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Bacterial Transformation Teacher Preparation Materials Lists
Bacterial TransformationTeacher Preparation
Materials Lists
Materials List-Item 1(per group of students)
• 1 aluminum foil packet containing 4 sterile paper clips that are large and smooth. The clips should be opened into a 90° angle and the small end bent to close it.
Bacterial TransformationTeacher Preparation
Materials List -Item 2(per group of students)
• 1 aluminum foil packet containing 4 sterile paper clips that are small and smooth. Bend one end of each paper clip into a loop.
Bacterial TransformationTeacher Preparation
Materials List -Item 3(per group of students)
• 2 microcentrifuge tubes (1.5 ml) containing 2 drops of sterile CaCl2 and labeled "CaCl2"
• Place on ice in a refrigerator until ready to use.
Bacterial TransformationTeacher Preparation
Materials List -Item 4(per group of students)
• 1 petri dish containing only nutrient agar and 1 dish with ampicillin labeled "No Amp" on the bottom
Bacterial TransformationTeacher Preparation
Materials List -Item 5(per group of students)
• 1 petri dish containing nutrient agar and 1 dish with antibiotic ampicillin labeled "Amp" on the bottom
Bacterial TransformationTeacher Preparation
Materials List -Item 6(per group of students)
• Copies of the laboratory instructions, one for each student
Bacterial TransformationTeacher Preparation
Materials List -Item 7(per group of students)
• 1 glass bottle with a cap containing sterile nutrient broth and labeled "LB Broth"
Bacterial TransformationTeacher Preparation
Materials List -Items 8&9 (per group of students)
• 1 Sharpie marking pen
• 1 rubber bulb for the pipettes
Bacterial TransformationTeacher Preparation
Materials List -Item 10(per group of students)
• 1 aluminum foil packet containing the glass parts of 4 sterile pasteur pipettes or sterile plastic pipettes
Bacterial TransformationTeacher Preparation
Materials List -Item 11(per group of students)
• 1 aluminum foil packet containing 8 sterile toothpicks
Bacterial TransformationTeacher Preparation
