Date post: | 07-May-2015 |
Category: |
Technology |
Upload: | abiotech |
View: | 1,589 times |
Download: | 6 times |
THE FOLLOWING
QUESTIONS!
a s k
your-
s e l f
1
HAVE ENZYMES EVER
BEEN A LIMITING FACTOR IN
YOUR PROCESSES?
HAS INTRACELLULAR
EXPRESSION OF ENZYMES
BEEN A BOTTLENECK?
2
HAVE YOU EVER
WISHED, TO BE ABLE TO
RECOVER ENZYMES FROM
YOUR PROCESSES?
3
WAS IMMOBILISATION OF
ENZYMES DISCARDED BECAUSE
OF HIGH COSTS?
4
ONETHERE IS
OPTION
COMBINING THE POSITIVE
CHARACTERISTICS OF
free
enzymes
whole cell
biocatalysts
immo-
bilisation
WHILE RETAINING
MAXIMUM FLEXIBILITY
bacterial
surface
display
NATURAL IMMOBILISATION OF
ENZYMES THROUGH…
SP PASSENGER LINKER β-BARREL
IM
OM
PP
CP
OUR TECHNOLOGY…
IM
OM
PP
CP
SP PASSENGER LINKER β-BARREL
… IS A SELF-SUFFICIENT SYSTEM
WHAT ARE THE BENEFITS
simply
centrifuge or
filter
FOR A COST-EFFICIENT
PURIFICATION OF ENZYMES…
1
FOR THE RECOVERY OF ENZYMES
FROM A CONVERSION PROCESS…
2
simply
centrifuge or
filter
100%
#5#1
CYCLES
@24h each#2 #3 #4
CONVERSION
NITRILASE EXPERIMENTS PROVE:
RECOVERY IS SUCCESSFUL
SCHEMATIC DRAWING
70%
50%
30%
10%
UNLOCK ENHANCED ENZYME
STABILITY THROUGH…
3natural
immobilisation
smart
choice of host
organisms
ENABLE METABOLIZATION OF
ENZYME INHIBITORS THROUGH…
4
integrated
cleavage sites
(& HIS-tag)
RELEASE ENZYMES EXPRESSED
WITH AUTODISPLAY THROUGH…
5
resuspendent
cells
SUPERNATANT
HIGH CELL DENSITY
FERMENTATION
CONCENTRATED
SUPERNATANT
CONCENTRATION OF RELEASED
ENZYMES IS SIMPLE
FILTRATE1 2 N
HOW MANY
MOLECULES ARE LOCATED
ON THE SURFACE?
you
may
a sk
MOLECULES/CELL
100,000 200,000–
WE CAN HELP, WHEN
OTHER SYSTEMS FAIL
ESPECIALLY IN THE CASE OF
CHALLENGING ENZYMES
WHAT ARE
CHALLENGING
ENZYMES?
you
may
a sk
inclusion
bodies
multimeri-
sation
incorrect
folding
co-factors
lack of
stability
ALL ENZYMES WHICH ARE
DIFFICULT TO HANDLE, DUE TO…
+
ENZYME PROBLEM NAME THE ENZYME GENE SEQUENCE
GACTTCGAAGTC
TTTAAGAATGACT
TCGAAGTCTTTAT
PLASMIDHOST
ORGANISM
gram –
WHOLE CELL
BIOCATALYST
1 2
3
4
WE ARE DEDICATED TO SOLVE
BIOCATALYTIC BOTTLECKS
enzyme
recovery/
re-use
enzyme
stability
enzyme
purification
enzyme
portfolio
enzyme
costs
sales and
profitability
RECAP: VALUE PROPOSITION
TALK TO US, IF YOU
WANT TO KNOW MORE