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bbe MOLDAENKE b iological • b iophysical • e ngineering THE ECOLOGICAL WATERSHED The bbe Cuvette Fluorometer – improved measurement in algae analysis in Laboratory and Field
bbeMOLDAENKE
b iological • b iophysical • engineering
T H E E C O L O G I C A L W A T E R S H E D
The bbe Cuvette Fluorometer –improved measurement
in algae analysis
in Laboratory and Field
The EXCEL spreadsheet shows an example of collected data obtai-ned with the bbe Cuvette Fluorometer. Water samples were takenfrom water surface at the same place off the shore of Lake Bistenseeduring July 1999. Data was stored in the internal data logger ofbbe Cuvette Fluorometer. Increasing sunlight with rising temperatu-res lead to enhanced growth of blue-green algae while concentra-tion of other groups was not largely affected. Developing bloomscan therefore be detected early on.
Dynamics of algae populationsin summer 1999 Lake Bistensee,Germany
Series of 4 identical samples:no pollutionsample 1: Toluenesample 2: Dichloromethanesample 3: Diuron
Follow the course of algae development
Algae Activity
Measurement of toxic compounds with the bbe Cuvette Fluorometer
0
1
2
3
4
5
27.07.25.07.23.07.21.07.19.07.17.07.15.07.13.07.11.07.09.07.07.07.
Cryptophyceae
Diatoms
Green Algae
Blue-green Algae
0
20
40
60
80
100
Sample 3Sample 2Sample 1No pollution
Determination of photosynthetic activity affected by the presence ofharmful herbicides is sensitively performed with the bbe CuvetteFluorometer activity measurement (optional)
Simoultaneous determination of Green, Blue-Green Algae,Diatoms and Dinophyceae, Cryptophyceae
Lake Bistensee Pos. 10908, 1999
Recognition of polluted samples
µg/l
Analyse your water sample using rapid fluorescence techniques
Simple handling – meaningful results
Complete chlorophyll analysis plus algae classdifferentiation in real time
The bbe Cuvette Fluorometer is a well established instrument for chlorophyllanalysis. For single measurements or measurement of collected water sam-ples the fluorometer provides prompt and reliable results of algae content.Based on fluorescence excitation spectra up to 5 different algae classes aredetermined simultaneously. No preceding treatment is needed. Measureddata is stored in the integral data logger or sent to a connected PC.
Excitation of algae by visible light results in fluorescence from chlorophylla, a pigment found in all photosynthetic reaction centres of algae andother photosynthetic organisms. Incidence of other pigments like phycocya-nin or fucoxanthine are typical for different algae classes. Interaction ofthese different pigment systems with a chlorophyll a core results in specificexcitation spectrum for a taxonomic related algae class. The specific pat-terns of algae fluorescence – the fingerprints – are used with the bbe soft-ware for quantification of the different classes. A set of 5 LED with selectedwavelengths are used for sequenced illumination. Fingerprints of newly intro-duced algae can be recorded for a more detailed analysis.
Optional information about photosynthetic activity can be achieved.Referring to the Genty-Parameter fo, f, fm fluorescence measurement in aprogrammed mode enables calculation of active chlorophyll under definedconditions. This measurement is comparable to measurement of oxygenevolution by photosynthetic active membranes. An optional toxicity test soft-ware is available for detection of herbicides.
Easy operation
The approved software developed for bbe Cuvette Fluorometer is used tocontrol the algae analyzer. Quantitative distribution of algae class are perma-nently processed and displayed. Collected data can be easily transferred toconnected PC via RS232 serial communications. Start, stop and operation ofthe Fluorometer is achieved via a simple membrane keypad on top of instru-ment. A selfexplanatory menu enables rapid access to all important functionsand operations.
Applications
River water controlDrinking water monitoringRiver dam controlContinuos supervision in monitoring stationLimnological and oceanographic researchEducationHerbicide pollution controlToxicity screeningPublic health protection against bluegreen algae blooms
bbeMOLDAENKE
@
Wildrosenweg 3D-24119 Kiel-KronshagenFon (+49) 431 38 04 0- 0Fax (+49) 431 38 04 0 -10E-Mail [email protected]://www.bbe-moldaenke.de
Features and benefits of the bbe Cuvette Fluorometer
Our Cuvette Fluorometer is merely one of an entire range of advancedinstruments for water quality control. Different tasks may require alternativeapproaches. For more details of specific solutions please contact us orvisit our website.
Rapid and reliable chlorophyll-determinationDetection and quantification of up to five algae classesPhotosynthesis activity measurement (optional)Transmission measurementReplacement of wet chemical analysisToxicity assessment (optional)Field use possible with optional battery
Brief Technical Data
For more information please visit our website
Your local bbe dealer
Technical data bbe Cuvette FluorometerMeasuring range 0.1 - 250 µg/lWeight 11 kgSize (h x w x d) 220 x 370 x400 mm3
Voltage 220 V / 12-14 V= Laser 12 V/20 WData capacity max. 1500Serial communications 1 x RS232Serialwater resistant housing IP 54
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