Alzheimers DiseaseMost common cause of dementia in older populations34.4 million in 2009 Late Onset Alzheimers Disease appears after ! "ears of a#e Lifest"le$ %n&ironmental$ 'enetic factors'enetic (is) *actors Apolipoprotein % +4 allele , A-O% rs4293!. and rs/402 1230 rs/443/3 4L5 rs0003000 A14A/ rs3/4!0 4(0 rs3.0.30 -24ALM rs3.!00/9 M64AA rs00932 4D33 rs3.!444$ M64A4% rs/0039 4D2A- rs934940/Allele speci7c pol"merase chain reactionthe speci7c primers are desi#ned to permit ampli7cation 8" D3A pol"merase onl" if the nucleotide at the 3,end of the primer perfectl" complements the 8ase at the &ariant or 9ild,t"pe se:uences. relati&el" cheaper than other a&aila8le methods -4( restriction fra#ment len#th pol"morphism -"rose:uencin# pro8e h"8ridization 8ased techni:uesO8;ecti&esde&elop$ &alidate and utilize an A6,-4( method to determine 63-s in the ne.Materials and Methods%thics appro&alD3A samplin# memor" and #eneral #eriatric patients of 5ni&ersit" of Mala"a Medical 4entre in Mala"sia from ?ul" of 2000 to ?une of 2002 'enot"pin# test primers desi#ned usin# Oli#o % and re&erse common =(c> primer of each #ene !0 n# of #enomic D3A for the ampli7cation of each #ene6econd (ound -4( 2 BL of a 0C!0 dilution of the first round -4( mi and re&erse allele,speci7c primer =(as>A6,-4( for 1230 rs/443/3 and 4L5 rs0003000initial denaturation at .0D4 for ! minutes3! c"cles of ampli7cation 944 for 0 min 3 D4 for 30 s and /2 D4 for 4 s/2D4 for ! min.