Bio-Luminescence Detection

Project Manager: Eitan NovotnySean Dang

Yu-Kun ZhangPhilip Tran

Overview

• Biological rhythms– Samples emit periodic light– Periods around 24 hours– Sample rate once every 10 minutes

• Commercial device flaws– Missing flow through function– Product is costly

• Design goals– Integration with original equipment– Improve function and reduce cost

• MDR Specification• Design Changes

Box Design

• 39cmx39cmx32cm• Designed in Pro

Engineering• Two Pieces

– Main box– Lid (top and front)

Box Design - Details

• Internal temperature must match incubator

• Two 80mm square holes plus one 80mm fan for airflow

• Light isolated environment

• 5 BNC connectors for PMTs and Motor

• 2 18cmx4cm windows for tube baffles

Box Design - Overview

• Light baffle

Main Box

Lid

Motor

• 1240i board works with specific motors

• Parallel motor connection not possible

• Principal needs– Single high torque motor– Four rotating mirrors

Motor - Solution

• Motor is Oriental Motor PK243A1A-SG18– Used in commercial application

• Step up gear used for high precision

• Central gear and outer gear are 5”

• Shafts connect outer gears to mirrors

• Contact switches used for error checking

Light Source/Detector

• Luciferin - emits light• Wavelength range: 268nm

to 537nm• Photomultiplier Tube

(PMT)• Detects wavelength range

from 185nm to 680nm• Effective area 4mm x

20mm

Light Efficiency/Path Length

• Commercial application has a .25 cm path length

• Our light path length is about 3cm to 5cm

• Light goes to the mirror and reflects up to the PMT

• Loss of light due to vertical arrangement

• To minimize loss of light, add mirrors

Light Efficiency - Continued

• Interference from other light sources

• Add reflective walls, to minimize interference from other light sources

Electronics

• H8259 from Hamamatsu

• PCI-6601 Counter Board

• Applied Motion 1240i

• Oriental Stepper Motor

• Computer and software

BudgetPart Cost (each) Qty Price

• H8259 $1150 4 $4600• 1240i $200 1 $200• PCI-6601 $600 1 $600• Motor $177 1 $177• Metal $46.11 6 $277• Gears $62.02 5 $310• Shaft Holder $27.46 5 $137• Shaft $20.05 1 $20• Wires $100 $100

Total* $6421*total cost to date

Conclusion

• MDR Specification done before 2nd semester

• Accomplishments– Gearing design done– Box design done

• Mirror design will be completed before conclusion of semester

• Device should be completed on time

• Device should be under budgetAluminum for box construction