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Attack of the Superbugs Lab Introduction

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Attack of the Superbugs Lab Introduction. Mrs. Stewart Medical Interventions. Superbugs. What is a “superbug”? Bacteria that have become stronger and less responsive to antibiotic treatment. How can bacteria share resistance?. This lab uses 2 types of Bacteria:. E. coli I - PowerPoint PPT Presentation
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Attack of the Superbugs Lab Introduction Mrs. Stewart Medical Interventions
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Page 1: Attack of the Superbugs  Lab Introduction

Attack of the Superbugs Lab Introduction

Mrs. StewartMedical Interventions

Page 2: Attack of the Superbugs  Lab Introduction

Superbugs• What is a

“superbug”?– Bacteria that

have become stronger and less responsive to antibiotic treatment

Page 3: Attack of the Superbugs  Lab Introduction

How can bacteria share resistance?Genetic Transfer

MethodDescription

Conjugation The one-way transfer of DNA (plasmid) between bacteria in close

cellular contact

Transformation The genetic modification of a bacterium by incorporation of free

DNA from the surrounding environment (usually caused by another ruptured bacterial cell)

Transduction The transfer of genetic material from one bacterium to another by a genetic

vector (bacteriophage virus)

Page 4: Attack of the Superbugs  Lab Introduction

This lab uses 2 types of Bacteria:

• E. coli I – contains a gene found on the chromosomal DNA

coding for streptomycin resistance• E. coli II

– contains a gene found on the plasmid DNA coding for ampicillin resistance.

Page 5: Attack of the Superbugs  Lab Introduction

Predict

• What will happen when the E. coli I strain is mixed with the E. coli II strain?

Page 6: Attack of the Superbugs  Lab Introduction

Lab Materials: 4 growth plates

LB Agar

LB + Amp

LB + Str

LB /Str /Amp

*LB Agar = growth medium for bacterial cells

Page 7: Attack of the Superbugs  Lab Introduction

Lab Day 1:

• Obtain 2 of each type of growth plate• Streak E. coli I on 4 plates• Streak E. coli II on 4 plates• Incubate overnight

*These will be the confirmation plates

Page 8: Attack of the Superbugs  Lab Introduction

Evaluate

• What are we confirming?

Page 9: Attack of the Superbugs  Lab Introduction

Lab Day 2:

• Observe confirmation plate results• Prepare “mix” plate

– You will literally be mixing the two types of bacteria together onto one plate

• Incubate overnight

Page 10: Attack of the Superbugs  Lab Introduction

Evaluate

• Why are we mixing them together?

Page 11: Attack of the Superbugs  Lab Introduction

Lab Day 3:

• Use new “mixed” bacterial cultures and spread onto the 3 types of antibiotic plates

Page 12: Attack of the Superbugs  Lab Introduction

Predict• What would you expect to see on

these growth plates?• Why?

Page 13: Attack of the Superbugs  Lab Introduction

FRIDAY – Lab Day 4

• You must come in at some point during the day Friday to observe and record your results

• Remember: Pictures of lab results will be required in your lab reports

*Growth plates will be discarded at 3:10 pm on Friday. Any results not recorded will be lost.

Page 14: Attack of the Superbugs  Lab Introduction

Student Lab ScheduleDay: Time Needed: Activity: How to Store: Additional Info:

Lab Day 1 ~ 30 minutes Students prepare confirmation plates.

Invert the plates, label, and incubate at 37˚C for 24 hours.

(1) Students complete steps 1 – 33 on P.1.2.2.(2) Students work through the mechanisms of antibiotic resistance animations.

Lab Day 2 ~ 30 minutes Students observe confirmation plates and prepare “Mix Plate.”

Invert the plates, label, and incubate at 37˚C for 24 hours.

(1) Students complete steps 34 – 47 on P.1.2.2. (2) Students begin construction of 3-D model.

Lab Day 3 ~ 30 minutes Students streak cultures from “Mix Plate” onto antibiotic plates.

Invert the plates, label, and incubate at 37˚C for 24 hours.

(1) Students complete steps 48 – 60 on P.1.2.2.(2) Students continue construction of 3-D model.

Lab Day 4 ~ 10 minutes Students make observations of plates to test for antibiotic resistance.

Clean-up all materials.

(1) Students complete steps 61 – 65 on P.1.2.2.(2) Students complete work on 3-D models and present models to class.(3) Students answer all remaining Conclusion questions.


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