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“Biofiltration of Shrimp Pond Effluent by Oysters in a Raceway System”

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“Biofiltration of Shrimp Pond Effluent by Oysters in a Raceway System” Adrian B. Jones* and Nigel P. Preston C.S.I.R.O. Division of Fisheries Moreton Bay Prawn Farm Research funded by the Fisheries Research and Development Corporation. Saccostrea commercialis. Oyster’s Filter Feeding. - PowerPoint PPT Presentation
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Biofiltration of Shrimp Pond Biofiltration of Shrimp Pond Effluent by Oysters in a Effluent by Oysters in a Raceway System” Raceway System” Adrian B. Jones* and Nigel P. Preston Adrian B. Jones* and Nigel P. Preston C.S.I.R.O. Division of Fisheries C.S.I.R.O. Division of Fisheries Moreton Bay Prawn Farm Moreton Bay Prawn Farm Research funded by the Research funded by the Fisheries Research and Development Corporation Fisheries Research and Development Corporation Saccostrea Saccostrea commercialis commercialis
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Page 1: “Biofiltration of Shrimp Pond Effluent by Oysters in a Raceway System”

““Biofiltration of Shrimp Pond Effluent Biofiltration of Shrimp Pond Effluent by Oysters in a Raceway System”by Oysters in a Raceway System”

Adrian B. Jones* and Nigel P. PrestonAdrian B. Jones* and Nigel P. Preston

C.S.I.R.O. Division of Fisheries C.S.I.R.O. Division of Fisheries

Moreton Bay Prawn FarmMoreton Bay Prawn Farm

Research funded by the Research funded by the

Fisheries Research and Development CorporationFisheries Research and Development Corporation

““Biofiltration of Shrimp Pond Effluent Biofiltration of Shrimp Pond Effluent by Oysters in a Raceway System”by Oysters in a Raceway System”

Adrian B. Jones* and Nigel P. PrestonAdrian B. Jones* and Nigel P. Preston

C.S.I.R.O. Division of Fisheries C.S.I.R.O. Division of Fisheries

Moreton Bay Prawn FarmMoreton Bay Prawn Farm

Research funded by the Research funded by the

Fisheries Research and Development CorporationFisheries Research and Development Corporation

SaccostreaSaccostreacommercialiscommercialisSaccostreaSaccostreacommercialiscommercialis

Page 2: “Biofiltration of Shrimp Pond Effluent by Oysters in a Raceway System”

Oyster’s Filter FeedingOyster’s Filter Feeding

Oysters filter bacteria and phytoplankton and convert them to meat.Oysters filter bacteria and phytoplankton and convert them to meat.

Oysters filter inorganic material and pellet smaller particles into larger Oysters filter inorganic material and pellet smaller particles into larger pseudofacaes which can settle out of suspension.pseudofacaes which can settle out of suspension.

Oysters filter bacteria and phytoplankton and convert them to meat.Oysters filter bacteria and phytoplankton and convert them to meat.

Oysters filter inorganic material and pellet smaller particles into larger Oysters filter inorganic material and pellet smaller particles into larger pseudofacaes which can settle out of suspension.pseudofacaes which can settle out of suspension.

- Bacteria- Bacteria- Phytoplankton- Phytoplankton- Inorganic particles- Inorganic particles- Detritus- Detritus

- Bacteria- Bacteria- Phytoplankton- Phytoplankton- Inorganic particles- Inorganic particles- Detritus- Detritus

- Faeces- Faeces (organic) and (organic) and AmmoniaAmmonia- Psuedofaeces (inorganic)- Psuedofaeces (inorganic)

- Faeces- Faeces (organic) and (organic) and AmmoniaAmmonia- Psuedofaeces (inorganic)- Psuedofaeces (inorganic)

AnusAnusAnusAnusHeartHeartHeartHeart

IntestineIntestineIntestineIntestine

StomachStomachStomachStomach

MantleMantleMantleMantleGillsGillsGillsGills

Adductor Adductor MuscleMuscleAdductor Adductor MuscleMuscle

Style SacStyle SacStyle SacStyle Sac Labial Palps Labial Palps (mouth)(mouth)Labial Palps Labial Palps (mouth)(mouth)

HingeHingeHingeHinge

Page 3: “Biofiltration of Shrimp Pond Effluent by Oysters in a Raceway System”

Study AreaStudy Area

RedcliffeRedcliffeRedcliffeRedcliffe

BrisbaneBrisbaneCityCityBrisbaneBrisbaneCityCity

MoretonMoretonIslandIslandMoretonMoretonIslandIsland

NorthNorthNorthNorth

StradbrokeStradbrokeStradbrokeStradbroke

IslandIslandIslandIsland

MoretonMoretonMoretonMoreton

BayBayBayBay

BrisbaneBrisbaneBrisbaneBrisbane

Moreton BayMoreton Bay Prawn FarmPrawn FarmMoreton BayMoreton Bay Prawn FarmPrawn Farm

Page 4: “Biofiltration of Shrimp Pond Effluent by Oysters in a Raceway System”

Moreton Bay Prawn FarmMoreton Bay Prawn Farm

Page 5: “Biofiltration of Shrimp Pond Effluent by Oysters in a Raceway System”

Need for ResearchNeed for Research

Need to recapture some of the nutrients from the high cost feed pellets which are not Need to recapture some of the nutrients from the high cost feed pellets which are not converted into prawn biomass.converted into prawn biomass.

Prawn farm effluent contains elevated concentrations of bacteria, phytoplankton, Prawn farm effluent contains elevated concentrations of bacteria, phytoplankton, nutrients and suspended solids, which can potentially adversely affect the water quality nutrients and suspended solids, which can potentially adversely affect the water quality in the receiving waters.in the receiving waters.

Sewage treatment techniques are often ineffective due to the low specific gravity of Sewage treatment techniques are often ineffective due to the low specific gravity of most of the effluent particles, and the high volume and salt content. They are also most of the effluent particles, and the high volume and salt content. They are also prohibitively expensive.prohibitively expensive.

To develop a system of long term sustainable aquaculture.To develop a system of long term sustainable aquaculture.

Need to recapture some of the nutrients from the high cost feed pellets which are not Need to recapture some of the nutrients from the high cost feed pellets which are not converted into prawn biomass.converted into prawn biomass.

Prawn farm effluent contains elevated concentrations of bacteria, phytoplankton, Prawn farm effluent contains elevated concentrations of bacteria, phytoplankton, nutrients and suspended solids, which can potentially adversely affect the water quality nutrients and suspended solids, which can potentially adversely affect the water quality in the receiving waters.in the receiving waters.

Sewage treatment techniques are often ineffective due to the low specific gravity of Sewage treatment techniques are often ineffective due to the low specific gravity of most of the effluent particles, and the high volume and salt content. They are also most of the effluent particles, and the high volume and salt content. They are also prohibitively expensive.prohibitively expensive.

To develop a system of long term sustainable aquaculture.To develop a system of long term sustainable aquaculture.

Page 6: “Biofiltration of Shrimp Pond Effluent by Oysters in a Raceway System”

ObjectivesObjectives

Quantitative determination of the changes in the chemical and biological composition of Quantitative determination of the changes in the chemical and biological composition of prawn farm effluent water after biofiltration by different sizes of the Sydney Rock Oyster prawn farm effluent water after biofiltration by different sizes of the Sydney Rock Oyster ((Saccostrea commercialis)Saccostrea commercialis) .. PhytoplanktonPhytoplankton BacteriaBacteria Nutrients (nitrogen & phosphorus)Nutrients (nitrogen & phosphorus) Total Suspended SolidsTotal Suspended Solids Organic / Inorganic RatioOrganic / Inorganic Ratio

Determine the most efficient system to facilitate maximum filtration by the oysters, by Determine the most efficient system to facilitate maximum filtration by the oysters, by adjusting flow rates and recirculation.adjusting flow rates and recirculation.

Quantitative determination of the changes in the chemical and biological composition of Quantitative determination of the changes in the chemical and biological composition of prawn farm effluent water after biofiltration by different sizes of the Sydney Rock Oyster prawn farm effluent water after biofiltration by different sizes of the Sydney Rock Oyster ((Saccostrea commercialis)Saccostrea commercialis) .. PhytoplanktonPhytoplankton BacteriaBacteria Nutrients (nitrogen & phosphorus)Nutrients (nitrogen & phosphorus) Total Suspended SolidsTotal Suspended Solids Organic / Inorganic RatioOrganic / Inorganic Ratio

Determine the most efficient system to facilitate maximum filtration by the oysters, by Determine the most efficient system to facilitate maximum filtration by the oysters, by adjusting flow rates and recirculation.adjusting flow rates and recirculation.

Page 7: “Biofiltration of Shrimp Pond Effluent by Oysters in a Raceway System”

MethodsMethods

6 raceways stocked with6 raceways stocked withoysters (3 controls and 3 treatmentoysters (3 controls and 3 treatmentreplicates)replicates)

6 raceways stocked with6 raceways stocked withoysters (3 controls and 3 treatmentoysters (3 controls and 3 treatmentreplicates)replicates)

Collect 3 replicate samples Collect 3 replicate samples from each raceway (before and from each raceway (before and after biofiltration) for after biofiltration) for analysis of Chlorophyll analysis of Chlorophyll aa, , bacterial numbers, total bacterial numbers, total suspended solids, & nutrientssuspended solids, & nutrients

Collect 3 replicate samples Collect 3 replicate samples from each raceway (before and from each raceway (before and after biofiltration) for after biofiltration) for analysis of Chlorophyll analysis of Chlorophyll aa, , bacterial numbers, total bacterial numbers, total suspended solids, & nutrientssuspended solids, & nutrients

Effluent from shrimp pondEffluent from shrimp pondEffluent from shrimp pondEffluent from shrimp pond

Shrimp pond waste Shrimp pond waste water pumped at water pumped at constant flow constant flow raterate

Shrimp pond waste Shrimp pond waste water pumped at water pumped at constant flow constant flow raterate

Biofiltered water Biofiltered water released into the released into the environmentenvironment

Biofiltered water Biofiltered water released into the released into the environmentenvironment

Moreton BayMoreton BayMoreton BayMoreton Bay

Biofiltered water Biofiltered water recirculated back recirculated back through the oysters through the oysters for further filteringfor further filtering

Biofiltered water Biofiltered water recirculated back recirculated back through the oysters through the oysters for further filteringfor further filtering

Page 8: “Biofiltration of Shrimp Pond Effluent by Oysters in a Raceway System”

RacewaysRaceways

Page 9: “Biofiltration of Shrimp Pond Effluent by Oysters in a Raceway System”

Raceway SetupRaceway Setup

Page 10: “Biofiltration of Shrimp Pond Effluent by Oysters in a Raceway System”

Effluent Water FlowEffluent Water Flow

Effluent ChannelEffluent ChannelEffluent ChannelEffluent Channel RacewaysRacewaysRacewaysRaceways Recirculating TankRecirculating TankRecirculating TankRecirculating Tank

Moreton BayMoreton BayMoreton BayMoreton Bay

Prawn Prawn PondsPonds

Prawn Prawn PondsPonds

Page 11: “Biofiltration of Shrimp Pond Effluent by Oysters in a Raceway System”

Reduction in bacterial numbers after oyster biofiltrationReduction in bacterial numbers after oyster biofiltration

12%19%

33%

54%

09:00 11:00 13:00 15:000

5

10

15

20

25

30

No.

of

Bac

teri

a pe

r m

l (x

10

)Sampling Time

Inflow Outflow

6

12%19%

33%

54%

09:00 11:00 13:00 15:000

5

10

15

20

25

30

No.

of

Bac

teri

a pe

r m

l (x

10

)Sampling Time

Inflow Outflow

6

Continual FlowContinual Flow RecirculatingRecirculating

Control Oysters0

2

4

6

8

10

12

14

No.

of

Bac

teri

a pe

r m

l (x

10

)

Treatment

Inflow Outflow

37%

108%

6

Control Oysters0

2

4

6

8

10

12

14

No.

of

Bac

teri

a pe

r m

l (x

10

)

Treatment

Inflow Outflow

37%

108%

6N

o. o

f B

acte

ria

per

ml x

106

No.

of

Bac

teri

a pe

r m

l x 1

06

No.

of

Bac

teri

a pe

r m

l x 1

06N

o. o

f B

acte

ria

per

ml x

106

Page 12: “Biofiltration of Shrimp Pond Effluent by Oysters in a Raceway System”

Reduction in chlorophyll a concentration after oyster biofiltration

Reduction in chlorophyll a concentration after oyster biofiltration

54%

09:00 11:00 13:00 15:000

5

10

15

20

25

30

Chl

conc

entr

atio

n (

g/

L)

Sampling Time

Inflow Outflow

115%

20%

4%20%a

54%

09:00 11:00 13:00 15:000

5

10

15

20

25

30

Chl

conc

entr

atio

n (

g/

L)

Sampling Time

Inflow Outflow

115%

20%

4%20%a

Control Oysters

0

5

10

15

20

25

30

35

40

Chl

conc

entr

atio

n (

g/

L)

Treatment

Inflow Outflow

39%

97%

a

Control Oysters0

5

10

15

20

25

30

35

40

Chl

conc

entr

atio

n (

g/

L)

Treatment

Inflow Outflow

39%

97%

a

Continual FlowContinual Flow RecirculatingRecirculating

Chl

a c

once

ntra

tion

g.l-1

)C

hl a

con

cent

rati

on (

µg.

l-1)

Chl

a c

once

ntra

tion

g.l-1

)C

hl a

con

cent

rati

on (

µg.

l-1)

Page 13: “Biofiltration of Shrimp Pond Effluent by Oysters in a Raceway System”

Reduction in Total Suspended Solids after oyster biofiltration

Reduction in Total Suspended Solids after oyster biofiltration

Continual FlowContinual Flow RecirculatingRecirculating

ControlControl OystersOysters00

0.020.020.040.040.060.060.080.08

0.10.10.120.120.140.140.160.16

Tot

al S

uspe

nded

Sol

ids

(g. l

-1)

Tot

al S

uspe

nded

Sol

ids

(g. l

-1)

Treatment Treatment

InflowInflow OutflowOutflow

92%92%76%76%

54%54%

09:0009:00 11:0011:00 13:0013:00 15:0015:0000

0.020.020.040.040.060.060.080.080.10.1

0.120.120.140.140.160.160.180.18

Tot

al S

uspe

nded

Sol

ids

(g. l

-1)

Tot

al S

uspe

nded

Sol

ids

(g. l

-1)

Sampling TimeSampling Time

InflowInflow OutflowOutflow

118%118%

63%63%

32%32%19%19%

Page 14: “Biofiltration of Shrimp Pond Effluent by Oysters in a Raceway System”

Reduction in Total Nutrient Concentrations after oyster biofiltration

Reduction in Total Nutrient Concentrations after oyster biofiltration

NitrogenNitrogen PhosphorusPhosphorus

ControlControl OystersOysters00

0.20.20.40.40.60.60.80.8

111.21.21.41.41.61.61.81.8

22

Tot

al N

itrog

en (

mg.

l-1)

Tot

al N

itrog

en (

mg.

l-1)

Treatment Treatment

InflowInflow OutflowOutflow

89%89%

66%66%

54%54%

ControlControl OystersOysters00

0.020.020.040.040.060.060.080.080.10.1

0.120.120.140.140.160.160.180.180.20.2

Tot

al P

hosp

horu

s (m

g. l-1

)T

otal

Pho

spho

rus

(mg.

l-1)

TreatmentTreatment

InflowInflow OutflowOutflow

85%85%

56%56%

Page 15: “Biofiltration of Shrimp Pond Effluent by Oysters in a Raceway System”

Summary of ResultsSummary of Results

Water qualityWater quality % Reduced after once% Reduced after once % Reduced after % Reduced after threethreeParameter Parameter through the oysters through the oysters times through times through oystersoysters

TSSTSS 35%35% 84%84%

BacteriaBacteria 65%65% 88%88%

Chlorophyll Chlorophyll aa 61%61% 80%80%

NitrogenNitrogen 39%39% ndnd

PhosphorusPhosphorus 44%44% ndnd

Water qualityWater quality % Reduced after once% Reduced after once % Reduced after % Reduced after threethreeParameter Parameter through the oysters through the oysters times through times through oystersoysters

TSSTSS 35%35% 84%84%

BacteriaBacteria 65%65% 88%88%

Chlorophyll Chlorophyll aa 61%61% 80%80%

NitrogenNitrogen 39%39% ndnd

PhosphorusPhosphorus 44%44% ndnd

Page 16: “Biofiltration of Shrimp Pond Effluent by Oysters in a Raceway System”

ConclusionsConclusions Oysters can remove from suspension large quantities of phytoplankton, bacteria, Oysters can remove from suspension large quantities of phytoplankton, bacteria,

nutrients and total suspended solids.nutrients and total suspended solids.

By employing recirculating within the raceway system, the effects of the oysters are By employing recirculating within the raceway system, the effects of the oysters are enhanced significantly.enhanced significantly.

The use of oysters as natural filters of aquaculture effluent has the potential to provide:The use of oysters as natural filters of aquaculture effluent has the potential to provide:

improved water quality of pond effluent and hence reduced environmental impact.improved water quality of pond effluent and hence reduced environmental impact. reduced pond water exchange through recirculation of biotreated water back into the ponds.reduced pond water exchange through recirculation of biotreated water back into the ponds. recapturing of nutrients.recapturing of nutrients. increased profits.increased profits. increased productivity.increased productivity. more productive method of growing and fattening oysters.more productive method of growing and fattening oysters.

Oysters can remove from suspension large quantities of phytoplankton, bacteria, Oysters can remove from suspension large quantities of phytoplankton, bacteria, nutrients and total suspended solids.nutrients and total suspended solids.

By employing recirculating within the raceway system, the effects of the oysters are By employing recirculating within the raceway system, the effects of the oysters are enhanced significantly.enhanced significantly.

The use of oysters as natural filters of aquaculture effluent has the potential to provide:The use of oysters as natural filters of aquaculture effluent has the potential to provide:

improved water quality of pond effluent and hence reduced environmental impact.improved water quality of pond effluent and hence reduced environmental impact. reduced pond water exchange through recirculation of biotreated water back into the ponds.reduced pond water exchange through recirculation of biotreated water back into the ponds. recapturing of nutrients.recapturing of nutrients. increased profits.increased profits. increased productivity.increased productivity. more productive method of growing and fattening oysters.more productive method of growing and fattening oysters.

Page 17: “Biofiltration of Shrimp Pond Effluent by Oysters in a Raceway System”

Continued ResearchContinued Research

Filtration efficiency of oysters at different densities.Filtration efficiency of oysters at different densities.

Test effects of flow rate, versus recirculation of water through the oysters.Test effects of flow rate, versus recirculation of water through the oysters.

Test the efficiency of different sized oysters and determine their growth rate Test the efficiency of different sized oysters and determine their growth rate versus those in control oceanic waters.versus those in control oceanic waters.

Test the ability of macroalgae (Test the ability of macroalgae (Gracilaria edulisGracilaria edulis) to remove dissolved nitrogen ) to remove dissolved nitrogen and phosphorus.and phosphorus.

Undertake commercial scale system at Rocky Point Prawn Farm.Undertake commercial scale system at Rocky Point Prawn Farm.

Filtration efficiency of oysters at different densities.Filtration efficiency of oysters at different densities.

Test effects of flow rate, versus recirculation of water through the oysters.Test effects of flow rate, versus recirculation of water through the oysters.

Test the efficiency of different sized oysters and determine their growth rate Test the efficiency of different sized oysters and determine their growth rate versus those in control oceanic waters.versus those in control oceanic waters.

Test the ability of macroalgae (Test the ability of macroalgae (Gracilaria edulisGracilaria edulis) to remove dissolved nitrogen ) to remove dissolved nitrogen and phosphorus.and phosphorus.

Undertake commercial scale system at Rocky Point Prawn Farm.Undertake commercial scale system at Rocky Point Prawn Farm.

Page 18: “Biofiltration of Shrimp Pond Effluent by Oysters in a Raceway System”

Study AreaStudy Area

RedcliffeRedcliffeRedcliffeRedcliffe

BrisbaneBrisbaneCityCityBrisbaneBrisbaneCityCity

MoretonMoretonIslandIslandMoretonMoretonIslandIsland

NorthNorthNorthNorth

StradbrokeStradbrokeStradbrokeStradbroke

IslandIslandIslandIsland

MoretonMoretonMoretonMoreton

BayBayBayBay

BrisbaneBrisbaneBrisbaneBrisbane

Moreton BayMoreton Bay Prawn FarmPrawn FarmMoreton BayMoreton Bay Prawn FarmPrawn Farm

Page 19: “Biofiltration of Shrimp Pond Effluent by Oysters in a Raceway System”

Pond Design LayoutPond Design Layout

InflowInflowInflowInflow OutflowOutflowOutflowOutflow

SettlingSettlingAreaAreaSettlingSettlingAreaArea

BafflesBafflesto force water up into oyster traysto force water up into oyster trays

BafflesBafflesto force water up into oyster traysto force water up into oyster trays

Baffle to slowBaffle to slowwaterwater

Baffle to slowBaffle to slowwaterwater

MacroalgalMacroalgalAreaAreaMacroalgalMacroalgalAreaArea

Oyster RaftsOyster RaftsOyster RaftsOyster Rafts

Page 20: “Biofiltration of Shrimp Pond Effluent by Oysters in a Raceway System”

Integrated Aquaculture FarmIntegrated Aquaculture Farm

HarvestedHarvestedHarvestedHarvested HarvestedHarvestedHarvestedHarvested HarvestedHarvestedHarvestedHarvested

HarvestedHarvestedHarvestedHarvested

- Faeces- Faeces (organic) (organic) and Ammoniaand Ammonia- Psuedofaeces - Psuedofaeces (inorganic)(inorganic)

- Faeces- Faeces (organic) (organic) and Ammoniaand Ammonia- Psuedofaeces - Psuedofaeces (inorganic)(inorganic)

- Bacteria- Bacteria- Phytoplankton- Phytoplankton- Inorganic particles- Inorganic particles- Detritus- Detritus

- Bacteria- Bacteria- Phytoplankton- Phytoplankton- Inorganic particles- Inorganic particles- Detritus- Detritus

- Faeces- Faeces (organic) (organic) and Ammoniaand Ammonia- Psuedofaeces - Psuedofaeces (inorganic)(inorganic)

- Faeces- Faeces (organic) (organic) and Ammoniaand Ammonia- Psuedofaeces - Psuedofaeces (inorganic)(inorganic)

HarvestedHarvestedHarvestedHarvested

Nutrient richNutrient richfood source - food source - high in aminohigh in aminoacidsacids

Nutrient richNutrient richfood source - food source - high in aminohigh in aminoacidsacidsShrimpShrimpShrimpShrimp

OysterOysterOysterOyster

ClamClamClamClam AbaloneAbaloneAbaloneAbalone

MacroalgaeMacroalgaeMacroalgaeMacroalgae


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