1980; Kohpina et al., 1999
A. fabae
Kharbanda and Bernier, 1980; Ali, 1985
Bayaa and Kabbabeh, 2000
A. fabae
˚
Ascochyta fabae Speg.
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Giza 4
˚
˚
Laminar air flow
Kohpina et
al.,SarGHLat
DarTarA, B, C, D
LSP
˚
Haemocytometer Thoma
Tishreen University Journal. Bio. Sciences Series ٢٠٠٨) 2(العدد ) ٣٠( العلوم البیولوجیة المجلد مجلة جامعة تشرین
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KohpinaKohpina et al.,
ILB 1820 Giza 4 BPL 818, BPL 471
ILB 1814Hanounik and Robertson, 1989
BPL 230
ILB 463Ascot
15
Bernier et al., 1984
10.5)
3
(2-1:5
7
9
AUDPC
(Chen and Line, 1995) AUDPC= ∑[(Xi+ Xi+1)/2](Ti+1- Ti)
XiXi+1
(Ti+1- Ti)
BPL 230 ILB 142
BPL 818 ILB 549
BPL 471 ILB 287
ILB 1814
Giza 4 ILB 1820
ILB 463 NEB 463
AscotILB 1593
Ascochyta fabae Speg.
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Morphotype
A. fabae
Tishreen University Journal. Bio. Sciences Series ٢٠٠٨) 2(العدد ) ٣٠( العلوم البیولوجیة المجلد مجلة جامعة تشرین
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A. fabae
1
0.59 1
0.018 -0.002 1
0.09 0.14 0.01 1
0.03 -0.04 0.15 0.79 1
CV=235.7%
a
b
b
b
b
b
LSD
CV=243.4%
a
b
b
LSD
Ascochyta fabae Speg.
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Pathotypes
Sar 2
RS.
Pathotypes/races
Sar 2
TarGH
Sar 2-D-S5
Sar 2-D-L2.
Sar 2-C-L7GH-S
GH-L1
GH-S
Ascot
Ascot
BPL 471
Tishreen University Journal. Bio. Sciences Series ٢٠٠٨) 2(العدد ) ٣٠( العلوم البیولوجیة المجلد مجلة جامعة تشرین
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GH- L1,Tar-S1
Ascot
)Sar 2-B-P1 ،Sar 2-D-P1، Sar 2-C-L2, Sar 2-D-L3 ، Sar 2-B-P3 ،GH-S ،Dar-p1 ،Lat-P2 ،Sar 2-A-L4 ،Sar 2-B- S1 ،٢Sar 2-B-L ،Sar 1-D-L1 ،Sar 2-D-S5 ،Sar 2-D-S4 ،Sar 2-D-S1.(
BPL 471:
) Sar 2-D-S6 ، Sar 2-D-S4 ،Sar 2-D-P1 ،Sar 2-D-L1 ، Sar 2-C-L5،Sar 2-B-L2،Sar 2-B-S1، Sar 2-A-P1، Sar 2-A-L4، Sar 2-A-S1 ،Sar 2-A-L1 ،Sar 2-D-S5،Sar 2-D-S3.(
AscotSar 2-A-L3Giza 4
Sar 2-B-P3
***
BPL 230
BPL 471
ILB 1814
BPL 818
ILB 463
GIZA 4 ASCOT
Dar P4-6, Lat-P1, Sar 2-B-L1, Sar 2-C-L4, Sar 2-D-L2, Sar 2-D-L4, Sar 2-D-S5, Sar 2-D-S6,
Tar-S2
S S S S S S S 1
GH-S, Dar-P2, Lat-P2, Sar 1-B-L1, Sar 2-A-L3, Sar 2-B-P3, Sar 2-C-L1, Sar 2-C-L3, Sar 2-C-L6, Sar 2-C-L7, Sar 2-D-S2, Sar 2-D-L3, Sar 2-D-P1, Sar 2-D-S1, Sar 2-D-S3, Sar 1-D-L1
S S S S S S R 2
Sar 2-A-S1, Sar 2-S R S S S S S 3
Ascochyta fabae Speg.
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A-L2 Sar 2-A-L4, Sar 2-A-L1, Sar 2-B-S1, Sar 2-B-L2, Sar 2-B-P1, Sar 2-B-P2, Sar 2-C-L5, Sar 2-D-L1, Sar 2-D-S4
S R S S S S R 4
Sar 2-C-L2 R S S S S S R 5 Sar 2-A-P1 R R S S S S R 6
Tar-S1 S R R R S R R 7 GH-L1 R R R R R R R 8
RS
**A, B, C, D
LSP
A. fabae
%
AUDPC
AUDPC
AUDPC
AUDPC
GH-L1 15.3 0 1.2 2.1 132.2 0 4.2 28.9
GH-S 37.0 5.81 14.2 4.4 659.5 43.5 63.8 71.3
Dar-P4-6 41.2 6.19 24.3 5.3 716.6 55.8 101.2 79.9
Dar-P1 34.0 4.76 20.6 4.9 615.2 40.8 85.0 78.0
Lat-P1 47.6 6.57 31.3 5.1 836.7 61.0 144.0 82.7
Lat-P2 39.6 6.52 22.1 5.0 685.8 59.3 89.0 77.6
Sar 2-A-L1 38.9 7.42 29.2 5.3 702.0 69.0 154.5 86.9
Sar 2-A-S1 31.4 6.38 23.6 4.5 517.2 50.8 105.0 69.0
Tishreen University Journal. Bio. Sciences Series ٢٠٠٨) 2(العدد ) ٣٠( العلوم البیولوجیة المجلد مجلة جامعة تشرین
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Sar 2-A-L2 37.2 7.76 25.9 5.3 675.8 55.3 116.7 85.1
Sar 2-A-L3 37.9 7.19 28.4 5.2 641.1 65.3 125.7 81.6
Sar 2-A-L4 83.6 4.52 18.7 4.4 690.0 40.8 89.5 68.9
Sar 2-A-P1 33.1 5.14 25.2 4.1 588.0 48.6 123.7 66.9
Sar 2-B-L1 44.3 10.4 25.1 5.6 685.5 74.7 115.2 85.6
Sar 2-B-S1 30.1 5.29 19.2 4.3 520.5 45.5 77.3 65.5
Sar 2-B-L2 39.2 7.52 22.7 4.7 671.0 56.3 110.8 75.8
Sar 2-B-P1 36.4 6.95 24.3 4.7 594.9 60.3 103.2 75.8
Sar 2-B-P2 35.3 7 20.6 4.7 636.8 54.8 96.3 74.9
Sar 2-B-P3 31.4 7.38 23.2 4.9 538.9 61.8 97.5 75.8
Sar 2-C-L1 39.2 6.66 23.5 5.0 633.6 57.5 108.7 81.4
Sar 2-C-L2 31.3 5.61 22.4 4.5 527.7 51.7 101.8 70.7
Sar 2-C-L3 38.2 9.42 24.7 5.6 694.4 78.7 116.3 87.0
Sar 2-C-L4 36.4 6.14 23.1 5.0 636.1 54.2 109.2 77.6
Sar 2-C-L5 37.7 5.9 23.6 4.6 678.6 54.0 90.8 73.8
Sar 2-C-L6 40.4 7.19 23.4 5.0 732.4 60.7 107.2 84.8
Sar 2-C-L7 44.1 10 24.2 5.4 761.0 70.7 105.0 84.0
Sar 2-D-L1 24.5 0.47 4.0 3.6 251.5 2.67 14.2 44.0
Sar 2-D-S2 41.6 27.5 5.1 699.0 66.7 119.1 77.0
Sar 2D-L2 39.2 18.8 5.2 661.5 57.5 72.7 75.0
Sar 2-D-L3 42.5 32.0 5.3 770.4 92.7 156.5 87.0
Sar 2-D-L4 37.5 19.2 5.1 612.0 58.0 88.2 74.8
Sar 2-D-P1 43.9 34.7 5.4 764.2 76.5 153.8 83.9
Sar 2-D-S1 41.7 20.6 5.0 719.3 63.5 103.2 78.9
Sar 2-D-S3 34.7 2.38 15.6 4.4 503.0 14.7 67.3 64.6
Sar 2-D-S4 40.7 28.7 4.8 693.3 73.7 121.0 79.0
Sar 2-D-S5 42.4 7.90 30.6 5.0 738.7 74.8 126.3 78.1
Sar 2-D-S6 41.6 4.90 18.0 5.0 704.0 41.0 80.2 75.8
Ascochyta fabae Speg.
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Sar 1-B-L1 42.4 22.0 4.7 669.5 44.2 105.0 74.9
Sar 1-D-L1 39.2 22.5 4.9 670.2 54.0 106.2 79.7
Tar-S1 16.5 0 0.0 2.3 106.8 0 0 28.9
Tar-S2 46.4 40.9 5.7 868.1 102.2 192.2 93.2
AUDPC
AUDPC
AUDPC
AUDPC
AUDPC
%
*
1
AUDPC
0.935
1
AUDPC
0.913
0.948 1
AUDPC
0.81 0.907 0.84 1
AUDPC
0.812
0.895 0.886 0.84 1
AUDPC
0.846
0.92 0.792 0.89 0.816 1
0.801
0.843 0.837 0.756 0.952 0.755 1
%
0.761
0.55 0.666 0.44 0.46 0.438 0.449 1
Tishreen University Journal. Bio. Sciences Series ٢٠٠٨) 2(العدد ) ٣٠( العلوم البیولوجیة المجلد مجلة جامعة تشرین
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0.814
0.91 0.843 0.98 0.855 0.897 0.774 0.443 1
0.85 0.91 0.79 0.87 0.83 0.97 0.78 0.44 0.87 1
*(Disease index)
Ri
Ascochyta fabae Speg.
١٧٦
Ascochyta fabaeKohpina
et al.,1999
Tivoli and Maurin,1992
Kohpina et al.,1999
Tivoli and Maurin,1992
Kharbanda and Bernier
Kohpina et
al.,1999
Ali, 1985
Rashid et
al., 1991Kohpina et al., 1999Hanounik and Robertson, 1989
A. fabaeKharrat et al.,
2000
Yakop et al., 1997
Rashid et al., 1991Kharrat et al., 2000
Tishreen University Journal. Bio. Sciences Series ٢٠٠٨) 2(العدد ) ٣٠( العلوم البیولوجیة المجلد مجلة جامعة تشرین
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A.
fabae
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Ascochyta fabae Speg.
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