Basket CentrifugeApplications in Insulin Production

Catherine ChaoDanielle TurneyDana Zoratto

Background

Purpose: to remove the WFI and insulin from the insulin crystal

Basket Centrifuge Criteria

Separation Liquid-SolidSolids Content 5 - 60%Particle Size 5 - 10,000 µmG-Force 300 – 1500Rotational Speed 2000 rpm maxDiameter 1500 mm maxDepth 1000 mm

Basket Centrifuge Operation

Feed Slurry

Spin Dry Wash

Discharge

Spin Dry

General Operation:

Process Options:Operation:• Batch• Semi-Continuous

Modes of Discharge:• Centrifuge stopped• Centrifuge decelerated to slow

speed• Discharge at full speed

Basket Centrifuge Types

Perforated Basket Centrifuge• Filtration• High solid concentration • Dewatering necessary

Imperforate Basket Centrifuge• Sedimentation• High solid concentration• Dewatering not necessary

Centrifuge Type Selection

• Vertical Perforate Basket Centrifuge

Solid Inlet Content 35.2% (by mass)

Inlet Flow 1.44 L/hInsulin Crystal Size

125x125x80 µm (minimum)180x180x160 µm (maximum)

Process Specifications:

Type Selection:

Justification of Selection

Schematic Diagram of a Basket Centrifuge

Performance Specifications of Various Centrifuges

Vertical Basket Centrifuge

Filter Area 2 – 5 m2

Filter Specification 37 - 3000 µm Diameter-to-height Ratio 1:1 – 3:1Acceleration 900 – 1800 rpmCost (USD) 7702.8 +

143,279*[Diameter (m)]

Centrifuge Specifications:

• carbon or stainless steel• Monel• Inconel• Titanium• rubber and plastic coatings (various)

Materials of Construction:

Vertical Basket Centrifuge

Slow-load Slurry Through Pipe Acceleratio

nDeceleration

Discharge Through Bottom

Cake Removal

Operation Flow Diagram:

• Batch Operation• Cake removal: single or multiple ploughs between cycles• High solids recovery in retentate• High wash efficiency• Loading and discharge at reduced speeds

System Specifications:

Model Selection

Basket Centrifuge

Perforated

Imperforate

Vertical(discharge at low speed)

Horizontal(discharge at high speed)

Batch(discharge while stopped)

Krauss-Maffei PT Vertical Centrifuge VZU

Broadbent IPD Deep Basket Centrifuge

Krauss-Maffei PT

Size: 1000 x 630 mmCapacity: 250 LFeed Requirement: >3% solids (mass)Materials: Stainless steel

Nickel-based alloys, Titanium Rubber-lined steel Special materials

Filtering Surface: 2.0 m2

Max Speed: 1250 rpm

Weight: 3800 kg

Price: request pending - $150, 980 (Equation)

Vertical Centrifuge VZU

Broadbent IPD

Size: 260 x 160 mm

Capacity: 5.6 L

Materials: stainless steels Hastelloy Fluorocarbon

coatings

Filtering Surface: 0.13 m2

Max Speed: 3000 rpm

Centrifugal Factor: 1300 g

Deep Basket Centrifuge

Motor Power: 0.75 kW

Weight: 130 kg

Price: Request pending - $44,955 (Equation)

Recommendations

• Broadbent IPD Ideal performance for process Commonly used in related industry Smaller, more appropriate size than KMPT model

Centrifuge Recommendation:

Operation and Maintenance: • 2 installed in parallel (final purification step)

• Frequent overhaul and maintenance• Residence time dependant on inlet flowrates and

composition With given data, residence time = 3.9 hours

• Average cycle time: request pending Number of multiple cycles will depend on cycle times

Thank you.

Questions?