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Collect Environmental Sample Collect Lysate from Plate with Highest Plaque Density Spot Titer Full Plate Titer Prepare Webbed Plates Plaque Assay Pick a Plaque & Resuspend Serially Dilute Sample with Phage Extract Phage with SMALL Volume of Media Spin & Filter to Remove Particulate & Bacteria Test for Phage by Plaque Assay Extract Phage with LARGE Volume of Media Spin & Filter to Remove Particulate & Bacteria Add Host Bacteria & Incubate Spin to Remove Host Bacteria Test for Phage by Spot Test Collect Lysate from Webbed Plates Spot Titer Full Plate Titer ? Sample Contains Phage? ? Plaque Morphologies Consistent? ? Titer > 10 pfu/ml? 9 ISOLATION PURIFICATION Image by Electron Microscopy MICROSCOPY AMPLIFICATION Compare Phages by Restriction Digest of DNA & Gel Electrophoresis Extract Phage DNA EXTRACTION CHARACTERIZATION Archive & Create phagesDB Entry
