Biological Research in CanistersBRIC-LED

• Up to six 60mm x 15mm Petri Dishes per BRIC canister

• Eight canisters per half locker tray

• 3 sealed levels of containment• Unidirectional LED illumination• Single or dual stage manual

liquid (fixatives, growth inhibitors, etc.) introduction

• Autonomous environmental data logging

Petri Dish

BRIC-LEDCanister

Actuation tool

interfaceIllumination

controls

Sealed Petri Dish Fixation Unit

Liquid reservoir

1Enables Liquid Treatments (e.g. Cytochalasin) Followed by In-flight FixationESC 00415—EAR 99

Biological Research in CanistersBRIC-Opti

• 2 sealed levels of containment

• Gas sampling capable• Passive• Can be frozen (-100 °C)• Contains up to 4 OptiCell™

culture chambers• Autonomous environmental

data logging

OptiCell™Culture

Chambers

Multichannel temperatureand humidity logger

Base

Lid

Gas sampling port

OptiCell™CultureChambers

2For Passive Tissue Culture/Microbial Growth in COTS OpticellsESC 00415—EAR 99

Kennedy Space Center Fixation Tube(KFT)

Actuator

Main Tube

Sample Tube2.365” L x 0.875” D

Expansion Plug

Chemical Preservative:• 3% glutaraldehyde• 5% formaldehyde• 5% DMSO• RNAlater™• b-glucuronidase stain• 5% formalin 5% acetic acid 63% ethanol• 0.5% glutaraldehyde 2% paraformaldehyde

• Provides three levels of containment during all phases

• No glovebox required• Samples may be transferred from other growth

facilities • Maintains robust containment down to -100 °C

3For Crew-Facilitated In-Flight Specimen FixationESC 00415—EAR 99

FASTRACK™ Suborbital Platform

• Provides compatibility across the entire fleet of emerging suborbital vehicles, existing and future parabolic flight providers, and future orbital free-flyers or transports

• Supports 2 single middeck locker equivalents or 1 double locker

• Provides power and data acquisition

FASTRACKStructural

View

4Provides a Standard Platform for Suborbital and Parabolic Investigations

Parabolic Flight Testing

ESC 00415—EAR 99

Payload Integration & Operations

• 52 payloads have been managed and integrated• Platforms: shuttle, ISS, SPACEHAB, Progress, MPLM,

Spacelab, Mir• Joint foreign collaborations with CSA, DLR, ESA, JAXA,

NSAU• Capabilities for hardware engineering, integration,

operations support, and project science coordination• Experiment Monitoring Area (EMA) operations control

center at KSC for real time support• ISS Environmental Simulator (ISSES) chambers provide

simulated spaceflight environment for analog ground controls

5Integration History and CapabilitiesESC 00415—EAR 99

Payload Development:A Phased Approach

6Payload LifecycleESC 00415—EAR 99

Pre-Phase A

Solicitation

& Selection

Phase A

Definition

Phase B

Development

Phase C

Verification

Phase D

Integration

Phase E

Operations

& Analysis

• Each phase includes a final milestone or gate• Each phase has distinct activities associated with science, hardware, and

analytical integration• Phase duration is a function of many factors

– Experiment maturity– Hardware maturity– Platform

Payloads in the land of “OZ”

7Why an Implementation Partner is ImportantESC 00415—EAR 99

PDR CDR CoFRPayloadPre-Ship

PayloadManifested

TACTICALSTRATEGIC

Key:P – PreliminaryU – UpdateF – Final

SafetyPhase II

SafetyPhase 0/I

SafetyPhase III

Payload Integration Manager Schedule

• Export Classification Letter• Prelim Design Data Package• Safety Data (Phase 0-I)• Training Strategy TIMs

• Resource Requirements Definition• Procedures & Displays DS (P)

• Critical Design Data Package• Safety Data (Phase II)• ICD/Verification Requirements

• Payload Integration Agreement (PIA)

• C&DH DS (P)• KSC Support Rqmts DS• KSC Technical Rqmts DS• Ground Data Services DS• Procedures & Displays (U)• Payload Planning DS (P) • Manifest/Stowage DS (P)• Drawing DS (P)

• Safety Data Pack

• C&DH DS (U)• Payload Trainer H/W & S/W• Training Plans & Courseware• Payload Planning DS (F)

• Interface Control Document (ICD) (U)• Payload Verification Plan (PVP) (U)• Requirements Exceptions

• CoFR Endorsement

• Manifest/Stowage DS (U)• Drawing DS (U)• Integration Data Pack (IDP)

• C&DH DS (F)• Procedures & Displays (F)

Acronyms

ABRS Advanced Biological Research SystemBRIC Biological Research in CanistersBRIC-LED Biological Research in Canisters – Light Emitting DiodeCOTS Commercial Off-the-ShelfCSA Canadian Space AgencyDLR Deutsches Zentrum für Luft- und RaumfahrtDMSO Dimethyl sulfoxideECP Environmental Control PanelEMA Experiment Monitoring AreaERC Environmental Research ChamberESA European Space AgencyEXPRESS Expediting the Processing of Experiments to the Space StationGCU Generic Containment UnitGFP Green Fluorescent ProteinISS International Space StationISSES ISS Environmental SimulatorJAXA Japan Aerospace Exploration AgencyKFT Kennedy Space Center Fixation TubeKSC Kennedy Space CenterLSSC Life Science Services ContractMPLM Mult-purpose Logistics ModuleNSAU National Space Agency of UkraineTOSC Test and Operations Support Contract

8ESC 00415—EAR 99

Points of Contact

9

Hardware + Implementation Point of Contact (ESC):David W. ReedPayload Engineering LeadMail Code ESC-24Kennedy Space Center, FL 32899Phone 321.861.2980David.W.Reed@nasa.gov

Ground Research Support Point of Contact (ESC):Michele N. BirmeleAdvanced Life Support Laboratories LeadMail Code ESC-24Kennedy Space Center, FL 32899Phone 321.861.2940Michele.N.Birmele@nasa.gov

Laboratory Support Point of Contact (TOSC):Bruce E. BurchTOSC Project ManagerMail Code 721S-S100Kennedy Space Center, FL 32899Phone 321.867.5255Bruce.E.Burch-1@nasa.gov

ESC 00415—EAR 99

Thank you!

10ESC 00415—EAR 99