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Luisa Martins

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Rubens Maciel Filho

School o Chemical Engineering State University of Campinas

Modeling of Chemical and Biochemical Process: Computer AidedDesign, Computer Aided Process Engineering, Computer Aided

Process Operation Optimization of Chemical and Biochemical Process: Optimization

of Large Scale System, Off-Line Optimization, On-LineOptimization, Real-Time Process Integration.

Advanced Control Algorithms: Advanced Process Control, High

Quality and Performance Process Operation, Soft Sensors, Real-Time Process Integration

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Simultaneous acetone butanol ethanol(ABE) fermentation by C. beijerinckii P260

and in situ product recovery

Vacuum process operating in two modes:

Continuous

Intermittent: 1.5 h vacuum with intervals of 4, 6and 8 h

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Controlbatch

fermentation

Incomplete glucose utilization

Butanol Toxicity (13g/L in a batchfermentation)

In Situ

Recovery

Complete glucose utilization Greater productivity

Improved cell growth Concentrated recovered ABE stream

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Vacuum Process Recovery

14.4% to 77.0% of recovery

Presence of fermentation

intermediates Vacuum recovery time

Initial concentration of butanol

Removal rate had to higher

than ABE productivity

2 h to recover 68.5%

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ABE in Batch Bioreactor(Control)

Maximum cell growth at12 h

Residual glucose of 13.9g/L

60 h batch

Produced:

3.7 g/L acetone 11.8 g/L butanol

0.6 g/L ethanol

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ABE by Vacuum Fermentation

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18 h fermentation before vacuum process started

ABE yield is lower

Higher butanol concentration recovered

Continuous: 13.3 g/L 4 h: 35.9 g/L

6 h: 41.4 g/L

8 h: 36.5 g/L

Residual ABE in reactor for intermittent (18%)

C. beijerinckii P260is not adversely affected by thevacuum recovery

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Vacuum technology can be used to recover butanol

Enhanced performance of ABE fermentation due toremovel of butanol

Vacuum fermentation resulted in: Decrease in fermentation time Complete utilization of glucose

Greater cell growth

Concentrated product stream

Intermittent mode is desirable

lower energy costs andhigher ABE concentrations

Best experimental situation: 1.5 h sessions in 4 hintervals 3.7 higher butanol concentration

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Attempt to make biofuel production more attractive

Concerns about productivity

Higher concentrations will lead to less costs in

purification Cost analysis is it worth?

Loss control in vacuum tubing and pumps

Comparison to other used methods (gas stripping)

Using a mutant strain that is more tolerant to butanol C. beijerinckii BA101