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BasicBiological
Safetyor
Why I Cant Drink My Coffee While
Playing With Bacteria
Frank A. Cantone, Ph.D., CBSP
Biological Safety Officer
Environmental Health & Safety
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BiohazardAn agent of biological origin that has the capacityto affect the health of humans, animals, and or
plants . Included are:
Microorganisms such as bacteria, fungi, viruses, parasites
Toxins
Human blood and products
Infected animals and tissues
Recombinant products
Allergens
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Protect students/employees from acquiring work-
related infectious diseases
Prevent contamination of the environment
Provide guidance and principles for the safe use of
biohazardous materials and infectious agents
Goals of Biological Safety
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Reservoir and
concentration of
infectious agentPortal of escape
Transmission
Route of entry/
infectious doseSusceptible host
Incubation
Practices/Equipment
PPE
Immunization/Preventive Therapy
Chain of Infection
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Risk Assessment
Conservative approach, Universal Precautions
Identity and pathogenicity of the agent
disease incidence and severity
agent stability
origin
Route of transmission- aerosol,
percutaneous, ingestion, contact
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Risk Assessment Concentration and infectious dose
host-pathogen interaction
activity/volume
Availability of treatment and/orpreventative measures
vaccines, antibiotics
medical surveillance
Experience, skill, and condition of lab
personnel
immune status
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ENGRI 1310 Risk Assessment
C6 rat astroglial cell line. Non infectious. Lowrisk.
Cell lines pose low risk. Any potential viral orother pathogenic risk factors would be subcultured
out. Primary cells (cells extracted directly from an
organ) carry the highest risk since they could haveexisting viruses and pathogens
Cell lines from non-primate sources have lowerrisk factors.
Handle as a Biosafety Level 2 material.
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BME 3020 Risk Assessment
C6 rat astroglial cell line. Non infectious.Low risk. Handle under Biosafety Level 2
requirements.
Human HeLa cells. Risk increases withhuman cell lines, however, still low non-
infectious. Handle under Biosafety Level 2
requirements
Human Neutrophils. Will be handled by
instructional staff that has been trained in
handling blood borne pathogens. Biosafety
Level 2
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BME 4020 Risk Assessment
Crawfish Low risk level. Biosafety level 1
Handle as you would handle any meat
product in your home. However, we will
handle as a Biosafety level 2 material.
Pancreatic cells (mammalian-hamster). Low
risk level. Handle under Biosafety level 2
requirements
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Biosafety Level 1 (BSL1)
Agents not ordinarily associated
with disease
Basic level of containmentStandard microbiological
practices
No special primary or secondarybarriers
Hazard Management
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Hazard Management
BSL2Indigenous, moderate-risk agents
Percutaneous, ingestion, or mucous
membrane exposuresBSL1 practice plus:
Signage
Sharps precautions
Biological safety cabinet if
splash or aerosol hazard
Personal protective equipment as needed
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Laboratory Practices & Techniques
Standard laboratory practices DO NOT eat, drink, smoke, apply
cosmetics or handle contact lenses inwork areas
Avoid contact with mucous membranes
Use mechanical devices when pipetting
Use designated refrigerators
to store food or beverages
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Laboratory Practices & Techniques
Handwashing
Every time you remove
gloves, handle infectious
material Ordinary soap OK
Waterless antiseptic hand
cleanser OK
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Laboratory Practices & Techniques
Decontaminate work surfaces Selection
Degree of microbial killing required
Nature of item/ surface to be treated
Safety, ease of use, and cost
Efficacy
Type and number of organism
Amount of organic material present
Type and concentration of germicide
Time, environmental conditions
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Personal Protective Equipment
Use of PPE Appropriate for the task
gloves, masks, eyewear, and impervious gowns for
splash hazards
Use each time you perform task
If damaged or contaminated, remove as soon as
possible
Remove all PPE before
leaving workplace
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Disposal of RegulatedMedical Waste
Segregation of RMW into:
red biohazard bags
sharps containers
treated liquid waste- sanitary sewer
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BME 1310, 3020, 4020 Labs
Crawfish, C6, HIT pancreatic cells, HeLa
Gloves to be worn at all times when
handling tissue or cells.
All solid material in contact with cells to be
disposed of in red biohazard bags.
Liquid waste should be placed in bleach
containers
Clean all surfaces with 70% ethanol
