1 Avtar Krishan Handa Center for Plant Environmental Stress Physiology Department of Horticulture and Landscape Architecture Purdue University 625 Agriculture Mall Drive, West Lafayette, IN 47907-2010 Phone: (765) 494-1339 Fax: (765) 494-0391 E-mail: ahanda@ purdue.edu Education Dates Degree Institution Subject 1977 Ph.D. Tata Institute of Fundamental Research, Mumbai Molecular Biology 1970 M.Sc. Aligarh University, Aligarh Organic Chemistry 1968 B.Sc Punjab University, Chandigarh Chemistry, Physics, Mathematics Positions Dates Title Organization 1991-present Professor Purdue University, Dept. Horticulture, West Lafayette, IN 1985-1991 Associate Professor Purdue University, Dept. Horticulture, West Lafayette, IN 1981-1985 Assistant Professor Purdue University, Dept. Horticulture, West Lafayette, IN 1979-1981 Research Associate Purdue University, Dept. Horticulture, West Lafayette, IN 1978-1981 Research Associate Purdue University, Dept. Botany and Pathology West Lafayette, IN 1977-1987 Research Associate Michigan State University, DOE laboratory, E. Lansing, MI 1970-1977 Research Scholar Tata Institute of Fundamental Research, Mumbai, India Honors andAwards 2004 Elected Fellow of the World Innovation Foundation 2003 ISI Highly Cited Author: Recognized by Institute of Scientific Information [ISI] as one of the highly cited authors in the field of Animal and Plant Biology. 2003 Nominated for the 2003 Biotechnology award by World Technology Network in association with NASDAQ, TIME magazine, AAAS, Science magazine, and Technology Review magazine 2003 Development of high lycopene tomato recognized as best in biotechnology research in the USA by Roper survey 1970 Gold Medal First Rank in University-MS Chemistry Memberships Dates Organization Position Since 1977 American Society of Plant Biology Life member Gamma Sigma Delta Member Professional Service Member NSF panel, the U.S. Centers for Science and Technology. Co-Chairman BARD 1989-1990 panel for Postharvest and Food Science.
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Avtar Krishan Handa Center for Plant Environmental Stress Physiology
Department of Horticulture and Landscape Architecture Purdue University
625 Agriculture Mall Drive, West Lafayette, IN 47907-2010 Phone: (765) 494-1339 Fax: (765) 494-0391
E-mail: ahanda@ purdue.edu Education Dates Degree Institution Subject 1977 Ph.D. Tata Institute of Fundamental Research, Mumbai Molecular Biology 1970 M.Sc. Aligarh University, Aligarh Organic Chemistry 1968 B.Sc Punjab University, Chandigarh Chemistry, Physics, Mathematics Positions Dates Title Organization 1991-present Professor Purdue University, Dept. Horticulture, West Lafayette, IN 1985-1991 Associate Professor Purdue University, Dept. Horticulture, West Lafayette, IN 1981-1985 Assistant Professor Purdue University, Dept. Horticulture, West Lafayette, IN 1979-1981 Research Associate Purdue University, Dept. Horticulture, West Lafayette, IN 1978-1981 Research Associate Purdue University, Dept. Botany and Pathology West Lafayette, IN 1977-1987 Research Associate Michigan State University, DOE laboratory, E. Lansing, MI 1970-1977 Research Scholar Tata Institute of Fundamental Research, Mumbai, India Honors andAwards 2004 Elected Fellow of the World Innovation Foundation 2003 ISI Highly Cited Author: Recognized by Institute of Scientific Information [ISI] as one
of the highly cited authors in the field of Animal and Plant Biology. 2003 Nominated for the 2003 Biotechnology award by World Technology Network in
association with NASDAQ, TIME magazine, AAAS, Science magazine, and Technology Review magazine
2003 Development of high lycopene tomato recognized as best in biotechnology research in the USA by Roper survey
1970 Gold Medal First Rank in University-MS Chemistry Memberships Dates Organization Position Since 1977 American Society of Plant Biology Life member Gamma Sigma Delta Member Professional Service Member NSF panel, the U.S. Centers for Science and Technology. Co-Chairman BARD 1989-1990 panel for Postharvest and Food Science.
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Co-Chairman BARD 1990-1991 Panel for Postharvest and Food Science. Co-Chairman BARD 1998-1999 Panel for Field and Garden Crops. Chairman BARD 1999-2001 Panel for Field and Garden Crops. USDA/NRI 2000 Grant review panel for Food Characterization. Chairman (2003-2004) Search committee for Plant Biochemical and Molecular Genetics Position USAID (2003-2006): Member the MUCIA Biotechnology team to evaluate the biotechnology program
in Egypt USAID: 2004-2005 Panel for the Midwest Universities Consortium for International Activities, Inc.
(MUCIA) Biotechnology Proposal Evaluation Facilitator- USAID Synthesis workshop on Global Horticulture Challenges October 18 & 19,
2004. Davis California USAID 2005 Panel for the Impact Grant Livestock proposal evaluation Egypt, MUCIA, AERI
Institutional Linkage Component Member Organizing committee (2005) International Conference on Biotechnology Approaches
to Alleviating Malnutrition and Human Health..University of Agricultural sciences, Bangalore , India, January 9-11, 2006.
Team Member (2006) to develop a Guidelines and Considerations for Unintended Release of Transgenic plants. Donald Danforth Plant Science Center, St. Louis.September 8, 2006
Member Organizing committee (2007). International conference on21 ST Century Challenges of Sustainable Agricultural Systems – Biotechnology, Environment, Nutrition, Trade and Policy 15th-17th March 2007
Editorial: Reviewer for USDA, NSF, DOE, BARD, NIH and NRC Canada proposals;
Reviewer for Plant Cell, Proceeding of National Academy of Sciences, USA, Plant Physiology, Can. J. Bot, Horticulture Reviews, Plant Breeding Reviews., Physiologia Plantarum, J. Food Sciences, HortScience, J. Amer. Soc. Hort. Sci.
Research Interests Field of expertise & Molecular regulation of plant growth and development Research Interests Postharvest biology and fruit ripening Molecular engineering of fruits to enhance quality attributes Modulating heat stress to enhance crop productivity Production of Vaccines in Plants Brief description of research program
The emphasis of my research is the mechanisms regulating gene expression during fruit ripening and development. Additionally I am interested in discovery of genes that control desirable attributes of fruits. The projects being perused in my laboratory include identifying genes that regulate fruit shelf-life, texture, ripening and processing quality. Other important focus of his lab is to identify mechanisms that regulate levels of antioxidants, minerals and vitamins in ripening fruits. We are also perusing role of molecular modification in enhancing antigenicity of vaccine molecules. Some of recent accomplishments include: 1) Engineering over 3-fold increase in the levels of lycopene in tomato fruits. Lycopene has been the focus of much attention since 1995, as it has been shown to reduce risk of developing prostate cancer by 45 % and several other cancers by about 20 %. 2) Modifying pectin chemistry by down-regulating levels of pectin methylesterase and polygalacturonase in tomato to enhance juice viscosity and
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net processing yield. 3) Characterization of heat stress response in order to improve heat tolerance and pollination in tomato. We have identified and characterized effects of a novel small heat shock protein gene, designated as vis1 (viscosity 1), that regulated tomato juice viscosity. 4) Mechanisms regulating homeostasis in fruit to maintain fruit texture. Our results show that down regulation of a fruit ripening associated lipoxygenase reduces ion leakage and enhances fruit firmness (US patent 6355862). 5) Expressed avian flu vaccine gene in both tomato and tobacco plants and cell cultures. We have developed a tomato fruit macroarrays to characterize global changes in fruit gene expression under various growth conditions, including stress. We are using this array to characterize gene whose expression is modified in ‘yellow shoulder disorder in tomato. Other interests include developing and characterizing a transposon insertion mutant population of Microtom, a dwarf tomato variety. This project would likely identify many genes of potential interest to improve production and overall quality, including levels of vitamins and mineral, in fruit and vegetable crops. Teaching Courses taught:
HORT 335, Handling, Processing and Distribution of Horticultural Crops HORT 541. Postharvest Technology of Fruits and Vegetables HORT 590A/FS591A. Postharvest Technology of Fruits and Vegetables: HORT 590M Research Methods HORT 602. Experimental approaches to Plant Science Research. BIOL/BOT/HORT 650B Postharvest Physiology HORT 652G Postharvest Physiology and Senescence HORT 690S Transgenic Plants
Guest lecture in HORT 553. "Plant Growth and Development". Lecture Title: "Dissection of Plant Growth and Development Using Antisense RNA".
Guest lecture in HORT 690A. "Plant Stress Physiology". Lecture Title: "High temperature effects on growth and physiology of plant tissues".
HORT 590G: Plant Growth and Developmnt. Two guest lectures entitled: 1) Fruit structure and ripening and 2) Biochemical changes and molecular biology of fruit ripening.
HORT 491: Horticulture Production Technology. Two guest lectures entitled: 1) Introduction to postharvest biology and 2) Postharvest technology
Refereed Publications: Handa, A.K. and M.M. Johri (1976) Cell differentiation by 3', 5' - cyclic AMP in a lower plant.
Nature 259:480-482. Handa, A.K. and M.M. Johri (1977) Cyclic 3',5'-adenosine monophosphate in the moss
protonema. A comparison of its levels by protein kinase and Gilman assays. Plant Physiol. 59:490-496.
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Handa, A.K. and M.M. Johri (1979) Involvement of cyclic adenosine 3', 5'-monophosphate in chloronema differentiation in protonema cultures of Funaria hygrometrica. Planta 144:317-324.
Hood, E.E., S. Armour, J.D. Ownby, R.A. Bressan and A.K. Handa (1979) Adenonine 3',5'-cyclic monophosphate in Anabaena variablis: Effect of nitrogen starvation. Biophys. Bochem. Acta. 588:193-200.
Larkins, B. A., K. Pedersen, A. K. Handa, W. J. Hurkman and L. D. Smith (1979) Synthesis and processing of maize proteins in Xenopus laevis oocytes. Proc. Natl. Acad. Sci., USA 76:6448-6452.
Handa, A.K. and R.A. Bressan (1980) Assay of adenosine 3', 5'- cyclic monophosphate by stimulation of protein kinase: A method not involving radioactivity. Analytical Biochem. 102:332-339.
Bressan, R.A., A.K. Handa, J. Cherniack and P. Filner (1980) Synthesis and release of (32p) - adenonine 3': 5'-cyclic monophosphate by Chlamydomonas reinhardtii. Phytochemistry 19:2089-2093.
Bressan, R.A., A.K. Handa, M. Quader, and P. Filner (1980) Synthesis and release of 3': 5' cyclic monophosphate by Ochromonas malhamensis. Plant Physiol. 65:165-170.
Hasegawa, P.M., R.A. Bressan and A.K. Handa (1980) Growth characteristics of NaCl selected and nonselected cells of Nicotiana tabacum L. Plant and Cell Physiol. 21:1347-1355.
Bressan, R.A., P.M. Hasegawa and A.K. Handa (1981) Resistance of cultured higher plant cells to polyethylene glycol induced water stress. Plant. Science Letters 21:23-30.
Handa, A.K., R.A. Bressan, M. Quader and P. Filner (1981) Association of formation and release of cyclic AMP with glucose starvation and subsequent chlorophyll synthesis in Ochromonas malhamensis. Plant Physiol. 68:460-463.
Handa, A.K., R.A. Bressan, M.L. Park and P. M. Hasegawa (1982) Use of plant cell cultures to study production and phytotoxicity of Alternaria solani toxin(s). Physiological Plant Pathology 21:295-309.
Handa, A.K., R.A. Bressan, S. Handa, and P.M. Hasegawa (1982) Characteristics of cultured tomato cells after prolonged exposure to medium containing polyethylene glycol. Plant Physiol. 69:514-521.
Bressan, R.A., A.K. Handa, S. Handa and P.M. Hasegawa (1982) Growth and water relations of cultured tomato cells after adjustment to low external water potentials. Plant Physiol. 70:1303-1309.
Jayaswal, R.K., R.A. Bressan, P.M. Hasegawa and A.K. Handa (1983) Occurrence of cyclic AMP in the phytopathogenic fungi Alternaria solani and Phymatotrichum omnivorum. Archives of Microbiology. 135:125-129.
Handa, A.K., R.A. Bressan, S. Handa and P.M. Hasegawa (1983) Clonal variation for tolerance to PEG-induced water stress in cultured tomato cells. Plant Physiol. 72:645-653.
Handa, S., R.A. Bressan, A.K. Handa, N.C. Carpita and P.M. Hasegawa (1983) Solutes contributing to osmotic adjustment in cultured plant cells adapted to water stress. Plant Physiol. 73:834-843.
Hasegawa, P.M., R.A. Bressan, S. Handa and A.K. Handa (1984) Cellular mechanisms of tolerance to water stress. HortSci. 19:371-377.
Jayaswal, R.K., R.A. Bressan and A.K. Handa (1984) Mutagenesis of Erwinia carotovora subsp. carotovora with bacteriophage Mu d1 (Apr lac cts62): Construction of his::lac gene fusion. J. Bacteriol. 158:764-766.
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Jarret, R.L., E.C. Tigchelaar and A.K. Handa (1984) Ripening behavior of the greenripe tomato mutant. J. Amer. Soc. Hort. Sci. 109:712-717.
Handa, A.K., N.K. Singh and M.S. Biggs (1985) Effect of tunicamycin on in vitro ripening of tomato pericarp tissue. Physiol. Plant. 63:417-424.
LaRosa, P.C., A.K. Handa, P.M. Hasegawa and R.A. Bressan (1985) Abscisic acid accelerates adaptation of cultured tobacco cells to NaCl. Plant Physiol. 79:138-142.
Binzel, M.L., P.M. Hasegawa, A.K. Handa and R.A. Bressan (1985) Adaptation of tobacco cells to NaCl. Plant Physiol. 79:118-125.
Singh, N.K., A.K. Handa, P.M. Hasegawa and R.A. Bressan (1985) Expression of proteins associated with adaptation of cultured tobacco cells to NaCl. Plant Physiol. 79:126-137.
Jayaswal, R.K., R.A. Bressan and A.K. Handa (1985) Adenylate cyclase from phytopathogenic fungus Alternaria solani. FEMS Microbiol. Letters. 27:313-318.
Jayaswal, R.K., R.A. Bressan and A.K. Handa (1985) Behavior of bacteriophage P1 in Erwinia carotovora. Current Microbiol. 12:73-78.
Jayaswal, R.K., R.A. Bressan and A.K. Handa (1985) Effects of a mutation that eliminates UDPG-pyrophosphorylase on the pathogenicity of Erwinia carotovora subsp. carotovora. J. Bacteriol. 164:473-476.
Jayaswal, R.K., R.A. Bressan, D.J. Charles and A.K. Handa (1986) Studies on Inc-P plasmids in Erwinia carotovora subsp. carotovora. FEMS Microbiol. Letters. 35:307-312.
Handa, S., A.K. Handa, P.M. Hasegawa and R.A. Bressan (1986) Proline accumulation and the adaptation of cultured plant cells to water stress. Plant Physiol.. 80:938-945.
Biggs, M.S., R.W. Harriman and A.K. Handa (1986) Changes in gene expression during tomato fruit ripening. Plant Physiol. 81:395-403.
Hasegawa, P.M., R.A. Bressan and A.K. Handa (1986) Cellular mechanisms of salinity tolerance. HortSci. 21:1217-1324.
Binzel, M.L., P.M. Hasegawa, D. Rhodes, S. Handa, A.K. Handa and R.A. Bressan (1987) Solute accumulation in tobacco cells adapted to NaCl. Plant Physiol. 84:1408-1415.
Singh, N.K., C.A. Bracker, P.M. Hasegawa, A.K. Handa, S. Buckel, M.A. Hermodson, E. Pfankoch, F.E. Regnier and R.A. Bressan (1987) Characterization of Osmotin: (A Thaumatin-like protein associated with osmotic adaptation in plant cells. Plant Physiol. 85:529-536.
Woodson, W.R. and A.K. Handa (1987) Changes in protein patterns and in vivo protein synthesis during the development and senescence of Hibiscus petals. J. of Plant Physiol. 128:67-75.
Singh, N.K., P.C. LaRosa, A.K. Handa, P.M. Hasegawa and R.A. Bressan (1987) Hormonal regulation of protein synthesis associated with salt tolerance in plant cells. Proc. Natl. Acad. Sci., USA 84:739-743.
Marlow, S.J. and A. K. Handa (1987) Immuno slot blot assay using ImmunoAffinity membrane which covalently binds protein. J. Immunological Methods. 101:133-139.
Biggs, M.S., W.R. Woodson and A.K. Handa (1988) Biochemical basis of high temperature inhibition of ethylene biosynthesis in ripening tomato fruits. Physiol. Plant. 72:572-578.
Henstrand, J.M. and A.K. Handa (1989) Effect of ethylene action inhibitors upon wound-induced gene expression in tomato pericarp. Plant Physiol. 91:157-162.
Biggs, M.S. and A.K. Handa (1989) Temporal regulation of polygalacturonase gene expression in fruits from normal mutant and heterozygous tomato genotypes. Plant Physiol. 89:117-125.
Dyer, W.E., J.H. Henstrand, A.K. Handa, and K.M. Herrmann (1989) Wound induction of DAHP synthase in Solanaceae. Proc. Natl. Acad. Sci., USA 86:7370-7373.
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Tieman, D. and A.K. Handa (1989) Immunocytolocalization of polygalacturonase in ripening tomato fruit. Plant Physiol. 90:17-20.
Harriman, R.W., D.M. Tieman and A.K. Handa (1991) Molecular cloning of tomato pectin methylesterase gene and its expression in Rutgers, rin, nor and Nr tomato mutants. Plant Physiol. 97:80-87.
Jayaswal, R.K., A.K. Handa, R.A. Bressan, J. Cherniack, and P. Filner (1991) Physiological and heritable changes in cyclic AMP levels associated with changes in flagellar formation in Chlamydomonas reinhardtii (chlorophyta). J. of Phycology 27:587-591.
Henstrand, J.M., K.F. McCue, K. Brink, A.K. Handa, K.M. Herrmann and E.E. Conn (1992) Light and fungal elicitor induced 3-Deoxy-D-arabino-heptulosonate 7-phosphate synthase mRNA in suspension cultured cells of parsley (Petroselinum crispum L.). Plant Physiol. 98:761-763.
Tieman, D.M., R.W. Harriman, G. Ramamohan, and A.K. Handa (1992) An antisense pectin methylesterase gene alters pectin chemistry and soluble solids in tomato fruit. Plant Cell 4:667-679.
Görlach, J., A. Beck, J.M. Henstrand, A.K. Handa, K. Herrmann, J. Schmid and N. Amrhein (1993) Differential expression in tomato (Lycopersicon esculentum L.) of genes encoding shikimate pathways isoenzymes. I. 3-Deoxy-D-arabino-heptulosonate 7-phosphate synthase. Plant Mol. Biol. 23:697-706.
Gaffe, J., D.M. Tieman and A.K. Handa (1994) Pectin methyesterase isoforms in tomato (Lycopersicon esculentum) tissues: Effects of expression of a pectinmethylesterase antisense gene. Plant Physiol. 105:199-203.
Tieman, D.M. and A.K. Handa (1994) Reduction in pectin methylesterase activity modifies tissue integrity and cation levels in ripening tomato (Lycopersicon esculentum Mill.) fruits. Plant Physiol. 106:429-436.
Tieman, D.M. and A.K. Handa (1994) Regulation in pectin methylesterase activity modifies tissue integrity and cation levels in ripening tomato (Lycopersicon esculentum Mill.) fruits. Plant Physiol. 106:429-436.
Tieman, D.M., K.D. Kausch, D.M. Serra and A.K. Handa (1995) Field performance of transgenic tomato with reduced pectin methylesterase activity. J. Amer. Soc. Hort. Sci. 120:765-770.
Jones, J.D., J.M. Henstrand, A.K. Handa, K.M. Herrmann and S.C. Weller (1995) Impaired wound induction of 3-Deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase and altered stem development in transgenic potato plants expressing a DAHP-synthase antisense construct. Plant Physiol. 108:1413-1421.
Kagan-Zur, V., D.M. Tieman, S.J. Marlow, and A.K. Handa (1995) Differential regulation of polygalacturonase and pectin methylesterase gene expression during and after heat stress in ripening tomato (Lycopersicon esculentum Mill.) fruits. Plant Mol. Biol. 29:1101-1110.
Kausch, K.D. and A.K. Handa (1995) Molecular cloning and nucleotide sequence of a lipoxygenase cDNA from ripening tomato fruit. Plant Physiol. 107:669-670.
Thakur, B.R., R.K. Singh and A.K. Handa (1995) Effects of homogenization pressure on consistency of tomato juice. J. Food Quality 18:389-396.
Gaffe J, Tiznado ME, Handa AK (1996) Cloning and nucleotide sequence of a pectin methylesterase cDNA homologue (Accession No. U49330) from tomato leaves (PRG96-017). Plant Physiol. 110:1436.
Thakur, B.R., R.K. Singh, and A.K. Handa (1996) Effect of added soy protein on quality of tomato sauce. J. Food Processing 20:169-176.
Thakur, B.R., R.K. Singh, D.M. Tieman and A.K. Handa (1996) Quality of processed tomato products from transgenic tomato fruits with reduced levels of pectin methylesterase activity. J. Food Sci. 61:245-248.
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Thakur, B.R., Singh, R.K. and A.K. Handa (1996) Effects of an antisense pectin methylesterase gene on the chemistry of pectins in tomato (Lycopersicon esculentum) fruit juice. J. of Agr. Food Chem. 44:628-630.
Tieman, D.M. and A.K. Handa (1996) Molecular cloning and characterization of genes expressed during early tomato (Lycopersicon esculentum Mill.) fruit development by mRNA differential display. J. Amer. Soc. Hort. Sci. 121:52-56.
Turner, A.L., R.W. Harriman and A.K. Handa (1996) Isolation and nucleotide sequence of three tandemly arranged pectin methylesterase genes (Accession Nos. U70675, U70676 and U70677) from tomato (PGR 96-105). Plant Physiol 112:1397.
Turner, A.L., K.D. Kausch and A.K. Handa (1996) Isolation and nucleotide sequence of two cDNAs corresponding to tomato fruit pectin methylesterase genes (Genebank: U50985 and U50986) (PGR 96-035). Plant Physiol. 111:652.
Gaffe, J., M.E. Tiznado and A.K. Handa (1997) Characterization and functional expression of a ubiquitously expressed tomato pectin methylesterase. Plant Physiol. 114:1547-1556.
Frederick, R.D., J. Chiu, J.L. Bennetzen and A.K. Handa (1997) Identification of a pathogenicity locus, rpfA, in Erwinia carotovora subsp. Carotovora that encodes a two-component sensor-regulator protein. Mol. Plant Microbe Interactions. 10:407-415.
Thakur, B.R., R.K. Singh, and A.K. Handa (1997) Chemistry and uses of pectin - A review. Critical Reviews Food Science Nutrition 37:47-73.
Kausch, K.D. and A.K. Handa (1997) Molecular cloning of a ripening specific lipoxygenase and its expression during wild-type and mutant tomato fruit development. Plant Physiol. 113:1041-1050.
Frenkel, C., J.S. Peters, D.M. Tieman, M.E. Tiznado and A. K. Handa (1998) Pectin methylesterase regulates methanol and ethanol accumulation in ripening tomato (Lycopersiconesculentum) fruit. J. Biol. Chem. 273:4293-4295.
Mishra, K.K. and Handa, AK (1998) Post transcriptional silencing of pectin methylesterase gene in transgenic tomato fruits results from impaired pre-mRNA processing. Plant J. 14:583-592.
Chen, M.-H., J. Sheng, G. Hind, A.K. Handa, V. Citovsky (2000) Interaction between the tobacco mosaic virus movement protein and host cell: Pectin methylesterase is required for viral cell-to-cell movement. EMBO J. 19: 913-920.
Mattoo, A.K. and A.K. Handa (2001) Post-harvest science toward the third millenium. Acta Horticulturae 553:761-767.
Mehta, R.A., T. Cassol, N. Li, N. Ali, A.K. Handa and A.K. Mattoo (2002) Engineered polyamine accumulation in tomato enhances phytonutrient content, juice quality and vine life. Nature Biotech. 20:613-618.
Ramakrishna ,W., Z. Deng, C.-K. Ding, A.K. Handa and R.H. Ozminkowski, Jr. (2003) A novel small heat shock protein gene, vis1, contributes to pectin depolymerization and juice viscosity in tomato fruit. Plant Physiol. 131:725-735.
Tiznado-Hernández, M.E., J. Gaffe and A.K. Handa (2004) Isolation and study of a ubiquitously expressed tomato pectin methylesterase regulatory region. Electronic J. Biotech. 7:9-29. http://www.ejbiotechnology.info/content/vol7/issue1/full/1/index.html
Anjanasree K.N., A. Srivastava, A. Handa and K.C. Bansal (2005) Identification of differentially expressed ripening-related cDNA clones from tomato (Lycopersicon esculentum) using tomato EST array. Curr. Sci. 88:792-796
Mishra K.K. and A.K. Handa (2005) Meiotic reestablishment of post-transcriptional gene silencing is regulated by aberrant RNA formation in tomato (Lycopersicon esculentum cv. Mill.). J. Mol. Breeding 16:139-149.
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Srivastava, A. and A.K. Handa (2005) Hormonal regulation of fruit development: A Molecular perspective. J. of Plant Growth Regulation 24:67-82.
Mattoo A.K., A. Sobolev, R. Goyal, A.Neelam, A.K. Handa and A.L. Segre (2006) Metabolite profiles of transgenic tomato fruit engineered to accumulate polyamines spermidine and spermine using NMR spectroscopy reveal enhanced nitrogen-carbon interactions. Plant Physiol. 142:1759-1770; First published on October 13, 2006; 10.1104/pp.106.084400
Srivastava, A., S.H. Chung, T. Fatima, T. Datsenka, A.K. Handa, A.K. Mattoo (2007) Polyamines as anabolic growth regulators revealed by transcriptome analysis and metabolite profiles of tomato fruits engineered to accumulate spermidine and spermine. Plant Biotechnology 24: (In Press)
Neelam, A., T. Cassol, R.A. Mehta, A.A. Abdul-Baki, A.P. Sobolev, R.K. Goyal, J. Abbott, A.L. Segre, A.K. Handa, A.K. Mattoo (2007) Integration of transgenic tomato plants into an alternative agriculture production system illustrates a new paradigm for sustainable agriculture. Proc. Natl. Acad. Sci., U.S.A (In Press).
Book Chapters (total 21) Johri, M.M. and A.K. Handa (1976) Hormonal balance and cell differentiation in the protonema
of the moss Funaria hygrometrica. Proc. 9th International Conference on Plant Growth Substances at Lausanne (Ed P.E. Piret) pp. 168-170.
Larkins, B.A., K. Pedersen, W.J. Hurkman, A.K. Handa, A.C. Mason, C.Y. Tsai and M.A. Hermodson (1980) Maize storage proteins: Characterization and biosynthesis. In: Genome Organization and Expression in Plants, Plenum Press, New York. pp 203-217.
Handa, A.K., R.A. Bressan, S. Handa, and P.M. Hasegawa (1982) Tolerance to water and salt stress in cultured cells. In: Proceedings of the Vth International Congress of Plant Tissue and Cell Culture, Tokyo, Japan, July 11-16, 1982. pp. 471-474.
Bressan, R.A., N.K. Singh, A.K. Handa, A. Kononowicz and P.M. Hasegawa (1985) Stable and unstable tolerance to NaCl in cultured tobacco cells. In: Plant Genetics (a proceeding of the 1985 UCLA symposia) M. Freeling (Ed). Alan R. Liss, Inc. New York. pp. 755-769.
Handa, A.K., R.A. Bressan, A.G. Korty, R.K. Jayaswal and D.J. Charles (1987) Isolation and characterization of pectolytic nonpathogenic mutants of Erwinia carotovora subsp. carotovora. In: Plant Pathogenic Bacteria; Proc. VIth International Conference on Plant Pathogenic Bacteria. Martinus Nijhoff Publishers, Dordrecht. pp. 212-217.
Handa, A.K., R.A. Bressan, L. Lee, D.J. Charles, R.K. Jayaswal, L. Chiu and J.L. Bennetzen (1987) Characterization of pathogenicity genes of Erwinia carotovora. In: Molecular Genetics of Plant-Microbe Interactions. Proceedings of 3rd International Symposium of Molecular Genetics of Plant-Microbe Interactions. Martimus Nijhoff Publishers, Dordrect. pp. 67-72.
Bressan, R.A., N.K. Singh, A.K. Handa, R. Mount, J. Clithero, and P.M. Hasegawa (1987) Stability of altered genetic expression in cultured plant cells adapted to salt. In Drought Resistance in plants: genetic and physiological aspects. L. Monti and E. Porceddu, (Eds.) Commission of the European Communities, Brassels. pp. 41-57.
Bennetzen, J.L., S.H. Hulbert, P.J. San Miguel, C. Carter-Peek, J. Chiu and A.K. Handa (1988) Microbial Interactions with Maize. Genetics of Plant-Microbe Interactions. R. Palacios and D.P.S. Verma (Eds), APS Press, pp. 269-270.
Singh, N.K., D.E. Nelson, P.C. LaRosa, C.E. Bracker, A.K. Handa, P.M. Hasegawa and R.A. Bressan (1989) Osmotin, a protein associated with osmotic stress adaptation in plant cells.
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In: J. Cherry (ed.) Physiological and Biochemical Basis of Environmental Stress in Plants. Springer-Verlag. pp. 67-88.
Handa, A.K., J. Chiu, H. Rozycki and J.L. Bennetzen (1990) Evidence for global regulation of the expression of pathogenicity genes in soft-rot Erwiniae. In: Plant Pathogenic Bacteria; Proceedings of VIIth International Conference on Plant Pathogenic Bacteria. Z. Klement, Ed., Akademiai Kiado, Budapest, pp. 707-712.
Bressan, R.A. and A.K. Handa (1991) Molecular Genetics and Plant Physiologist Guest authors for Plant Physiol. 4th edition, F.B. Salisbury and C.W. Ross (Eds). Wadsworth Publishing Company, Belmont, CA. pp. 531-548.
Harriman, R.W., D.M. Tieman and A.K. Handa (1992) Localization of pectin methylesterase mRNA in tomato fruit. In: Tissue Printing, Tools for the Study of Anatomy, Histochemistry and Gene Expression. Eds. P.D. Reid, R.F. Pont-Lezica, E. Cambillo and R. Taylor. Academic Press, San Diego. pp. 121-123.
Tieman, D.M. and A.K. Handa (1992) Immunocytolocalization of polygalacturonase in ripening tomato fruit using a membrane that covalently binds protein. In: Tissue Printing, Tools for the Study of Anatomy, Histochemistry and Gene Expression. Eds. P.D. Reid, R.F. Pont-Lezica, E. Cambillo and R. Taylor. Academic Press, San Diego. pp. 47-57.
Handa, A.K., D.M. Tieman, K.K. Mishra, B.R. Thakur, and R.K. Singh (1996) Role of pectin methylesterase in tomato fruit ripening and quality attributes of processed tomato juice. In: Pectin and Pectinases; J. Visser, and A.G.J. Voragen, eds., Elsevier Science Publishers, Amsterdam . Progress in Biotechnology 14:355-366
Mehta, R., A. Handa and A. Mattoo (1998) Interactions of ethylene and polyamines in regulating fruit ripening. Biology and biotechnology of the plant hormone ethylene. Dordrecht ; Boston: Kluwer Academic Publishers, NATO ASI series. Partnership sub-series 3, High technology; vol. 34. pp. 321-326.
Mehta, R., A. Handa, and A.K Mattoo (1999) Ethylene, polyamines and fruit ripening. Plant Biotechnology and In Vitro Biology in the 21st Century. Kluwver Acad. Publ. Boston. pp. 591-595.
Mehta, R.A., D. Zhou, M. Tucker, A. Handa, T. Solomos and A.K. Mattoo (1999) Ethylene in higher plants: biosynthetic interactions with polyamines and high-temperature-mediated differential induction of Nr versus TAE1 ethylene receptor. In: Biology and Biotechnology of the Plant Hormone Ethylene II (A.K. Kanellis et al., eds.), Kluwer Acad. Publ., Boston. pp. 387-393.
Mattoo, A.K., T. Cassol, R. Mehta, N. Ali, A. Abdul-Baki and A. Handa (2002) Genetic engineering of tomato fruit for sustained accumulation of polyamines during ripening to study their physiological role(s). Proc. IS on Trop. Subtrop Fruits, Cairns, Australia (R. Drew, ed.). Acta Hort. 575 (Vol. 1): 157-161. 2002.
Mattoo, A.K. A.K. Handa (2003) Ethylene signalling in plant cell death. In: Plant Cell Death Processes. Ed. Nooden, L.D. Elesevier and Academic Press. San Diego and London, pgs. 125-142.
Mattoo, A.K., T. Cassol, R.A. Mehta, R. Goyal, A. Neelam, A.S.-H. Chung, V. Kumar, A.K. Handa (2003) Lessons one can learn from studying transgenic tomatoes that accumulate higher polyamines in a ripening-specific manner. In: Biology and Biotechnology of the Plant Hormone Ethylene III. Eds. M. Vendrell, H. Klee, J-C. Pech, and F. Romojaro, IOS Press. Amsterdam, Pgs. 359-364.
Handa, A.K., A. Srivastava and V. Perla (2007) Hormonal control of fruit ripening. In: Genetic Improvement of Solanaceous Crops. Razdan, M.K. and Mattoo, A.K (Eds) Science Publishers Inc, Enfield, New Hampshire, USA, pp 313-342.
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Contributed papers at National and international conferences and meetings:
(A total of about 145) Handa, A. K., J. Cherniack, and P. Filner. 1977. Release of flagellar proteins from
Chlamydomonas reinhardtii during flagellar shortening caused by pyrophosphate. J. Cell Biology 77, 285a
Bressan, R. A., H. Quader, A. K. Handa, and P. Filner. 1978. Cyclic AMP in Ochromonas. Plant Physiology 61, 30a.
Handa, A. K., and R. A. Bressan. 1978. A new assay for adenosine 3',5', cyclic monophosphate. Plant Physiology 61, 101a.
Handa, A. K., R. A. Bressan, J. Cherniack, and P. Filner. 1979. Physiologically and genetically determined changes in Cyclic AMP level associated with changes in flagellar formation in Chlamydomonas reinhardtii. Plant Physiology 63, 145a.
Handa, A. K., M. Hermodson, C. Y. Tsai, and B. A. Larkins. 1979. Analysis of isoelectric hetrogeneity among zein proteins. Plant Physiology. 63, 95a.
Kulkarni, P., A. K. Handa, R. A. Bressan, and P. M. Hasegawa 1980. Regulation of sodium polypectate induced endopoly- glacturonate transeliminase in E. carotovora. HortScience 14(6).
Bressan, R. A., P. M. Hasegawa, and A. K. Handa. 1980. Resistance to water and salt stress of plant cells grown in vitro. In Vitro 16, 242.
Handa, A. K., R. A. Bressan, and P. M. Hasegawa. 1980. Populations of cultured higher plant cells resistant to salt. In Vitro 16, 231-232.
Handa, A. K., P. M. Hasegawa, and R. A. Bressan. 1981. The use of plant cell cultures to study host-pathogen interactions. In Vitro 17, 231.
Handa, A. K., R. A. Bressan, and P. M. Hasegawa. 1981. Changes in cultured tomato cells exposed to low water potentials. Plant Physiology 67, 122a.
Jayaswal, R. K., A. K. Handa, R. A. Bressan, and P. M. Hasegawa. 1981. Regulation of toxin synthesis by Alternaria solani. Plant Physiology 67, 108a.
Kulkarni, P., A. K. Handa, R. A. Bressan, and P. M. Hasegawa. 1981. Regulation of sodium polypectate induced endopoly- galacturonate transeliminase in Erwinia carotovora. Plant Physiology 67, 14a.
Elhag, H. M., P. M. Hasegawa, R. A. Bressan, and A. K. Handa 1981. Resistance of salt stress among clonal lines of N. tabacum. ASHS Annual Meeting.
Lopez-Peralta, C., P. M. Hasegawa, R. A. Bressan, and A. K. Handa. 1981. Comparison of tolerances to polyethylene glycol induced water stress between two tomato genotypes. ASHS Annual Meeting.
Handa, S., R. A. Bressan, P. M. Hasegawa, and A. K. Handa. 1982. Relationship between proline accmulation and osmotic adjustment in cultured plant cells. ASPP Annual Meeting. Plant Physiology 69:59a.
Kulkarni, P., A. K. Handa, R. A. Bressan, and P. M. Hasegawa. 1982. Cellular mechanisms underlying the pathogenicity of Erwinia carotovora. ASSP Annual Meeting. Plant Physiology 69:144a.
Handa, S., R. A. Bressan, A. K. Handa, N. C. Carpita, and P. M. Hasegawa. 1982. Osmotic adaptation in cultured plant cells. ASPP Annual Meeting. Plant Physiology 69:149a.
Hasegawa, P. M., R. A. Bressan and A. K. Handa. 1983. Salt tolerance in cultured plant cells. 34th Annual Meeting of the Tissue Culture Association.
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Rhodes, D., A. Myers, G. Jamieson, S. Handa, R. A. Bressan, P. M. Hasegawa and A. K. Handa. 1983. Nitrogen metabolism of cultured tomato cells adapted to water stress. Plant Physiology 72, 93a.
Singh, N. K., A. K. Handa, P. M. Hasegawa and R. A. Bressan. 1983. Electrophoretic protein patterns in cultured cells of tobacco adapted to NaCl. Plant Physiology 72, 94a.
Biggs, M. S. and A. K. Handa. 1983. Partial protection of polygalacturonase from tomato fruit against heat inactivation by polygalacturonic acid. Plant Physiology 72, 125a.
Binzel, M. L., P. M. Hasegawa, R. A. Bressan, S. Handa and A. K. Handa. 1983. Adaptive responses of cultured tobacco cells to NaCl. Plant Physiology 72, 136a.
Hasegawa, P. M., R. A. Bressan and A. K. Handa. 1983. Tolerance to water stress in higher plant cells. 80th Annual Meeting of ASHS.
Handa, A. K., M. S. Biggs, N. K. Singh and R. A. Bressan. 1983. Effect of tunicamycin on ripening of tomato pericarp discs. 80th Annual Meeting of ASHS.
Handa, A. K., N. K. Singh and M. S. Biggs. 1983. Electrophoretic protein patterns of tomato fruit in normal and non-ripening mutants during ripening. 80th Annual Meeting of ASHS.
Jayaswal, R. K., R. A. Bressan and A. K. Handa. 1984. Mu mutagenesis of phytopathogenic Erwinia carotovora. 84th Annual Meeting of American Society for Microbiology, March 984.
Hasegawa, P. M., R. A. Bressan and A. K. Handa. 1984. Utilizing cell cultures to study cellular mechanisms of salinity and water stress tolerance in plants. American Association for the Advancement of Science. May 1984.
Singh, N. K., P. C. LaRosa, R. A. Bressan, P. M. Hasegawa, and A. K. Handa. 1984. Protein synthesis in response to NaCl adaptation and abscisic acid. 1984 UCLA Symposia on Cellular and Molecular Biology of Plant Stress. April 1984.
Singh, N. K., A. K. Handa, P. M. Hasegawa and R. A. Bressan. 1984. Expression of salt tolerance specific proteins in cultured tobacco cells. 35th Annual Meeting of the Tissue Culture Association. June 1984.
Biggs, M. S., D. M. Tieman and A. K. Handa. 1984. Polygalac- turonic acid hydrolysis by tomato fruit polygalacturonase isozymes. 81st Annual Meeting of ASHS. August 1984.
Handa, A. K., R. A. Bressan and A. Korty. 1984. Isolation and characterization of Mu d1-insertion induced nonpathogenic mutants of Erwinia carotovora subsp. carotovora. Plant Physiol. 75s 166. Annual Meeting of ASPP. August 1984.
Handa, A. K. 1984. Study of genetic determinants responsible for the pathogenicity of Erwinia carotovora subsp. carotovora. Molecular basis of Plant Disease Conference. Davis, California. August 19-23, 1984.
Jayaswal, R. K., R. A. Bressan, A. G. Korty, and A. K. Handa. 1984. Use of Mu d1 (Apr lac cts62) induced mutagenesis to study the regulation of pathogenicity of Erwinia carotovora subsp. carotovora. Molecular Form and Function of the Plant Genome, NATO Advanced Studies Institute, Renesse, The Netherlands, July 4-14, 1984.
Handa, A. K. 1984. Basis of pathogenicity of Erwinia carotovora subsp. carotovora. Second International Symposium, The Molecular Genetics of the Bacteria - Plant Interaction. Ithaca, New York, June 4-8, 1984.
Handa, A. K., N. K. Singh, R. A. Bressan and P. M. Hasegawa. 1984. Expression of salt tolerance specific proteins in cultured tobacco cells. Gordon Research Conference on Plant Molecular Biology. June 11-16, 1984.
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Hasegawa, P. M., R. A. Bressan and A. K. Handa. 1984. Mechanisms of salinity tolerance in isolated plant cells. Annual Meeting of Midwest Tissue Culture Association, September 12, 1984.
Jayaswal, R. K., R. A. Bressan and A. K. Handa. Mu d1 (Apr lac cts62) induced mutagenesis to study the genetics of pathogenicity of Erwinia carotovora subsp. carotovora. EMBO workshop on Soft rot Erwiniae, Marseille-Luxing, July 23-27, 1984.
Handa, S., R. A. Bressan, A. K. Handa, and P. M. Hasegawa. 1984. Compartmentation of proline in water and salt stress adapted cells. Plant Physiol. 75s, 65. Annual Meeting of ASPP, August 1984.
Singh, N. K., A. K. Handa, P. M. Hasegawa, and R. A. Bressan. 1984. Synthesis of A 26 Kd protein in response to NaCl stress and abscisic acid. Plant Physiol. 75s, 65. Annual Meeting of ASPP. August 1984.
Handa, A.K. 1985. Isolation and characterization of pectolytic nonpathogenic mutants of Erwinia carotovora subsp. carotovora. The VI International Conference on Plant Pathogenic Bacteria. College Park, U.S.A. June 1985.
Biggs, M.S., R.W. Harriman, J.H. Cherry and A.K. Handa. 1985. Changes in mRNA populations during tomato fruit development. Annual meeting of ASPP. June 1985.
Singh, N.K., P.M. Hasegawa, R.A. Bressan, A.K. Handa and M.A. Hermodson. 1985. Characterization of 26 kilodalton protein associated with NaCl and water stress adaptation. Annual Meeting of ASPP. June 1985.
Tieman, D.M., M.S. Biggs and A.K. Handa. 1985. Biochemical characterization of tomato fruit polygalacturonase isozymes. Annual Meeting of ASHS. August 1985.
Biggs, M.S., D.M. Tieman and A.K. Handa. 1985. Ripening associated gene expression in tomato fruit pericarp. Annual Meeting of ASHS. August 1985.
Marlow, S.J., M.S. Biggs and A.K. Handa. 1985. Enzyme linked immunosorbent assay (ELISA) for tomato polygalacturonases. Annual Meeting of ASHS. August 1985.
Biggs, M.S., R.W. Harriman and A.K. Handa. 1985. Changes in gene expression during tomato fruit ripening. 13th International Congress of Biochemistry. Amsterdam, The Netherlands. August 1985.
Singh, N.K., R.A. Bressan, P.M. Hasegawa, and A.K. Handa. 1985. Abscisic acid induced proteins and NaCl adaptation in cultured plant cells. 1st International Congress of Plant Molecular Biology, Savannah, GA. October 1985.
Biggs, M.S., R.W. Harriman, D.M. Tieman, and A.K. Handa. 1985. Developmentally regulated changes in gene expression during tomato fruit ripening. 1st International Congress of Plant Molecular Biology. Savannah, GA. October 1985.
Singh, N.K., P.M. Hasegawa, A.K. Handa, and R.A. Bressan. 1986. Post-transcriptional events regulate expressional changes in NaCl adapted cells. Annual Meeting of ASPP. June 1986.
Woodson, W.R. and A.K. Handa. 1986. Changes in protein patterns and in vivo protein synthesis during senescence of hibiscus petals. Annual Meeting of ASPP. June 1986.
Handa, A.K., L. Lisa, J. Chiu, D.J. Charles, and J.L. Bennetzen. 1986. Characterization of pathogenicity genes of Erwinia carotovora. Third International Symposium on the Molecular Genetics of Plant-Microbe Interactions. Montreal, Canada. July 1986.
Handa, A.K., W.R. Woodson, and M.S. Biggs. 1986. Biochemical basis of high temperature stress-induced inhibition of ethylene biosynthesis in ripening tomato fruits. Tomato Biotechnology Symposium. Davis, CA. August 1986.
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Harriman, R.W., D.M. Tieman, M.S. Biggs, J.H. Cherry and A.K. Handa. 1986. Polypeptide characterization of tomato PG1 and its in vitro conversion to PG2. Tomato Bioechnology Symposium. Davis, CA. August 1986.
Marlow, J. and A.K. Handa. 1986. Changes in levels of polygalacturonase activity and protein during tomato fruit ripening. Tomato Biotechnology Symposium. Davis, CA. August 1986.
Biggs, M.S., D.M. Tieman and A.K. Handa. 1987. Quantification of polygalacturonase mRNA levels in tomato fruit ripening mutants and their heterozygotes with the normal Rutger genotype. Annual Meeting of ASPP. July 1987.
Biggs, M.S., W.H. Flurkey and A.K. Handa. 1987. Peroxidase gene expression during tomato fruit ripening. Annual Meeting of ASPP. July 1987.
Hendstrand, J.M. and A.K. Handa. 1987. Wound-induced changes in mRNA populations in tomato pericarp tissue. Annual Meeting of ASPP. July 1987.
Handa, A.K., H. Rozycki, J. Chiu, D.J. Charles and J.L. Bennetzen. 1987. Characterization of pathogenicity caused by Erwinis carotovora subsp. carotovora and its Mud1-induced mutants. Fallen Leaf Lake Conference on the genus Erwinia. September 17-20, 1987.
Rozicki, H., J.L. Bennetzen and A.K. Handa. 1987. Characterization of the effects of oxygen tension on Erwinia carotovora subsp. carotovora. Fallen Leaf Lake Conference on thegenus Erwinia. September 17-20, 1987.
Chiu, J., J.L. Bennetzen and A.K. Handa. 1987. Molecular cloning of a virulence gene from Erwinia carotovora subsp. carotovora. Fallen Leaf Lake Conference on the genus Erwinia. September 17-20, 1987.
Biggs, M.S., W.H. Flurkey and A.K. Handa. 1987. Expression of a peroxidase gene during tomato fruit ripening. Annual Meeting of ASHS, November 6-12, 1987.
Biggs, M.S., D.M. Tieman and A.K. Handa. 1987. Changes in the expression of polygalacturonase mRNA in normal, mutants and heterozygous tomato fruits during development and detection of the PG gene in other Lycopersicon species. Annual Meeting of ASHS, November 6-12, 1987.
Marlow, S.J. and A.K. Handa. 1987. Characterization of heat stress effect on polygalacturonase synthesis during tomato fruit ripening. Annual Meeting of ASHS, November 6-12, 1987.
Dyer, W.E., J.H. Henstrand, A.K. Handa, S.C Weller, and K.M. Herrmann. 1988. Wound induced 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase in solanaceae. Annual Meeting of ASPP, Reno, Nevada, July 10-14, 1988.
Tieman, D.M., M.S. Biggs, D.N. Kuhn, and A.K. Handa. 1988. Ripening associated changes in hydroxyproline rich glycoproteins (HRGP) mRNAs accumulation in tomato fruit. Annual Meeting of ASPP, Reno, Nevada, July 10-14, 1988.
Henstrand, J.M., D.M. Tieman, W.E. Dyer, K.M. Herrmann, and A.K. Handa. 1988. Developmental and wound induced expression of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase in tomato pericarp tissue. Annual Meeting of ASPP, Reno, Nevada, July 10-14, 1988.
Harriman, R.W., J.H. Cherry and A.K. Handa. 1988. Purification and characterization of tomato fruit pectinmethyl esterase. Annual Meeting of ASPP, Reno, Nevada, July 10-14, 1988.
Tieman, D.M., M.S. Biggs, D.N. Kuhn, and Avtar K. Handa. 1988. Changes in hydroxyproline rich glycoproteins (HRGPs) mRNAs accumulation during tomato fruit ripening. Annual Meeting of ASHS, East Lansing, MI, August 6-11, 1988.
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Tieman, D.M., S.J. Marlow and A.K. Handa. 1988. Immunolocalization of polygalacturonase in ripening tomato fruits. Annual Meeting of ASHS, E. Lansing, MI, August 6-11, 1988.
Harriman, R.W., J.H. Cherry, and A.K. Handa. 1988. Changes in expression of pectinmethyl esterase in developing tomato fruit. Annual Meeting of ASHS, E. Lansing, MI, August 6-11, 1988.
Hendstrand, J.M., and A.K. Handa. 1988. The effect of ethylene action inhibitors upon wound-induced mRNA species in immature tomato pericarp tissue. Annual Meeting of ASHS, E. Lansing, MI, August 6-11, 1988.
Chiu, J., J.L. Bennetzen and A.K. Handa. 1989. Molecular cloning of DNA sequences which regulate production of extracellular enzymes in Soft rot Erwinias. Annual Meeting of American Society for Microbiology. New Orleans, LA, May 14-18, 1989.
Henstrand, J.M., W.E. Dyer, K.M. Herrmann, and A.K. Handa. 1989. Molecular cloning, characterization and wound induction of tomato 3-Deoxy-D-arabino-heptulosonate-7-phosphate synthase. UCLA symposium on Plant Gene Transfer. Park City, UT, April 1-7, 1989.
Handa, A.K., J. Chiu, H. Rozycki, and J.L. Bennetzen. 1989. Evidence for global regulation of the expression of pathogenicity genes in soft rot Erwinias. 7th International Conference on Plant Pathogenic Bacteria, Budapest, Hungary. June 11-17, 1989.
Nelson, D.E., D.M. Tieman, R.W. Harriman, N.K. Singh, A.K. Handa, and R.A. Bressan. 1989. Expression of Osmotin in ripening tomato fruit. Annual Meeting of ASPP. 1989.
Harriman, R.W., D.M. Tieman and A.K. Handa. 1989. Localization of PME and PG mRNAs and proteins in developing tomato fruit. Horticultural Biotechnology Symposium. Univnersity of California, Davis. August 21-23, 1989.
Henstrand, J.M., K.M. Herrmann and A.K. Handa. 1990. Molecular cloning, characterization and wound induction of tomato 3-Deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase. Annual Meeting of ASPP 1990, Indianapolis.
Tieman, D.M., R.W. Harriman and A.K. Handa. 1990. Accumulation and localization of pectin methylesterase and polygalacturonase mRNAs in developing tomato fruit. Annual Meeting of ASPP 1990, Indianapolis.
Harriman, R.W. and A.K. Handa. 1990. Identification and characterization of three pectin methylesterase genes in tomato. Annual Meeting of ASPP 1990, Indianapolis.
Handa, A.K., R.W. Harriman and D.M. Tieman. 1990. Tomato pectinmethylesterase: mRNA localization and analysis of genomic clones. Gordon Conference on Postharvest Physiology. Andover, NH, July 9-13, 1990.
Henstrand, J.M., J.E.B.P. Pinto, K.W. Herrmann, and A.K. Handa. 1991. Expression of a wound-inducible promoter in transgenic potato. UCLA Symposia and Colloquia on The Genetic Dissection of Plant Cell Processes. Keystone, CO, January 1991.
Tieman, D.M., G. Ramamohan, R.W. Harriman and A.K. Handa. 1991. Inhibition of pectin methylesterase gene expression in transgenic tomato fruit by anti-sense RNA. UCLA Symposia and Colloquia on the Genetic Dissection of Plant Cell Processes. Keystone, CO, January 1991.
Kagan-Zur, V., D.M. Tieman, Y. Mizrahi and A.K. Handa. 1991. Polygalacturonase expression is impaired by heat storage of ripening tomato fruits. 3rd International Congress of Plant Molecular BIology. Tucson, AZ, October 1991.
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Tieman, D.M., R.W. Harriman, G. Ramamohan and A.K. Handa. 1991. Antisense RNA inhibition of pectinmethylesterase expression in transgenic tomatoes. 3rd International Congress of Plant Molecular BIology. Tucson, AZ, October 1991.
Herrmann, K.M., A.K. Handa and J.M. Henstrand. 1991. Aromatic secondary products regulate carbon flow into the shikimate pathway. 3rd International Congress of Plant Molecular BIology. Tucson, AZ, October 1991.
Henstrand, J.M., J.E.B.P. Pinto, L.M. Weaver, K.M. Herrmann and A.K. Handa. 1991. Preliminary characterization of the promoter for the first enzyme of the shikimate pathway in potato. 3rd International Congress of Plant Molecular Biology, Tucson, AZ, October 1991.
Tieman, D.M. and A.K. Handa. 1992. Role of pectinmethylesterase in tomato fruit development. 1992. Gordon Research Conference on Plant Senescence. Plymouth State College. July 1992.
Tieman, D.M., D.M. Serra, A.K. Handa. Effects of polygalacturonase gene expression on growth and development of transgenic tomato plants. Annual Meeting of American Society of Plant Physiologists. Pittsburgh. August 1992.
Tieman, D.M., K.D. Kausch, D.M. Serra, A.K. Handa. Characterization of transgenic tomato fruits with lower levels of pectin methylesterase. Annual Meeting of American Society of Plant Physiologists. Pittsburgh. August 1992.
Rahmatullah, R.J., N.K. Singh, S. Tuzun, A.K. Handa, C.M. Peterson. Induction of genes involved in cell wall degradation and hypersensitive response in soybean abscission zones. Annual Meeting of Plant Physiologists. Pittsburgh. August 1992.
Tieman, D.M., Z. Ristic, E. Ashworth, A.K. Handa. Expression of a tomato polygalacturonase gene in tomato affects plant growth and development. Gordon Research Conference on Plant Senescence. July 1992.
Rahmatullah, R.J., N.K. Singh, S. Tuzun, A.K. Handa, C.M. Peterson. Induction of genes involved in cell wall degradation and hypersensitive response in soybean abscission zones. Gordon Research Conference on Plant Senescence. July 1992.
Tieman, D.M., A.K. Handa. Characterization of transgenic tomato fruits expressing an antisense pectin methylesterase gene. Symposium on the Molecular Biology of Tomato: Fundamental Advances and Crop Impovement. Davis, California. August 1992.
Kagan-Zur, V., P. Parker, A.K. Handa. Heat storage of ripening tomato fruits impairs polygalacturonase expression more acutely than that of pectin methylesterase. Symposium on the Molecular Biology of Tomato: Fundamental Advances and Crop Improvement. Davis, California, August 1992.
Gaffe, J., D.M. Tieman, A.K. Handa. Identification of pectinmethylesterase isoforms from fruit and vegetative tissue of wild type and transgenic tomatoes. Annual Meeting of American Society of Plant Physiologists. Minneapolis. July 1993.
Tieman, D.M., D.M. Serra, K.D. Kausch, A.K. Handa. Effects of expression of an antisense pectin methylesterase RNA on field performance of transgenic tomatoes. Annual Meeting of American Society of Plant Physiologists. Minneapolis. July 1993.
Jones, J.D., J.M. Henstrand, A.K. Handa, S.C. Weller. Reduced wound induction of DAHP synthase due to expression of antisense DAHP synthase RNA in potato. Annual Meeting of American Society of Plant Physiologists. Minneapolis. July 1993.
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Kausch, K.D., A.K. Handa. Molecular Cloning and identification of an 8-kD protein from ripening tomato pericarp as a lipoxygenase. Annual Meeting of American Society of Plant Physiologists. Minneapolis. July 1993.
Tieman, D.M., K.D. Kausch, D.M. Serra, A.K. Handa. Field performance of transgenic tomatoes with reduced pectin methylesterase activity.l Annual Meeting of American Society of Horticultural Science. Nashville, TN. July 1993.
Gaffe, J., D.M. Tieman and A.K. Handa. Cloning and characterization of a pectin methylesterase expressed throughout growth and developmcnt of tomato plants. Annual Meeting of American Society of Plant Physiologists. Portland, OR. July 1994.
Mishra, K.K. and A.K. Handa. Coordinate and tissue specific cosilencing of a fruit PME gene and a homologous transgene in tomato fruit is associated with endogenous gene expression and an increase in RNA turnover. Annual Meeting of American Society of Plant Physiologists. Charlotte, North Carolina. July 1995.
Kausch, K.D. and A.K. Handa. Characterization of lipoxygenase expression in ripening tomato fruit. Annual Meeting of American Society of Plant Physiologists. Charlotte, North Carolina. July 1995.
Tieman, D.M. and A.K. Handa. Molecular cloning and characterization of genes expressed during early tomato (Lycopersicon esculentum Mill.) fruit development by mRNA differential display. Annual Meeting of American Society of Plant Physiologists. Charlotte, North Carolina. July 1995.
Tiznado, M.E., J. Gaffe, and A.K. Handa. Purification and characterization of a PME isozyme present in transgenic tomato fruit expressing a fruit PME antisense gene. Annual Meeting of American Society of Plant Physiologists. Charlotte, North Carolina. July 1995.
Gaffe, J., M.E. Tiznado and A.K. Handa. Cloning and characterization of a pectin methylesterase gene expressed throughout growth and development of tomato plant. Annual Meeting of American Society of Plant Physiologists. Charlotte, North Carolina. July 1995.
Singh, R.K., B.R. Thakur, and A.K. Handa. Quality characterization of processed products from genetically modified tomato fruits. Annual Meeting of American Institute of Chemical Engineers.
Handa, A.K. and K.D. Kausch (1996) Evaluation of role of a ripening specific lipoxygenase in fruit ripening using transgenic tomato fruits. Gordon Research Conference on Plant Senesence and Programmed Cell Death. Plymouth State College, New Hampshire. July 14-19.
Gaffe, J., Mishra, K.K., Tiznado M.E. and. Handa A.K. (1997) Functional expression of a ubiquitously expressed tomato pectin methylesterase gene in transgenic tobacco plants. Annual ASPP meeting.
Tiznado, M.E., Gaffe, J. and Handa, A. K. (1997) Analysis of a ubiquitously expressed pectin methylesterase promoter in transgenic tobacco. Annual ASPP meeting.
Mishra, K. K. and Handa, A. K. (1997) Homology dependent gene silencing in transgenic tomato fruits expressing a truncated pectin methylesterase gene is associated with pre-mRNA processing Annual ASPP meeting.
Tiznado, M., Gaffe, J. and Handa A.K. (1998) Over Expression of Pectin Methylesterase Does not Alter Cell Expansion in Transgenic Tobacco Cell Lines. 1998 Congress on In Vitro Biology, May 30-June4, Las Vegas, Nevada.
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Mishra, K.K. and A.K. Handa (1998) Gene dosage and genetic imprinting play roles in cosuppression phenomenon. Annual ASPP meeting, June 27-July 1, Madison, Wisconsin..
Handa A.K., Mishra K.K., Deng Z., Tiznado, M.E. and Gaffe J. (1998). Over expression of a tomato pectin methylesterase gene enhances plant growth but impairs seed set in transgenic tobacco plants. Annual ASPP meeting, June 27-July 1, Madison, Wisconsin,
Tiznado, M.E., Gaffe, J. and Handa A.K. (1998) Expression of the Tomato pmeu1 Promoter in homologous Tomato and Heterologous Tobacco Plant Species is Similar. Annual ASPP meeting, June 27-July 1, Madison, Wisconsin.
Mehta R., Cassol T., Ali N., Handa A. and Mattoo A. (1998) Polyamines and ethylene interactions in transgenic tomato fruit engineered to express a key polyamine-pathway gene during ripening. Biology and Bio-technology of the Plant Hormone Ethylene, Santorini Greece.
Ramakrishna W., Deng Z., Handa A. K. (1999) Functional genomics of tomato fruit quality attributes. Annual ASPP meeting, July 24 - July 28, 1999, Baltimore, MD
Sastry J. G., Deng Z., Mishra K. K., Tiznado M. E., Gaffe J., Kudla K. L. and Handa A. K. (1999) Characterization of effects of over-expression of a tomato pectin methylesterase (pmeu1, accession no. U49330) on seed set in transgenic tobacco plants. Annual ASPP meeting, July 24 - July 28, 1999, Baltimore, MD
Deng Z., Ramakrishna W., Handa A. K. (1999) Molecular characterization and genomic organization of a gene (designated bot 1) that regulates consistency of tomato fruit juice. Annual ASPP meeting, July 24 - July 28, 1999, Baltimore, MD
Autar Mattoo, Tatiana Cassol, Avtar K. Handa, (1999) Tomato fruit transformed with the yeast SAMdc gene accumulate spermine and lycopene. Annual ASPP meeting, July 24 - July 28, 1999, Baltimore, MD
Tatiana Cassol, Roshni Mehta, Nasreen Ali, Avtar K. Handa, Autar Mattoo (2000) Molecular analysis of tomato fruit engineered to accumulate polyamines during ripening. Plant Biology 2000 - July 15 - July 19, 2000, San Diego, CA.
Chang-Kui Ding, Zhiping Deng and Avtar K. Handa (2000) Functional characterization of bot1, a sHSP whose expression is associated with the tomato juice thickness. Plant Biology 2000 - July 15 - July 19, 2000, San Diego, CA.
Wusirika Ramakrishna, Jerson R. Dominguez, Avtar K. Handa (2000) Differential expression and promoter analysis of fruit specific tomato pectin methylesterase genes. Plant Biology 2000 - July 15 - July 19, 2000, San Diego, CA.
Jayanty G. Sastry, Mathew Jenks, and Avtar K. Handa (2000) Identification and characterization of changes of cuticular waxes in different genotypes of tomato during fruit ripening. Plant Biology 2000 - July 15 - July 19, 2000, San Diego, CA.
Jayanty G. Sastry, Bruce A Watkins, Mathew Jenks and Avtar K. Handa (2000) Role of lipoxygenase in lipid metabolism during tomato fruit ripening. Plant Biology 2000 - July 15 - July 19, 2000, San Diego, CA.
Deng Z., Handa A. K ( 2001) Role of Heat Shock Proteins in Tomato Fruit Ripening. ASPP 2001 Annual Meeting, July 21 - 25, 2001, Providence, RI
Dominguez JR, Bressan RA, Hasegawa PM and Handa AK (2001) Phenotypic characterization of transposon tagged tomato mutants. ASPP 2001 Annual Meeting, July 21 - 25, 2001, Providence, RI
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Deng Z., Handa A. K ( 2001) Effects of diallelic polymorphism of vis1 on fruit quality and field performance of tomato genotypes. The Annual meeting ASHS-2001, July 22-25, 2001, Sacramento, CA.
Mishra, K. K. and A. K. Handa. (2002) Meiotic Reestablishment of post-transcriptional gene silencing is associated with aberrant RNA formation. Self-Perpetuating Structural States in Biology, Disease, and Genetics. The Arthur M. Sackler Colloquia of The National Academy of Sciences Gene silencing. Washington, D.C.. March 22-24, 2002
Dominguez, J. R., R. A. Bressan and A. K. Handa. (2002) Characterization of germinal transposition of Tag1 in MicroTom Tomatoes. ASPP 2002 Annual Meeting. Denver, CO. August 3-7, 2002.
Deng, Z. and A. K. Handa. (2002) Fruit ripening regulates synthesis of HSPs. ASPP 2002 Annual Meeting . Denver, CO. August, 2002
Handa A. K. and Z. Deng (2003) Genetically altered expression of vis1, a small heat shock protein, modifies fruit quality attributes in transgenic tomato. The Annual Meeting of American society of Plant Biologists. Honolulu, Hawaii. July 2003
Dominguez, J. R. and A. K. Handa. (2003). Temporal and spatial expression of tomato fruit pectin methylesterase promoters in homologous and heterologous species. Plant Biology 2003. The Annual Meeting of American society of Plant Biologists. Honolulu, Hawaii. July 2003
Neelam, A., A. K. Handa and A. K. Mattoo. (2003) High levels of polyamines in transgenic tomato fruit affect ethylene biosynthesis and its signal transduction pathway during ripening . The Annual Meeting of American society of Plant Biologists. Honolulu, Hawaii. July 2003
Mattoo, A.K., A. Neelam. R. Goyal, T. Solomos and A. K. Handa (2003) High levels of polyamines in transgenic tomato fruit affect ethylene biosynthesis and its signal transduction pathway during ripening. Plant Biology 2003. The Annual Meeting of American society of Plant Biologists. Honolulu, Hawaii. July 2003
Goyal, R. , A. Bernadac, N. Ali, A. K. Handa and A.K. Mattoo. July 2003. Tomato pathogenesis related protein 1b1 (PrP1b1): specific induction upon chilling and sustained accumulation in transgenic fruit expressing yeast S-adenosylmethionine decarboxylase. The Annual Meeting of American society of Plant Biologists. Honolulu, Hawaii. July 2003
Handa, A. K., A. K. Mattoo and V. Perla (2003). Field performance of genetically engineered high lycopene tomatoes. The Annual Meeting of American Society of Chemists. New Orleans, Louisiana, March 23, 2003.
Faltin, Z., A. Perl, M. Velcheva, M Tsapovetsky, D. Holland, P. Roeckel-Drevet, A.K. Handa, M. Abu-Abied, M. Friedman-Einat and Y Eshdat (2004) Enhanced catalytic activity of phospholipid hydroperoxide glutathione peroxidase interferes with plant regeneration Plant Biology 2004, Lake Buena Vista, FL USA
Negi, P., A.S. Murphy and A. K. Handa, (2004) Expression of carotenoid biosynthesis pathway genes during ripening in bell pepper (Capsicum annuum l.) fruit. 2004 AAAS Annual Meeting, Seattle, WA, February 12-16, 2004
Negi P.S., Z. Deng and A. K. Handa (2004) A novel heat shock protein delays senescence and maintains the membrane integrity in tomato. Gordon Research Conference on Plant senescence. South Hadley, MA June27- July 2, 2004
Mattoo A. K. and A. K. Handa . (2005). Do higher polyamines regulate gene expression via cross talk with other hormones? Plant biology 2005, Seattle, Washington
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Mattoo A., A. Srivastava, S. Chung, A. Sobolevc, A. Neelam, R. Goyal, T. Datsenka, A. K. Handa, C. Segre. 2005. Metabolite-profiling and transcriptome analyses of transgenic tomato fruit reveal role of spermidine/spermine in C signaling and anabolic metabolism. 2nd Solanaceae Genome Workshop, September 25 – 29, 2005, Ischia, ITALY
Handa A.K., A. K. Mattoo, A. Srivastava, T. Datsenka (2005) Polyamines regulated transcription in ripening tomato fruits. Eucarpia Tomato 2005, XV Meeting of the Eucarpia Tomato Working Group, Bari, Italy
Handa A.K., A. Srivastava, T. Datsenka and L McIntyre (2006) Transcriptional regulation during tomato fruit development: Identification of novel genes involved in fruit maturation and ripening. VI Iinternational Solanacae Conference, Madison, WI July 23 2006.
Saad M., A. K. Handa, Z. Deng, and P. S. Negi (2007) Ectopic expression of vis1, a small hsp, enhances plant growth and delays fruit ripening in tomato. Gordon Research Conference on Temperature Stress, California January 21-26 2007.
Invited Lectures at National, International conferences, Universities and Institutes
(A total of 46) Flagellar formation and shortening in Chlamydomonas reinhardtii. MSU/DOE Plant Research
Laboratory, Michigan State University, East Lansing, MI April 1978. Control of cell differentiation and development in lower plants. Research Division, Monsanto
Company. St. Louis, MO. September 1978. Cyclic AMP in lower plants. Department of Botany and Plant Pathology, Purdue University,
West Lafayette, IN. February 1979. Cyclic AMP-like compound(s) and its role in higher plants. Department of Horticulture, Purdue
University, West Lafayette, IN Oct. 1979. Resistance to water and salt stress of plant cells grown in vitro. Tissue Culture Association 31st
Annual Meeting - 1980. Use of plant tissue culture to improve crop productivity. Department of Horticulture, New
Mexico State University, Las Cruces, NM. August 1980. Use of plant tissue culture to improve crop productivity. Department of Horticulture, Purdue
University, West Lafayette, IN. October 1980. Regulation of soft rot disease in potato. Department of Horticulture, Purdue University, West
Lafayette, IN. March 1981. Resistance to water and salt stress through somatic cell selection. Shell Development Company,
Modesto, CA. May 1981. The use of plant cell cultures to study host- pathogen interactions. Session in Depth. Annual
meeting of Tissue Culture Association. Washington, D. C. June 1981. Tolerance to water and salt stress in cultured cells. A review. 5th International Congress of
Plant Tissue and Cell Culture, Tokyo, Japan. July 1982. Biological mechanisms underlying the regulation of soft rot disease. Molecular Biology Unit.
Tata Institute of Fundamental Research, Bombay, India. July 1982. Effect of ethephon on physiological and biochemical changes during the ripening of tomato fruit.
Indiana Canning Raw Products Conference. Purdue University. February 1983. Transposon mutagenesis and regulation of pathogenicity of Erwinia carotovora. Plant Cell and
Molecular Biology Seminar Series, Purdue University, W. Lafayette, April 1984. Pectolytic nonpathogenic mutants of Erwinia carotovora. The VI International Conference on
Plant Pathogenic Bacteria. College Park, MD. June 1985.
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Changes in gene expression during tomato fruit ripening. 13th International Congress of Biochemistry. Amsterdam, The Netherlands. August 1985.
Changes in gene expression during tomato ripening. Department of Horticulture, Purdue University. November l985.
Pathogenicity genes of Erwinia carotovora. Thid International Symposium of Molecular Genetics of Plant Microbe Interactions. Montreal, Canada, July 1986.
Gene expression in ripening tomato fruit. Purdue University. April 1989. Evidence for global regulation of the expression of pathogenicity genes in soft rot Erwinias. 7th
International Conference on Plant Pathogenic Bacteria Budapest, Hungary. June 1989. Molecular cloning and expression of pectin methyl esterase in tomato. Gordon Research
Conference on Postharvest Physiology. Andover, NH. July 1990. 3rd International Congress of Plant Molecular Biology, Tucson AZ, October 1991. (i) Antisense
RNA inhibition of pectinmethylesterase expression in transgenic tomatoes. (ii) Characterization of the promoter for the first enzyme of the shikimate pathway in potato.
Function of pectinmethylesterase in tomato fruit development and ripening. Gordon Research Conference on Plant Senescence. Plymouth State College - North. New Hampshire. July 1992.
Molecular engineering of fruit to improve its processing qualities including chemistry of pectins. Midwest Plant Biotechnology Symposium. Indianapolis. November 1992.
Field evaluation of genetically engineered tomatoes. Annual Meeting of Mid America Food Processors Association. Wapakoneta, Ohio. November 1992.
Molecular engineering of tomato fruit to enhance its processing qualities. Department of Horticulture, Ohio State University, Columbus. May 1993.
Pleotropic effects of pectin methylesterase antisense RNA gene in transgenic tomatoes. Symposium on Down Regulation of Gene Expression by Antisense and Other Technologies. Iowa State University, Ames. September 1993.
Modification of tomato fruit quality by suppressing gene expression of pectin methylesterase by its antisense RNA. Biotechnology Institute, Penn State University, College Park, PA. October 1993.
Role of pectin methylesterase in tomato fruit quality, shelf life and textural changes. USDA, ARS, Beltsville, MD, May 31, 1994.
Molecular engineering of fruit to impove its processing qualities including chemistry of pectins. The Consortium for Plant Biotechnology Research, 1994 Symposium. Chicago, November 1, 1994.
Enhancing processing efficiency of fruit by the molecular engineering of fruits to increase economic gains to processing industries. The Consortium for Plant Biotechnology Research, 1994 Symposium, Chicago, November 1, 1994.
Field evaluation of genetically engineered Ohio 8245 tomato lines. Annual Meeting of Mid America Food Processors Association. Fort Wayne, November 29, 1994.
Agronomical performance of genetically engineered Indiana tomato variety. Annual Meeting of Indiana Institute of Agriculture, Food and Nutrition. Indianapolis, March 30, 1994.
Enhancing Fruit Quality by Modifying Gene Expression, Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi September 27, 2000.
Molecular Engineering of Fruits to Enhance Fruit Quality Attributes, Indian Agricultural Research Institute, New Delhi. September 28, 2000
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Enhancing Fruit Quality by Modifying Gene Expression, The Centre for Biotechnology, Jawahar Lal Nehru University, New Delhi. September 29, 2000
Genetic Engineering of Fruit Cops, Department of Biotechnology Pune University, Pune October 3, 2000.
Molecular Engineering of Fruit. A symposium on “'Frontiers in Plant Signaling and Development in honor of Professor Johri, Tata institute of Fundamental Research, Bombay October 8, 2000
Molecular engineering of tomato fruit to enhance nutritional and quality attributes. The Fourth Annual Fall Symposium entitled Altering Plant Metabolism to improve human and animal health. Danforth Center, St. Louse, October 18-19, 2002.
Field performance of genetically engineered high lycopene tomatoes. Pharma farming a part of the "Biotechnology in the Service of Health" Symposium . Biotechnology Secretariat at The Annual Meeting of American Society of Chemists. New Orleans, Louisiana. March 23, 2003.
Enhancing Shelf Life and Nutritional Attributes of Fruits Using Molecular Engineering. Biotechnology Symposium- Agricultural Genetic Engineering Research Institute, Giza, Egypt January 11, 2004.
Molecular engineering of fruit to modify pectin chemistry (2004)Whistler Center for Carbohydrate Research, Purdue University, May 11, 2004.
Molecular engineering of fruit to modify pectin chemistry (2005) Biotechnology Symposium, Agricultural Genetic Engineering Research Institute, Giza, Egypt
Modification of fruit attributes to alleviate malnutrition: Enhancing lycopene levels by genetic engineering (2005). University of Agricultural Sciences, Bangalore, India, June, 2005.
Designing Crops for Human Health Benefits (2006) International Conference on Biotechnology Approaches to Alleviating Malnutrition and Human Health. University of Agricultural Sciences, Bangalore, India, January 9-11, 2006.
Modulating response to heat stress to enhance crop productivity and enhance biomass (2007). International conference on21 ST Century Challenges of Sustainable Agricultural Systems – Biotechnology, Environment, Nutrition, Trade and Policy 15th-17th March 2007
Collaborative Linkages (indicate collaborators, institution, and project):
Purdue : Dr. Bob Joly: Water relations in transgenic tomato plants Dr. Suresh Mittal; Production of vaccines in transgenic plants Bruce A Watkins: Lipid biochemistry Ray Bressan and Mike Hasegawa: Characterization of tagged transgenic tomato plants National: Dr. Autar Matoo, USDA/ARS, Beltsville, MD: Several projects on fruit ripening. Dr. Richard Ozminkowski, Heinz North America, Stockton, CA. Identification of genes
regulating processing quality of fruits. International: Dr. Yuval Eshdat, Agricultural Research Organization, Bet Dagan, Israel Oxidative stress. Dr. Avihai Perl, Agricultural Research Organization, Bet Dagan, Israel Oxidative stress.
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Dr. Doron Holland, Agricultural Research Organization, Bet Dagan, Israel Oxidative stress. Dr. David Levy, Agricultural Research Organization, Bet Dagan, Israel Oxidative stress. Dr. Saleh A Mohamed, National Research Center, El-Tahrir, Dokki, Cario, Egypt.
Postharvest of Apricot. Dr. Kailash Bansal, Indian Agriculture Research Institute, New Delhi. Gene regulation in
tomato using macroarrays.
Visiting Scientists and Postdoctoral fellows: Dr. N.K. Singh (1982-1984) Dr. Denys Charles (1985-1987) Dr. Satti Mohamed Satti (1985-1986) Dr. Henryk Rozycki (1986-1988) Dr. Varda Kagan-Zur (1991, 1992) Dr. Joel Gaffe (1992-) Dr. Krishna K. Mishra (1992-1998; 2004-2005 ) Dr. Beli R. Thakur(1992-96) Dr. Reid Fredrick (July 1993-Oct. 1994) Dr. Nanjundiah Prabha (March 1994-November 1994) Dr. Varda Kagan-Zur (June to August 1994) Dr. Galina Norova (March 1994-March 1995) Dr. Flyona Urmeeva (Oct. 1994-1997) Dr. Wusirika Ramakrishna (December 1997-November 1999) Dr. Jayanty G. Sastry (December 1997-1999 ) Dr. Chang-Kui Ding (December 1999-March 2000) Dr. Perla Venu (2003-2004) Dr. Alka Srinivastava (2004-2006) Dr. Pradeep Negi (2004-2005)
Major professor for: Completed:
Maurice Scott Biggs (Ph.D. 1982-1987) Regulation of polygalacturonase gene expression during tomato fruit ripening (Ph.D.) Assistant Professor, Ohio State University.
Sandra Jean Marlow (M.S. 1984-1987) Effects of heat stress on ripening associated polygalacturonase synthesis of tomato fruit (M.S.)
Robert Harriman (Ph.D. 1984-1990), Molecular cloning and regulation of expression of pectinmethylesterase in ripening tomato. Vice President, Research Scotts Company
Jiliang Chiu (Ph.D. 1984-1990), Molecular characterization of novel pathogenicity genes of soft-rot Erwinias. Assistant Professor, Tuskagee University, Tuskagee
John Henstrand (Ph.D. 1987-1991), Molecular characterization of wound induced promoters in tomato fruit. Scientist Biomarin Pharmaceutical Inc., Novato, CA.
Denise Tieman (Ph.D. 1989-1992), Genetic engineering of tomato to enhance fruit quality and shelf life. Resrch Associate, University of Florida
Mohan Ram (M.A. 1990-1992), Characterization of heat stress responses in tomato fruit. Research Leader, Monsanto, Bangalore, India
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Kurt Kausch (Ph.D. 1990-1994) Molecular cloning of promoters expressed during ripening of tomato fruits.
Larry Allen Turner (Ph.D. 1993-1996) Characterization of pectinmethylesterase promoter. Martin Tiznado Hernandez (Ph.D. 1993-1999) Molecular role of pectinmethylesterase in
plant growth and development . Zhiping Deng (Ph. D. 1997-2000) Molecular cloning of fruit quality genes. Jerson D. Dominguez Torres (Ph. D. 1997-2003) Characterization of fruit specific
promoters. Aparna Bhaskaran (Master of Ag. 1999-2004) Functional genomics of fruit quality
attributes. Zidian Xie (PhD 2002- Left without completing a degree) Mohammad Saad (Ph.D. 2003-present ) Modulating response to heat stress by engineering
small heat shock proteins to enhance crop productivity Savithri Nambeesan (Ph.D. 2004- ) Dissecting roles of polyamines in plant growth and
development using forward and reverse genetics
Committee member for:
Ronald W. Skadsen (Ph.D., 1984) Cristina Lopez-Peralta (Ph.D., 1985) R. K. Jawaswal (Ph.D., 1985) Craig Pikaard (Ph.D., 1986) Marla Binzel (Ph.D., 1987) Susan Schoneweis (M.S., 1988) Kay Ann Lawton (Ph.D.) Hong Wang (Ph.D.) Jan Prke (M.S.) Zutang Chen (Ph.D.) Jim Jones (Ph.D.) John Ray (M.S.) Xiaomu Niu (Ph.D.) Chunming Liu (Ph.D.) Sven Verlinden (Ph.D.) Shuli Yang (Ph.D.) Sandra Lay (M.S.) Lise Lotte Dock (Ph.D.) Jennifer Boatright (Ph.D.) Deepa Varadarajan (Ph.D.) Zarir Vaghchhipawala (Ph.D.) James Baldwin (Ph.D.) Lisa Bushue (M.S.) Fahed Synan Abu Qamar (Ph.D.) Jaffal Khaldoun Al-Hadid (Ph D.)
Undergraduate Research Project
Ivan Delgado
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Research grants and awards received:
1980 Resistance to water and salt stress through somatic cell selection. BARD (with R. A. Bressan and P. M. Hasegawa) October 1980 - October 1983. $105,000.
1981 In vitro selection of plants resistant to early blight and high temperature stress. Title XII. (with R.A. Bressan and P.M. Hasegawa) October 1980 - September 1981. $10,000.
1982 Evaluation and exploitation of genetic variability from adventitious potato plants derived from tissue culture. Native Plants, Inc. October 1, 1982 - September 30, 1986. $120,000.
1982 Plant cell and tissue culture research. 1982-1984. AES Program Improvement Funds (with R. A. Bressan and P. M. Hasegawa). $90,000
1982 Salt stress tolerance in plants. Native Plants, Inc. September 1982 to March 1983. (with P. M. Hasegawa and R. A. Bressan). $10,000
1983 Biological mechanisms underlying the regulation of synthesis of pectolytic enzymes in food microorganisms. Food Utilization Funds. July 1, 1982 - June 30, 1984. $13500
1983 Development of tissue culture systems to produce important plant secondary natural products. Showwalter Trust 1983-1984, (with P. Heinstein, P. M. Hasegawa and R. A. Bressan). $30,000
1983 Regulation of protein and mRNA metabolism in salt tolerant and intolerant cultured higher plant cells. DOE (with R. A. Bressan, P. M. Hasegawa, and Native Plants, Inc.). October 1983 to September 1984. $80,000
1984 Biochemical studies on the role of polygalacturonase and ethylene in tomato fruit ripening. AES Assistantship July 1, 1982 - June 30, 1984. $14,000.
1984 Biological mechanisms underlying the regulation of soft rot disease. David Ross Grant. September 1, 1982 - August 30, 1984. $13,200.
1984 Measure of the modulation of solute and metabolic pools in the cytosol and vacuole of plant cells. Binational Science Foundation (with R. A. Bressan, P. M. Hasegawa, N. C. Carpita, D. Delmer, H. R. Lerner and A. Poljakoff-Mayber). $70,000 1984-1986
1984 Molecular control of polygalacturonase gene expression during the ripening of tomato fruit. AES. July 1984 to June 1986. $14,000
1984 Molecular mechanisms regulating the pathogenicity of Erwinia carotovora. David Ross Grant. July 1984 to June 1986. $13,200
1984 Regulation of protein and mRNA metabolism in salt tolerant and intolerant cultured higher plant cells. DOE (with R. A. Bressan, P. M. Hasegawa, and Native Plants, Inc.). October 1984 to September 1985. $80,000
1985 Isolation and modification of genes involved in salt stress tolerance in plants (with R.A. Bressan and D.N. Kuhn). USDA Competitive Research Grant Program. $150,000. September 1985-December 1988.
1985 Molecular basis of pathogenicity of Erwinia carotovora. CST January 1985 to December 1985. $50,000.
1985 Molecular cloning of pathogenicity genes of soft-rot Erwinias. USDA. July 1985 to June 1987. $85,000.
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1985 Regulation of protein and mRNA metabolism in salt tolerant and intolerant cultured higher plant cells. DOE (with R.A. Breman and P.M. Hasegawa). October 1985 to September 1986. $80,000.
1985 Role of cell membrane in water and salt stress tolerance. AES Program Improvement Funds (with P. M. Hasegawa and R. A. Bressan) January 1985 to December 1986. $30,000
1985 Role of cell membrane in water and salt stress tolerance. AES Program Improvement Funds (with P.M. Hasegawa and R.A. Bressan) January 1985 to December 1986. $30,000.
1986 Effects of tomato polygalacturonase antisense RNA production on the ripening of tomato fruit. AES $14,000.1986
1986 Molecular basis of pathogenicity of Erwinia carotovora. CST January 1986 to January 1987. $75,967.
1986 Molecular cloning and characterization of expression of genes responsible for enhanced pathogenicity of Erwinia carotovora at low oxygen tensions. David Ross Grant. July 1986 to June 1988. $13,200.
1989 Regulation of tomato ripening and fruit storage life by the use of heat and salt stresses. (with J. H. Cherry, Y. Mizrahi and V. Kagam-Zutz) BARD. $200,000. September 1989-August 1992.
1991 Postharvest shelf life of shredded lettuce (with J. Floros and P. Nelson) Value Added Center $28,000. April 1991-March 1992.
1991 Developing controls for soft-rot disease of stored potatoes. International Programs. $5,000. August 1991-July 1992.
1991 Genetic engineering of tomato. H.J. Heinz Company. $40,000. January 1991-April 1992.
1991 Regulation of soft-rot Erwiniae virulence genes. USDA/NRICGP. $60,000. September 1991-August 1993.
1991 Transgenic tomato plants. Mid America Processor Association $2500. April 1991 to March 1992
1992 Genetic engineering of tomato. H.J. Heinz USA, $40,000. May 1992-April 1993. 1992 Molecular engineering of fruit to improve its processing qualities including chemistry
of pectins. Midwest Plant Biotechnology Consortium. $194,000. July 1992-June 1994. 1992 Transgenic tomato plants. Mid America Processor Association $2000. April 1992-
March 1993. 1993 Creation and characterization of transgenic tomato fruits simultaneously expressing
antisense RNA genes for polygalacturonase and pectin methylesterase. PRF Research Grant. $20,000., 1993-1995
1993 Field evaluation of transgenic tomatoes with increased soluble solids. New Crops Centers. $5,000. April 1993-March 1994.
1993 Genetic engineering of tomato. H.J. Heinz Company. $40,000. May 1993-April 1994
1993 Transgenic tomato plants. Mid America Food Processor Association. $3,500. April 1993-March 1994.
1994 Evaluation of genetically engineered Ohio 8245 tomato lines with improved processing attributes for release. Mid America Food Processors Association. $2000. April 1994-March 1995.
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1994 Field Evaluation of genetically engineered Indiana tomato varieties. Value Added Grant from Indiana State. $26,000. July 1994-June 1995.
1994 Genetic Engineering of Tomato. H.J. Heinz USA. $40,000. May 1994-April 1995. 1994 Improvement of fruit quality by genetic engineering. Consortium for Plant
Biotechnology Research, Inc. A USDA Funded Program. $85,000. Dec 1994-Dec 1996.
1994 Improvement of Indiana tomato varieties by genetic engineering. Value-Added Grants. $25,000. July 1993-June 1994.
1994 Molecular engineering of fruits. Procter and Gample Company. $2000. 1995 Field trials of transgenic tomato variety Ohio 8245. Mid America Food Processor
Association. $1,500. May 1995-April 1996. 1995 Function of pectin methelesterase in plant growth and development. USDA/NRICGP
- under Plant Growth and Development Program Area. $130,000. 1995 Genetic engineering of tomato fruit. H.J. Heinz. $40,000. May 1995-April 1996. 1995 Molecular characterization of a fruit ripening specific lipoxygenase promoter.
Pioneer Hi-Bred. $10,000. Nov, 1995-Oct 1997. 1995 Selection of genetically engineered tomato lines with improved processing attributes
for release to Indiana tomato growers. Indiana Value Added Grant Program. $25,000. July 1995-June 1996.
1996 Role of pectin methylesterase and pectin chemistry in plant growth and development (with Bob Joly). USDA/NRICGP - Plant Growth and Development Section. $87,366. October, 1996-September, 1999.
1996 Role of ubiquitously expressed pectin methylesterase in plant growth and development. Purdue Research Foundation. $21,000. August 1996-July 1998.
1996 Tomato research. H.J. Heinz. $40,000. May 1996-April 1997. 1997 Tomato research. H.J. Heinz. May 1997-April 1998. $9,600 1999 Functional genomics of fruit quality attributes: Isolation and functional
characterization of fruit quality genes. PRF Purdue University. Award ~$ 28,000. September1, 1999-August 31, 2001.
1999 Identification of genes regulating fruit processing quality. Heinz $20,000. September1, 1999-August 31, 2001.
1999 Molecular Markers for Breeding Tomato Fruits with Enhanced Processing Attributes. Mid America Food Processors Association. April 1999-April 2000. $1500. September1, 1999-August 31, 2000.
2000 Enhancing Quality Attributes Of Potato And Tomato By Modifying And Controlling Their Oxidative Stress Outcome. BARD. PI: A. K. Handa; Co-PI: Yuval Eshdat Agricultural Research Organization, Bet Dagan, Israel. Award $300,000 (Handa Program $164,000) September1, 2000-August 31, 2003.
2000 Novel methods to sanitize fruits and vegetables using chlorine dioxide gas. Richard Linton, Philip Nelson and Avtar K. Handa . USDA/NRI. Award $475,000 (Handa Program $55,000). September1, 2000-August 31, 2003.
2001 Functional genomics of fruit quality attributes. The 1999 ARP Genomic Grant program. $5,000. September1, 2001.
2001 Improving the efficiency of processing tomato production: Managing color disorders. USDA/IFAFS (in collaboration with Ohio State University) Total Funding $600,000; Handa Program $115,674. September1, 2001-August 31, 2004
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2003 Functional genomics of fruit quality attributes. $ 10,000: Heinz 2003. 2003 Modulating phytonutrient content in tomatoes combining engineered polyamine
metabolism with photomorphogenic mutants. BARD, the United states-Israel Binational Agricultural research Development Fund, Bet Dagan, Israel. Levin I., Handa AK, Mattoo AK, Lalazar A. Award $ 330,000 (Handa Program $99,000). October 1, 2003-September 31, 2006.
2004 Identify gene/promoter that effect lycopene levels in ripened tomato fruits. $ 10,000: Heinz 2004
2004 Partnering with higher education in India for improving nutritional quality of food by biotechnology approaches (with Drs. Raghothama, Salt, Goldsbrough, Martin, Orvis, Sammons) ALO-USAID July 2004- June 2007, $300,000 Handa share $75,000
2006 Identification and classification of the genotype-specific modifications of tomato cell wall architecture that gives rise to desired quality traits. Heinz, $68,625
2006 Role Polygalacturonase and Pectin Methylesterase Activities play in determining processing quality of Tomato Products, California League of Food Processors, Nov 2006-May 2007), $ 19000
2006 Use of gene transfer techniques for production of new tomato and strawberry variety tolerant to nematodes. U.S.- Egypt Sci. & Tech. Joint fund. (with Dr. Mohei Solliman, National Research Center, Dokki, Egypt) August 2006- July 2009. $60,000 (Handa share, $ 30,000)
Patents: United States Patent Number 6355862: Avtar K Handa and Kurt D. Kausch, Improvement of fruit quality by inhibiting production of lipoxygenase in fruits. Issued on March 12, 2002.
