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BIOFILMS

Baby mize Shinu

1613852 Asst professor

M.sc. MMIMSR

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AEROBICCONDITION

ACID PRODUCE ATTHE SURFACE

OXIDATIVE

ANEAROBICCONDITION

ACID PRODUCETHROUGH OUT THE

TUBE

FERMENTATIVE

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IS!"R#

1684 - Van leewen!e" #$enta% p%a&ue

1$4% # He&"ele"'an ( Helle) #

*Bott%e'()ect*

1$4+ # ,!ell - a+hesions

1$6$ #.!ne/ # +i)erentor,anisms

Matri-compose+ of

po%ysacchari+e

1$0+ # a)a"l'/ #antimicrobia%

resistant

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BI"I0MS

Biofilms may form on living or

non-living surfaces MO have 2 forms SESSILE &

PLAN!ONI"

BIO#ILM can $e sim%le or

com%le Sim%le MONOSPE"IES

"om%le M'L!ISPE"IES O(

"O- BIO#ILMS )M"*

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$(I/I!I"/

BIO#ILM - assem$lage of $ac+erial cell -irreversi$ly associa+e,

i+h +he surface an, enclose, in ma+ri of E+racellular Polymeric

Su$s+ance )EPS).

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SURFACES

(ough surface

,ecrease shear force

increase surface area .y,ro%ho$ic surface

Non %olar

more on !eflon +han

glass or me+als

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G!!2

'!3l

NUTRIENTS

5H O 7 CO

TEMPERATURE

EN/I(ONMEN!AL #A"!O(S

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S!(S I/ BI"I0M$((0"(M(/!

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S!A( 1' R((RSIB0(BI/$I/

!a0es secon,s +o ini+ia+e

"on,i+ioning film ) Loef & Neihof

134*

Nu+rien+ rich environmen+

a,herence

/an,er all forces

(eversi$le some cells ,e+ach from

su$s+ra+um

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BI/$I/

Minu+es af+er s+age 1

A,hesion molecules 5 %illi5 fim$riae

"ell aggrega+es

EPS is %ro,uce,

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(4!RA(000AR "0#M(RISBS!A/( 7(S

Gl!al9 7 /l'elae)

H':l 2)a;e2

DNA 7 PROTEINS (POL

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BI"I0M 9 :"RM S(/SI/

CELL ;! CELL!&n'a;'!n

Re/5!n/'le =!) ;e

e95)e//'!n != >')&lene=a;!)/

GRAM POSITIVE #!l':!5e5;'2e/ !) 5)!;e'n

GRAM NEGATIVE N-al !!/e)'enela;!ne

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S!A( 3

Ma;&)a;'!n I /;a:e

T'"ne// 'n)ea/e ;!!)e ;en 1%?

Ba/e e!e/anae)!'

S!A( ;

Ma;&)a;'!n II/;a:e

T'"ne//'n)ea/e ;! !)e

;en 1%%?

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S!!R( " BI"I0M

"!(RM"S! 0A#(R

E%ose, +o high concen+ra+ion

of nu+rien+s an, oygen

Mos+ ac+ive organisms %resen+

Aligne, closely

May slough off +o coloni6e

ne $iofilm

SECOUND LAe) a;'>e

E9an:e :enea;e)'al M&l;'5le 2)&:)e/'/;ane

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I//(RM"S!

Earlies+ %ar+ of $iofilm leas+ ac+ive

A++ache, +o su$s+ra+um

Per sis+er cells

slo groing

analogous +o $ac+erial s%ores

main+ain gene %ool

.ighly resis+an+ +o environmen+s+ress & an+imicro$ial agen+s

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S!A( 5

Cell/ 2'/5lae ( /5)ea2;! !l!n'@e new/&)=ae/

En@e/ ;a; el5 'n

2'/5e)/'!n 2e:)a2'n:EPS a)e

DISPERSIN B

DEOX

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A!"(/II!#

C!l!n'@a;'!n

Pa:!;!/'/

Gene ;)an/=e)

An;''!;' )e/'/;ane

Me;a/;a/'/

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"0"/I

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BI"I0MS 9 R"!(!S

R"M A"#!"SIS Pa:!;e/ a)e &nale ;!

ee;'>el en:&l= a a;e)'&2&e ;! /;'" EPS

T'/ a&/e 5a:!;e/ ;! )elea/ela):e a!&n;/ != 5)!-'naa;!) en@e/ an2;!"'ne/ 7 lea2'n: ;!

'naa;'!n an2 2e/;)&;'!n !=nea) ;'//&e/

BI"I0MS

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BI"I0MS(0 (/(

!RA/S(R Gene ;)an/=e) an

!n>e); a 5)e>'!&/>')&len; !en/al/!):an'/ 'n;! a ':l>')&len; 5a;!:en

Cl!/e 5)!9'';

T)an/=!)a;'!n (!n&:a;'!n

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A/!IBI"!I!(RA#

Ba;e)'a :)!w'n: 'n

a '!3l a)e ':l

)e/'/;an; ;!an;''!;'/ 7 &5 ;!17%%% ;'e/ !)e)e/'/;an;

EPS GENE TRANSFER

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BI"I0MS I/ #S!IIBR"SIS

In cystic >brosispatients haeseu+omonasinfections thatoften resu%ts inantibiotic resistant

bio>%ms.

A!(!(RS A/$

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A!(!(RS A/$BI"I0MS

Inne) an2 !&;e) /&)=ae/

Te !):an'/ !!nl!n;a'na;'n: a)e

S. epidermidisE. faecalis

E. coli

P. mirabilis

p. aeruginosaK. pneumoniae

L!n:e) 2&)a;'!n !=a;e;e) lea2/ ;! UTI

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"/!A!0(/(S

C!n;a; len/e/ C!n;a; len/

/;!)a:e a/e/ R'/" =a;!) 'n

!n;a; len/a//!'a;e2 !)neal'n=e;'!n/

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I/!RA!(RI/( $(I(S

Can2'2a al'an/

En;e)!!&/

S;a5l!!&/ a&)e&/

C!a:&la/e ne:a;'>e/;a5l!!&/

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Arti>cia% hip prosthesis

C!a:&la/e ne:a;'>e/;a5l!!&/

En;e)!!&/

P/e&2!!na/ ae)&:'n!/a

S;a5l!!&/ a&)e&/

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$ 7 centers for +iseasecontro%

6 != nosocomia%infections a)e a&/e2 '!3l/

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LAB DIAGNOSIS OF BIOFILM

DIAGNOSTIC

IMAGING CONVENTIONAL

MICROBIOLOGY

BROTH CULTURE MAKIS METHODE QUANTITATIVE

CULTURE

VORTEXING ULTRASONIFICATIO

N

S!AI/I/ MIR"S"# !B( A$(R(/(

M(!"$

M"$II($ "/" R($AAR M(!"$

!ISS( 0!R( 0A!(M(!"$

RA$I" 0AB(00I/ I/SI! #BRI$ISA!I"/

!B( A$(R(/( M(!"$

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!B( A$(R(/( M(!"$

Inocula+e on B.I $ro+h )27 sucrose *

incu$a+e ) 28 hours a+ 93:"*

;ash se,imen+ i+h PBS )%. 3

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M"$II($ "/" R($ AARM(!"$

Inocula+e on Mo,ifie, congo re,

agar an, incu$a+e for 28 +o 8>

hours a+ 93:c

Blac0 coloure, colonies i+h ,ry

crys+alline consis+ency in+er%re+e,

as %osi+ive $iofilm %ro,ucing s+rain

"

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M(!"$

Inocula+e, on B.I $ro+h )27 sucrose* incu$a+e, 1>hours a+

93 :" 5 ,ilu+e, 1 in 1== i+h fresh me,ium

ells fille, 2==?l ali@uo+s of ,ilu+e, cul+ures

!"P incu$a+e, for 28 hours a+ 93:"

ashe, i+h =

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Mean "$ a%ues Bio>%mformation

%1% N!n wea"

%1% # %4% M!2e)a;e

%4% H':

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TREATMENT

An+i$io+ics (emoval of

in,elling me,ical

,evices

'sing $ac+erio%hage

Inhi$i+ion of @uorum

sensing

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P(OP.LACIS Allo%las+ic ma+erials has +o $e re%lace

(emoval of in,elling me,ical ,evice

'sing $ac+erio%hage

Inhi$i+ion of @uorum sensing

"areful choice of an+i$io+ics an, early

+hera%y may lea, +o era,ica+ion of

con,i+ion

Ase%+ic inser+ion of ca+he+er

Avoi, inser+ion of ca+he+er if %ossi$le

Shor+en +he ,ura+ion of ca+he+eri6a+ion

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THANJ