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Bioinformatics National Certification Examination:2013

Paper-I

Instituted By

Department of Biotechnology

Government of India

New Delhi

No of questions:100 Marks :100*1=100 Duration:2 Hours NOTE:- there will be negative marking of 0.25 for every wrong answer. Call me:08940080293 Send me mail:naveenbioinformatics@gmail.com

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YYoouu ccaann ddoo aannyytthhiinngg,, bbuutt nnoott eevveerryytthhiinngg..

——DDaavviidd AAlllleenn

Q(1): AAComp (Ident,sim) is use for find sequences in which databases? (a)Swiss-Prot (b)Trembl (c)CATH (d)PDB Q(2):Choose the correct format for ACCESSION NUMBER? (a)4-6 digits followed by 1-2 letters (b)2-4 digits followed by 1-2 letters (c)4-6 digits followed by 4-6 letters (d)1-2 letters followed by 4-6 digits Q(3):BLASTN and BLASTP are the version of BLAST that searches:- (a)a nucleotide sequence against a nucleotide database and a protein sequence -against a protein database respectively (b) a protein sequence against a protein database and a nucleotide sequence -against a nucleotide database respectively Q(4):choose True/False:- BLOCKS is a collection of blocks; a block represents:- (i)a motif with some structural or functional significance. Q(5):CATH database classifies domain structures in PDB.Each structure is classified at four levels:- (a)<class>,<Architecture>,<Topology>,<Genus> (b)<order>,<Genus>,<Class>,<Architecture>

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(c)<Homologous superfamily>,<Heterologous superfamily>,<Class>,<Architecture> (d)<Class>,<Architecture>,<Topology>,<Superfamily> Q(6):OWL is a composite database of: (a)Swiss-prot, PIR and Metacyc (b)Swiss-prot,PIR and Ecocyc (c)Swiss-prot,PIR,Genebank and NRL-3D (D)Swissprot,tremble,Metacyc Q(7):TransFac is a Secondary database which have information about:- (a)eukaryotic transcription Factors (b)Prokaryotic transcription Factors (c)both a & b (d)none of the above

Perfection is achieved, not when there is nothing more to add,

but when there is nothing left to take away. —Antoine de Saint-Expiry

Q(8)Choose the correct pair of databases which is belong to primary database? (a)Genebank,EMBL,NDB,CATH (b)GeneBank,EMBL,NDB,PDB (c)GeneBank,EMBL,PDB,KEGG (d)GeneBank,EMBL,NDB,SCOP Q(9) Choose the correct pair of databases which is belong to secondary database? (a)SCOP,CATH,PRODOM,NDB (b)SCOP,CATH,PRODOM,OMIM (c)SCOP,Pfam,Prosite,NDB (d)SCOP,CATH,Transfac,PDB Q(10)Choose the correct statement from the following:- (a)BLOSUM tends to give higher scores to substitutions involving hydrophilic aminoacids and lower scores to substitutions involving hydrophobic aminoacids.

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(b)BLOSUM tends to give higher scores to substitutions involving hydrophobic aminoacids and lower scores to substitutions involving hydrophilic aminoacids. Q(11) STRING is a search tool for:- (a)The Retrieval of interacting DNA only (b)The Retrieval of interacting protein only (c)predict the DNA-DNA interaction (d)The Retrieval of interacting Genes/Proteins Q(12)Orthologuous are: (a)proteins that have the same function in different species (b)genes that have arisen from a common ancestor (c)genes that may or may not have arisen from a common ancestor (d)both a and c are correct Q(13)choose the correct statement for Needleman-Wunsch algorithm:- (a)it’s a local alignments (b)Residue alignment score may be positive or negative (c) Residue alignment score for a pair of residues to be >=0 (d)Requires a gap penalty to work effectively Q(14)alpha helix has a pitch(in armstrong) of:- (a)5.4 (b)6.0 (c)4.7 (d)4.9

Courage is not the absence of fear, but rather the judgement that something else is more important than fear.

—Ambrose Redmond

Q(15)Signalp is a software tool which is useful for:- (a)Prediction of coiled coiled structures of proteins (b)Neural Network prediction and location of signal peptide cleavage sites in amino acid sequences from different organisms (c)signal sequence based prediction of location and using stastistics and similarity matrices (d)both a and c

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Q(16)Exon suffling is the process by which :- (a)exons are duplicated in the same DNA molecule (b)exons are duplicated in the different DNA molecule (c)exons are removed in the different dna molecule (D)exons are deleted in the same DNA molecule Q(17)16s Ribosomal RNA sequence are widely acceptable as phylogenetic marker,because:- (p)These sequences exit in all organism (q)They are highly conserved across kingdoms (r)These sequences are long sequences which are widely sequenced (s)The sequencing of these sequence is easy Choose the correct option:- ((aa))pp,,qq ((bb))pp,,qq && rr ((cc))qq && rr ((dd))pp,,qq,,rr &&ss Q(18)The Genebank Flat File Format is divided into:- (a)<Header> <sequence> <Footer> (b)<Header> <Sequence> (c)<Header> <Features> <Footer> (d)<Header> <Features> <Sequence> Q(19)An optional ‘*’ at the end of the file indicates end of sequence,which is used in:- (a)GeneBank Flat-File Format (b)FASTA Format (c)FASTA and GCG Format (d)FASTA and GCG-MSF Format

You must be the change you wish to see in the world. —Gandhi

Q(20)In The sequence part of the GenBank Flat-File Format:- (a)bases are placed in blocks of 8 bases and each line of sequence has 6 blocks (b)bases are placed in blocks of 7 bases and each line of sequence has 7 blocks

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(c)bases are placed in blocks of 10 bases and each line of sequence has 6 Blocks (d)bases are placed in blocks of 6 bases and each line of sequence has 10 blocks Q(21)BRENDA is a :- (a)Structure database (b)Microarray database (c)Chemical database (d)Pathway Database Q(22)Select the databases which are belong to structure databases? (a)FSSP,DALI (b)BRENDA,KEGG (c)ArrayExpress and MIAME (d)SP-ML, NEWT Q(23)Cladogram depict the :- (a)amount of evolutionary change that has occurred along the different branches. (b)Relative recentness of a common ancestry (c)times of divergence (d)both b & c Q(24)The number of rooted trees(Nr) for ‘n’ OTUs (Operational taxonomic unit) is given by:- (a)Nr = (2n-5) ! / 2n-2(n-2)! (b) (2n-3) ! / 2n-2(n-3)! (c) (2n-3) ! / 2n-2(n-2)! (d) (2n-5) ! / 2n-3(n-3)! Q(25)Haemoglobin has quaternary structure due to association of:- (a)4 alpha globin polyproteins (b)2 alpha globin and 2 beta globin polyproteins (c)1 alpha- globin and 3 beta globin polyproteins (d)4 alpha globin and 4 beta globin

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When hungry, eat your rice; when tired, close your eyes. Fools may laugh at me, but wise men will know what I mean.

—Lin-Chi Q(26)3’ UTR:- (a)The sequence at the 5’ end of rRNA that code for product (b)The Sequence at the 3’ end of rRNA.that doesnot code for Product (c)The sequence at the 3’ end of messenger RNA that doesnot code for the product (d)The sequence at the 3’ end of messenger RNA that code for the product Q(27)DUST ( A program for filtering low complexity Regions from the:- (a)amino acid Sequences (b)Nucleic acid Sequences (c)Nucleic and amino acid sequences (d)amino acid of BETA-pleated sheet Q(28)Homology is the similarity in DNA or protein sequences between individuals of the:- (a)Same species (b)different species (c)same or among different species (d)none of the above Q(29)The ExPASy is dedicated to the analysis of protein sequences and structures as well as 2-D PAGE.This server is maintained by--------------- (a)Swiss Institute of Bioinformatics (b)European Bioinformatics Institute (c)The Institute of Genome Research (d)Protein Information Resource Q(30)International standard Organization for describing structural data is------- (a)PDB (b)ASNI (c)CATH (d)none of these

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Q(31)At the NCBI website ,the default scoring matrix for protein-protein BLAST analyses(BLASTP) is the BLOSUM62 matrix.The most likely reason for this matrix to be selected by default is because---- (a)it represents a compromise between information content for each residue and the amount of information that contributes to the matrix (b)only this matrix is appropriate for all protein comparisons (c)it incorporates 62 position –specific scoring patterns (d)unlike PAM matrices,this matrix is based on explicit phylogenetic information The third-rate mind is only happy when it is thinking with the

majority. The second-rate mind is only happy when it is thinking with the minority. The first-rate mind is only happy

when it is thinking. —A. A. Milne

Q(32)KITSCH, a package in PHYLIP is based on----- (a)Maximum Likelihood method (b)distance method (c)Maximum Parsimony method (d)none of these Q(33)Maximum Likelihood method is used in molecular phylogeny when-------- (a)there is maximum similarity among the aligned sequences (b)there is minimum similarity among the aligned sequences (c)there is maximum number of hits found in BLAST (d)all the sequences are from same phylum Q(34)Within the Greek Key Motif there are---- (a)2 antiparallel beta sheets and one coil (b)2 pair of antiparallel beta sheets joined by a coil (c)2 parallel beta sheets,one alpha helix,and two coils (d)none of these

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Q(35)The software that takes file input in mmdb format is--- (a)Cn3D (b)RasMol (c)SPDV (d)PymoL Q(36)The first protein to be sequenced is ----- (a)insulin (b)haemoglobin (c)myosin (d)tannin Q(38)The server that provides data about quarternary structure of the proteins is--- (a)PDB (b)PQS (c)SRS (d)E-MSD Q(39)Cn3D is a--------- (a)molecular file format (b)molecular viewer (c)molecular file format conversion tool (d)all of these To the man who only has a hammer, everything he encounters

begins to look like a nail. —Abraham Maslow

Q(40)The matrix used for the best local alignment of protein sequence is---- (a)BLOSUM62 (b)IUB (c)BLOSUM80 (d)PAM20 Q(41)The ribosome is involved in all of the following,except (a)peptide bond formation (b)aminoacylation of trna (c)binding of protein factors during elongation (d)binding of aminoacyl trna to mrna

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Q(42)Which of the following is not directly associated with regulation of eukaryotic gene expression? (a)acetylation of histones (b)methylation of DNA (c)alternative splicing (d)activation of caspases Q(43)DNA polymerase contains a lysine residue that is important for binding to DNA.Mutations were found that converted this lysine to either glutamate,glycine,valine or arginine.Which mutations would be predicted to be the most and least harmful to the ability of the enzyme to bind DNA/ Most Least a.glycine arginine b.arginine glycine c.glutamate valine d.glutamate arginine Q(44)Which of the following is not true about alanine :- (a)at pH 1,the overall charge is +1 (b)at pH 1,it will move towards cathode (c)an equimolar mixture of D and L alanine does not rotate the plane polarized light (d)contains branched side chain Q(45)Match the transition state or chemical entity of each enzyme that is responsible for their catalytic function:- Column I Column II P. ribonuclease 1. oxyanion Q. lysozyme 2. pentacovalent phosphorus R .Chymotrypsin 3.carbonium ion S. Carboxypeptidase 4. mixed anhydride

a. P-3,Q-2,R-4,S-1 b. P-2, Q-3,R-1,S-4 c. P-2,Q-1,R-3,S-4 d. P-4,Q-3,R-2,S-1

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Q(46)In the pentose phosphate pathway (a)only the C-1 carbon of glucose is oxidized to CO2 (b)all the carbons of glucose is oxidized to CO2 (c)no decarboxylation occurs (d)C-4 and C-5 of glucose is oxidized to CO2 Q(47)Shell scripting is mostly used to: (a)Program operating system of Windows,UNIX,etc. (b)Program command line of an operating system (c)All of above (c)None of above Q(48)The slow executing speed of shell can be resolved by: (a)Echo command (b)Environmental variables (c)Exit command (d)Using pipeline and filter commands Q(49)Abstraction in OOP enables: (a)The class to get its specific class of inheritance (b)Different classes and objects to send messages to each other (c)a sub class to act like a parent class (d)None of above Q(50)UML lacks implementation of polymorphism on message arguments: (a)False (b)True Q(51)Parametric polymorphism can be used: (a)Only once (c)Only twice (c)Thrice (d)Any number of times Q(52)Magic table in SQL is: (a)Primary and Foreign Key (b)Insert and Delete Commands (c)Stored procedures (d)DDL

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Q(53)Run Time Memory Allocation is: (a)Static memory allocation (b)Physical memory allocation (c)Dynamically allocating memory (d)Virtual memory allocation Q(54)After a meal, blood glucose enters cells and is stored as glycogen,particularly in the liver.Which of the following is the donor of new glucose molecules in glycogen?

a. UDP-glucose-1-phosphate b. UDP-glucose c. UDP-glucose-6-phosphate d. Glucose-6-phosphate

Q(55)The beta-oxidation of a molecule of palmitic acid:- (a)Yields 8 molecules of acetyl-coA only (b)Yields 16 molecules of acetyl-coA only (c)Yields carbon dioxide and water only (d)does not involve oxygen Q(56)Which one of the following characteristics best applies to an allosteric effector? (a)competes with substrate for the catalytic site (b)binds to a site on the enzyme molecule distinct from the catalytic site (c)changes the nature of the product formed (d)changes the substrate specificity of the enzyme Q(57)In the pentose phosphate pathway (a)only the C-1 carbon of the glucose is oxidized to CO2 (b)all the carbons of glucose is oxidized to CO2 (c)no decarboxylation occurs (d)C-4 and C-5 of glucose is oxidized to CO2 Q(58)In C4 plants, phosphoenol pyruvate carboxylase is located in (a)cytosol (b)chloroplast (c)peroxisome (d)mitochondrion

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Q(59)IP3 initially causes Ca2+ to be released into cytoplasm from: (a)mitochondria (b)lysosome (c)the endoplasmic reticulum (d)the plasma membrane(from extracellular to intracellular) Q(60)In a database table the category of information is called:- (a)Tuple (b)Field (c)Record (d)All of above Q(61)This key uniquely identifies each record (a)Primary key (b)Key record (c)unique key (d)field name Q(62)the Database language that allow you to access or maintain data in a database? (a)DCL (b)DML (c)DDL (d))none of the above Q(63)what punctuation is used to signal the beginning and end of code Blocks? (a){} (b)-> and <- (c)begin and end (d)(and) Q(64)The capacity of 3.5 inch floppy disk is? (a)1.40 Mb (b)1.44 GB (c)1.40 GB (d)1.44 MB

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Q(65)Which of the following transmission systems provides the highest data rate to in individual device? (a)computer bus (b)telephone lines (c)voice and model (d)lease lines Q(66)A collection of 8 bits is called (a)byte (b)word (c)record (d)none of the above Q(67)A stack is (a)an 8-bit –register in the microprocessor (b)a 16-bit register in the microprocessor (c)a set of memory locations in R/WM reserved for storing information temporarily during the execution of computer (d)both a & b Q(68)In OSI network architecture,the dialogue control and token management are responsibility of:- (a)session layer (b)network layer (c)transport layer (d)data link layer (e)all the above Q(69)Which command divides the surface of the blank disk into stors and assign a unique address to each one:? (a)ver (b)format (c)fat (d)chkdsk

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Q(70)Each time you turn on your computer,it will check on the control file? (a)command.com,io.sys (b)command.com,date.com,dir.com (c)command.com,io.sys,msdos.sys (d)chk dsk.exe Q(71)select the mean and the median for: (88,72,33,29,70,54,86,91,57,61) (a)64.1,65.5 (b)65.5,64.1 (c)60.2,61.5 (d)61.5,60.2 Q(72)A box contains 4 white and 6 black balls,if 3 balls are drawn at random,find the mathematical expectation of the number of white balls:- (a)1.2 (b)2.3 (c)1.9 (d)2.4 Q(73)Which of the following are intensive properties? 1.Kinetic Energy 2.Specific Enthalpy 3.Pressure 4.Entropy (a)1 and 3 (b)2 and 3 (c)1,3 and 4 (d)2 and 4 Q(74)Two cables are attached to an old telephone pole at right angles to each other.Each is pulled with a force of 5000N.what is the net force on the pole exerted by the two cables? (a)7071N (b)6789N (c)8397N (d)4750N (e)50 million N

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Q(75)Which of the following limits the application of Newtonian laws of physics?

(a) the positions of all the atoms in the universe (b) gravity (c) chaos (d) quantum mechanics (e) both c and d

Q(76)Which of the following molecules does not have a net dipole moment? (a) H2O (b) NH3 (c) BF3 (d) BrF5 Q(77)Which of the following hydrocarbons does not have isomers? (a) C7H16 (b) C6H14 (c) C5H10 (d) C4H8 (e) C3H8 Q(78)Which one of the following compounds is an isomer of CH3CH2CH2CH2OH? (a) CH3CH2CH2OH (b) CH3CH(OH)CH3 (c) CH3CH2CH2CHO (Note: This is one way to write an aldehyde.) (d) CH3CH2CH2CH3 (e) none of the above

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Q(79)If the temperature of a reaction is increased by 20oC, the reaction rate will be... (a) 2 times as fast. (b) 4 times as fast. (c) 20 times as fast. (d) unchanged because the reaction rate is not dependent the temperature. Q(80) When the following reaction equation is properly balanced, the number of moles of O2 will be... (a) 1.5 moles (b) 3.5 moles (c) 3 moles (d) 5 moles Q(81) When were the first minicomputer built? (a)1965 (b)1962 (c)1971 (d)1966 Q(82)C is which level language? (a)HLL (b)MLL (c)LLL (d)DLL Q(83) In C language reading data from keyboard? (a)Printf (b)Scanf (c)Int (d)Main()

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Q(84)The first network database is? (a) Ingress (b)DBTG Codasy1 (c)Database1 (d)SAP Q(85)The UNIX operating system is written in language? (a) Cobol (b) C Language (c)Binary (d)Fortran Q(86) The function of a kernel in an operating system is to? (a) Interpret the JCL commands (b)Interact with the hardware device (c )Intract with the user (d)None of above Q(87)The techenique, which stores a program on a disk and then,transfers the program into main memory, as and when the are needed, is known as? (a)Thrashing (b)Swapping (c)Spooling (d) None of above

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Q(88) The slowest transmission speeds are these of? (a) Twisted ?pair wire (b)Coaxial cable (c)Fiber ? optics cable (d) Microwaves Q(89)Which escape character can be used to new line in C? (a) `(a)` (b)`()` (c) `(v)` (d) `(k)` Q(90) Which of the following items is not a UTP cable? (a) CAT2 (b) CAT3 (c) CAT100 (d)CAT5

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Q(91)Express (7 + 3i) - (2 - 3i) in the form a + bi where a and b are real numbers. (a) 5 - 6i (b) 5 + 6i (c) 9 (d) 9 + 5i Q(92)Find the distance d(A, B) between the points A(−1, 0) and B(4,3):- (a) 3√ 2 (b) 5 (c)√34 (d) 8 Q(93)Give the equation for the circle with center C(3, −2) and radius 4. (a) x2 + y 2 = 52 (b) (x − 3)2 + ( y − 2)2 = 16 (c) (x + 3)2 + ( y − 2)2 = 42 (d) (x − 3)2 + ( y + 2)2 = 16 Q(94)Water molecules dissociate producing hydrogen ions (H+), and hydroxide ions (OH-). Regarding the ion product of water, all of the following statements are true EXCEPT: (a) At equilibrium, the product of the concentrations of the dissociated ions divided by the concentration of the undissociated water is a constant, K (b) In an aqueous solution the concentrations of hydrogen ions and hydroxide are inversely proportional (c) Because the extent of dissociation is very small, the concentration of pure water remains essentially at 55.5 M (d) Kw is a constant representing the ion product of water and is equivalent to = 1 x 10-14. (e) If H+ is added to an aqueous solution, OH- increases proportionately

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Q(95)The pH of a blood sample with an [H+] of 40 x 10-9 Eq/L is (a)6.4 (b)5.3 (c)7.4 (d)8.4 (e)4.9 Q(96) Which statement is false? A sigma molecular orbital a. may result from side-on overlap of p atomic orbitals. b. may result from head-on overlap of p atomic orbitals. c. may result from overlap of an s atomic orbital with a p atomic orbital. d. appears spherical when viewed along its major axis (axis of overlap). e. may be either bonding or antibonding. Q(97) The molecular orbital energy level diagram has the σ2p orbital at a lower energy level that the two !2p orbitals for which of the following molecules? (a) C2 (b) N2 (c)B2 (d) O2 (e) Li2

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Q(98)The electrode at which oxidation occurs is called the (a) reducing agent (b) anode (c) cathode (d) voltaic cell (e) oxidizing agent Q(99)50 m3 of a gas at 298 K is heated to 322 K at constant pressure. What is the new volume of gas? (a) 0.022 m3 (b)1900 m3 (c)46 m3 (d)54 m3 Q(100)If ΔGo(298 K) for a reaction is –8.5 kJ mol–1, what is the corresponding value of the equilibrium constant K? (a) 0.032 (b)1.0 (c)3.4 (d)31 NOTE:-This is just a sample paper of BINC-I paper, which is written for giving some idea about what type of question they used to asked in BINC- exam, (in previous years).....by N@veeN-KumaR-MeenA