of 27
/27
1 2010 12 15 ( ) , ( , PWRI) Andrew JOHNSON (Centre for Ecology and Hydrology, CEH) 22
1
2010 12 15
( ), ( , PWRI)Andrew JOHNSON
(Centre for Ecology and Hydrology, CEH)
22
•
2005 2009•
• Roach
EDC
2
0 per cent
1–20 per cent
21–40 per cent
41–60 per cent
61–80 per cent
81–100 per cent
Jobling et al., 2006)
3
17�-estradiol (E2)
Estrone (E1)
OH
OH
O
OH
Ethynylestradiol EE2)
OH
OH
ng/L4
0
2
4
6
8
10
12
14
16
18
England Japan
Nor
mal
ised
exc
retio
n E
2 eq
uiv
ug/h
ead/
d
2%( , 2001)
%
E2 (Johnson et al., 2006
0.6 %
?
? ? ?
6
1)2)3)
UK-J Phase 2 CORE 1 (2005 to 2009)
Estradiol (E2) Estriol (E3) Estrone(E1)
( or
Ethynyl estradiol (EE2)
O
C ONa
OH
HOOH
OOH
SO
O
OH
OH
7
Sample
SPE
(GF/B)
Oasis HLB
STD
NH2 Cartridge
CH3CN/H2O(1:9),1ml
MeOH 0.5% NH4OH/MeOH
N2 Evap.
LC-MS/MS LC-MS/MS
Clean up
Clean-up with NH2
Change LC 50 mm 100 mm
: 500 ml / : 1000 ml
8
0
10
20
30
40
50
60
70
80
90
100
E1 E2 E3 EE2 E1-3S E2-3S E3-3S E1-3G E2-3G E3-3G
Con
cent
ratio
n (n
g/L)
Influent Effluent
UK (n = 3)
� E1, E2 EE2
�
�
=Range
9
� EE2� E1� E1
n=29
0
10
20
30
40
50
60
70
80
90
100
E1 E2 E3 E1-3S E2-3S E3-3S E1-3G E2-3G
Con
cent
ratio
n (n
g/L)
0
10
20
30
40
50
60
70
80
90
100
Rem
oval
effi
cien
cy (
%)
Influent Effluent Removal
10
0
20
40
60
80
100
E1 E2 E3 EE2 E1-3S E2-3S E3-3S E1-3G E2-3G E3-3G
Rem
oval efficien
cy (%
)
Removal efficiency
-80
-60
-40
-20
0
20
40
60
80
100
E1 E2 E3 EE2 E1-3S E2-3S E3-3S E1-3G E2-3G E3-3G
Rem
oval
effi
cien
cy (%
)E2,EE2
E1
Jap
an (
n=
9)U
K (
n=
3)
=Range
11
12
� E1 EE2E2
� EE2
0
10
20
30
40
50
Con
cent
ratio
n (n
g/L)
Influent
Effluent
Downstream
E1 E2 E3 EE2 E1-3S E2-3S E3-3S E2-3&17GE3-3&17G
Co
nce
ntra
tion
(ng
/L)
13
Benson Sandhurst
R. Thames Camberley
Parking
Sandhurst
14
E2?
PST Reactor FST
Qs
Qs Qs
PST Reactor FST
Qs
Qs
kQs
Qs
kQs
+
SRT
15
SRT=22E1 E2
�� ..
N=6
0
2
4
6
8
10
12
14
16
18
20
E1 E2 E3 E1-3S E2-3S E3-3S
Con
cent
ratio
n (n
g/L)
0
20
40
60
80
100
Rem
oval
effi
cien
cy (
%)
Secondary Effluent After Ozonation Removal
16
D7D4
D5
D3
D2
D9
D8D6
D1
U2
U1
K1
U9 U6
U7
U9U10
K7
K9K8
K6K5
K10
K4K2
K3
U3 U4
U9
17
�E2 EE2 , E1�E1 ,�E1
(segment)
(segment)
STP
STP
STP
60
, , ,
18
0
1
2
3
4
5
6
7
8
9
10
Fro
me
Dar
t
Tor
ridge
Cuc
kmer
e
Gre
at S
tour
Aru
n
Soa
r
Tem
e
Cro
uch
Cam
Wea
r
Tee
s
Ede
n
Cal
der
Wyr
e
Taf
f
Tyw
i
Eas
tern
Cle
ddau
Con
wy
Mer
sey
Irwel
l
Aire
Don
Bly
th
Ere
was
h
Tre
nt
Idle
Littl
e O
use
Wav
eney
Tha
mes
Med
way
Avo
n
0
1
2
3
4
5
6
7
8
9
1075% 25%
19
EEQ (ng/L) E
stim
ated
EE
QE
2-eq
uiv
alen
t,n
g/L
ng/L
Risk Class
No risk
At risk
High risk
LF2000-WQX
(>1 ng/L EEQ)
(>10 ng/L EEQ)20
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0R
umoi
Sar
u
Tok
oro
Ishi
kari
Aka
Yon
esiro
Kita
kam
i
Tsu
rum
i
Kuj
i
Nak
a
Kak
ehas
hi
Kur
obe
Ara
Shi
nano
Kik
u
Abe
Syo
unai
Kis
o
Kita
Yam
ato
Yur
a
Ash
ida
Asa
hi
Gou
no
Dok
i
Mon
obe
Niy
odo
Yos
hino
Hom
myo
Kim
otsu
ki
Ooi
ta
Chi
kugo
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
1ng/L
30.6 18.0
98Summer
98Autumn
99Spring
99Summer
99Autumn
99Winter
00Autumn
01Autumn
NA NA NA NA NA
NA:No available data
21
Est
rog
enic
Act
ivit
ies,
E2-
equ
ival
ent,
ng
/LEEQ
(ng/L)
YES Assayw/ hER
22
•• k=0.067 /h
0
1
2
3
4
0 1 2 3 4
Estimated Concentration (ng/L)
Mea
sure
d C
once
ntra
tion
(ng/
L)
Theoretical line (x=y)
23
24
� SRT E1 , O3.
25
SRT + O3
E1
25
• EE2E2
• SRT
•
•
•
Summary
26
27
Acknowledgements
12th Annual Scientific Workshop UK-Japan Co-operation for Research on Endocrine Disrupters in the Aquatic Environment held at Otterburn HallNorthumberlandNational Park UK on 2-4 November 2010
