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Automated Mini-StoreSystem forLow-Temperature Samples Storage
Lab Automation solutions
by PAA/Biosero
Focused offerings for high-throughput screening applications
Automated Samples Storage Systems
• Range of capacities and functionalities
• Ultra-low temperature robotic sample storage
• Automation integration – Front/back-end
Controlled-Environment Chambers
Targeted for manual and automated work-processes
Dry, inert, oxygen-free, ionized, temperature-controlled atmospheres possible
Dry, nitrogen atmosphere for automated liquid-handling
workcell
Dry, nitrogen atmosphere for samples weighing
Automated Mini-Store (AMS)
For small-medium libraries
• Standalone, self-contained, mobile• Configurable for range of tubes and vials• Cherry-picking capability• Medium-capacity; expandable• Modular design
General features
AMS Features and Specifications 1/2
• System Size: 8’ wide, 5’ deep, 6.5’ tall• Capacity: 300,000 micro-tubes (0.7ml)• Throughput: 4000 picks/day; higher possible• Cherry-picking: Yes, for 96- and 384-well• Operating environment:
o +4ºC to -20 º C (settable)o -80 º C option available
• Environment Backup: Liquid Nitrogen
AMS Features and Specifications 2/2
• Input/Output Station: Positions for 6 racks• Output Stacker: 24 racks capacity• Compression/Consolidation:
o On-demand or auto• Database: Any SQL database• Samples ID: 2D matrix and 1D barcode
Container Types and Capacity
96-well Tubes, Half-height (0.7 ml)
175,000 tubes (max 190,000)
96-well Tubes, Standard-height (1.4 ml)
275,000 tubes (max 300,000)
Picoplates (or Microtiter plates)
1.9 Million picotubes2.6 Million in stacked configuration
Other Container Types
Vials, custom racks and tubes are possible
Multi-Container Types in Single AMS
AMS can easily be configured to simultaneously handle multiple container types in a single system.
This is achieved by using gripper-changers at the Picking Robot for handling each container-type (similar to our larger ASRS)
Note, however, that this affects the overall throughput of the system (and some loss of flexibility)
Modules View – Main Discrete Modules
Storage Module #1
Storage Module #2
Picking Module
Input/Output Module
Modules View – Storage Stack
Storage Stack
Single Pan
Modules View – Picking Module
Extractor
Extractor transfers product from storage module to picking module
Picker
Picker (robot) cherry-picks samples from source-racks to destination racks
Modules View – Pan in Extractor
Picking Module – Picking Station
I/O Buffer
I/O Buffer can hold 6 racks
Cherry-Picker
Can pick tubes and vials of various sizes
Pan Extracted for Picking
Extractor
Picker
Source Rack
I/O Module
Modules View – I/O Module
System display
I/O Stacker
Rack Scanner
Dry Inert enclosure for plate thawing
• Inert / dry plate exit hotel• Dry Nitrogen atmosphere• Room Temperature
thawing• Recirculation with inline
warming & desiccating module.
• Intelligent control with O2 and humidity sensors to monitor environment
• Minimal N2 consumption
Sample Retrieval from the Inert hotel
The available capacity of the Hotel allows for batch loading of tube racks into the AMS.
Tube racks can be efficiently handled in batches using ‘Cassettes’, with a capacity of 10 tube racks each.
AMS
InertPlateHotel
Automated transfer oftuberacks
Manual transfer
of tuberacks inCassettes
(Option) Tuberacks singly loaded/unloaded
into AMS manually
Tuberacks (in Cassettes) loaded/unloaded into AMS manually
Airlock
The antechamber allows product transaction without compromising the internal atmosphere
Cycle Times
AMS Cycle Time as a Function of Pan Extractions
2.0
3.0
4.0
5.0
6.0
0.0 24.0 48.0 72.0 96.0
# of tuberack picks/pan
Cyc
le t
ime
(tu
ber
acks
/hr)
The adjacent graph shows the dependency of cycle-time to picking efficiency. If the inventory is fragmented, or if pick request is characterized by dispersed samples, the throughput will be lower.
For pan extractions in the range of 1 to 20, the steep slope of the graph underlines the advantages of condensed/optimized storage.
Important note: these numbers are for serial service of tube racks (not picked in batches, which AMS can do, up to 6 tube racks at once). With batch picking, throughput will be higher.
AMS System Features
Feature RANDEX AMS
Throughput Cherry-pick of 4 96-well tube racks per hour, on average (see conditions)
Temperature Yes, settable between +4C and –20C
Redundancy/backup In case of power failure or refrigeration system failure, liquid nitrogen backup (option)
Humidity Control Built-in dehumidification control
Container Types, Capacities
300,000 0.65ml Micronic micro-tubes in their racks: (height: 32 mm); 267,264 0.75ml Matrix micro-tubes in their racks: (height: 40 mm)
Cherry-Picking Micro-tubes are cherry-pickable. Cherry-picking is done inside the controlled environment
System Size and weight AMS Standard Size: 8’ wide, 5’ deep, and 6.5’ tall. Weight: 2,500 lbsAMS with Integrated Hotel:
Barcode scanning 1D barcode scanning of racks. 2D cluster scanner for identification of microtubes
Input/Output AMS Standard via front stacker (24 tube racks), or serially.AMS with Integrated Hotel: automatic transfer of tube racks to and from Liconic LPX-200 Hotel. Hotel capacity is 100 tube racks
Compression/ Consolidation
Micro-tube compression. Can also compress by custom-defined field (project/department)
Inventory Data Will reside in your enterprise database or in our MSDE (SQL) database
Service Support 24 hour turnaround in support and service
Process – Storing Plates/Racks
Store Request (via user or software agent)
Move Rack(i) from stacker to ID areaRead 2D datamatrix
Transfer Rack(i) from ID area to I/O Shuttle
Picker moves Rack(i) from shuttle to I/O_Nest (i)
repeat until all Racks doneOR I/O_Nest is full
Extractor retrieves Pan(i) to I/O Station
Picker transfers Rack(i) to Pan(i)
repeat until all Racks done
Repeat until done
Process for Storing Racks with 2D ID underside
Process – Retrieving Samples
Retrieve Request (via user or software agent)
Check Destination Rack (DR) existsCheck requested samples are in inventory
Check parameters within physical limits
Extractor moves Pan(i) to I/O Station
Picker picks Tube(i) from Source Rack (SR) to DR
Picker transfers Rack(i) to Pan(i)
repeat until all req. tubesare picked from Pan(i)
Extractor returns Pan(i) to Store
repeatuntildone
Picker moves DR(i) to ID Station2D Datamatrix is verifiedDR(i) is moved to Stacker
Software/Data Integration to LIMS
Enterprise database (RDBMS)
User (and Security) ManagerProduct Data ManagerTransactions Manager
Product Input-Output ManagerMachine Control Positions Data Manager
Machine Control CommunicationsManager
ReportingInterface
UI for datafile upload
Business objects
Samples Login System(SLS)
Supervisory Control System(SCS)
Hosted web-serviceOR
Active or Passive Ordering System
(client-specific)
Global Samples Ordering System(GSOS)
We can integrate our systems seamlessly into your LIMS
Data Model
Inert Sample ProcessingHuman and Robotic Solutions
A Precipitous Problem
DMSO Weight Gain in Laboratory
100
105
110
115
120
125
0:00 12:00 0:00 12:00 0:00
Perc
en
t
WebData from Labcyte.comwater absorption vs. time (hrs)
WebData from Tekcel.comwater absorption vs. time (hrs)
• Samples absorb 10% water in 4 hours under ambient conditions• This will prevent freezing @ 20 C and compound precipitation• Sample precipitation occurs under such sub-optimal conditions• HTS and hit-to-lead follow-up are compromised• Consensus is that dry sample processing is a component of valid compound management for HTS
WebData from Waybright et al., NCIsample precipitation
Precipitation = More False Negatives
How Much Does a False Negative Cost?
Opportunity and Operations Loss
Solutions to a Common Problem Cost:Benefit
• Good: Dry boxes for thawing plates• Better: Semi-automated processing in inert
enclosure• State of the Art: ASRS and Integrated Workcell
Summary: Dry Box Solution
Summary: Dry Box Solution
• Obligatory for thawing/conditioning plates• Rapid dry equilibration of chamber• Gentle, ‘Power Thaw’ option• <1% Relative Humidity achievable• Requires only power outlet• Movable, space efficient and affordable• Vastly superior to passive desiccant • Custom configurations and options available
1/10 cost of alternative solutions by redeploying existing equipment
InputAirlock
Mobile(casters)
Heightadjustable
Pipettor Sealer
Outputairlock
Stackers
Glove ports
Inert/dryatmosphere
controls
Semi-Automated (‘Hubotic’) Solutions
Summary:‘Hubotic’ Solution
• Powerful workflow model. Simple and very cost-effective• Pressurized with dry argon gas, data monitored and logged• Inert processing from solubilization to seal• Movable, ergonomically adjustable• Continuous Batch Processing without breaking the conditioned environment
Turnkey System to your specifications Compact, movable, cost-effective From an established leader in Materials Management
Fully Integrated Robotic Solution
Automated Mini-Store
(AMS)Plates Processing Workstation
(PPW)
Buffer Stack for Random Access (of 384-well Plates)
Transfer Robot
Buffer Stack for Empty Plates
Buffer Stack for Thawing Racks
Shuttle between AMS and PPW
Liquid Handling SystemAir-Lock
Operational Schematic
PlateLoc Plate Sealer
Compound Management Solutions
• Samples must be processed in dry environmento Simple solution is the DB500 for manual processingo prevents water absorption during plate thawing
• Continuous Batch Processing with Enclosureo emulates robotic solution (‘Hubotic’) at low cost
• Fully Integrated AMS: Best of both worldso Turnkey ASRS integrated turnkey sample processingo Attractively priced, compact footprint
• Service and support• Tried and tested technology
Appendix - (384-well) Picoplate
• Field-proven, patented design• Source plate is single molded
part – Low cost• Cherry pick capability – no
freeze-thaw cycle
Source Plate
Bite and Place
Destination plate