TB‐dx Final Design
J. Bentley, K. Cooley, D. Crowe, M. Evans, L. Sehgal
University of Rochester
Department of Biomedical Engineering
05/06/2009
Compiled for Benjamin Castaneda and Fanny Casado by Automated
Diagnostics under the supervision of Edward Brown III, Ph.D.
Outline Problem Statement
Customer Scenario
Standard of Care in Peru
Overall System Design
Division of Tasks
Design Process
Final Design
Future Prospects
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The Issue
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Medicine World
Customer Scenario Hospital Nacional Dos de Mayo
in Lima, Peru
Current Situation in Peru
TB is a serious public health threat in Peru
44% of the population is below the poverty line
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Standard of Health Care in Peru Tuberculosis Diagnostic Procedure
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Image source: PUCPDigital image of Bacilloscopy
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Overall System Design
TB-dx Setup NEEDS NEW PIC!!!
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User Interface Quick Summary of user interface…
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Pontificia Universidad Catolica del Peru Image processing and
detection of mycobacteria
Count bacilli
Future: Auto Focusing
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Image Processing Team from PUCP
University of Rochester
Receive output # of bacilli from PUCP
Automate stage movement for 1 slide
Quantify/qualify focus problems
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Automated Diagnostics
Experimental Results Conducted experiments to determine the sensitivity of
focus across a slide with 100X oil lens
Used parallel slices of rat femur and uneven human TB-free sputum samples to quantify
Attempted to move as many millimeters as possible until the sample was out of focus
On average, human samples had to be re-focused every millimeter
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Prototype Iteration #1
Flat slide bed Rubber band belts
Plastic motor housing
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Prototype Iteration #2
Multi-layered slide bed
Rubber band belts
Machined aluminum motor housing
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Final Design: TB-dx
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Final Design: TB-dx
Existing slide holder on Olympus CX31 microscope stage
Flat NBR‐Coated Nylon Fabric Belts
Machined aluminum motor housing
NEED PICTURE WITH NEW BELTS HERE
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Olympus CX31 Manual
Future Prospects Multiple Slides
Automated Focus
Compatibility
Zeiss
Computer Free Product
Entire System Testing
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Carl Zeiss’ Primo Star iLED fluorescence microscope
Thank You
• Ben Castaneda
• Fanny Casado
• Professor Ed Brown
•
• Art Salo
• Paul Osborne
• Professor Scott Seidman
• Professor Amy Lerner
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