0
10
20
30
40
50
60
70
80
90
FPKM
of C
D28
rela
tive
to T
-cel
l sig
natu
re
ICO
S-CD
28 fu
sion
D
124V
AITL Normal
D12
4E +
T19
5P
T195
P
TFH Teff Naïve CD4+
p<0.0001
T195
P
A
B C D
A.
Case 089 ACT>CCT p.T195P
Case 086 GAC>GAG p.D124E
B. C.
Case 086 ACT>CCT p.T195P
CD80 IgV-containing Ligand bound A B C
D124
L141
V124
D E F
G H I
-5.00
5.00
CTLA4
CD80 K130
L141 CD28 WT
CD80 K113
D124
K127
CD28 D124V
CD80 K113
V124 K127
J K L
M N O
L141 D124 V124
CTLA4 CD28 W
T CD28 D124V
R94 R94 E81 E81 R94 E81
K20 K20
Supplemental Figure 3
CD28 WT
CD28 mutant
p<0.001p<0.007
p<0.009p<0.04
p<0.03p<0.004
p<0.002p<0.04
A B C
D E
F G
H I
Supplemental Figure 4